Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0006_M16
(378 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g13890.1 68417.m02152 glycine hydroxymethyltransferase, putat... 130 3e-31
At4g37930.1 68417.m05363 glycine hydroxymethyltransferase / seri... 122 1e-28
At4g13930.1 68417.m02156 glycine hydroxymethyltransferase, putat... 121 2e-28
At4g32520.1 68417.m04629 glycine hydroxymethyltransferase, putat... 120 2e-28
At5g26780.3 68418.m03195 glycine hydroxymethyltransferase, putat... 116 5e-27
At5g26780.2 68418.m03194 glycine hydroxymethyltransferase, putat... 116 5e-27
At5g26780.1 68418.m03193 glycine hydroxymethyltransferase, putat... 116 5e-27
At1g22020.1 68414.m02755 glycine hydroxymethyltransferase, putat... 111 1e-25
At1g36370.1 68414.m04518 glycine hydroxymethyltransferase, putat... 99 5e-22
At5g64760.1 68418.m08143 26S proteasome regulatory subunit, puta... 31 0.25
At5g67370.1 68418.m08495 expressed protein similar to unknown pr... 28 2.4
At5g13380.1 68418.m01541 auxin-responsive GH3 family protein sim... 27 4.1
At4g24880.1 68417.m03560 expressed protein 27 4.1
At3g24170.1 68416.m03034 glutathione reductase, putative identic... 27 5.5
At5g54100.1 68418.m06736 band 7 family protein similar to stomat... 26 7.2
At1g74120.1 68414.m08584 mitochondrial transcription termination... 26 7.2
At1g08210.1 68414.m00907 aspartyl protease family protein contai... 26 7.2
At5g16730.1 68418.m01959 expressed protein weak similarity to mi... 26 9.6
>At4g13890.1 68417.m02152 glycine hydroxymethyltransferase, putative
/ serine hydroxymethyltransferase, putative /
serine/threonine aldolase, putative similar to serine
hydroxymethyltransferase [Chlamydomonas reinhardtii]
GI:17066746; contains Pfam profile PF00464: serine
hydroxymethyltransferase
Length = 470
Score = 130 bits (314), Expect = 3e-31
Identities = 60/93 (64%), Positives = 73/93 (78%)
Frame = +1
Query: 100 NGNLWETDPELFNLITKEKKRQQAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPYQRY 279
N +L DPE+++LI KEK RQ G+E+IA+ENFTSV V++ L SCL NKYSEGMP RY
Sbjct: 9 NTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYSEGMPGNRY 68
Query: 280 YGGNEFIDEIEILAQNRSLETYKLNPEEWGVNV 378
YGG EFIDEIE L ++RSLE + NPE+WGVNV
Sbjct: 69 YGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNV 101
>At4g37930.1 68417.m05363 glycine hydroxymethyltransferase / serine
hydroxymethyltransferase / serine/threonine aldolase
(SHM1) identical to serine hydroxymethyl transferase
[Arabidopsis thaliana] GI:6899945
Length = 517
Score = 122 bits (293), Expect = 1e-28
Identities = 57/96 (59%), Positives = 70/96 (72%)
Frame = +1
Query: 91 KLLNGNLWETDPELFNLITKEKKRQQAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPY 270
K LN L E DPE+ ++I EK RQ GLE+I SENFTSV V+Q + S + NKYSEG P
Sbjct: 49 KQLNAPLEEVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPG 108
Query: 271 QRYYGGNEFIDEIEILAQNRSLETYKLNPEEWGVNV 378
RYYGGNE+ID E L Q R+LE ++L+PE+WGVNV
Sbjct: 109 ARYYGGNEYIDMAETLCQKRALEAFRLDPEKWGVNV 144
>At4g13930.1 68417.m02156 glycine hydroxymethyltransferase, putative
/ serine hydroxymethyltransferase, putative /
serine/threonine aldolase, putative similar to serine
hydroxymethyltransferase [Chlamydomonas reinhardtii]
GI:17066746; contains Pfam profile PF00464: serine
hydroxymethyltransferase
