Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0006_J01
(466 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g09590.1 68418.m01110 heat shock protein 70 / HSP70 (HSC70-5)... 31 0.51
At5g49910.1 68418.m06180 heat shock protein 70 / HSP70 (HSC70-7)... 30 0.89
At4g24280.1 68417.m03486 heat shock protein 70, putative / HSP70... 30 0.89
At3g10070.1 68416.m01207 transcription initiation factor IID (TF... 29 1.5
At3g07430.1 68416.m00886 YGGT family protein contains Pfam profi... 28 2.7
At2g34050.1 68415.m04169 expressed protein contains Pfam PS00030... 28 2.7
At1g16030.1 68414.m01924 heat shock protein 70, putative / HSP70... 27 4.7
At5g02490.1 68418.m00182 heat shock cognate 70 kDa protein 2 (HS... 27 6.2
At4g37910.1 68417.m05361 heat shock protein 70, mitochondrial, p... 27 6.2
At3g12580.1 68416.m01567 heat shock protein 70, putative / HSP70... 27 6.2
At3g09440.1 68416.m01121 heat shock cognate 70 kDa protein 3 (HS... 27 6.2
At2g39550.1 68415.m04852 geranylgeranyl transferase type I beta ... 27 6.2
At1g56410.1 68414.m06487 heat shock cognate 70 kDa protein, puta... 27 6.2
At4g31390.1 68417.m04452 ABC1 family protein contains Pfam domai... 27 8.3
At2g19230.1 68415.m02245 leucine-rich repeat protein kinase, put... 27 8.3
At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)... 27 8.3
>At5g09590.1 68418.m01110 heat shock protein 70 / HSP70 (HSC70-5)
identical to heat shock protein 70 [Arabidopsis
thaliana] GI:6746590
Length = 682
Score = 30.7 bits (66), Expect = 0.51
Identities = 20/58 (34%), Positives = 27/58 (46%), Gaps = 3/58 (5%)
Frame = +3
Query: 87 GRDPSRSINADEAATMGAVYRAAALATGYKVAALNVRDAVILPIQV---VFTRHVDGN 251
G+ PS+ +N DEA MGA + L K L + L I+ VFTR + N
Sbjct: 407 GKSPSKGVNPDEAVAMGAALQGGILRGDVKELLLLDVTPLSLGIETLGGVFTRLITRN 464
>At5g49910.1 68418.m06180 heat shock protein 70 / HSP70 (HSC70-7)
identical to heat shock protein 70 [Arabidopsis
thaliana] GI:6746592
Length = 718
Score = 29.9 bits (64), Expect = 0.89
Identities = 11/27 (40%), Positives = 18/27 (66%)
Frame = +3
Query: 84 TGRDPSRSINADEAATMGAVYRAAALA 164
TG++P+ S+N DE +GA +A L+
Sbjct: 428 TGKEPNVSVNPDEVVALGAAVQAGVLS 454
>At4g24280.1 68417.m03486 heat shock protein 70, putative / HSP70,
putative strong similarity to heat shock protein 70
[Arabidopsis thaliana] GI:6746592; similar to heat shock
70 protein - Spinacia oleracea,PID:g2654208
Length = 718
Score = 29.9 bits (64), Expect = 0.89
Identities = 11/27 (40%), Positives = 18/27 (66%)
Frame = +3
Query: 84 TGRDPSRSINADEAATMGAVYRAAALA 164
TG++P+ ++N DE +GA +A LA
Sbjct: 428 TGKEPNVTVNPDEVVALGAAVQAGVLA 454
>At3g10070.1 68416.m01207 transcription initiation factor IID
(TFIID) subunit A family protein similar to hypothetical
protein GB:CAB10099 [Schizosaccharomyces pombe];
contains Pfam profile PF03847: Transcription initiation
factor TFIID subunit A
Length = 539
Score = 29.1 bits (62), Expect = 1.5
