Samia BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0006_L19
         (529 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g51590.1 68414.m05808 mannosyl-oligosaccharide 1,2-alpha-mann...   109   1e-24
At3g21160.1 68416.m02673 mannosyl-oligosaccharide 1,2-alpha-mann...   106   8e-24
At5g43710.1 68418.m05344 glycoside hydrolase family 47 protein s...    64   4e-11
At1g27520.1 68414.m03355 glycoside hydrolase family 47 protein S...    64   7e-11
At1g30000.1 68414.m03669 glycoside hydrolase family 47 protein s...    47   7e-06
At1g67410.1 68414.m07672 exostosin family protein contains Pfam ...    32   0.21 
At2g19050.1 68415.m02225 GDSL-motif lipase/hydrolase family prot...    27   5.9  
At5g51020.1 68418.m06325 expressed protein similar to unknown pr...    27   7.8  
At2g19060.1 68415.m02226 GDSL-motif lipase/hydrolase family prot...    27   7.8  

>At1g51590.1 68414.m05808 mannosyl-oligosaccharide
           1,2-alpha-mannosidase, putative similar to
           mannosyl-oligosaccharide 1,2-alpha-mannosidase [Glycine
           max][GI:6552504]
          Length = 560

 Score =  109 bits (261), Expect = 1e-24
 Identities = 49/97 (50%), Positives = 71/97 (73%)
 Frame = +2

Query: 140 IKTLYLMGLNDEFREGRDWVAEHLHFNEVDTELSVFETTIRFIGGLLSCYSLTGDTVFRD 319
           + TLY+MGL+++F++ R+WVA  L F++ D + S+FETTIR +GGLLS Y L+GD +F +
Sbjct: 144 LDTLYIMGLDEQFQKAREWVASSLDFDK-DYDASMFETTIRVVGGLLSAYDLSGDKMFLE 202

Query: 320 KAVEVADALLPAFQTPTGLPYALINPSTKASKQYHWA 430
           KA ++AD LLPA+ TPTG+PY +IN     +    WA
Sbjct: 203 KAKDIADRLLPAWNTPTGIPYNIINLRNGNAHNPSWA 239


>At3g21160.1 68416.m02673 mannosyl-oligosaccharide
           1,2-alpha-mannosidase, putative similar to
           mannosyl-oligosaccharide 1,2-alpha-mannosidase [Glycine
           max][GI:6552504]
          Length = 572

 Score =  106 bits (255), Expect = 8e-24
 Identities = 48/98 (48%), Positives = 71/98 (72%)
 Frame = +2

Query: 140 IKTLYLMGLNDEFREGRDWVAEHLHFNEVDTELSVFETTIRFIGGLLSCYSLTGDTVFRD 319
           + TLY+MGL+++F++ R+WVA  L F++ D   S+FETTIR +GGLLS Y L+GD +F +
Sbjct: 145 LDTLYIMGLDEQFQKAREWVASSLDFDK-DYAASMFETTIRVVGGLLSAYDLSGDKIFLE 203

Query: 320 KAVEVADALLPAFQTPTGLPYALINPSTKASKQYHWAG 433
           KA+++AD LLPA+ T +G+PY +IN     +    WAG
Sbjct: 204 KAMDIADRLLPAWDTQSGIPYNIINLKHGNAHNPTWAG 241


>At5g43710.1 68418.m05344 glycoside hydrolase family 47 protein
           similar to mannosyl-oligosaccharide
           1,2-alpha-mannosidase IB [Mus musculus][SP|P39098]
          Length = 624

 Score = 64.5 bits (150), Expect = 4e-11
 Identities = 35/96 (36%), Positives = 57/96 (59%), Gaps = 11/96 (11%)
 Frame = +2

Query: 140 IKTLYLMGLNDEFREGRDWVAEHLHFNEVDTELSVFETTIRFIGGLLSCYSLTGDTV--- 310
           + TL L+G  + F    +W+ ++L FN ++  +SVFETTIR +GGLLS + +  D     
Sbjct: 87  LDTLALLGDRERFTSSVEWIGKNLQFN-INKTVSVFETTIRVLGGLLSAHLIASDYATGM 145

Query: 311 ----FRDKAVEVADAL----LPAFQTPTGLPYALIN 394
               + ++ + +A+ L    LPAF TPTG+P+  +N
Sbjct: 146 RIPSYNNELLVLAENLARRMLPAFDTPTGIPFGSVN 181


>At1g27520.1 68414.m03355 glycoside hydrolase family 47 protein
           Similar to gb|U04299 mannosyl-oligosaccharide
           alpha-1,2-mannosidase from Mus musculus. ESTs gb|R84145
           and gb|AA394707 come from this gene
          Length = 574

 Score = 63.7 bits (148), Expect = 7e-11
 Identities = 36/96 (37%), Positives = 60/96 (62%), Gaps = 11/96 (11%)
 Frame = +2

Query: 140 IKTLYLMGLNDEFREGRDWVAEHLHFNEVDTELSVFETTIRFIGGLLSCYSLTGDT---- 307
           + +L ++G + EF +G  W++E+L F ++D  +++FE  IR +GGL+S + L  D     
Sbjct: 99  LSSLAILGNSTEFEKGVLWLSENLTF-DIDARVNLFECNIRVLGGLISAHLLAIDPNNRL 157

Query: 308 ---VFRDKAVEVADAL----LPAFQTPTGLPYALIN 394
               + ++ + +A+ L    LPAF+TPTGLPYA IN
Sbjct: 158 IQGSYNNQLLRLAEDLGKRFLPAFETPTGLPYAWIN 193


>At1g30000.1 68414.m03669 glycoside hydrolase family 47 protein
           similar to GI:5579331 from [Homo sapiens]; contains Pfam
           profile PF01532: Glycosyl hydrolase family 47
          Length = 624

 Score = 47.2 bits (107), Expect = 7e-06
 Identities = 33/97 (34%), Positives = 51/97 (52%), Gaps = 16/97 (16%)
 Frame = +2

Query: 140 IKTLYLMGLNDEFREGRDWVAEHLHFN-EVDTELSVFETTIRFIGGLLSCYSLTG----- 301
           + T  +MGL++   E   WV  HL        ++++FETTIR +GGLLS Y L+G     
Sbjct: 175 LDTAMIMGLDNIVSEAGSWVETHLLERISQKGQVNLFETTIRVLGGLLSAYHLSGGEQGT 234

Query: 302 ---------DTVFRDKAVEVADALLPAF-QTPTGLPY 382
                      ++ + A ++AD LL AF  +PT +P+
Sbjct: 235 VNMTHVGPKPVIYLNIAKDLADRLLSAFTSSPTPVPF 271


>At1g67410.1 68414.m07672 exostosin family protein contains Pfam
           profile: PF03016 Exostosin family
          Length = 430

 Score = 32.3 bits (70), Expect = 0.21
 Identities = 14/44 (31%), Positives = 25/44 (56%)
 Frame = +3

Query: 180 ERDETGSPNICISTKSIRSYRYSRQPSGSSVDSCRVIR*PVTQC 311
           E+    + NI +ST+ +RS ++   P+G +  SCR+    V+ C
Sbjct: 281 EKSVATTQNIKVSTEGMRSSKFCLHPAGDTPSSCRLFDAIVSHC 324


>At2g19050.1 68415.m02225 GDSL-motif lipase/hydrolase family protein
           low similarity to SP|P40603 Anter-specific proline-rich
           protein APG (Protein CEX) (Fragment) {Brassica napus};
           contains Pfam profile PF00657: GDSL-like
           Lipase/Acylhydrolase
          Length = 349

 Score = 27.5 bits (58), Expect = 5.9
 Identities = 14/49 (28%), Positives = 24/49 (48%)
 Frame = +2

Query: 239 SVFETTIRFIGGLLSCYSLTGDTVFRDKAVEVADALLPAFQTPTGLPYA 385
           + F TT   +G  + CY + GD+VF +    V +       +P G+ +A
Sbjct: 16  TAFATTEAALGQRVPCYFVFGDSVFDNGNNNVLNTSAKVNYSPYGIDFA 64


>At5g51020.1 68418.m06325 expressed protein similar to unknown
           protein (pir||S76207)
          Length = 269

 Score = 27.1 bits (57), Expect = 7.8
 Identities = 11/39 (28%), Positives = 20/39 (51%)
 Frame = -1

Query: 328 HCFIPEHCVTGQRITRQESTDEPDGCLEYR*LRIDFVEM 212
           H  +    +TG++ TR++++ +PD     R L     EM
Sbjct: 48  HARVVSRSLTGEKFTREQASRDPDNYFNIRMLSCPAAEM 86


>At2g19060.1 68415.m02226 GDSL-motif lipase/hydrolase family protein
           similar to family II lipases EXL6 GI:15054390, EXL1
           GI:15054382, EXL2 GI:15054384 from [Arabidopsis
           thaliana]; contains Pfam profile PF00657: GDSL-like
           Lipase/Acylhydrolase
          Length = 349

 Score = 27.1 bits (57), Expect = 7.8
 Identities = 13/39 (33%), Positives = 20/39 (51%)
 Frame = +2

Query: 269 GGLLSCYSLTGDTVFRDKAVEVADALLPAFQTPTGLPYA 385
           G L+ CY + GD+VF +      D L     +P G+ +A
Sbjct: 25  GQLVPCYFVFGDSVFDNGNNNELDTLAKVNYSPYGIDFA 63


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,277,301
Number of Sequences: 28952
Number of extensions: 202947
Number of successful extensions: 507
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 495
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 501
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 977150592
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)