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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0006_M14
         (510 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g35670.1 68418.m04261 calmodulin-binding family protein conta...    31   0.34 
At3g17225.1 68416.m02200 invertase/pectin methylesterase inhibit...    29   2.4  
At3g17230.1 68416.m02201 invertase/pectin methylesterase inhibit...    28   4.2  
At1g77620.1 68414.m09037 expressed protein                             28   4.2  
At5g04370.1 68418.m00429 S-adenosyl-L-methionine:carboxyl methyl...    27   5.5  
At1g34370.2 68414.m04268 zinc finger (C2H2 type) family protein ...    27   5.5  
At1g34370.1 68414.m04267 zinc finger (C2H2 type) family protein ...    27   5.5  
At1g15165.1 68414.m01813 U-box domain-containing protein contain...    27   5.5  
At5g16300.2 68418.m01906 expressed protein                             27   7.3  
At5g16300.1 68418.m01905 expressed protein                             27   7.3  
At1g55770.1 68414.m06385 invertase/pectin methylesterase inhibit...    27   9.7  
At1g13580.1 68414.m01592 longevity-assurance (LAG1) family prote...    27   9.7  

>At5g35670.1 68418.m04261 calmodulin-binding family protein contains
           IQ calmodulin-binding motif, Pfam:PF00612
          Length = 442

 Score = 31.5 bits (68), Expect = 0.34
 Identities = 15/37 (40%), Positives = 22/37 (59%), Gaps = 2/37 (5%)
 Frame = -3

Query: 118 NQGKADDLTITKDHRSTPVSS--FRDDRALIISKVFR 14
           NQ +A +  I K H++TP+S     +D A+II   FR
Sbjct: 135 NQSEATETHIPKKHQTTPISKLFLEEDAAVIIQSAFR 171


>At3g17225.1 68416.m02200 invertase/pectin methylesterase inhibitor
           family protein similar to SP|P83326 Pectinesterase
           inhibitor (Pectin methylesterase inhibitor) (PMEI)
           {Actinidia chinensis}; contains Pfam profile PF04043:
           Plant invertase/pectin methylesterase inhibitor
          Length = 185

 Score = 28.7 bits (61), Expect = 2.4
 Identities = 12/23 (52%), Positives = 14/23 (60%)
 Frame = +2

Query: 392 DPNRKEAFEDCARDYHCSIRIIE 460
           DP  K  FE C RDY  +I+ IE
Sbjct: 100 DPTLKIPFETCVRDYEGAIKAIE 122


>At3g17230.1 68416.m02201 invertase/pectin methylesterase inhibitor
           family protein similar to SP|P83326 Pectinesterase
           inhibitor (Pectin methylesterase inhibitor) (PMEI)
           {Actinidia chinensis}; contains Pfam profile PF04043:
           Plant invertase/pectin methylesterase inhibitor
          Length = 351

 Score = 27.9 bits (59), Expect = 4.2
 Identities = 10/23 (43%), Positives = 15/23 (65%)
 Frame = +2

Query: 392 DPNRKEAFEDCARDYHCSIRIIE 460
           DP  + ++E C RDY  +I+ IE
Sbjct: 100 DPTLRTSYETCVRDYEGAIKAIE 122


>At1g77620.1 68414.m09037 expressed protein
          Length = 1151

 Score = 27.9 bits (59), Expect = 4.2
 Identities = 13/31 (41%), Positives = 14/31 (45%)
 Frame = +2

Query: 353 YWVDAGKPTLPDDDPNRKEAFEDCARDYHCS 445
           YW D  K T P  DP+R E  E     Y  S
Sbjct: 759 YWPDMQKETNPLADPSRAEILEAACYQYETS 789


>At5g04370.1 68418.m00429 S-adenosyl-L-methionine:carboxyl
           methyltransferase family protein similar to
           SAM:salicylic acid carboxyl methyltransferase (SAMT)
           [GI:6002712][Clarkia breweri] and to SAM:benzoic acid
           carboxyl methyltransferase
           (BAMT)[GI:9789277][Antirrhinum majus]
          Length = 396

 Score = 27.5 bits (58), Expect = 5.5
 Identities = 16/40 (40%), Positives = 19/40 (47%)
 Frame = +2

Query: 380 LPDDDPNRKEAFEDCARDYHCSIRIIENHMATFGKDCNSD 499
           LP  DPN KE  E   ++    IR +E H    G  CN D
Sbjct: 288 LPFYDPNEKEIKEMVQKEGSFEIRDLETHGYDLG-HCNQD 326


>At1g34370.2 68414.m04268 zinc finger (C2H2 type) family protein
           contains Pfam domain, PF00096: Zinc finger, C2H2 type
          Length = 499

 Score = 27.5 bits (58), Expect = 5.5
 Identities = 15/39 (38%), Positives = 19/39 (48%)
 Frame = +3

Query: 300 CHTNALEVIADLSTYPGYTGSMRGNQRCLMTTLTEKKPL 416
           CHT    VIADL T+  + G  +    C  TT + K  L
Sbjct: 338 CHTKKFSVIADLKTHEKHCGKNKWLCSC-GTTFSRKDKL 375