Length = 471
Score = 121 bits (291), Expect = 2e-28
Identities = 56/93 (60%), Positives = 70/93 (75%)
Frame = +1
Query: 100 NGNLWETDPELFNLITKEKKRQQAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPYQRY 279
N +L DPE+ +LI KEK+RQ G+E+IASENFTS V++ L S L NKYSEG+P RY
Sbjct: 9 NTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGIPGNRY 68
Query: 280 YGGNEFIDEIEILAQNRSLETYKLNPEEWGVNV 378
YGGNEFIDEIE L ++R+LE + +P WGVNV
Sbjct: 69 YGGNEFIDEIENLCRSRALEAFHCDPAAWGVNV 101
>At4g32520.1 68417.m04629 glycine hydroxymethyltransferase, putative
/ serine hydroxymethyltransferase, putative /
serine/threonine aldolase, putative similar to serine
hydroxymethyltransferase [Chlamydomonas reinhardtii]
GI:17066746; contains Pfam profile PF00464: serine
hydroxymethyltransferase
Length = 529
Score = 120 bits (290), Expect = 2e-28
Identities = 54/90 (60%), Positives = 70/90 (77%)
Frame = +1
Query: 109 LWETDPELFNLITKEKKRQQAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPYQRYYGG 288
L E DPE+ +ITKEK RQ LE+IASENFTS V++ + SCL NKYSEG+P +RYYGG
Sbjct: 83 LGEVDPEVRTIITKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGG 142
Query: 289 NEFIDEIEILAQNRSLETYKLNPEEWGVNV 378
NE+ID++E L QNR+L ++L+ +WGVNV
Sbjct: 143 NEYIDQLETLCQNRALAAFRLDSTKWGVNV 172
>At5g26780.3 68418.m03195 glycine hydroxymethyltransferase, putative
/ serine hydroxymethyltransferase, putative /
serine/threonine aldolase, putative strong similarity to
SP|P50433 Serine hydroxymethyltransferase, mitochondrial
precursor (EC 2.1.2.1) (Serine methylase) (Glycine
hydroxymethyltransferase) (SHMT) {Solanum tuberosum};
contains Pfam profile PF00464: serine
hydroxymethyltransferase
Length = 533
Score = 116 bits (279), Expect = 5e-27
Identities = 54/96 (56%), Positives = 69/96 (71%)
Frame = +1
Query: 91 KLLNGNLWETDPELFNLITKEKKRQQAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPY 270
K LN +L E DPE+ ++I EK RQ G E+I SENFTS+ V+Q + S + NKYSEG P
Sbjct: 49 KQLNASLDEIDPEVADIIELEKARQWKGFELIPSENFTSLSVMQAVGSVMTNKYSEGYPG 108
Query: 271 QRYYGGNEFIDEIEILAQNRSLETYKLNPEEWGVNV 378
RYYGGNE+ID E L Q R+LE ++L+P +WGVNV
Sbjct: 109 ARYYGGNEYIDMAETLCQKRALEAFQLDPSKWGVNV 144
>At5g26780.2 68418.m03194 glycine hydroxymethyltransferase, putative
/ serine hydroxymethyltransferase, putative /
serine/threonine aldolase, putative strong similarity to
SP|P50433 Serine hydroxymethyltransferase, mitochondrial
precursor (EC 2.1.2.1) (Serine methylase) (Glycine
hydroxymethyltransferase) (SHMT) {Solanum tuberosum};
contains Pfam profile PF00464: serine
hydroxymethyltransferase
Length = 533
Score = 116 bits (279), Expect = 5e-27
Identities = 54/96 (56%), Positives = 69/96 (71%)
Frame = +1
Query: 91 KLLNGNLWETDPELFNLITKEKKRQQAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPY 270
K LN +L E DPE+ ++I EK RQ G E+I SENFTS+ V+Q + S + NKYSEG P
Sbjct: 49 KQLNASLDEIDPEVADIIELEKARQWKGFELIPSENFTSLSVMQAVGSVMTNKYSEGYPG 108
Query: 271 QRYYGGNEFIDEIEILAQNRSLETYKLNPEEWGVNV 378
RYYGGNE+ID E L Q R+LE ++L+P +WGVNV
Sbjct: 109 ARYYGGNEYIDMAETLCQKRALEAFQLDPSKWGVNV 144
>At5g26780.1 68418.m03193 glycine hydroxymethyltransferase, putative
/ serine hydroxymethyltransferase, putative /
serine/threonine aldolase, putative strong similarity to
SP|P50433 Serine hydroxymethyltransferase, mitochondrial
