Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0006_H01
(510 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g15290.1 68416.m01931 3-hydroxybutyryl-CoA dehydrogenase, put... 52 3e-07
At1g56000.1 68414.m06425 amine oxidase-related contains Pfam pro... 31 0.34
At1g55980.1 68414.m06421 expressed protein 31 0.34
At4g29120.1 68417.m04168 6-phosphogluconate dehydrogenase NAD-bi... 30 0.79
At1g50080.1 68414.m05618 hypothetical protein 28 3.2
At5g26250.1 68418.m03131 sugar transporter, putative similar to ... 27 7.3
At3g52480.1 68416.m05771 expressed protein 27 7.3
At5g17700.1 68418.m02074 MATE efflux family protein similar to r... 27 9.7
At3g46340.1 68416.m05018 leucine-rich repeat protein kinase, put... 27 9.7
At2g27280.1 68415.m03278 hypothetical protein 27 9.7
At1g64320.1 68414.m07289 myosin heavy chain-related similar to m... 27 9.7
At1g56510.1 68414.m06498 disease resistance protein (TIR-NBS-LRR... 27 9.7
>At3g15290.1 68416.m01931 3-hydroxybutyryl-CoA dehydrogenase,
putative similar to S(+)-beta-hydroxybutyryl CoA
dehydrogenase (3-hydroxybutyryl-CoA dehydrogenase)
[Paracoccus denitrificans] GI:12003356; contains Pfam
profiles PF02737: 3-hydroxyacyl-CoA dehydrogenase NAD
binding, PF00725: 3-hydroxyacyl-CoA dehydrogenase
C-terminal
Length = 294
Score = 51.6 bits (118), Expect = 3e-07
Identities = 36/136 (26%), Positives = 61/136 (44%)
Frame = +1
Query: 46 EKVGIVGSGLIGRSWAMLFASVGYQVTLFDVVEKQITDAIADIKVQLKTLEKDGLLRGNL 225
+ VG+VG+G +G A L A+ G V L D ++ A A I +K GL+ +
Sbjct: 5 KSVGVVGAGQMGSGIAQLAATSGLDVWLMDADRDALSRATAAISSSVKRFVSKGLISKEV 64
Query: 226 NADEQFQCVKGTCDLAIAVKDAIFVQECVPENLELKKKVFQNLDNVVDDNTIXXXXXXXX 405
D+ ++ T +L I V E + E+ ++KKK+F++LD + + I
Sbjct: 65 -GDDAMHRLRLTSNLEDLCSADIIV-EAIVESEDIKKKLFKDLDGIAKSSAILASNTSSI 122
Query: 406 XXXXXXENMKH*AQVI 453
+ +QVI
Sbjct: 123 SITRLASATRRPSQVI 138
>At1g56000.1 68414.m06425 amine oxidase-related contains Pfam
profile PF01593: amine oxidase, flavin-containing
Length = 384
Score = 31.5 bits (68), Expect = 0.34
Identities = 16/41 (39%), Positives = 23/41 (56%)
Frame = +1
Query: 13 LRVVMASKFKSEKVGIVGSGLIGRSWAMLFASVGYQVTLFD 135
+R M+S + KV ++GSG+ G A A G VT+FD
Sbjct: 10 IRKQMSSVAVAMKVAVIGSGISGAVCASTLARNGVSVTIFD 50
>At1g55980.1 68414.m06421 expressed protein
Length = 464
Score = 31.5 bits (68), Expect = 0.34
Identities = 16/41 (39%), Positives = 23/41 (56%)
Frame = +1
Query: 13 LRVVMASKFKSEKVGIVGSGLIGRSWAMLFASVGYQVTLFD 135
+R M+S + KV ++GSG+ G A A G VT+FD
Sbjct: 148 IRKQMSSVAVAMKVAVIGSGISGAVCASTLARNGVSVTIFD 188
>At4g29120.1 68417.m04168 6-phosphogluconate dehydrogenase
NAD-binding domain-containing protein similar to
SP|P23523 2-hydroxy-3-oxopropionate reductase (EC
1.1.1.60) (Tartronate semialdehyde reductase)
{Escherichia coli}; contains Pfam profile PF03446: NAD
binding domain of 6-phosphogluconate dehydrogenase
Length = 334
Score = 30.3 bits (65), Expect = 0.79
Identities = 12/29 (41%), Positives = 19/29 (65%)
Frame = +1
Query: 49 KVGIVGSGLIGRSWAMLFASVGYQVTLFD 135
K+G +G+G++GRS GY VT+F+
Sbjct: 39 KIGWIGTGVMGRSMCGHLIKAGYTVTVFN 67
>At1g50080.1 68414.m05618 hypothetical protein
Length = 91
Score = 28.3 bits (60), Expect = 3.2
Identities = 16/57 (28%), Positives = 24/57 (42%)
Frame = -2
Query: 188 FNCTLISAIASVICFSTTSNKVTWYPTEANNIAHDLPISPLPTIPTFSDLNLEAMTT 18
FNC L+ AS I + + + NN+ H+ P PTI D + + T
Sbjct: 18 FNCVLLCMFASFIRKLAAQSTIYNLWKQRNNVVHNQVSIPAPTIFKLIDREIRNIIT 74
>At5g26250.1 68418.m03131 sugar transporter, putative similar to
