Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0006_M05
(591 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g24230.1 68418.m02851 expressed protein 32 0.25
At4g03260.1 68417.m00445 leucine-rich repeat family protein cont... 32 0.25
At4g10050.1 68417.m01643 hydrolase, alpha/beta fold family prote... 31 0.57
At5g67040.1 68418.m08452 hypothetical protein contains Pfam prof... 30 1.3
At1g10040.1 68414.m01132 expressed protein non-consensus GC dono... 30 1.3
At5g65960.1 68418.m08303 expressed protein 29 3.1
At4g00230.1 68417.m00025 subtilisin-like serine endopeptidase (X... 29 3.1
At5g24220.1 68418.m02850 lipase class 3-related 28 5.4
At4g10550.1 68417.m01727 subtilase family protein contains simil... 28 5.4
At4g10260.1 68417.m01684 pfkB-type carbohydrate kinase family pr... 28 5.4
At5g59400.2 68418.m07444 expressed protein predicted protein, Ar... 27 7.1
At5g59400.1 68418.m07443 expressed protein predicted protein, Ar... 27 7.1
At4g10955.1 68417.m01782 lipase class 3 family protein contains ... 27 7.1
At1g26120.1 68414.m03188 esterase-related contains similaity to ... 27 7.1
At5g51270.1 68418.m06356 protein kinase family protein contains ... 27 9.4
At4g00570.1 68417.m00080 malate oxidoreductase, putative similar... 27 9.4
At2g34500.1 68415.m04237 cytochrome P450 family protein similar ... 27 9.4
At1g68960.1 68414.m07893 hypothetical protein contains Pfam prof... 27 9.4
At1g06520.1 68414.m00691 phospholipid/glycerol acyltransferase f... 27 9.4
At1g05670.1 68414.m00588 UDP-glucoronosyl/UDP-glucosyl transfera... 27 9.4
>At5g24230.1 68418.m02851 expressed protein
Length = 369
Score = 32.3 bits (70), Expect = 0.25
Identities = 29/87 (33%), Positives = 45/87 (51%)
Frame = +1
Query: 253 LHSTTYVRFIGEKLGEVLAAMAKGGMSPDAIHLIGHSLGSHISGFAGKTFTTLTGLQVGR 432
LH +T RF+ + + +A+AK G + A+ L GHSLG+ ++ AGK T +G +
Sbjct: 149 LHDST--RFV-HAIQVIQSAVAKTGNA--AVWLAGHSLGAAVALLAGKIMTR-SGFPLES 202
Query: 433 ISGLDPAGPCFSNIPAELRLKHTDAKY 513
P FS+IP E +K K+
Sbjct: 203 YL----FNPPFSSIPIEKLVKSEKLKH 225
>At4g03260.1 68417.m00445 leucine-rich repeat family protein
contains leucine rich repeat (LRR) domains, Pfam:PF00560
Length = 677
Score = 32.3 bits (70), Expect = 0.25
Identities = 17/54 (31%), Positives = 28/54 (51%)
Frame = -1
Query: 258 MEVQPTDERAAVQGQDVKFTLV*ETIADDIEVLLSRIVGEAVNEDDQLPMRVVI 97
+E + T+++A G+D KF + + + D V LS E DD+LP V+
Sbjct: 312 LEGETTNQQAEFSGKDSKFKRIEDWVNDLQHVNLSEEADEITGYDDELPREPVV 365
>At4g10050.1 68417.m01643 hydrolase, alpha/beta fold family protein
similar to protein phosphatase methylesterase-1 [Homo
sapiens] GI:5533003; contains Pfam profile PF00561:
hydrolase, alpha/beta fold family
Length = 350
Score = 31.1 bits (67), Expect = 0.57
Identities = 20/46 (43%), Positives = 28/46 (60%), Gaps = 3/46 (6%)
Frame = +1
Query: 298 EVLAAMAK-GGMSPDAIHLIGHSLGSHIS--GFAGKTFTTLTGLQV 426
+VLA + + G SP AI L+GHS+G ++ A KT +L GL V
Sbjct: 134 DVLAVIKELYGDSPPAIVLVGHSMGGSVAVQVAANKTLPSLAGLVV 179
>At5g67040.1 68418.m08452 hypothetical protein contains Pfam profile
PF03478: Protein of unknown function (DUF295)
Length = 165
Score = 29.9 bits (64), Expect = 1.3
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +1
Query: 277 FIGEKLGEVLAAMAKGGMSPDAIHLIGHSLGSH 375
F+G L A G+ P++I+ IGH LGS+
Sbjct: 104 FLGASEAFCLTASMYPGLKPNSIYYIGHGLGSY 136
>At1g10040.1 68414.m01132 expressed protein non-consensus GC donor
splice site at exon boundary 21576
Length = 412
Score = 29.9 bits (64), Expect = 1.3
Identities = 19/48 (39%), Positives = 28/48 (58%), Gaps = 3/48 (6%)
Frame = +1
Query: 256 HST-TY--VRFIGEKLGEVLAAMAKGGMSPDAIHLIGHSLGSHISGFA 390
HST T+ V +GE+L E + ++ K S I +GHSLG I+ +A
Sbjct: 120 HSTQTFDGVDVMGERLAEEVRSVIKRHPSLQKISFVGHSLGGLIARYA 167
>At5g65960.1 68418.m08303 expressed protein
Length = 393
Score = 28.7 bits (61), Expect = 3.1
Identities = 19/67 (28%), Positives = 31/67 (46%)
Frame = -1
Query: 423 LKSGKRCERLTSET*YVRAEAVPYQMNRIGTHTALCHSREDFTELLSYEPNVRGRMEVQP 244
L SG R E L ++T A+ +GT C+ ED +L+S N+R + + P
Sbjct: 309 LPSGSRLE-LQNDT----DRAIVTDEKPLGTENEKCYEFEDVEQLMSEIGNIRDNLRLMP 363
Query: 243 TDERAAV 223
+R +
Sbjct: 364 DFQRREI 370
>At4g00230.1 68417.m00025 subtilisin-like serine endopeptidase
(XSP1) identical to subtilisin-type serine endopeptidase
XSP1 GI:6708179 from [Arabidopsis thaliana]
Length = 749
Score = 28.7 bits (61), Expect = 3.1
Identities = 23/94 (24%), Positives = 41/94 (43%), Gaps = 9/94 (9%)
Frame = +1
Query: 298 EVLAAMAKGGMSPDAIHLIGHSLGSHISGFAG-----KTFT----TLTGLQVGRISGLDP 450
+V+ + G++PD+ + H LG + + G K FT + G + + G P
Sbjct: 138 DVIIGVLDTGITPDSESFLDHGLGPPPAKWKGSCGPYKNFTGCNNKIIGAKYFKHDGNVP 197
Query: 451 AGPCFSNIPAELRLKHTDAKYVDVIHTDGGVYGL 552
AG S I + HT + V+ + +YG+
Sbjct: 198 AGEVRSPIDIDGHGTHTSSTVAGVLVANASLYGI 231
>At5g24220.1 68418.m02850 lipase class 3-related
Length = 376
Score = 27.9 bits (59), Expect = 5.4
Identities = 14/26 (53%), Positives = 17/26 (65%)
Frame = +1
Query: 331 SPDAIHLIGHSLGSHISGFAGKTFTT 408
S AI L GHSLG+ + AGKT T+
Sbjct: 171 SEAAIWLAGHSLGAALVLLAGKTMTS 196
>At4g10550.1 68417.m01727 subtilase family protein contains
similarity to subtilisin-like protease AIR3 GI:4218991
from [Arabidopsis thaliana]
Length = 778
Score = 27.9 bits (59), Expect = 5.4
Identities = 19/76 (25%), Positives = 34/76 (44%), Gaps = 1/76 (1%)
Frame = +1
Query: 280 IGEKLGEVLAAMAKGGMSPDAIHLIGHSLGSH-ISGFAGKTFTTLTGLQVGRISGLDPAG 456
+G+ +G +A + G + A ++ + + +S A T TT + +SG A
Sbjct: 500 VGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAATTNTTFSDQGFIMLSGTSMAA 559
Query: 457 PCFSNIPAELRLKHTD 504
P S + A L+ H D
Sbjct: 560 PAISGVAALLKALHRD 575
>At4g10260.1 68417.m01684 pfkB-type carbohydrate kinase family
protein contains Pfam profile: PF00294 pfkB family
carbohydrate kinase
Length = 324
Score = 27.9 bits (59), Expect = 5.4
Identities = 16/38 (42%), Positives = 24/38 (63%)
Frame = -1
Query: 330 HTALCHSREDFTELLSYEPNVRGRMEVQPTDERAAVQG 217
H A + +D LLSY+PNV R+ + P+ E AA++G
Sbjct: 150 HMAAMKTAKDAGVLLSYDPNV--RLPLWPSTE-AAIEG 184
>At5g59400.2 68418.m07444 expressed protein predicted protein,
Arabidopsis thaliana
Length = 301
Score = 27.5 bits (58), Expect = 7.1
Identities = 16/26 (61%), Positives = 17/26 (65%)
Frame = +2
Query: 248 CTSIRPRTFGS*ERSSVKSSRLWQRA 325
CTSIRPR GS S V+ SRL RA
Sbjct: 5 CTSIRPRLIGS--SSIVELSRLINRA 28
>At5g59400.1 68418.m07443 expressed protein predicted protein,
Arabidopsis thaliana
Length = 299
Score = 27.5 bits (58), Expect = 7.1
Identities = 16/26 (61%), Positives = 17/26 (65%)
Frame = +2
Query: 248 CTSIRPRTFGS*ERSSVKSSRLWQRA 325
CTSIRPR GS S V+ SRL RA
Sbjct: 5 CTSIRPRLIGS--SSIVELSRLINRA 28
>At4g10955.1 68417.m01782 lipase class 3 family protein contains
Pfam profile PF01764: Lipase
Length = 350
Score = 27.5 bits (58), Expect = 7.1
Identities = 17/49 (34%), Positives = 28/49 (57%)
Frame = +1
Query: 253 LHSTTYVRFIGEKLGEVLAAMAKGGMSPDAIHLIGHSLGSHISGFAGKT 399
LH+TT + + ++A++ GG S + L GHSLG+ ++ GKT
Sbjct: 136 LHTTTRFEIAIQAVRNIVASV--GGSS---VWLAGHSLGASMALLTGKT 179
>At1g26120.1 68414.m03188 esterase-related contains similaity to
esterase 6 GI:606998 from [Drosophila simulans] and
esterase GI:12584120 from [Sphingomonas elodea]
Length = 476
Score = 27.5 bits (58), Expect = 7.1
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = +1
Query: 313 MAKGGMSPDAIHLIGHSLGSHIS 381
+A+ G PD I+L+G S G+HI+
Sbjct: 270 IAEYGGDPDRIYLMGQSAGAHIA 292
>At5g51270.1 68418.m06356 protein kinase family protein contains
Pfam profile: PF00069 Eukaryotic protein kinase domain
Length = 819
Score = 27.1 bits (57), Expect = 9.4
Identities = 15/60 (25%), Positives = 30/60 (50%)
Frame = +3
Query: 273 SVHRREAR*SPRGYGKGRYESRCDSSDRAQPRLSHIRFRW*DVHNAYRTSSRQDIRTGSG 452
+ RREA + + + + + + S P+L + F W ++ NA +S +D++ G G
Sbjct: 413 AAQRREAE--MKATHEAKEKEKLEESSLVAPKLQYQEFTWEEIINA-TSSFSEDLKIGMG 469
>At4g00570.1 68417.m00080 malate oxidoreductase, putative similar to
NAD-dependent malic enzyme 59 kDa isoform, mitochondrial
precursor (EC 1.1.1.39) (NAD-ME) (SP:P37225) {Solanum
tuberosum}
Length = 607
Score = 27.1 bits (57), Expect = 9.4
Identities = 22/73 (30%), Positives = 33/73 (45%), Gaps = 2/73 (2%)
Frame = +1
Query: 277 FIGEKLGEVLAAMAKGGMSPDAIHLIGHSLGSHISGFAGKTFTTLT--GLQVGRISGLDP 450
F+ +K+ V A A G++ A+ + G S A K F + GL + LDP
Sbjct: 328 FVNQKIVVVGAGSAGLGVTKMAVQAVARMAGISESE-ATKNFYLIDKDGLVTTERTKLDP 386
Query: 451 AGPCFSNIPAELR 489
F+ PAE+R
Sbjct: 387 GAVLFAKNPAEIR 399
>At2g34500.1 68415.m04237 cytochrome P450 family protein similar to
Cytochrome P450 61 (C-22 sterol desaturase) (SP:P54781)
{Saccharomyces cerevisiae}
Length = 495
Score = 27.1 bits (57), Expect = 9.4
Identities = 10/17 (58%), Positives = 11/17 (64%)
Frame = +1
Query: 334 PDAIHLIGHSLGSHISG 384
PDA HLIGH G + G
Sbjct: 101 PDAFHLIGHPFGKKLFG 117
>At1g68960.1 68414.m07893 hypothetical protein contains Pfam profile
PF03478: Protein of unknown function (DUF295)
Length = 376
Score = 27.1 bits (57), Expect = 9.4
Identities = 14/46 (30%), Positives = 23/46 (50%)
Frame = +1
Query: 277 FIGEKLGEVLAAMAKGGMSPDAIHLIGHSLGSHISGFAGKTFTTLT 414
F+GE L+A G++P++++ GH G I A T L+
Sbjct: 313 FLGENESFCLSAKDFPGLNPNSVYFAGHGSGYGIWDLASCTIRYLS 358
>At1g06520.1 68414.m00691 phospholipid/glycerol acyltransferase
family protein contains Pfam profile PF01553:
Acyltransferase
Length = 585
Score = 27.1 bits (57), Expect = 9.4
Identities = 9/25 (36%), Positives = 18/25 (72%)
Frame = -3
Query: 400 TSYQRNLICESRGCALSDESHRDSY 326
T YQ L+ ++R C++S + +RD++
Sbjct: 82 TKYQELLLDKNRACSVSSDHYRDTF 106
>At1g05670.1 68414.m00588 UDP-glucoronosyl/UDP-glucosyl transferase
family protein similar to UDP-glucose:salicylic acid
glucosyltransferase [Nicotiana tabacum] GI:7385017;
contains Pfam profiles PF00201: UDP-glucoronosyl and
UDP-glucosyl transferase, PF01535: PPR repeat
Length = 1184
Score = 27.1 bits (57), Expect = 9.4
Identities = 16/53 (30%), Positives = 26/53 (49%)
Frame = +1
Query: 91 SRDYDPHRKLIIFIHGFTDDPTKENFNVISNGFLNQGEFNILALDGSSLIRWL 249
S + D ++++ + G PT FNV+ NGF G + DG L+ W+
Sbjct: 982 SGEMDKAQEILKEMLGKGLQPTIVTFNVLMNGFCLHG----MLEDGEKLLNWM 1030
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,861,838
Number of Sequences: 28952
Number of extensions: 286355
Number of successful extensions: 869
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 842
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 868
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1171109464
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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