precursor (EC 2.1.2.1) (Serine methylase) (Glycine
hydroxymethyltransferase) (SHMT) {Solanum tuberosum};
contains Pfam profile PF00464: serine
hydroxymethyltransferase
Length = 517
Score = 116 bits (279), Expect = 5e-27
Identities = 54/96 (56%), Positives = 69/96 (71%)
Frame = +1
Query: 91 KLLNGNLWETDPELFNLITKEKKRQQAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPY 270
K LN +L E DPE+ ++I EK RQ G E+I SENFTS+ V+Q + S + NKYSEG P
Sbjct: 49 KQLNASLDEIDPEVADIIELEKARQWKGFELIPSENFTSLSVMQAVGSVMTNKYSEGYPG 108
Query: 271 QRYYGGNEFIDEIEILAQNRSLETYKLNPEEWGVNV 378
RYYGGNE+ID E L Q R+LE ++L+P +WGVNV
Sbjct: 109 ARYYGGNEYIDMAETLCQKRALEAFQLDPSKWGVNV 144
>At1g22020.1 68414.m02755 glycine hydroxymethyltransferase, putative
/ serine hydroxymethyltransferase, putative /
serine/threonine aldolase, putative similar to serine
hydroxymethyltransferase [Chlamydomonas reinhardtii]
GI:17066746; contains Pfam profile PF00464: serine
hydroxymethyltransferase
Length = 599
Score = 111 bits (268), Expect = 1e-25
Identities = 52/93 (55%), Positives = 67/93 (72%)
Frame = +1
Query: 100 NGNLWETDPELFNLITKEKKRQQAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPYQRY 279
N ++ E DPE+ + KEK+RQ G+E+IASENF V++ L S L NKYSEGMP RY
Sbjct: 139 NQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARY 198
Query: 280 YGGNEFIDEIEILAQNRSLETYKLNPEEWGVNV 378
Y GN++ID+IEIL Q R+L + LN E+WGVNV
Sbjct: 199 YTGNQYIDQIEILCQERALAAFGLNHEKWGVNV 231
>At1g36370.1 68414.m04518 glycine hydroxymethyltransferase, putative
/ serine hydroxymethyltransferase, putative /
serine/threonine aldolase, putative similar to serine
hydroxymethyltransferase [Chlamydomonas reinhardtii]
GI:17066746; contains Pfam profile PF00464: serine
hydroxymethyltransferase
Length = 598
Score = 99 bits (238), Expect = 5e-22
Identities = 46/86 (53%), Positives = 61/86 (70%)
Frame = +1
Query: 121 DPELFNLITKEKKRQQAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPYQRYYGGNEFI 300
DP++ L+ KEK+RQ G+E+IASENF V++ L S L NKYSEGMP RYY GN++I
Sbjct: 142 DPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQYI 201
Query: 301 DEIEILAQNRSLETYKLNPEEWGVNV 378
D+IE L R+L + L ++WGVNV
Sbjct: 202 DQIENLCIERALTAFGLESDKWGVNV 227
>At5g64760.1 68418.m08143 26S proteasome regulatory subunit,
putative (RPN5)
Length = 442
Score = 31.1 bits (67), Expect = 0.25
Identities = 27/84 (32%), Positives = 39/84 (46%), Gaps = 2/84 (2%)
Frame = +1
Query: 121 DPELFNL-ITKEKKRQQAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPYQRYYG-GNE 294
+P +F+ TKEKK+ + G M+ E +P L L Y E M RYY NE
Sbjct: 177 NPRVFDADTTKEKKKPKEGENMV-EEAPADIPTLLVLKRI----YYELMI--RYYSHNNE 229
Query: 295 FIDEIEILAQNRSLETYKLNPEEW 366
+I+ + + K NPE+W
Sbjct: 230 YIEICRSYKAIYDIPSVKENPEQW 253
>At5g67370.1 68418.m08495 expressed protein similar to unknown
protein (gb|AAC18972.1)
Length = 327
Score = 27.9 bits (59), Expect = 2.4
Identities = 12/34 (35%), Positives = 22/34 (64%), Gaps = 2/34 (5%)
Frame = +1
Query: 262 MPYQR--YYGGNEFIDEIEILAQNRSLETYKLNP 357
+PY+ +Y G ++ E+LA++R L +YK+ P
Sbjct: 183 IPYEESGWYDGQMWVKPPEVLARDRLLGSYKVKP 216
>At5g13380.1 68418.m01541 auxin-responsive GH3 family protein
similar to auxin-responsive GH3 product [Glycine max]
GI:18591; contains Pfam profile PF03321: GH3
auxin-responsive promoter
Length = 624
Score = 27.1 bits (57), Expect = 4.1
Identities = 19/63 (30%), Positives = 32/63 (50%), Gaps = 1/63 (1%)
Frame = +1
Query: 103 GN-LWETDPELFNLITKEKKRQQAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPYQRY 279
GN L DP+L NLI E++ + E I S + P +C+ + + ++ +P +
Sbjct: 290 GNFLTAPDPDLANLI--EQECSKTSWEAILSRIW---PKAKCIEAVITGTMAQYIPLLEF 344
Query: 280 YGG 288
YGG
Sbjct: 345 YGG 347
>At4g24880.1 68417.m03560 expressed protein
Length = 417
Score = 27.1 bits (57), Expect = 4.1
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = +1
Query: 199 FTSVPVLQCLSSCLHNKYSEGMPYQR 276
F+ VP C S LH+ Y+ +PY R
Sbjct: 230 FSVVPFYNCDQSGLHSAYTGSLPYVR 255
>At3g24170.1 68416.m03034 glutathione reductase, putative identical
to GB:P48641 from [Arabidopsis thaliana]
Length = 499
Score = 26.6 bits (56), Expect = 5.5
Identities = 12/31 (38%), Positives = 18/31 (58%)
Frame = +1
Query: 286 GNEFIDEIEILAQNRSLETYKLNPEEWGVNV 378
G EF+ ++ + A RS T +LN E GV +
Sbjct: 284 GEEFVADVVLFATGRSPNTKRLNLEAVGVEL 314
>At5g54100.1 68418.m06736 band 7 family protein similar to
stomatin-like protein [Zea mays] GI:7716464; contains
Pfam profile PF01145: SPFH domain / Band 7 family
Length = 401
Score = 26.2 bits (55), Expect = 7.2
Identities = 14/51 (27%), Positives = 22/51 (43%), Gaps = 1/51 (1%)
Frame = +1
Query: 229 SSCLHNKYSEGMPYQRYYGGNEFIDEIEILAQNRSLETYKLN-PEEWGVNV 378
S LH S G P Q+ +G F + + +Y +N P WG+ +
Sbjct: 64 SRLLHQSSSAGTPPQQLFGARSF------SSPSSDFNSYHINPPSNWGIRI 108
>At1g74120.1 68414.m08584 mitochondrial transcription termination
factor-related / mTERF-related contains Pfam profile
PF02536: mTERF
Length = 445
Score = 26.2 bits (55), Expect = 7.2
Identities = 15/48 (31%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
Frame = +1
Query: 7 YRKKYIFFLYFQ-FSIPVRNFNYTSTMNNKLLNGNLWETDPELFNLIT 147
YRK+ + Q + P + + + NN LLN +L ET+ L L++
Sbjct: 38 YRKRILLANLLQRYGFPPSSLQHFLSRNNHLLNSDLVETEISLGILLS 85
>At1g08210.1 68414.m00907 aspartyl protease family protein contains
Pfam profile PF00026: Eukaryotic aspartyl protease;
similar to CND41, chloroplast nucleoid DNA binding
protein (GI:2541876) {Nicotiana tabacum}
Length = 492
Score = 26.2 bits (55), Expect = 7.2
Identities = 13/71 (18%), Positives = 32/71 (45%)
Frame = +1
Query: 67 NYTSTMNNKLLNGNLWETDPELFNLITKEKKRQQAGLEMIASENFTSVPVLQCLSSCLHN 246
+Y + + +NG + DP +F + T + G + + P +Q +++ + +
Sbjct: 285 HYNVNLQSIAVNGQILPIDPSVFTIATGDGTIIDTGTTLAYLPDEAYSPFIQAVANAV-S 343
Query: 247 KYSEGMPYQRY 279
+Y + Y+ Y
Sbjct: 344 QYGRPITYESY 354
>At5g16730.1 68418.m01959 expressed protein weak similarity to
microtubule binding protein D-CLIP-190 [Drosophila
melanogaster] GI:2773363, SMC2-like condensin
[Arabidopsis thaliana] GI:14279543
Length = 853
Score = 25.8 bits (54), Expect = 9.6
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = +1
Query: 142 ITKEKKRQQAGLEMIASENFTSVPVLQCLSSCLHNKYSEG 261
+++EK + + LE E S ++ L+S LH SEG
Sbjct: 441 LSEEKSKLLSDLESSKEEEEKSKKAMESLASALHEVSSEG 480
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,250,773
Number of Sequences: 28952
Number of extensions: 160892
Number of successful extensions: 384
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 376
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 384
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 517767328
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -