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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0006_I19
         (468 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g02580.1 68417.m00352 NADH-ubiquinone oxidoreductase 24 kDa s...   144   3e-35
At5g22330.1 68418.m02605 TATA box-binding protein-interacting pr...    32   0.22 
At1g01450.1 68414.m00060 protein kinase-related contains eukaryo...    29   2.1  
At5g57200.1 68418.m07145 epsin N-terminal homology (ENTH) domain...    28   3.6  
At1g08260.1 68414.m00911 DNA-directed DNA polymerase epsilon cat...    28   3.6  
At5g38070.1 68418.m04587 zinc finger (C3HC4-type RING finger) fa...    27   4.8  
At4g25940.1 68417.m03731 epsin N-terminal homology (ENTH) domain...    27   6.3  
At1g74710.2 68414.m08655 isochorismate synthase 1 (ICS1) / isoch...    27   6.3  
At1g02940.1 68414.m00261 glutathione S-transferase, putative sim...    27   6.3  
At4g22330.1 68417.m03228 alkaline phytoceramidase family / aPHC ...    27   8.4  

>At4g02580.1 68417.m00352 NADH-ubiquinone oxidoreductase 24 kDa
           subunit, putative similar to NADH-ubiquinone
           oxidoreductase 24 kDa subunit, mitochondrial precursor
           (EC 1.6.5.3) (EC 1.6.99.3) (Polypeptide II)
           (Swiss-Prot:P04394) [Bos taurus]
          Length = 255

 Score =  144 bits (348), Expect = 3e-35
 Identities = 60/110 (54%), Positives = 86/110 (78%)
 Frame = +2

Query: 134 SLFVHRDTPEDNPSIPFEFTAPNQKRVEALLAIYPEGHKRGAMIPLLDLAQRXNGGWLPI 313
           +L  H D+P++ P +P+EF+  NQ +V+ +L+ YP  +K+ A+IPLLDLAQ+ NGGWLP+
Sbjct: 31  ALNYHLDSPDNKPDLPWEFSEANQSKVKEILSYYPSNYKQSAVIPLLDLAQQQNGGWLPV 90

Query: 314 SAMHKVAEILNLPRMRVYEVATFYTMFIRRPIGKYHVQVCTTTPCWLRGS 463
           SAM+ VA+++ +  +RVYEVATFY+MF R  +GKYH+ VC TTPC +RGS
Sbjct: 91  SAMNAVAKVIEVAPIRVYEVATFYSMFNRAKVGKYHLLVCGTTPCMIRGS 140


>At5g22330.1 68418.m02605 TATA box-binding protein-interacting
           protein-related similar to TATA box-binding
           protein-interacting protein SP:O35753 from [ Mus
           musculus]
          Length = 458

 Score = 31.9 bits (69), Expect = 0.22
 Identities = 15/30 (50%), Positives = 22/30 (73%)
 Frame = -3

Query: 154 VSVDEETVMLEGRSGLQTSRRHAPQGLNPS 65
           ++VDEE ++L G  G +TS RHA Q L+P+
Sbjct: 386 LTVDEECLVLLGEIGQRTSLRHAVQLLSPA 415


>At1g01450.1 68414.m00060 protein kinase-related contains eukaryotic
           protein kinase domain, INTERPRO:IPR000719
          Length = 470

 Score = 28.7 bits (61), Expect = 2.1
 Identities = 17/62 (27%), Positives = 33/62 (53%), Gaps = 1/62 (1%)
 Frame = +3

Query: 42  K*CYLASKLGFRPCGACLLEVCRPLLPSSMTVSSSTETPLKTTL-VSRLNLQRLIKRELK 218
           K C+L  +L  +  G  + EVC P   +++++  + +  L+  L +  L+ +R+   EL 
Sbjct: 92  KECFLVMELMRKTLGMHIKEVCGPRKKNTLSLPVAVDLMLQIALGMEYLHSKRIYHGELN 151

Query: 219 PS 224
           PS
Sbjct: 152 PS 153


>At5g57200.1 68418.m07145 epsin N-terminal homology (ENTH)
           domain-containing protein / clathrin assembly
           protein-related low similarity to clathrin assembly
           protein AP180 [Xenopus laevis] GI:6492344; contains Pfam
           profile PF01417: ENTH domain
          Length = 591

 Score = 27.9 bits (59), Expect = 3.6
 Identities = 12/27 (44%), Positives = 14/27 (51%)
 Frame = +2

Query: 224 LAIYPEGHKRGAMIPLLDLAQRXNGGW 304
           LAIYP GH+  A    L L +    GW
Sbjct: 404 LAIYPPGHETSAPSNSLSLIEAGGSGW 430


