Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0006_P20
(446 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g57050.3 68416.m06352 cystathionine beta-lyase, chloroplast /... 57 6e-09
At3g57050.2 68416.m06351 cystathionine beta-lyase, chloroplast /... 57 6e-09
At3g57050.1 68416.m06350 cystathionine beta-lyase, chloroplast /... 57 6e-09
At1g64660.1 68414.m07330 Cys/Met metabolism pyridoxal-phosphate-... 41 3e-04
At3g01120.1 68416.m00016 cystathionine gamma-synthase, chloropla... 41 4e-04
At1g33320.1 68414.m04121 cystathionine gamma-synthase, chloropla... 36 0.009
At4g24280.1 68417.m03486 heat shock protein 70, putative / HSP70... 30 0.82
At5g06060.1 68418.m00671 tropinone reductase, putative / tropine... 29 1.9
At5g58210.3 68418.m07288 hydroxyproline-rich glycoprotein family... 28 3.3
At5g58210.2 68418.m07287 hydroxyproline-rich glycoprotein family... 28 3.3
At5g58210.1 68418.m07286 hydroxyproline-rich glycoprotein family... 28 3.3
At3g25410.1 68416.m03160 bile acid:sodium symporter family prote... 28 3.3
At5g36150.1 68418.m04356 pentacyclic triterpene synthase, putati... 27 4.4
At2g02580.1 68415.m00198 cytochrome P450 family protein 27 5.8
At1g80530.1 68414.m09439 nodulin family protein similar to nodul... 27 7.6
At1g66730.1 68414.m07585 ATP dependent DNA ligase family protein... 27 7.6
>At3g57050.3 68416.m06352 cystathionine beta-lyase, chloroplast /
beta-cystathionase / cysteine lyase (CBL) identical to
SP|P53780 Cystathionine beta-lyase, chloroplast
precursor (EC 4.4.1.8) (CBL) (Beta-cystathionase)
(Cysteine lyase) {Arabidopsis thaliana}
Length = 378
Score = 56.8 bits (131), Expect = 6e-09
Identities = 25/56 (44%), Positives = 36/56 (64%)
Frame = +3
Query: 276 LAALEGGKHALVFASGLGATTTIASLLSKGDHILSSDDVYGGTNRLLRQVVSRMGI 443
LA L+ A F SG+ A + + L+ G+ I++ DDVYGG++RLL QVV R G+
Sbjct: 142 LAKLDKADRAFCFTSGMAALSAVTHLIKNGEEIVAGDDVYGGSDRLLSQVVPRSGV 197
Score = 41.1 bits (92), Expect = 3e-04
Identities = 20/63 (31%), Positives = 35/63 (55%)
Frame = +2
Query: 86 LKPKTGFATQAIHAGQDPDKWDSAAVVAPIVTSTTFKQTAPTVHNGYEYGRSGNPTRNTL 265
+K + +T ++ D +D A+ P+ + TFKQ + + Y+Y RSGNPTR+ L
Sbjct: 81 IKEEASVSTLLVNLDNKFDPFD--AMSTPLYQTATFKQPSAIENGPYDYTRSGNPTRDAL 138
Query: 266 DRM 274
+ +
Sbjct: 139 ESL 141
>At3g57050.2 68416.m06351 cystathionine beta-lyase, chloroplast /
beta-cystathionase / cysteine lyase (CBL) identical to
SP|P53780 Cystathionine beta-lyase, chloroplast
precursor (EC 4.4.1.8) (CBL) (Beta-cystathionase)
(Cysteine lyase) {Arabidopsis thaliana}
Length = 449
Score = 56.8 bits (131), Expect = 6e-09
Identities = 25/56 (44%), Positives = 36/56 (64%)
Frame = +3
Query: 276 LAALEGGKHALVFASGLGATTTIASLLSKGDHILSSDDVYGGTNRLLRQVVSRMGI 443
LA L+ A F SG+ A + + L+ G+ I++ DDVYGG++RLL QVV R G+
