Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0006_O01
(549 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2 (e... 91 3e-19
At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF... 90 1e-18
At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1 (e... 87 7e-18
At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id... 86 2e-17
At2g40660.1 68415.m05017 tRNA-binding region domain-containing p... 32 0.22
At5g08415.1 68418.m00991 lipoic acid synthase family protein sim... 30 1.2
At2g36190.1 68415.m04442 beta-fructosidase, putative / beta-fruc... 28 3.6
At2g05170.1 68415.m00544 vacuolar protein sorting 11 family prot... 27 6.2
>At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2
(eEF1Balpha2) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686821
Length = 224
Score = 91.5 bits (217), Expect = 3e-19
Identities = 52/173 (30%), Positives = 79/173 (45%), Gaps = 3/173 (1%)
Frame = +2
Query: 38 MALGDVKTAQGLNELNQYLAERSYVSGYTPSQADIKVFEQVGKVPAASLPHVLRWYSHIA 217
+ D+ T +G+ + ++LA ++Y+SG S D+KV+ V P+ + P+ +WY +A
Sbjct: 3 VTFSDLHTEEGVKSVEEHLAGKTYISGDQLSVDDVKVYAAVPVKPSDAFPNASKWYESVA 62
Query: 218 SYTPAERKTWSEGVSXXXXXXXXXXXXXXSNXXXXXXXX---LFGSGXXXXXXXXXXXXX 388
S + GV + LFG
Sbjct: 63 SQLAKSFPGKAVGVQFGGSAAAAPAVEAEAPAAAADDDDDMDLFGDETEEEKKAAEE--- 119
Query: 389 XXLKAYADXKSKKPALIAKSSIILDVKPWDDETDMKEMENQVRTIEMDGLLWG 547
+ A +KKP KSS+++DVKPWDDETDMK++E VR +EM GL WG
Sbjct: 120 ---REAAKKDTKKPKESGKSSVLMDVKPWDDETDMKKLEEAVRGVEMPGLFWG 169
>At2g18110.1 68415.m02105 elongation factor 1-beta, putative /
EF-1-beta, putative nearly identical to eEF-1beta
[Arabidopsis thaliana] GI:398606
Length = 231
Score = 89.8 bits (213), Expect = 1e-18
Identities = 55/175 (31%), Positives = 80/175 (45%), Gaps = 6/175 (3%)
Frame = +2
Query: 41 ALGDVKTAQGLNELNQYLAERSYVSGYTPSQADIKVFEQVGKVPAASLPHVLRWYSHI-- 214
A ++ + GL +L+++L RSY++GY S+ DI VF + K P + +V RW++HI
Sbjct: 3 AFPNLNSGSGLKKLDEHLLTRSYITGYQASKDDITVFTALSKPPTSEFVNVSRWFNHIDA 62
Query: 215 ---ASYTPAERK-TWSEGVSXXXXXXXXXXXXXXSNXXXXXXXXLFGSGXXXXXXXXXXX 382
S AE EG S S
Sbjct: 63 LLRISGVSAEGSGVIVEGSSPITEEAVATPPAADSKDTAAEEEDDDDVDLFGEETEEEKK 122
Query: 383 XXXXLKAYADXKSKKPALIAKSSIILDVKPWDDETDMKEMENQVRTIEMDGLLWG 547
A +KK KSS+++D+KPWDDETDMK++E VR+I+M+GL WG
Sbjct: 123 AAEERAASVKASTKKKES-GKSSVLMDIKPWDDETDMKKLEEAVRSIQMEGLFWG 176
>At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1
(eEF1Balpha1) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686819
Length = 228
Score = 87.0 bits (206), Expect = 7e-18
Identities = 50/171 (29%), Positives = 78/171 (45%), Gaps = 1/171 (0%)
Frame = +2
Query: 38 MALGDVKTAQGLNELNQYLAERSYVSGYTPSQADIKVFEQVGKVPAASLPHVLRWYSHIA 217
+ D+ T +GL L ++LA ++Y+SG S D+KV+ V + P P+ +WY +A
Sbjct: 3 VTFSDLHTERGLKTLEEHLAGKTYISGDQLSVDDVKVYAAVLENPGDGFPNASKWYDSVA 62
Query: 218 SYTPAERKTWSEGVSXXXXXXXXXXXXXXSNXXXXXXXXLFGSGXXXXXXXXXXXXXXXL 397
S+ ++GV +
Sbjct: 63 SHLAKSFPGKADGVRVGGGVAPPSEAHPHTEEPAADGDGDDDDDIDLFADETEDEKKAAE 122
Query: 398 KAYADXK-SKKPALIAKSSIILDVKPWDDETDMKEMENQVRTIEMDGLLWG 547
+ A K +KK KSS++L+VKPWDDETDMK++E VR+++M GL WG
Sbjct: 123 EREAAKKDTKKTKESGKSSVLLEVKPWDDETDMKKLEEAVRSVQMPGLTWG 173
>At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta
identical to SP|P48006 Elongation factor 1-beta
(EF-1-beta) {Arabidopsis thaliana}
Length = 231
Score = 85.8 bits (203), Expect = 2e-17
Identities = 50/175 (28%), Positives = 80/175 (45%), Gaps = 6/175 (3%)
Frame = +2
Query: 41 ALGDVKTAQGLNELNQYLAERSYVSGYTPSQADIKVFEQVGKVPAASLPHVLRWYSHIAS 220
A ++ + GL +L+++L RSY++GY S+ DI VF + K P + + RWY+HI +
Sbjct: 3 AFPNLNSDAGLKKLDEHLLTRSYITGYQASKDDITVFAALAKPPTSQYVNASRWYNHIDA 62
Query: 221 YTPAERKTWSEGVSXXXXXXXXXXXXXXSNXXXXXXXXLFGSGXXXXXXXXXXXXXXXLK 400
+ +EG + K
Sbjct: 63 LLRISGVS-AEGSGVIVEGSAPITEEAVATPPAADSKDAAADEEDDDDVDLFGEETEEEK 121
Query: 401 AYADXK------SKKPALIAKSSIILDVKPWDDETDMKEMENQVRTIEMDGLLWG 547
A+ + S K KSS+++D+KPWDDETDMK++E V++I+M+GL WG
Sbjct: 122 KAAEERAASVKASTKKKESGKSSVLIDIKPWDDETDMKKLEEAVKSIQMEGLFWG 176
>At2g40660.1 68415.m05017 tRNA-binding region domain-containing
protein similar to SP|Q12904 Multisynthetase complex
auxiliary component p43 [Contains: Endothelial-monocyte
activating polypeptide II (EMAP-II) (Small inducible
cytokine subfamily E member 1)] {Homo sapiens}; contains
Pfam profile PF01588: Putative tRNA binding domain
Length = 389
Score = 32.3 bits (70), Expect = 0.22
Identities = 22/57 (38%), Positives = 32/57 (56%), Gaps = 9/57 (15%)
Frame = +2
Query: 71 LNELNQYLAERSYV--SGYTPSQADIKVFEQV-GKVPAAS------LPHVLRWYSHI 214
L +LN LA +S + +G TPS AD+ VF + V S +PHV+RW ++I
Sbjct: 76 LEKLNLELATKSVLLGNGLTPSAADVAVFSALHSSVLGLSDSDKEKVPHVIRWVNYI 132
>At5g08415.1 68418.m00991 lipoic acid synthase family protein
similar to lipoic acid synthase from Arabidopsis
thaliana [gi:3928758], from Mus musculus [gi:14669826]
Pfam profile PF04055: radical SAM domain protein
Length = 394
Score = 29.9 bits (64), Expect = 1.2
Identities = 17/44 (38%), Positives = 26/44 (59%)
Frame = +2
Query: 416 KSKKPALIAKSSIILDVKPWDDETDMKEMENQVRTIEMDGLLWG 547
K KP +I K+SI+L + D+E +KE +R I++D L G
Sbjct: 287 KISKPGMITKTSIMLGLGETDEE--LKEAMADLRAIDVDILTLG 328
>At2g36190.1 68415.m04442 beta-fructosidase, putative /
beta-fructofuranosidase, putative similar to
beta-fructofuranosidase GI:18324 from [Daucus carota]
Length = 591
Score = 28.3 bits (60), Expect = 3.6
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = +2
Query: 71 LNELNQYLAERSYVSGYTPSQADIKVFEQVGKVPAASL 184
+N + + +R V G TP+QAD++V VG + A +
Sbjct: 386 MNNHDIKMGQRIEVKGITPAQADVEVTFYVGSLEKAEI 423
>At2g05170.1 68415.m00544 vacuolar protein sorting 11 family protein
/ VPS11 family protein similar to Vacuolar protein
sorting 11 (hVPS11) (PP3476) (Swiss-Prot:Q9H270) [Homo
sapiens]; similar to Vacuolar biogenesis protein END1
(PEP5 protein) (Vacuolar protein sorting 11)
(Swiss-Prot:P12868) [Saccharomyces cerevisiae]
Length = 932
Score = 27.5 bits (58), Expect = 6.2
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = +2
Query: 71 LNELNQYLAERSYVSGYTPSQADIKVFEQVGKV 169
L +L Y YVS PSQA + + EQ GK+
Sbjct: 495 LEDLGNYDEALQYVSSLEPSQAGVTI-EQYGKI 526
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,255,146
Number of Sequences: 28952
Number of extensions: 173561
Number of successful extensions: 420
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 410
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 419
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1033331880
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -