Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0006_M07
(500 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g21160.1 68416.m02673 mannosyl-oligosaccharide 1,2-alpha-mann... 125 1e-29
At1g30000.1 68414.m03669 glycoside hydrolase family 47 protein s... 121 3e-28
At1g51590.1 68414.m05808 mannosyl-oligosaccharide 1,2-alpha-mann... 120 4e-28
At1g27520.1 68414.m03355 glycoside hydrolase family 47 protein S... 75 3e-14
At5g43710.1 68418.m05344 glycoside hydrolase family 47 protein s... 61 5e-10
At4g12000.1 68417.m01909 expressed protein 30 0.76
At3g50370.1 68416.m05508 expressed protein 29 1.8
At1g60160.1 68414.m06777 potassium transporter family protein si... 29 2.3
At5g09400.1 68418.m01089 potassium transporter family protein si... 28 4.1
At4g35630.1 68417.m05060 phosphoserine aminotransferase, chlorop... 28 4.1
At5g42910.1 68418.m05231 basic leucine zipper transcription fact... 27 5.4
At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative... 27 7.1
At1g47765.1 68414.m05312 F-box family protein contains F-box dom... 27 7.1
At4g19530.1 68417.m02873 disease resistance protein (TIR-NBS-LRR... 27 9.4
At1g15780.1 68414.m01893 expressed protein 27 9.4
>At3g21160.1 68416.m02673 mannosyl-oligosaccharide
1,2-alpha-mannosidase, putative similar to
mannosyl-oligosaccharide 1,2-alpha-mannosidase [Glycine
max][GI:6552504]
Length = 572
Score = 125 bits (302), Expect = 1e-29
Identities = 56/97 (57%), Positives = 70/97 (72%)
Frame = +3
Query: 3 ETFESYFIMWRLTKQQRYRDWGWEAVQALEKHCRVDGGYTGIANVYHQNPQGDDVQQSFF 182
ET ES F +WRLT + Y++WGW +A EK+ R++ GY G+ +V D+ QSFF
Sbjct: 446 ETVESLFYLWRLTGNKTYQEWGWNIFEAFEKNSRIESGYVGLKDV--NTGVKDNKMQSFF 503
Query: 183 LAETLKYLYLLFSDDTLVPLDEWVFNTEAHPLPIKGQ 293
LAETLKYLYLLFS T++PLDEWVFNTEAHPL IK +
Sbjct: 504 LAETLKYLYLLFSPTTVIPLDEWVFNTEAHPLKIKSR 540
>At1g30000.1 68414.m03669 glycoside hydrolase family 47 protein
similar to GI:5579331 from [Homo sapiens]; contains Pfam
profile PF01532: Glycosyl hydrolase family 47
Length = 624
Score = 121 bits (291), Expect = 3e-28
Identities = 53/96 (55%), Positives = 72/96 (75%), Gaps = 1/96 (1%)
Frame = +3
Query: 3 ETFESYFIMWRLTKQQRYRDWGWEAVQALEKHCRV-DGGYTGIANVYHQNPQGDDVQQSF 179
ET ES F+++R+TK +YRD GW+ +A EK+ +V GGYT + +V P D ++F
Sbjct: 526 ETVESLFVLYRITKDTKYRDQGWQIFEAFEKYTKVKSGGYTSLDDVTEVPPHRRDKMETF 585
Query: 180 FLAETLKYLYLLFSDDTLVPLDEWVFNTEAHPLPIK 287
FL ETLKYLYLLF DD+++PLD++VFNTEAHPLPI+
Sbjct: 586 FLGETLKYLYLLFGDDSVIPLDKFVFNTEAHPLPIR 621
>At1g51590.1 68414.m05808 mannosyl-oligosaccharide
1,2-alpha-mannosidase, putative similar to
mannosyl-oligosaccharide 1,2-alpha-mannosidase [Glycine
max][GI:6552504]
Length = 560
Score = 120 bits (290), Expect = 4e-28
Identities = 55/94 (58%), Positives = 67/94 (71%)
Frame = +3
Query: 3 ETFESYFIMWRLTKQQRYRDWGWEAVQALEKHCRVDGGYTGIANVYHQNPQGDDVQQSFF 182
ET ES F +WRLT + Y++WGW QA EK+ RV+ GY G+ +V D+ QSFF
Sbjct: 445 ETVESLFYLWRLTGNKTYQEWGWNIFQAFEKNSRVESGYVGLKDV--NTGAKDNKMQSFF 502