Sbjct: 127 LAKLDKADRAFCFTSGMAALSAVTHLIKNGEEIVAGDDVYGGSDRLLSQVVPRSGV 182
Score = 41.1 bits (92), Expect = 3e-04
Identities = 20/63 (31%), Positives = 35/63 (55%)
Frame = +2
Query: 86 LKPKTGFATQAIHAGQDPDKWDSAAVVAPIVTSTTFKQTAPTVHNGYEYGRSGNPTRNTL 265
+K + +T ++ D +D A+ P+ + TFKQ + + Y+Y RSGNPTR+ L
Sbjct: 66 IKEEASVSTLLVNLDNKFDPFD--AMSTPLYQTATFKQPSAIENGPYDYTRSGNPTRDAL 123
Query: 266 DRM 274
+ +
Sbjct: 124 ESL 126
>At3g57050.1 68416.m06350 cystathionine beta-lyase, chloroplast /
beta-cystathionase / cysteine lyase (CBL) identical to
SP|P53780 Cystathionine beta-lyase, chloroplast
precursor (EC 4.4.1.8) (CBL) (Beta-cystathionase)
(Cysteine lyase) {Arabidopsis thaliana}
Length = 464
Score = 56.8 bits (131), Expect = 6e-09
Identities = 25/56 (44%), Positives = 36/56 (64%)
Frame = +3
Query: 276 LAALEGGKHALVFASGLGATTTIASLLSKGDHILSSDDVYGGTNRLLRQVVSRMGI 443
LA L+ A F SG+ A + + L+ G+ I++ DDVYGG++RLL QVV R G+
Sbjct: 142 LAKLDKADRAFCFTSGMAALSAVTHLIKNGEEIVAGDDVYGGSDRLLSQVVPRSGV 197
Score = 41.1 bits (92), Expect = 3e-04
Identities = 20/63 (31%), Positives = 35/63 (55%)
Frame = +2
Query: 86 LKPKTGFATQAIHAGQDPDKWDSAAVVAPIVTSTTFKQTAPTVHNGYEYGRSGNPTRNTL 265
+K + +T ++ D +D A+ P+ + TFKQ + + Y+Y RSGNPTR+ L
Sbjct: 81 IKEEASVSTLLVNLDNKFDPFD--AMSTPLYQTATFKQPSAIENGPYDYTRSGNPTRDAL 138
Query: 266 DRM 274
+ +
Sbjct: 139 ESL 141
>At1g64660.1 68414.m07330 Cys/Met metabolism
pyridoxal-phosphate-dependent enzyme family protein
similar to SP|P13254 Methionine gamma-lyase (EC
4.4.1.11) (L-methioninase) {Pseudomonas putida};
contains Pfam profile PF01053: Cys/Met metabolism
PLP-dependent enzyme
Length = 441
Score = 41.1 bits (92), Expect = 3e-04
Identities = 20/54 (37%), Positives = 34/54 (62%), Gaps = 1/54 (1%)
Frame = +3
Query: 276 LAALEGGKHALVFASGLGATTTIA-SLLSKGDHILSSDDVYGGTNRLLRQVVSR 434
+AALEG + A +SG+ A +++ L S G H++++ +YGGT+ LL + R
Sbjct: 110 MAALEGTQAAYCTSSGMSAISSVMLQLCSSGGHVVAASTLYGGTHALLSHFLPR 163
>At3g01120.1 68416.m00016 cystathionine gamma-synthase, chloroplast
/ O-succinylhomoserine (Thiol)-lyase (CGS) identical to
SP|P55217 Cystathionine gamma-synthase, chloroplast
precursor (EC 4.2.99.9) (CGS) (O-succinylhomoserine
(Thiol)-lyase) {Arabidopsis thaliana}
Length = 563
Score = 40.7 bits (91), Expect = 4e-04
Identities = 20/57 (35%), Positives = 35/57 (61%), Gaps = 1/57 (1%)
Frame = +3
Query: 276 LAALEGGKHALVFASGLGATTT-IASLLSKGDHILSSDDVYGGTNRLLRQVVSRMGI 443
++ALEG + LV ASG+ A+T + +L+ G HI+++ D Y T + + ++GI
Sbjct: 241 ISALEGAESTLVMASGMCASTVMLLALVPAGGHIVTTTDCYRKTRIFMENFLPKLGI 297
Score = 29.1 bits (62), Expect = 1.4
Identities = 21/70 (30%), Positives = 35/70 (50%), Gaps = 9/70 (12%)
Frame = +2
Query: 119 IHAGQDPDKWD-SAAVVAPIVTSTT--FKQTAPTVHN------GYEYGRSGNPTRNTLDR 271
+HAG+ + + A+ P+V ++ FK+TA + +EYGR GNPT L+
Sbjct: 180 VHAGERLGRGIVTDAITTPVVNTSAYFFKKTAELIDFKEKRSVSFEYGRYGNPTTVVLED 239