Identities = 20/54 (37%), Positives = 27/54 (50%)
Frame = -3
Query: 164 SQSSSPVNSAHGGGLVGVDGPRRIAPGGGAQRADDGRSPSRAPRQHEPSARPGA 3
S SS+P +S+ + + G +R G A SPS P QH PSA PG+
Sbjct: 117 SSSSAPASSSSS---LPISGQQRGGMAIGVP-ASPIPSPSPTPSQHSPSAFPGS 166
>At3g07430.1 68416.m00886 YGGT family protein contains Pfam profile
PF02325: YGGT family (unknown function)
Length = 232
Score = 28.3 bits (60), Expect = 2.7
Identities = 14/45 (31%), Positives = 20/45 (44%)
Frame = +3
Query: 177 VAALNVRDAVILPIQVVFTRHVDGNDQLVKRTLFGPMNPYPQKKV 311
+ AL +R V LP R +Q LF P+NP+P +
Sbjct: 4 ITALTLRSPVYLPSSATSPRFHGFTNQPPPARLFFPLNPFPSLSI 48
>At2g34050.1 68415.m04169 expressed protein contains Pfam PS00030:
Eukaryotic putative RNA-binding region RNP-1 signature
Length = 248
Score = 28.3 bits (60), Expect = 2.7
Identities = 19/68 (27%), Positives = 30/68 (44%), Gaps = 2/68 (2%)
Frame = +3
Query: 150 AAALATGYKVAALNVRDAVILPIQVVFTRHVDGNDQ--LVKRTLFGPMNPYPQKKVITFN 323
A L T + +D V + VVFT + + +++ +N K + FN
Sbjct: 169 APYLTTTFYTELSETKDLVFIRGDVVFTSKLTDEEAKWIMETAQSFYLNDSRYKLLERFN 228
Query: 324 KHTDDFDF 347
KHT DF+F
Sbjct: 229 KHTHDFEF 236
>At1g16030.1 68414.m01924 heat shock protein 70, putative / HSP70,
putative similar to heat shock protein hsp70 GI:1771478
from [Pisum sativum]
Length = 646
Score = 27.5 bits (58), Expect = 4.7
Identities = 17/44 (38%), Positives = 26/44 (59%)
Frame = +3
Query: 87 GRDPSRSINADEAATMGAVYRAAALATGYKVAALNVRDAVILPI 218
G++ +SIN DEA GA +AA L TG + V+D ++L +
Sbjct: 361 GKELCKSINPDEAVAYGAAVQAAIL-TG--EGSEKVQDLLLLDV 401
>At5g02490.1 68418.m00182 heat shock cognate 70 kDa protein 2
(HSC70-2) (HSP70-2) identical to SP|P22954 Heat shock
cognate 70 kDa protein 2 (Hsc70.2) {Arabidopsis
thaliana}
Length = 653
Score = 27.1 bits (57), Expect = 6.2
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +3
Query: 87 GRDPSRSINADEAATMGAVYRAAALA 164
G++ +SIN DEA GA +AA L+
Sbjct: 362 GKELCKSINPDEAVAYGAAVQAAILS 387
>At4g37910.1 68417.m05361 heat shock protein 70, mitochondrial,
putative / HSP70, mitochondrial, putative strong
similarity to SP|Q01899 Heat shock 70 kDa protein,
mitochondrial precursor {Phaseolus vulgaris}
Length = 682
Score = 27.1 bits (57), Expect = 6.2
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = +3
Query: 87 GRDPSRSINADEAATMGAVYRAAAL 161
G+ P + +N DEA MGA + L
Sbjct: 402 GKSPCKGVNPDEAVAMGAAIQGGIL 426
>At3g12580.1 68416.m01567 heat shock protein 70, putative / HSP70,
putative strong similarity to heat shock protein
GI:425194 [Spinacia oleracea]
Length = 650
Score = 27.1 bits (57), Expect = 6.2
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +3
Query: 87 GRDPSRSINADEAATMGAVYRAAALA 164
G++ +SIN DEA GA +AA L+
Sbjct: 362 GKELCKSINPDEAVAYGAAVQAAILS 387
>At3g09440.1 68416.m01121 heat shock cognate 70 kDa protein 3
(HSC70-3) (HSP70-3) identical to SP|O65719 Heat shock
cognate 70 kDa protein 3 (Hsc70.3) {Arabidopsis
