Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0006_N09
(547 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g73180.1 68414.m08469 eukaryotic translation initiation facto... 54 5e-08
At5g27640.1 68418.m03311 eukaryotic translation initiation facto... 39 0.003
At5g25780.1 68418.m03060 eukaryotic translation initiation facto... 35 0.041
At1g62440.1 68414.m07044 leucine-rich repeat family protein / ex... 29 2.0
At3g51340.1 68416.m05620 aspartyl protease family protein contai... 29 2.7
At5g44340.1 68418.m05429 tubulin beta-4 chain (TUB4) nearly iden... 28 3.5
At4g31160.1 68417.m04423 transducin family protein / WD-40 repea... 28 3.5
At4g38560.1 68417.m05459 expressed protein 28 4.7
At3g19020.1 68416.m02415 leucine-rich repeat family protein / ex... 28 4.7
At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein ... 27 6.2
At5g43020.1 68418.m05248 leucine-rich repeat transmembrane prote... 27 6.2
At5g32590.1 68418.m03867 myosin heavy chain-related similar to M... 27 6.2
At1g20130.1 68414.m02518 family II extracellular lipase, putativ... 27 6.2
At5g53870.1 68418.m06701 plastocyanin-like domain-containing pro... 27 8.2
At5g04560.1 68418.m00456 DEMETER protein (DME) identical to DEME... 27 8.2
At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest ... 27 8.2
At4g10750.1 68417.m01755 HpcH/HpaI aldolase family protein simil... 27 8.2
At1g28290.1 68414.m03472 pollen Ole e 1 allergen and extensin fa... 27 8.2
>At1g73180.1 68414.m08469 eukaryotic translation initiation
factor-related similar to eukaryotic translation
initiation factor 2A (GI:21956484) [Homo sapiens];
similar to Eukaryotic translation initiation factor 3
subunit 9 (eIF-3 eta) (eIF3 p116) (eIF3 p110) (eIF3b)
(Swiss-Prot:P55884) [Homo sapiens]
Length = 513
Score = 54.4 bits (125), Expect = 5e-08
Identities = 27/77 (35%), Positives = 40/77 (51%)
Frame = +2
Query: 5 GHIEVWDTRGYKKLGACSAPDTTCLQWDPRGETFFTATTYPRLTVGNGYKIWHYTGVLMH 184
G + WD K+LG+ A + +W P G F TA+T PR + NG KI++Y G +
Sbjct: 338 GDMAFWDVVNKKQLGSNKAEWSVTSEWSPDGRYFLTASTAPRRQIDNGMKIFNYDGKRYY 397
Query: 185 KRVCQEKENLLGINWQP 235
K++ E L W+P
Sbjct: 398 KKMF---ERLYQAEWKP 411
>At5g27640.1 68418.m03311 eukaryotic translation initiation factor 3
subunit 9 / eIF-3 eta / eIF3b (TIF3B1) nearly identical
to SP|Q9C5Z1 Eukaryotic translation initiation factor 3
subunit 9 (eIF-3 eta) (eIF3 p110) (eIF3b) {Arabidopsis
thaliana}
Length = 712
Score = 38.7 bits (86), Expect = 0.003
Identities = 18/78 (23%), Positives = 39/78 (50%)
Frame = +2
Query: 2 SGHIEVWDTRGYKKLGACSAPDTTCLQWDPRGETFFTATTYPRLTVGNGYKIWHYTGVLM 181
+G +E ++ + + T ++WDP G TA T + NG+ IW + G+++
Sbjct: 533 NGQLEFFNVDELETMATAEHFMATDIEWDPTGRYVATAVTSVH-EMENGFTIWSFNGIML 591
Query: 182 HKRVCQEKENLLGINWQP 235
++ + K++ + W+P
Sbjct: 592 YRIL---KDHFFQLAWRP 606
>At5g25780.1 68418.m03060 eukaryotic translation initiation factor 3
subunit 9, putative / eIF-3 eta, putative / eIF3b,
putative nearly identical to SP|Q9C5Z1 Eukaryotic
translation initiation factor 3 subunit 9 (eIF-3 eta)
(eIF3 p110) (eIF3b) {Arabidopsis thaliana}
Length = 714
Score = 34.7 bits (76), Expect = 0.041
Identities = 17/78 (21%), Positives = 38/78 (48%)
Frame = +2
Query: 2 SGHIEVWDTRGYKKLGACSAPDTTCLQWDPRGETFFTATTYPRLTVGNGYKIWHYTGVLM 181
+G +E ++ + + T ++WDP G T+ T + NG+ IW + G ++
Sbjct: 534 NGQLEFFNVDELETMATAEHFMATDIEWDPTGRYVATSVTTVH-EMENGFTIWSFNGNMV 592
Query: 182 HKRVCQEKENLLGINWQP 235
++ + K++ + W+P
Sbjct: 593 YRIL---KDHFFQLAWRP 607
>At1g62440.1 68414.m07044 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 826