>At1g34370.1 68414.m04267 zinc finger (C2H2 type) family protein
           contains Pfam domain, PF00096: Zinc finger, C2H2 type
          Length = 499

 Score = 27.5 bits (58), Expect = 5.5
 Identities = 15/39 (38%), Positives = 19/39 (48%)
 Frame = +3

Query: 300 CHTNALEVIADLSTYPGYTGSMRGNQRCLMTTLTEKKPL 416
           CHT    VIADL T+  + G  +    C  TT + K  L
Sbjct: 338 CHTKKFSVIADLKTHEKHCGKNKWLCSC-GTTFSRKDKL 375


>At1g15165.1 68414.m01813 U-box domain-containing protein contains
           Pfam profile PF04564: U-box domain
          Length = 295

 Score = 27.5 bits (58), Expect = 5.5
 Identities = 16/54 (29%), Positives = 27/54 (50%)
 Frame = +1

Query: 199 FRIFMFIINSIRSARIKSQRILHPVSVLCNRM*FVTQMHWRLLRTFLHIPGILG 360
           FR  +F  N++      +  +L P++ L   + F +Q HW+L     HI G +G
Sbjct: 27  FRTELFFSNAVNLEICVAATVLTPLTPLLG-LYFGSQAHWQLNLFGEHIKGFVG 79


>At5g16300.2 68418.m01906 expressed protein 
          Length = 1034

 Score = 27.1 bits (57), Expect = 7.3
 Identities = 24/90 (26%), Positives = 43/90 (47%), Gaps = 4/90 (4%)
 Frame = +1

Query: 127 FSQNKCYQTL*YCLLFKKNDRDLYFRIFMFIINSIRSARIKSQRILHPVSVLCNRM*FVT 306
           + QNKCY ++    L    D++L F     +  +++     S+ I  P +++  +  F+ 
Sbjct: 562 YVQNKCYDSV--SALLADVDKELEF-----LCAAVKKENKDSEAI--PPAIIIEKSLFMG 612

Query: 307 QMHWRLLRTFLHIPGILGR----CGETNAA 384
           ++ + LL    H+P ILG     C ET  A
Sbjct: 613 RLLFALLNHSKHVPLILGSPRLWCRETMTA 642


>At5g16300.1 68418.m01905 expressed protein 
          Length = 1068

 Score = 27.1 bits (57), Expect = 7.3
 Identities = 24/90 (26%), Positives = 43/90 (47%), Gaps = 4/90 (4%)
 Frame = +1

Query: 127 FSQNKCYQTL*YCLLFKKNDRDLYFRIFMFIINSIRSARIKSQRILHPVSVLCNRM*FVT 306
           + QNKCY ++    L    D++L F     +  +++     S+ I  P +++  +  F+ 
Sbjct: 562 YVQNKCYDSV--SALLADVDKELEF-----LCAAVKKENKDSEAI--PPAIIIEKSLFMG 612

Query: 307 QMHWRLLRTFLHIPGILGR----CGETNAA 384
           ++ + LL    H+P ILG     C ET  A
Sbjct: 613 RLLFALLNHSKHVPLILGSPRLWCRETMTA 642


>At1g55770.1 68414.m06385 invertase/pectin methylesterase inhibitor
           family protein low similarity to SP|Q43111
           Pectinesterase 3 precursor (EC 3.1.1.11) (Pectin
           methylesterase 3) {Phaseolus vulgaris}, SP|P83326
           Pectinesterase inhibitor (Pectin methylesterase
           inhibitor) (PMEI) {Actinidia chinensis}; contains Pfam
           profile PF04043: Plant invertase/pectin methylesterase
           inhibitor
          Length = 175

 Score = 26.6 bits (56), Expect = 9.7
 Identities = 9/38 (23%), Positives = 22/38 (57%)
 Frame = +2

Query: 386 DDDPNRKEAFEDCARDYHCSIRIIENHMATFGKDCNSD 499
           D +P  ++  EDC +DY  ++  +++ ++    D ++D
Sbjct: 87  DLEPAIEDTLEDCQKDYQDAVEQLDDSISAMLADAHTD 124


>At1g13580.1 68414.m01592 longevity-assurance (LAG1) family protein
           similar to Alternaria stem canker resistance protein
           (ASC1) [Lycopersicon esculentum] GI:7688742; contains
           Pfam profile PF03798: Longevity-assurance protein (LAG1)
          Length = 239

 Score = 26.6 bits (56), Expect = 9.7
 Identities = 16/56 (28%), Positives = 26/56 (46%), Gaps = 1/56 (1%)
 Frame = +2

Query: 242 VSNLNESCIRCLCYVTGCDLSHKCTGGYCGPFYISRVY-WVDAGKPTLPDDDPNRK 406
           +    ES  +C+ Y++   L+   T  Y  P++++  Y WV  G  T PD     K
Sbjct: 72  IRKFKESAWKCVYYLSAEILALSVT--YNEPWFMNTKYFWVGPGDQTWPDQQTKLK 125


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,884,477
Number of Sequences: 28952
Number of extensions: 223110
Number of successful extensions: 547
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 530
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 547
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 917929344
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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