thaliana}
Length = 649
Score = 27.1 bits (57), Expect = 6.2
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +3
Query: 87 GRDPSRSINADEAATMGAVYRAAALA 164
G++ +SIN DEA GA +AA L+
Sbjct: 362 GKELCKSINPDEAVAYGAAVQAAILS 387
>At2g39550.1 68415.m04852 geranylgeranyl transferase type I beta
subunit (GGT-IB) nearly identical to GI:11878247
Length = 375
Score = 27.1 bits (57), Expect = 6.2
Identities = 16/42 (38%), Positives = 22/42 (52%), Gaps = 1/42 (2%)
Frame = -2
Query: 375 YGLVPRSLHRNRNRQCAY*KL-LLSFVGMDSLVRKVSSSPID 253
+GL+P S CA L L+ ++G+D L SSS ID
Sbjct: 223 FGLIPGSESHGGATYCAIASLRLMGYIGVDLLSNDSSSSIID 264
>At1g56410.1 68414.m06487 heat shock cognate 70 kDa protein,
putative / HSC70, putative / HSP70, putative strong
similarity to heat shock cognate 70 kd protein 1
SP:P22953 [Arabidopsis thaliana (Mouse-ear cress)]
Length = 617
Score = 27.1 bits (57), Expect = 6.2
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +3
Query: 87 GRDPSRSINADEAATMGAVYRAAALA 164
G++ +SIN DEA GA +AA L+
Sbjct: 362 GKELCKSINPDEAVAYGAAVQAAILS 387
>At4g31390.1 68417.m04452 ABC1 family protein contains Pfam domain,
PF03109: ABC1 family
Length = 682
Score = 26.6 bits (56), Expect = 8.3
Identities = 16/32 (50%), Positives = 20/32 (62%)
Frame = +3
Query: 123 AATMGAVYRAAALATGYKVAALNVRDAVILPI 218
AA++G VYRA ATG V A+ V+ I PI
Sbjct: 244 AASLGQVYRATLRATGEDV-AIKVQRPQIEPI 274
>At2g19230.1 68415.m02245 leucine-rich repeat protein kinase,
putative similar to light repressible receptor protein
kinase [Arabidopsis thaliana] gi|1321686|emb|CAA66376;
contains leucine rich repeat (LRR) domains,
Pfam:PF00560; contains protein kinase domain,
Pfam:PF00069
Length = 877
Score = 26.6 bits (56), Expect = 8.3
Identities = 15/64 (23%), Positives = 32/64 (50%), Gaps = 3/64 (4%)
Frame = +3
Query: 258 LVKRTLFGPMNPYPQKKVITFNKHTDDFDFYV--NYAELDHIPSTELQYIGT-LNLTKVS 428
+V T PMN ++ ++ + ++ FY+ ++AE++ +PS E + LN ++
Sbjct: 251 VVMSTAVAPMNSSIEQIMVYWEPRDPNWKFYIYIHFAEVEKLPSNETREFSVFLNKEQID 310
Query: 429 LVGV 440
V
Sbjct: 311 TTSV 314
>At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)
Similar to Arabidopsis luminal binding protein
(gb|D89342); contains Pfam domain PF00012: dnaK protein
Length = 678
Score = 26.6 bits (56), Expect = 8.3
Identities = 10/26 (38%), Positives = 15/26 (57%)
Frame = +3
Query: 87 GRDPSRSINADEAATMGAVYRAAALA 164
G++PS+ N DEA GA + L+
Sbjct: 401 GKEPSKGTNPDEAVAYGAAVQGGVLS 426
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,247,383
Number of Sequences: 28952
Number of extensions: 192506
Number of successful extensions: 550
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 536
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 546
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 782033640
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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