Query: 183 LAETLKYLYLLFSDDTLVPLDEWVFNTEAHPLPI 284
LAETLKYLYLLFS +++ LDEWVFNTEAHPL I
Sbjct: 503 LAETLKYLYLLFSPSSVISLDEWVFNTEAHPLKI 536
>At1g27520.1 68414.m03355 glycoside hydrolase family 47 protein
Similar to gb|U04299 mannosyl-oligosaccharide
alpha-1,2-mannosidase from Mus musculus. ESTs gb|R84145
and gb|AA394707 come from this gene
Length = 574
Score = 74.9 bits (176), Expect = 3e-14
Identities = 40/94 (42%), Positives = 56/94 (59%)
Frame = +3
Query: 3 ETFESYFIMWRLTKQQRYRDWGWEAVQALEKHCRVDGGYTGIANVYHQNPQGDDVQQSFF 182
E ES F +++ TK Y D G V++L + +V GG+ + +V Q +D Q SFF
Sbjct: 388 ELAESTFYLYQATKDPWYLDVGESMVKSLNLYTKVPGGFASVRDV--TTMQLEDHQHSFF 445
Query: 183 LAETLKYLYLLFSDDTLVPLDEWVFNTEAHPLPI 284
LAET KYLYLLF DD+ V ++F TE HP+ +
Sbjct: 446 LAETCKYLYLLF-DDSFVAKRNYIFTTEGHPIQV 478
>At5g43710.1 68418.m05344 glycoside hydrolase family 47 protein
similar to mannosyl-oligosaccharide
1,2-alpha-mannosidase IB [Mus musculus][SP|P39098]
Length = 624
Score = 60.9 bits (141), Expect = 5e-10
Identities = 39/103 (37%), Positives = 59/103 (57%), Gaps = 6/103 (5%)
Frame = +3
Query: 3 ETFESYFIMWRLTKQQRYRDWGWEAVQALEKHCRVDGGYTGIANVYHQNPQGDDVQQSFF 182
E ES + +++ T+ RY D G + V +L+ + GY I +V + + +D +SFF
Sbjct: 376 ELIESTYWLYKATRDPRYLDAGRDFVASLQYGAKCPCGYCHITDV--ELHKQEDHMESFF 433
Query: 183 LAETLKYLYLLF----SDDTLVPLD--EWVFNTEAHPLPIKGQ 293
LAET+KYL+LLF D LV +++F+TE H LPI Q
Sbjct: 434 LAETVKYLWLLFDLAVDSDNLVDNGPYKYIFSTEGHLLPITPQ 476
>At4g12000.1 68417.m01909 expressed protein
Length = 306
Score = 30.3 bits (65), Expect = 0.76
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = +3
Query: 198 KYLYLLFSDDTLVPLDEWVFNTEAHPL 278
K+LY L D L+PL +W T HP+
Sbjct: 84 KWLYPLIMDKELIPLIKWEMETFTHPV 110
>At3g50370.1 68416.m05508 expressed protein
Length = 2179
Score = 29.1 bits (62), Expect = 1.8
Identities = 14/39 (35%), Positives = 20/39 (51%)
Frame = +3
Query: 48 QRYRDWGWEAVQALEKHCRVDGGYTGIANVYHQNPQGDD 164
++Y D GW Q +H G Y+ +QNP+GDD
Sbjct: 743 EQYGDPGWGQSQGRPRH----GPYSPYPEKLYQNPEGDD 777
>At1g60160.1 68414.m06777 potassium transporter family protein
similar to potassium transporter HAK2p [Mesembryanthemum
crystallinum] gi|14091471|gb|AAK53759; KUP/HAK/KT
Transporter family member, PMID:11500563; contains Pfam
profile PF02705: K+ potassium transporter
Length = 827
Score = 28.7 bits (61), Expect = 2.3
Identities = 9/20 (45%), Positives = 15/20 (75%)
Frame = -1
Query: 119 VPAVHSTMLLQCLNSLPAPV 60
+PA+HST++ C+ +P PV
Sbjct: 616 LPAIHSTIIFVCIKYVPVPV 635
>At5g09400.1 68418.m01089 potassium transporter family protein
similar to K+ transporter HAK5 [Arabidopsis thaliana]
GI:7108597; contains Pfam profile PF02705: K+ potassium
transporter; KUP/HAK/KT Transporter family member,
PMID:11500563; Note: possible sequencing error causes a
frameshift in the 4th exon|15810448|gb|AY056263
Length = 858
Score = 27.9 bits (59), Expect = 4.1
Identities = 9/22 (40%), Positives = 15/22 (68%)
Frame = -1
Query: 125 TRVPAVHSTMLLQCLNSLPAPV 60
T +PA+HS ++ C+ +P PV