hexose transporter [Lycopersicon esculentum] GI:5734440,
sugar carrier protein {Ricinus communis} SP|Q41144,
monosaccharide transporter [Nicotiana tabacum] GI:19885;
contains Pfam profile PF00083: major facilitator
superfamily protein
Length = 507
Score = 27.1 bits (57), Expect = 7.3
Identities = 13/34 (38%), Positives = 22/34 (64%)
Frame = +1
Query: 187 KTLEKDGLLRGNLNADEQFQCVKGTCDLAIAVKD 288
+TL+K +RG + DE+++ + CD+A VKD
Sbjct: 237 ETLKK---IRGVEDVDEEYESIVHACDIARQVKD 267
>At3g52480.1 68416.m05771 expressed protein
Length = 209
Score = 27.1 bits (57), Expect = 7.3
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = -2
Query: 281 TAIAKSQVPFTHWNCSSAFRFPLRSPSF 198
T I+ +V F+H+N ++ F P SP F
Sbjct: 159 TDISDDEVDFSHYNQTTPFSTPCASPPF 186
>At5g17700.1 68418.m02074 MATE efflux family protein similar to
ripening regulated protein DDTFR18 [Lycopersicon
esculentum] GI:12231296; contains Pfam profile PF01554:
Uncharacterized membrane protein family
Length = 497
Score = 26.6 bits (56), Expect = 9.7
Identities = 15/48 (31%), Positives = 27/48 (56%)
Frame = +1
Query: 43 SEKVGIVGSGLIGRSWAMLFASVGYQVTLFDVVEKQITDAIADIKVQL 186
S KV +V S +IG + L + G Q++ + ++DA+AD+ + L
Sbjct: 336 SIKVVLVVSAVIGVICSALCLAFGGQISYLFSDSQAVSDAVADLSIVL 383
>At3g46340.1 68416.m05018 leucine-rich repeat protein kinase,
putative similar to light repressible receptor protein
kinase [Arabidopsis thaliana] gi|1321686|emb|CAA66376;
contains leucine rich repeat (LRR) domains,
Pfam:PF00560; contains protein kinase domain,
Pfam:PF00069
Length = 889
Score = 26.6 bits (56), Expect = 9.7
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = +1
Query: 196 EKDGLLRGNLNADEQFQCVKGTC 264
EKDGL L+ DEQ +C G+C
Sbjct: 485 EKDGL---KLSVDEQIRCFPGSC 504
>At2g27280.1 68415.m03278 hypothetical protein
Length = 427
Score = 26.6 bits (56), Expect = 9.7
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = +1
Query: 133 DVVEKQITDAIADIKVQLKTLEK 201
D EK+ITDA+ K +L+ EK
Sbjct: 41 DTAEKEITDAVTTAKERLRETEK 63
>At1g64320.1 68414.m07289 myosin heavy chain-related similar to
myosin heavy chain (GI:4249703) [Rana catesbeiana];
similar to smooth muscle myosin heavy chain SM2
(GI:2352945) [Homo sapiens]
Length = 476
Score = 26.6 bits (56), Expect = 9.7
Identities = 24/65 (36%), Positives = 31/65 (47%), Gaps = 10/65 (15%)
Frame = +1
Query: 121 VTLFDVVEKQITDAIADIKVQLKTLEKD-GLLR-------GNLNAD--EQFQCVKGTCDL 270
V F E +T I D+K QLK LE++ G LR GNL E+ + VKG D
Sbjct: 38 VNKFGNSELGLTSRIEDLKCQLKNLEQEIGFLRARNAGLAGNLEVTKVEEKERVKGLMDQ 97
Query: 271 AIAVK 285
+K
Sbjct: 98 VNGMK 102
>At1g56510.1 68414.m06498 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1007
Score = 26.6 bits (56), Expect = 9.7
Identities = 15/61 (24%), Positives = 26/61 (42%), Gaps = 2/61 (3%)
Frame = -2
Query: 392 ELDKIVLSSTTLSKFWNTFFLSSKFSGTHSCTKIASLTAIAK--SQVPFTHWNCSSAFRF 219
E+D++++S K N FL +G H+ + + K + HW+ S R
Sbjct: 535 EIDELMISPKAFEKMCNLLFLKVYDAGWHTGKRKLDIPEDIKFPRTIRLFHWDAYSGKRL 594
Query: 218 P 216
P
Sbjct: 595 P 595
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,013,291
Number of Sequences: 28952
Number of extensions: 219520
Number of successful extensions: 697
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 675
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 696
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 917929344
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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