Samia BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0006_M15
         (569 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g05980.1 68418.m00662 dihydrofolate synthetase/folylpolygluta...    96   1e-20
At3g55630.3 68416.m06181 dihydrofolate synthetase/folylpolygluta...    86   2e-17
At3g55630.2 68416.m06180 dihydrofolate synthetase/folylpolygluta...    86   2e-17
At3g55630.1 68416.m06179 dihydrofolate synthetase/folylpolygluta...    86   2e-17
At5g41480.1 68418.m05037 dihydrofolate synthetase/folylpolygluta...    68   4e-12
At3g10160.1 68416.m01218 dihydrofolate synthetase/folylpolygluta...    46   1e-05
At1g63680.1 68414.m07206 Mur ligase family protein contains Pfam...    33   0.10 
At2g02560.1 68415.m00195 TIP120 protein, putative similar to TBP...    32   0.31 
At1g34630.1 68414.m04305 expressed protein                             32   0.31 
At5g56360.1 68418.m07034 calmodulin-binding protein similar to a...    30   0.95 
At2g28050.1 68415.m03401 pentatricopeptide (PPR) repeat-containi...    29   1.7  
At3g21480.1 68416.m02710 transcription activation domain-interac...    28   3.8  
At5g62350.1 68418.m07826 invertase/pectin methylesterase inhibit...    27   6.7  
At1g28020.1 68414.m03431 pentatricopeptide (PPR) repeat-containi...    27   6.7  
At3g04350.1 68416.m00460 expressed protein                             27   8.8  

>At5g05980.1 68418.m00662 dihydrofolate
           synthetase/folylpolyglutamate synthetase (DHFS/FPGS2)
           nearly identical to gi:17976705; identical to cDNA
           dihydrofolate synthetase/folylpolyglutamate synthetase
           (dhfs/fpgs2 gene) GI:17976704
          Length = 571

 Score = 96.3 bits (229), Expect = 1e-20
 Identities = 58/135 (42%), Positives = 77/135 (57%)
 Frame = +3

Query: 165 NYEDAVHKLNKLQSNKSTIDQIRKDLRTGKRCTNLEDMERYLFRTGVTLTKLDELSVIHV 344
           +YE+A+  L+ L + +S  D+  K    G R   + D  + L      L    +++VIHV
Sbjct: 66  SYEEALAALSSLITKRSRADKSNK----GDRFELVFDYLKLLDLEEDIL----KMNVIHV 117

Query: 345 AGTKGKGSTSAMCDSILRHHGFRTGFFSSPHLIAVRERIRLDGKIVSEEKFAQHFHRVYN 524
           AGTKGKGST    +SI+R++GFRTG F+SPHLI VRER RLDG  +SEEKF  +F   YN
Sbjct: 118 AGTKGKGSTCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYN 177

Query: 525 XXXXXXXXXXXXPKY 569
                       P Y
Sbjct: 178 RLKERTNEEIPMPTY 192


>At3g55630.3 68416.m06181 dihydrofolate
           synthetase/folylpolyglutamate synthetase (DHFS/FPGS4)
           nearly identical to folylpolyglutamate-dihydrofolate
           synthetase [Arabidopsis thaliana] GI:17976761
          Length = 492

 Score = 85.8 bits (203), Expect = 2e-17
 Identities = 38/64 (59%), Positives = 49/64 (76%)
 Frame = +3

Query: 318 LDELSVIHVAGTKGKGSTSAMCDSILRHHGFRTGFFSSPHLIAVRERIRLDGKIVSEEKF 497
           + ++ +IHVAGTKGKGST    +SILR +G RTG F+SPHLI VRER RL+G  +S+EKF
Sbjct: 55  VSQMKIIHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKF 114

Query: 498 AQHF 509
             +F
Sbjct: 115 VNYF 118


>At3g55630.2 68416.m06180 dihydrofolate
           synthetase/folylpolyglutamate synthetase (DHFS/FPGS4)
           nearly identical to folylpolyglutamate-dihydrofolate
           synthetase [Arabidopsis thaliana] GI:17976761
          Length = 491

 Score = 85.8 bits (203), Expect = 2e-17
 Identities = 38/64 (59%), Positives = 49/64 (76%)
 Frame = +3

Query: 318 LDELSVIHVAGTKGKGSTSAMCDSILRHHGFRTGFFSSPHLIAVRERIRLDGKIVSEEKF 497
           + ++ +IHVAGTKGKGST    +SILR +G RTG F+SPHLI VRER RL+G  +S+EKF
Sbjct: 55  VSQMKIIHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKF 114

Query: 498 AQHF 509
             +F
Sbjct: 115 VNYF 118


>At3g55630.1 68416.m06179 dihydrofolate
           synthetase/folylpolyglutamate synthetase (DHFS/FPGS4)
           nearly identical to folylpolyglutamate-dihydrofolate
           synthetase [Arabidopsis thaliana] GI:17976761
          Length = 470

 Score = 85.8 bits (203), Expect = 2e-17
 Identities = 38/64 (59%), Positives = 49/64 (76%)
 Frame = +3

Query: 318 LDELSVIHVAGTKGKGSTSAMCDSILRHHGFRTGFFSSPHLIAVRERIRLDGKIVSEEKF 497
           + ++ +IHVAGTKGKGST    +SILR +G RTG F+SPHLI VRER RL+G  +S+EKF
Sbjct: 55  VSQMKIIHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKF 114

Query: 498 AQHF 509
             +F
Sbjct: 115 VNYF 118


>At5g41480.1 68418.m05037 dihydrofolate
           synthetase/folylpolyglutamate synthetase (DHFS/FPGS1)
           nearly identical to GI:17976703; identical to cDNA
           dihydrofolate synthetase/folylpolyglutamate synthetase
           (dhfs/fpgs1 gene) GI:17976702
          Length = 530

 Score = 68.1 bits (159), Expect = 4e-12
 Identities = 31/62 (50%), Positives = 43/62 (69%)
 Frame = +3

Query: 333 VIHVAGTKGKGSTSAMCDSILRHHGFRTGFFSSPHLIAVRERIRLDGKIVSEEKFAQHFH 512
           V+HVAGTKGKGSTSA   +ILR  G+  G +SSPH+++++ERI  +G+ VS       F+
Sbjct: 96  VVHVAGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISCNGEPVSASTLNDLFY 155

Query: 513 RV 518
            V
Sbjct: 156 SV 157


>At3g10160.1 68416.m01218 dihydrofolate
           synthetase/folylpolyglutamate synthetase (DHFS/FPGS3)
           nearly identical to gi:17976757
          Length = 464

 Score = 46.4 bits (105), Expect = 1e-05
 Identities = 20/29 (68%), Positives = 24/29 (82%)
 Frame = +3

Query: 423 FSSPHLIAVRERIRLDGKIVSEEKFAQHF 509
           F+SPHLI VRER R+DG  +SEEKF Q+F
Sbjct: 2   FTSPHLIDVRERFRIDGLDISEEKFLQYF 30


>At1g63680.1 68414.m07206 Mur ligase family protein contains Pfam
           profile: PF01225 Mur ligase family, catalytic domain
          Length = 767

 Score = 33.5 bits (73), Expect = 0.10
 Identities = 16/35 (45%), Positives = 22/35 (62%)
 Frame = +3

Query: 327 LSVIHVAGTKGKGSTSAMCDSILRHHGFRTGFFSS 431
           +SVI V GT GK +T+ +  S+    G RTG FS+
Sbjct: 344 MSVIGVTGTDGKTTTTYLIKSLYEAMGVRTGMFST 378


>At2g02560.1 68415.m00195 TIP120 protein, putative similar to
            TBP-interacting protein TIP120 GI:1799570 from [Rattus
            norvegicus]
          Length = 1219

 Score = 31.9 bits (69), Expect = 0.31
 Identities = 24/69 (34%), Positives = 36/69 (52%), Gaps = 1/69 (1%)
 Frame = -2

Query: 499  ANFSSETILPSRRILSLTAIKCGDEKNPVRNP*CRSIESHIALVEPFPLVPATCI-TDSS 323
            A+F + ++    +IL+L    C  E+  VRN     +   +AL+EP  LVPA  + T S 
Sbjct: 922  ADFQNSSV---EKILALLFNHCESEEEGVRNVVAECL-GKMALIEPEKLVPALQVRTTSP 977

Query: 322  SNFVRVTPV 296
            + F R T V
Sbjct: 978  AAFTRATVV 986


>At1g34630.1 68414.m04305 expressed protein
          Length = 481

 Score = 31.9 bits (69), Expect = 0.31
 Identities = 27/77 (35%), Positives = 39/77 (50%)
 Frame = +3

Query: 93  LMCSSSLYRSYLVNKLVRMYSVKSNYEDAVHKLNKLQSNKSTIDQIRKDLRTGKRCTNLE 272
           LMC SS     L   +++  S+ S+Y+     LNK      +I Q  KD+ T +  TNL 
Sbjct: 210 LMCLSS--SQILSAYILKQESLPSSYKSF---LNKQGGKDLSILQGVKDIATAQPFTNLR 264

Query: 273 DMERYLFRTGVTLTKLD 323
            +E+Y    GV + KLD
Sbjct: 265 AIEKYYKSVGVDI-KLD 280


>At5g56360.1 68418.m07034 calmodulin-binding protein similar to
           alpha glucosidase II beta subunit from GI:2104691 [Mus
           musculus]
          Length = 647

 Score = 30.3 bits (65), Expect = 0.95
 Identities = 17/62 (27%), Positives = 35/62 (56%)
 Frame = +3

Query: 42  TYCNTTSFEIV*STFDRLMCSSSLYRSYLVNKLVRMYSVKSNYEDAVHKLNKLQSNKSTI 221
           T  N T  E +  T   ++ + +L+++  V+K      V+  Y+++  KLNK+QS  S++
Sbjct: 450 TTSNPTWLEKIQKTVKNILLAVNLFQTTPVDKS-EADRVRKEYDESSSKLNKIQSRISSL 508