Sbjct: 630 TTLPAIHSMVIFVCIKYVPVPV 651
>At4g35630.1 68417.m05060 phosphoserine aminotransferase,
chloroplast (PSAT) identical to Phosphoserine
aminotransferase, chloroplast precursor (PSAT)
(SP:Q96255)[Arabidopsis thaliana]; contains TIGRFAM
TIGR01364: phosphoserine aminotransferase; contains Pfam
PF00266: aminotransferase, class V
Length = 430
Score = 27.9 bits (59), Expect = 4.1
Identities = 10/38 (26%), Positives = 25/38 (65%)
Frame = +3
Query: 63 WGWEAVQALEKHCRVDGGYTGIANVYHQNPQGDDVQQS 176
WG +AV+ +K+C+ + ++G + Y + P ++++Q+
Sbjct: 171 WGDKAVKEAKKYCKTNVIWSGKSEKYTKVPSFEELEQT 208
>At5g42910.1 68418.m05231 basic leucine zipper transcription factor
(BZIP15) identical to cDNA basic leucine zipper
transcription factor (atbzip15 gene) GI:18656050, basic
leucine zipper transcription factor [Arabidopsis
thaliana] GI:18656051; contains Pfam profile PF00170:
bZIP transcription factor
Length = 370
Score = 27.5 bits (58), Expect = 5.4
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = +3
Query: 69 WEAVQALEKHCRVDGGYTGIANVYHQNPQGDDVQQSFFL 185
W+++ EKH +GG T I ++ Q G+ + FF+
Sbjct: 85 WKSITE-EKHTNNNGGVTNITHLQGQQTLGEITLEEFFI 122
>At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative
very strong similarity to SP|O23717 Proteasome subunit
beta type 5 precursor (EC 3.4.25.1) (20S proteasome
subunit E) (Proteasome epsilon chain) {Arabidopsis
thaliana}
Length = 273
Score = 27.1 bits (57), Expect = 7.1
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +3
Query: 108 DGGYTGIANVYHQNPQG 158
DG G+A+VYH PQG
Sbjct: 224 DGASGGVASVYHVGPQG 240
>At1g47765.1 68414.m05312 F-box family protein contains F-box domain
Pfam:PF00646
Length = 385
Score = 27.1 bits (57), Expect = 7.1
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = -2
Query: 481 ISCIEDKRSSEYTFIICCRVEHIKLQSWSS 392
I+C+E KR+++ +I C +E K WSS
Sbjct: 264 IACVE-KRTTKDDYIALCILEDAKKDKWSS 292
>At4g19530.1 68417.m02873 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1167
Score = 26.6 bits (56), Expect = 9.4
Identities = 14/42 (33%), Positives = 20/42 (47%)
Frame = -3
Query: 255 TPTRQGERGCRPRIGDIST*AFRQGRSSVERHHLEGSDGRRL 130
TPTR G G +ST R+ S++ H + +GR L
Sbjct: 1127 TPTRNGSSNTTTSSGSVSTNTIREEDSNI-LHEAQSQNGRGL 1167
>At1g15780.1 68414.m01893 expressed protein
Length = 1335
Score = 26.6 bits (56), Expect = 9.4
Identities = 14/48 (29%), Positives = 22/48 (45%)
Frame = -3
Query: 189 RQGRSSVERHHLEGSDGRRLRYPCTRRPLDNASPMPEQPPSPSRGSAA 46
RQ +SS+ R H + + + P + SP+ +QP R AA
Sbjct: 317 RQHQSSMLRQHPQSQQASGIHQQQSSLPQQSISPLQQQPTQLMRQQAA 364
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,642,933
Number of Sequences: 28952
Number of extensions: 217050
Number of successful extensions: 732
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 689
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 726
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 888318720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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