Score = 29.1 bits (62), Expect = 2.0
Identities = 12/39 (30%), Positives = 19/39 (48%)
Frame = +2
Query: 281 IEDATPKPAGAYRPPGARNKPSNFTLHEHEKPHKPGESP 397
+ + P P+ Y PP ++ P T E+ P P +SP
Sbjct: 729 VAKSPPPPSPVYYPPVTQSPPPPSTPVEYHPPASPNQSP 767
>At3g51340.1 68416.m05620 aspartyl protease family protein contains
Eukaryotic and viral aspartyl proteases active site,
PROSITE:PS00141
Length = 518
Score = 28.7 bits (61), Expect = 2.7
Identities = 17/75 (22%), Positives = 32/75 (42%), Gaps = 1/75 (1%)
Frame = +2
Query: 188 RVCQEKENLLGINWQPGN-YPKSELSRTSPKGIEDATPKPAGAYRPPGARNKPSNFTLHE 364
R+ ++E ++ + W+ N + L+ SP E P P+ + PP A P
Sbjct: 430 RIVFDRERMI-LGWKQSNCFEDESLASESPPPPEIEAPPPSVSTPPPAASATPPTIDPRN 488
Query: 365 HEKPHKPGESPNVAP 409
+ G + N++P
Sbjct: 489 STRNSGTGGAANLSP 503
>At5g44340.1 68418.m05429 tubulin beta-4 chain (TUB4) nearly
identical to SP|P24636 Tubulin beta-4 chain {Arabidopsis
thaliana}
Length = 444
Score = 28.3 bits (60), Expect = 3.5
Identities = 24/96 (25%), Positives = 39/96 (40%), Gaps = 6/96 (6%)
Frame = +2
Query: 26 TRGYKKLGACSAPDTTCLQWDPRGETFFTATTYPRLTVGNGYKIWHYTGVLMHKRV---- 193
+RG ++ A S P+ T WD + + R + + G L K V
Sbjct: 275 SRGSQQYSALSVPELTQQMWDAKNMMCAADPRHGRYLTASAV----FRGKLSTKEVDEQM 330
Query: 194 --CQEKENLLGINWQPGNYPKSELSRTSPKGIEDAT 295
Q K + + W P N KS + +PKG++ A+
Sbjct: 331 MNIQNKNSSYFVEWIPNNV-KSSVCDIAPKGLKMAS 365
>At4g31160.1 68417.m04423 transducin family protein / WD-40 repeat
family protein contains 2 WD-40 repeats (PF00400);
KIAA0800 protein, Homo sapiens GI:3882321 EMBL:AB018343
Length = 1846
Score = 28.3 bits (60), Expect = 3.5
Identities = 10/32 (31%), Positives = 19/32 (59%)
Frame = +2
Query: 14 EVWDTRGYKKLGACSAPDTTCLQWDPRGETFF 109
E+WD R +K L + + D T + ++ RG+ +
Sbjct: 1618 EIWDMRTFKLLRSVPSLDQTAITFNSRGDVIY 1649
>At4g38560.1 68417.m05459 expressed protein
Length = 521
Score = 27.9 bits (59), Expect = 4.7
Identities = 13/35 (37%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +2
Query: 233 PGNYPKSELSRTSPKGIEDATPKPAGAY-RPPGAR 334
P P+ RT +G TP+P G+Y PP +R
Sbjct: 158 PDTRPRKSEHRTRNQGPRSKTPEPRGSYLEPPRSR 192
>At3g19020.1 68416.m02415 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 956
Score = 27.9 bits (59), Expect = 4.7
Identities = 22/58 (37%), Positives = 26/58 (44%)
Frame = +2
Query: 245 PKSELSRTSPKGIEDATPKPAGAYRPPGARNKPSNFTLHEHEKPHKPGESPNVAPSKQ 418
PK E S+ P E++ PKP PP P + E P KP ESP P KQ
Sbjct: 507 PKQESSKQEPPKPEES-PKP----EPPKPEESPKPQP-PKQETP-KPEESPKPQPPKQ 557
>At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein
similar to SP|Q01205 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Rattus norvegicus}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 464
Score = 27.5 bits (58), Expect = 6.2
Identities = 19/49 (38%), Positives = 22/49 (44%)
Frame = +2
Query: 284 EDATPKPAGAYRPPGARNKPSNFTLHEHEKPHKPGESPNVAPSKQAIKQ 430
E A KPA PP KP + EKP P P PSKQ+ K+
Sbjct: 181 EKAPEKPAPKPSPPA--EKPKVESTKVAEKPKAPSPPP-PPPSKQSAKE 226
>At5g43020.1 68418.m05248 leucine-rich repeat transmembrane protein
kinase, putative
Length = 669
Score = 27.5 bits (58), Expect = 6.2
Identities = 15/29 (51%), Positives = 18/29 (62%)
Frame = +2
Query: 266 TSPKGIEDATPKPAGAYRPPGARNKPSNF 352
TS G EDA A AY+PP AR+K N+
Sbjct: 533 TSNDGQEDAD---AAAYKPPEARHKSLNY 558
>At5g32590.1 68418.m03867 myosin heavy chain-related similar to
Myosin heavy chain, non-muscle (Zipper protein) (Myosin
II)(SP:Q99323) {Drosophila melanogaster}
Length = 761
Score = 27.5 bits (58), Expect = 6.2