Query: 272 MPRSFGGRET 301
+ G E+
Sbjct: 240 KISALEGAES 249
>At1g33320.1 68414.m04121 cystathionine gamma-synthase, chloroplast,
putative / O-succinylhomoserine (Thiol)-lyase, putative
strong similarity to SP|P55217 Cystathionine
gamma-synthase, chloroplast precursor (EC 4.2.99.9)
(CGS) (O-succinylhomoserine (Thiol)-lyase) {Arabidopsis
thaliana}; contains Pfam profile PF01053: Cys/Met
metabolism PLP-dependent enzyme
Length = 412
Score = 36.3 bits (80), Expect = 0.009
Identities = 18/59 (30%), Positives = 34/59 (57%), Gaps = 1/59 (1%)
Frame = +3
Query: 270 ECLAALEGGKHALVFASGLGATTT-IASLLSKGDHILSSDDVYGGTNRLLRQVVSRMGI 443
E ++ LEG + LV ASG+ A+ + +L+ HI+++ D Y T + ++++GI
Sbjct: 89 EKISVLEGAESTLVMASGMYASNVMLLALVPTNGHIVATKDCYKETRIFMENFLTKLGI 147
>At4g24280.1 68417.m03486 heat shock protein 70, putative / HSP70,
putative strong similarity to heat shock protein 70
[Arabidopsis thaliana] GI:6746592; similar to heat shock
70 protein - Spinacia oleracea,PID:g2654208
Length = 718
Score = 29.9 bits (64), Expect = 0.82
Identities = 18/53 (33%), Positives = 29/53 (54%), Gaps = 1/53 (1%)
Frame = +3
Query: 222 VMNTEDLEIRLGTL*TECLAALEGGKHALVF-ASGLGATTTIASLLSKGDHIL 377
V+N + + I LGT +AA+EGGK +V A G T ++ + GD ++
Sbjct: 75 VVNEKVVGIDLGTT-NSAVAAMEGGKPTIVTNAEGQRTTPSVVAYTKSGDRLV 126
>At5g06060.1 68418.m00671 tropinone reductase, putative / tropine
dehydrogenase, putative similar to tropinone reductase
SP:P50165 from [Datura stramonium]
Length = 264
Score = 28.7 bits (61), Expect = 1.9
Identities = 17/49 (34%), Positives = 26/49 (53%)
Frame = +3
Query: 300 HALVFASGLGATTTIASLLSKGDHILSSDDVYGGTNRLLRQVVSRMGID 446
H L+ ASG+G+ I+S+ G LSS +YG T L Q+ + +
Sbjct: 132 HPLLKASGVGSIVFISSVA--GLVHLSSGSIYGATKGALNQLTRNLACE 178
>At5g58210.3 68418.m07288 hydroxyproline-rich glycoprotein family
protein
Length = 380
Score = 27.9 bits (59), Expect = 3.3
Identities = 18/55 (32%), Positives = 30/55 (54%), Gaps = 3/55 (5%)
Frame = -2
Query: 442 IPILDTTCLNNLF--VPPYTSSE-DKM*SPLLSSEAIVVVAPRPEAKTKACFPPS 287
+PI++ L+ + VP TSS P++ +EA+ VV+P A T + F P+
Sbjct: 160 VPIVEAKALSEVSPSVPADTSSHFSPAPVPIVEAEALSVVSPSVPADTSSHFSPA 214
>At5g58210.2 68418.m07287 hydroxyproline-rich glycoprotein family
protein
Length = 380
Score = 27.9 bits (59), Expect = 3.3
Identities = 18/55 (32%), Positives = 30/55 (54%), Gaps = 3/55 (5%)
Frame = -2
Query: 442 IPILDTTCLNNLF--VPPYTSSE-DKM*SPLLSSEAIVVVAPRPEAKTKACFPPS 287
+PI++ L+ + VP TSS P++ +EA+ VV+P A T + F P+
Sbjct: 160 VPIVEAKALSEVSPSVPADTSSHFSPAPVPIVEAEALSVVSPSVPADTSSHFSPA 214
>At5g58210.1 68418.m07286 hydroxyproline-rich glycoprotein family
protein
Length = 380
Score = 27.9 bits (59), Expect = 3.3
Identities = 18/55 (32%), Positives = 30/55 (54%), Gaps = 3/55 (5%)
Frame = -2
Query: 442 IPILDTTCLNNLF--VPPYTSSE-DKM*SPLLSSEAIVVVAPRPEAKTKACFPPS 287
+PI++ L+ + VP TSS P++ +EA+ VV+P A T + F P+
Sbjct: 160 VPIVEAKALSEVSPSVPADTSSHFSPAPVPIVEAEALSVVSPSVPADTSSHFSPA 214
>At3g25410.1 68416.m03160 bile acid:sodium symporter family protein
low similarity to SP|Q14973 Sodium/bile acid
cotransporter (Na(+)/bile acid cotransporter) {Homo
sapiens}; contains Pfam profile PF01758: Sodium Bile
acid symporter family
Length = 431
Score = 27.9 bits (59), Expect = 3.3
Identities = 12/27 (44%), Positives = 19/27 (70%), Gaps = 1/27 (3%)
Frame = -2
Query: 151 IPFIRILTSVNSLSRKPSF-WLQKSVF 74
+PF+ LT+V +LS PSF W+ K ++
Sbjct: 115 LPFVVALTAVAALSYPPSFTWVSKDLY 141
>At5g36150.1 68418.m04356 pentacyclic triterpene synthase, putative
similar to pentacyclic triterpene synthase [gi:6650208]
[PMID:11247608]; oxidosqualene cyclase; also highly
similar to beta-amyrin synthase, lupeol synthase,
cycloartenol synthase
Length = 729
Score = 27.5 bits (58), Expect = 4.4
Identities = 12/38 (31%), Positives = 19/38 (50%)
Frame = +2
Query: 146 WDSAAVVAPIVTSTTFKQTAPTVHNGYEYGRSGNPTRN 259
WD+A V+ ++ + + PT+ GY Y R T N
Sbjct: 419 WDTAFVLQVMLAADVDDEIRPTLIKGYSYLRKSQFTEN 456
>At2g02580.1 68415.m00198 cytochrome P450 family protein
Length = 500
Score = 27.1 bits (57), Expect = 5.8
Identities = 13/44 (29%), Positives = 25/44 (56%), Gaps = 1/44 (2%)
Frame = +2
Query: 44 RDALTEYKMSEDGF-LKPKTGFATQAIHAGQDPDKWDSAAVVAP 172
R+ ++E++++ G+ ++PKT G+DPD W A + P
Sbjct: 372 REVMSEFEIN--GYKIQPKTLLYVNVWAIGRDPDSWKDADMFYP 413
>At1g80530.1 68414.m09439 nodulin family protein similar to
nodulin-like protein [Arabidopsis thaliana] GI:3329368,
nodule-specific protein Nlj70 [Lotus japonicus]
GI:3329366
Length = 561
Score = 26.6 bits (56), Expect = 7.6
Identities = 15/52 (28%), Positives = 24/52 (46%)
Frame = +2
Query: 239 SGNPTRNTLDRMPRSFGGRETRFGLCLWSWSYHYNRFTTQQRGSHLVLGRCI 394
SG N L ++ +FG ++T LCL+S+ R + H V R +
Sbjct: 360 SGVTVSNNLAQIGFAFGIKDTTILLCLFSFFNFIGRLASGAISEHFVRSRTL 411
>At1g66730.1 68414.m07585 ATP dependent DNA ligase family protein
contains Pfam profile: PF01068 ATP dependent DNA ligase
domain
Length = 1417
Score = 26.6 bits (56), Expect = 7.6
Identities = 17/46 (36%), Positives = 22/46 (47%)
Frame = +2
Query: 29 IVNDLRDALTEYKMSEDGFLKPKTGFATQAIHAGQDPDKWDSAAVV 166
+ ND D + E+ SED L K + G+ PD W SA VV
Sbjct: 1308 LFNDETDGMKEF-YSEDKILAKKPPY----YRTGETPDMWFSAEVV 1348
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,285,938
Number of Sequences: 28952
Number of extensions: 178725
Number of successful extensions: 452
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 439
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 446
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 722638680
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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