>At1g08260.1 68414.m00911 DNA-directed DNA polymerase epsilon
            catalytic subunit, putative similar to SP|Q07864 DNA
            polymerase epsilon, catalytic subunit A (EC 2.7.7.7) (DNA
            polymerase II subunit A) {Homo sapiens}; contains Pfam
            profiles: PF03175 DNA polymerase type B, organellar and
            viral, PF00136 DNA polymerase family B, PF03104 DNA
            polymerase family B, exonuclease domain
          Length = 2271

 Score = 27.9 bits (59), Expect = 3.6
 Identities = 10/26 (38%), Positives = 13/26 (50%)
 Frame = -3

Query: 454  KPAWSRCTHLYMILSYWSPNKHSIEC 377
            +P WS   HL   +  W  N HS +C
Sbjct: 1922 EPKWSVARHLPEYIEVWRQNPHSNQC 1947


>At5g38070.1 68418.m04587 zinc finger (C3HC4-type RING finger)
           family protein contains InterPro Entry IPR001841
           Zn-finger, RING; contains PROSITE PS00190: Cytochrome c
           family heme-binding site signature
          Length = 259

 Score = 27.5 bits (58), Expect = 4.8
 Identities = 13/25 (52%), Positives = 19/25 (76%)
 Frame = -3

Query: 208 LLIRRCKFKRDTRVVFRGVSVDEET 134
           ++I+RC+ +RDTR+   G S DEET
Sbjct: 217 IVIQRCRQERDTRL--SGFSSDEET 239


>At4g25940.1 68417.m03731 epsin N-terminal homology (ENTH)
           domain-containing protein contains Pfam PF01417: ENTH
           domain. ENTH (Epsin N-terminal homology) domain; similar
           to  Chain B, Crystal Structure Of N-Terminal Domain Of
           Drosophila Ap180 (GP:13399617) [Drosophila
           melanogaster]; supporting cDNA
           gi|20465326|gb|AY096427.1|
          Length = 601

 Score = 27.1 bits (57), Expect = 6.3
 Identities = 11/27 (40%), Positives = 14/27 (51%)
 Frame = +2

Query: 224 LAIYPEGHKRGAMIPLLDLAQRXNGGW 304
           LAIYP GH+      +L L +    GW
Sbjct: 415 LAIYPPGHEAPGPSNILSLIETGGSGW 441


>At1g74710.2 68414.m08655 isochorismate synthase 1 (ICS1) /
           isochorismate mutase identical to GI:17223087 and
           GB:AF078080; contains Pfam profile PF00425: chorismate
           binding enzyme; contains TIGRfam profile TIGR00543:
           isochorismate synthases; identical to cDNA isochorismate
           synthase 1 precursor (ICS1) nuclear gene for plastid
           product GI:17223086
          Length = 622

 Score = 27.1 bits (57), Expect = 6.3
 Identities = 11/23 (47%), Positives = 15/23 (65%)
 Frame = -3

Query: 421 MILSYWSPNKHSIECCNFIHSHS 353
           M+L Y +PN +S+ CC F  S S
Sbjct: 565 MVLCYQNPNFYSLFCCCFCSSPS 587


>At1g02940.1 68414.m00261 glutathione S-transferase, putative
           similar to glutathione S-transferase GI:860955 from
           [Hyoscyamus muticus]
          Length = 256

 Score = 27.1 bits (57), Expect = 6.3
 Identities = 13/46 (28%), Positives = 21/46 (45%)
 Frame = +2

Query: 329 VAEILNLPRMRVYEVATFYTMFIRRPIGKYHVQVCTTTPCWLRGSD 466
           +A++ +LP ++         MF+ RP  +  V   T  P W R  D
Sbjct: 201 MADLYHLPNIQYLMDTHTKRMFVNRPSVRRWVAEITARPAWKRACD 246


>At4g22330.1 68417.m03228 alkaline phytoceramidase family / aPHC
           family contains Pfam profile: PF05875: alkaline
           phytoceramidase (aPHC)
          Length = 255

 Score = 26.6 bits (56), Expect = 8.4
 Identities = 9/22 (40%), Positives = 14/22 (63%)
 Frame = -3

Query: 418 ILSYWSPNKHSIECCNFIHSHS 353
           I S+W P   +IECC   +++S
Sbjct: 5   ISSFWGPVTSTIECCEMNYAYS 26


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,023,227
Number of Sequences: 28952
Number of extensions: 186657
Number of successful extensions: 494
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 482
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 494
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 791932800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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