Identities = 18/51 (35%), Positives = 20/51 (39%), Gaps = 1/51 (1%)
Frame = +2
Query: 248 KSELSRTSPKGIEDAT-PKPAGAYRPPGARNKPSNFTLHEHEKPHKPGESP 397
K E SRT A + AGAYRP R S H PH+ P
Sbjct: 299 KKEYSRTLLVDDGSAEGARSAGAYRPTPHREGHSGRLSHRERSPHRDSPRP 349
>At1g20130.1 68414.m02518 family II extracellular lipase, putative
contains Pfam profile PF00657: GDSL-like
Lipase/Acylhydrolase; similar to EXL3 (PMID:11431566)
Length = 1006
Score = 27.5 bits (58), Expect = 6.2
Identities = 16/47 (34%), Positives = 19/47 (40%)
Frame = +2
Query: 272 PKGIEDATPKPAGAYRPPGARNKPSNFTLHEHEKPHKPGESPNVAPS 412
PK PKP PP P+ + H P KP +P APS
Sbjct: 85 PKPAPPPAPKPVPCPSPPKPP-APTPKPVPPHGPPPKPAPAPTPAPS 130
>At5g53870.1 68418.m06701 plastocyanin-like domain-containing
protein contains similarity to SP|Q02917 Early nodulin
55-2 precursor {Glycine max}; PF02298: Plastocyanin-like
domain
Length = 370
Score = 27.1 bits (57), Expect = 8.2
Identities = 16/43 (37%), Positives = 19/43 (44%), Gaps = 2/43 (4%)
Frame = +2
Query: 290 ATPKPAGAYRPPGARNKPSNFTLH--EHEKPHKPGESPNVAPS 412
ATP P+ P + PS+ H H H P SP APS
Sbjct: 205 ATPSPSPK-SPSPVSHSPSHSPAHTPSHSPAHTPSHSPAHAPS 246
>At5g04560.1 68418.m00456 DEMETER protein (DME) identical to DEMETER
protein [Arabidopsis thaliana] GI:21743571; contains
Pfam profile PF00730: HhH-GPD superfamily base excision
DNA repair protein
Length = 1729
Score = 27.1 bits (57), Expect = 8.2
Identities = 14/33 (42%), Positives = 19/33 (57%)
Frame = +2
Query: 227 WQPGNYPKSELSRTSPKGIEDATPKPAGAYRPP 325
WQP PK+ LSR+ PKG + + +G R P
Sbjct: 610 WQP-TPPKTPLSRSKPKGKGRKSIQDSGKARGP 641
>At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest
subunit (RPB205) (RPII) (RPB1) nearly identical to
P|P18616 DNA-directed RNA polymerase II largest subunit
(EC 2.7.7.6) {Arabidopsis thaliana}
Length = 1840
Score = 27.1 bits (57), Expect = 8.2
Identities = 19/64 (29%), Positives = 27/64 (42%)
Frame = +2
Query: 221 INWQPGNYPKSELSRTSPKGIEDATPKPAGAYRPPGARNKPSNFTLHEHEKPHKPGESPN 400
I + P N S S SP + P+ Y P PS+ T + P+ G SP+
Sbjct: 1738 IAYSPSNARLSPASPYSPTSPNYSPTSPS--YSPTSPSYSPSSPT-YSPSSPYSSGASPD 1794
Query: 401 VAPS 412
+PS
Sbjct: 1795 YSPS 1798
>At4g10750.1 68417.m01755 HpcH/HpaI aldolase family protein similar
to 2,4-dihydroxydec-2-ene-1,10-dioic acid aldolase
[Sphingopyxis macrogoltabida] GI:23330203; contains Pfam
profile PF03328: HpcH/HpaI aldolase family
Length = 358
Score = 27.1 bits (57), Expect = 8.2
Identities = 11/34 (32%), Positives = 16/34 (47%)
Frame = +2
Query: 215 LGINWQPGNYPKSELSRTSPKGIEDATPKPAGAY 316
LG W PG+ E+ + + K + P GAY
Sbjct: 255 LGYLWDPGHKKVREMMKKAEKSVLTTDPAKGGAY 288
>At1g28290.1 68414.m03472 pollen Ole e 1 allergen and extensin
family protein similar to arabinogalactan protein
[Daucus carota] GI:11322245; contains Pfam profile
PF01190: Pollen proteins Ole e I family
Length = 359
Score = 27.1 bits (57), Expect = 8.2
Identities = 13/43 (30%), Positives = 18/43 (41%)
Frame = +2
Query: 296 PKPAGAYRPPGARNKPSNFTLHEHEKPHKPGESPNVAPSKQAI 424
P PA + + P + H H PH P +SP P K +
Sbjct: 38 PAPAPYHHGHHHPHPPHHHHPHPHPHPHPPAKSPVKPPVKAPV 80
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,489,874
Number of Sequences: 28952
Number of extensions: 297881
Number of successful extensions: 972
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 919
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 967
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1023490624
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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