Query: 222 DQ 227
           ++
Sbjct: 509 EK 510


>At2g28050.1 68415.m03401 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat
          Length = 462

 Score = 29.5 bits (63), Expect = 1.7
 Identities = 21/95 (22%), Positives = 41/95 (43%), Gaps = 5/95 (5%)
 Frame = +3

Query: 69  IV*STFDRLMCSSSLYRSYLVNKLVRMYSVKSNYEDAVHKLNKLQSNKSTIDQ--IRKDL 242
           IV S  D   C   +   +  N ++ +YS    + + V     +++N+  ID+      L
Sbjct: 123 IVSSVIDECGCEKKVVGRFF-NSMIMVYSDNGKFSEVVEVFEYMKNNEVKIDEKTCTLHL 181

Query: 243 RTGKRCTNLE---DMERYLFRTGVTLTKLDELSVI 338
              KRC  +E   D    +  +G+ +  +  L+V+
Sbjct: 182 LNLKRCDQMELARDFFSLMVESGIDVVTVYSLTVV 216


>At3g21480.1 68416.m02710 transcription activation
           domain-interacting protein-related contains weak
           similarity to Pax transcription activation domain
           interacting protein PTIP (GI:4336734) [Mus musculus]
          Length = 1045

 Score = 28.3 bits (60), Expect = 3.8
 Identities = 23/83 (27%), Positives = 37/83 (44%), Gaps = 5/83 (6%)
 Frame = +3

Query: 162 SNYEDAVHKLNKLQSNKSTIDQIRKDLRTGKRCTN---LEDMERYLFRTGVTLTKLDELS 332
           S  E++V ++   QS  S   + +KDLRTGK+      +ED     F +      L  LS
Sbjct: 225 SGLENSVGEVGNQQSLTSLFVEEKKDLRTGKKTARKLFVEDFPEEKFHSTDCNVDLGNLS 284

Query: 333 VI--HVAGTKGKGSTSAMCDSIL 395
            I     G + + S   + D ++
Sbjct: 285 YIGSQEPGEESQASALNLVDKLI 307


>At5g62350.1 68418.m07826 invertase/pectin methylesterase inhibitor
           family protein / DC 1.2 homolog (FL5-2I22) similar to
           SP|Q42534 Pectinesterase 2 precursor (EC 3.1.1.11)
           (Pectin methylesterase 2) (PE 2) {Arabidopsis thaliana};
           contains Pfam profile PF04043: Plant invertase/pectin
           methylesterase inhibitor; FL5-2I22 mRNA for DC 1.2
           homolog, partial cds GI:11127598
          Length = 202

 Score = 27.5 bits (58), Expect = 6.7
 Identities = 12/55 (21%), Positives = 30/55 (54%)
 Frame = +3

Query: 129 VNKLVRMYSVKSNYEDAVHKLNKLQSNKSTIDQIRKDLRTGKRCTNLEDMERYLF 293
           V++L RM  +K    +A+     ++    T+D++ K ++  K C + +D +++ +
Sbjct: 89  VSRLTRMKGLKKREVEAIKDC--VEEMNDTVDRLTKSVQELKLCGSAKDQDQFAY 141


>At1g28020.1 68414.m03431 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat
          Length = 612

 Score = 27.5 bits (58), Expect = 6.7
 Identities = 23/110 (20%), Positives = 49/110 (44%), Gaps = 3/110 (2%)
 Frame = +3

Query: 78  STFDRLMCSSSLYRSYLVNKLVRMYSVKSNYEDAVHKLNKLQSNKSTIDQIRKD--LRTG 251
           +TF ++     L R    N ++ +YS   N E     L +++ N    D +  +  L+  
Sbjct: 159 ATFQKMRDLGLLLRPVPYNAMMSLYSALKNREKVEELLLEMKDNDVEADNVTVNNVLKLY 218

Query: 252 KRCTNLEDMERYLFR-TGVTLTKLDELSVIHVAGTKGKGSTSAMCDSILR 398
               ++ +ME++L +  G+   KL+  + + +A    +  +S     +LR
Sbjct: 219 SAVCDVTEMEKFLNKWEGIHGIKLEWHTTLDMAKAYLRARSSGKAMKMLR 268


>At3g04350.1 68416.m00460 expressed protein
          Length = 567

 Score = 27.1 bits (57), Expect = 8.8
 Identities = 12/27 (44%), Positives = 20/27 (74%)
 Frame = -2

Query: 271 SKFVHLLPVLKSFLI*SIVDLFDCSLF 191
           +K ++LLP++  F I +IVDLF  +L+
Sbjct: 521 NKIMNLLPLVVRFSIENIVDLFPIALY 547


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,138,184
Number of Sequences: 28952
Number of extensions: 212551
Number of successful extensions: 545
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 539
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 545
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1102220672
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)