Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0006_O07
(496 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 225 2e-60
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 126 2e-30
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 122 4e-29
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 118 5e-28
SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharom... 29 0.29
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 1.6
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 2.1
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 2.1
SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |S... 25 4.7
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 25 4.7
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 4.7
SPBC3B8.04c |||membrane transporter|Schizosaccharomyces pombe|ch... 25 8.3
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 225 bits (551), Expect = 2e-60
Identities = 95/128 (74%), Positives = 112/128 (87%)
Frame = +2
Query: 2 GQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVPRAILVDLE 181
GQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYVPRA+LVDLE
Sbjct: 10 GQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYVPRAVLVDLE 69
Query: 182 PGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDC 361
PGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVVR+E+E+CD
Sbjct: 70 PGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDA 129
Query: 362 LPGFQLTH 385
L GFQLTH
Sbjct: 130 LQGFQLTH 137
Score = 35.1 bits (77), Expect = 0.006
Identities = 14/21 (66%), Positives = 18/21 (85%)
Frame = +1
Query: 430 ISXIREEYPDRIMNTYSVVPS 492
+S IREEYPDR+M T+SV P+
Sbjct: 152 LSKIREEYPDRMMATFSVAPA 172
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 126 bits (303), Expect = 2e-30
Identities = 57/130 (43%), Positives = 81/130 (62%), Gaps = 2/130 (1%)
Frame = +2
Query: 2 GQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGKYVPRAILVD 175
GQ G QIG WE+ EHGI P G + ++ Q + +++E GKYVPR+I VD
Sbjct: 10 GQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIYVD 69
Query: 176 LEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESC 355
LEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D +R+ +++C
Sbjct: 70 LEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNC 129
Query: 356 DCLPGFQLTH 385
L GF + H
Sbjct: 130 SGLQGFLVFH 139
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 122 bits (293), Expect = 4e-29
Identities = 56/134 (41%), Positives = 82/134 (61%), Gaps = 6/134 (4%)
Frame = +2
Query: 2 GQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEASGGKYVPRA 163
GQ G QIG WE+ EHGI P G H ++ + +++E GK+VPR+
Sbjct: 10 GQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSETGQGKFVPRS 69
Query: 164 ILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKE 343
I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++DSVL+ +R+
Sbjct: 70 IYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRM 129
Query: 344 SESCDCLPGFQLTH 385
+++C L GF + H
Sbjct: 130 ADNCSGLQGFLVFH 143
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 118 bits (284), Expect = 5e-28
Identities = 49/130 (37%), Positives = 85/130 (65%), Gaps = 2/130 (1%)
Frame = +2
Query: 2 GQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVPRAILVDLE 181
GQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+PRAIL+DLE
Sbjct: 11 GQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIPRAILIDLE 70
Query: 182 PGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDVVRKESESC 355
P ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D++ +E++
Sbjct: 71 PRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREADGS 129
Query: 356 DCLPGFQLTH 385
D L GF L H
Sbjct: 130 DSLEGFSLLH 139
Score = 25.4 bits (53), Expect = 4.7
Identities = 8/22 (36%), Positives = 15/22 (68%)
Frame = +1
Query: 430 ISXIREEYPDRIMNTYSVVPSA 495
+ + + YP +I+ TYSV P++
Sbjct: 154 LERLNDRYPKKIIQTYSVFPNS 175
>SPBC354.13 |rga6||GTPase activating protein
Rga6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 733
Score = 29.5 bits (63), Expect = 0.29
Identities = 16/42 (38%), Positives = 26/42 (61%)
Frame = +2
Query: 365 PGFQLTHFSSEAVPAPVWAHYLSXRSVKSTPTES*THTQSSP 490
P +++ S AVP+ +A+ +S RSV S T+S T++SP
Sbjct: 637 PQPKISTVSDTAVPSMSFANNISSRSVISAATDSKPSTRTSP 678
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 1.6
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +2
Query: 14 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 115
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 26.6 bits (56), Expect = 2.1
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = +2
Query: 278 AKGHYTEGAELVDSVLDVVRKESES 352
A+GH G ELV + D +RK+SE+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSEN 207
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 2.1
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +2
Query: 209 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 310
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 25.4 bits (53), Expect = 4.7
Identities = 19/71 (26%), Positives = 33/71 (46%), Gaps = 1/71 (1%)
Frame = +3
Query: 204 VRDLSDRYSALTTLFSDSPAPVTTGPRDTTQR-ALS*STQFLT*SEKNPNHVIVYQDSNS 380
+ D +R+ L F SP+ +T P+ + LS S +++ S ++P+H V S+
Sbjct: 27 ISDSPNRHHNLVDAFMQSPS-YSTQPKSAVEPLGLSFSPGYISPSSQSPHHGPVRSPSSR 85
Query: 381 HTSPRRRYRLR 413
P R R
Sbjct: 86 KPLPASPSRTR 96
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.4 bits (53), Expect = 4.7
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +2
Query: 230 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDC 361
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 25.4 bits (53), Expect = 4.7
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -2
Query: 267 PAPDCPKTKLSGRNICPKGPERTESMVPGSRS 172
P+ PK L R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
>SPBC3B8.04c |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 867
Score = 24.6 bits (51), Expect = 8.3
Identities = 13/31 (41%), Positives = 15/31 (48%)
Frame = -2
Query: 234 GRNICPKGPERTESMVPGSRSTRMARGTYLP 142
G NI PER + +S RMAR LP
Sbjct: 106 GNNINYPTPERLQKSSASRKSGRMARSQELP 136
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,833,787
Number of Sequences: 5004
Number of extensions: 34255
Number of successful extensions: 106
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 99
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 103
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 194131776
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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