Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0006_G19
(491 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g29880.1 68415.m03628 hypothetical protein 33 0.14
At5g57560.1 68418.m07191 xyloglucan:xyloglucosyl transferase / x... 31 0.42
At5g50970.1 68418.m06321 WD-40 repeat family protein contains Pf... 30 0.97
At5g25500.1 68418.m03034 expressed protein ; expression supporte... 30 0.97
At3g18710.1 68416.m02376 U-box domain-containing protein similar... 28 3.0
At1g68960.1 68414.m07893 hypothetical protein contains Pfam prof... 28 3.0
At5g40590.1 68418.m04926 DC1 domain-containing protein predicted... 27 5.2
At4g19170.1 68417.m02829 9-cis-epoxycarotenoid dioxygenase, puta... 27 5.2
At1g59900.1 68414.m06748 pyruvate dehydrogenase E1 component alp... 27 5.2
At5g36140.1 68418.m04355 cytochrome P450-related similar to taxa... 27 6.9
At2g17975.1 68415.m02088 zinc finger (Ran-binding) family protei... 27 6.9
At5g53570.1 68418.m06656 RabGAP/TBC domain-containing protein si... 27 9.1
At5g48070.1 68418.m05939 xyloglucan:xyloglucosyl transferase, pu... 27 9.1
At3g24220.1 68416.m03039 9-cis-epoxycarotenoid dioxygenase, puta... 27 9.1
At2g44380.1 68415.m05520 DC1 domain-containing protein highly si... 27 9.1
>At2g29880.1 68415.m03628 hypothetical protein
Length = 308
Score = 32.7 bits (71), Expect = 0.14
Identities = 20/77 (25%), Positives = 38/77 (49%)
Frame = +3
Query: 141 ERFHRQLKAAIMCHSDQNWYEVIPLILLGIRNAWKEDIGSSSAELVYGETLKLPSDFFLA 320
E ++ K M SDQ YE+ +++ I+ W++ G+ S V + L L + F
Sbjct: 9 ETSKKKKKGPYMSWSDQECYELTAILVDAIKRGWRDKNGTISKTTVERKILPLLNKKFKC 68
Query: 321 SSVAEVTDYSDFLSRLR 371
+ Y+++LSR++
Sbjct: 69 NKT-----YTNYLSRMK 80
>At5g57560.1 68418.m07191 xyloglucan:xyloglucosyl transferase /
xyloglucan endotransglycosylase / endo-xyloglucan
transferase (TCH4) identical to xyloglucan
endotransglycosylase TCH4 protein GI:886116
Length = 284
Score = 31.1 bits (67), Expect = 0.42
Identities = 11/29 (37%), Positives = 14/29 (48%)
Frame = +2
Query: 212 INTSWDKKRLERRYRKFLSRACIWRNFKA 298
+ T W K YR F AC+W N K+
Sbjct: 198 VKTDWSKAPFTASYRGFQQEACVWSNGKS 226
>At5g50970.1 68418.m06321 WD-40 repeat family protein contains Pfam
profile PF00400: WD domain, G-beta repeat
Length = 512
Score = 29.9 bits (64), Expect = 0.97
Identities = 23/84 (27%), Positives = 39/84 (46%), Gaps = 2/84 (2%)
Frame = +3
Query: 33 DRG--RQFESYLFKELSKLTGSRHISTTAYHPAANGLVERFHRQLKAAIMCHSDQNWYEV 206
DRG R+FE KEL +L TT +N + + R+L++ I+C ++ +
Sbjct: 155 DRGIKRKFEQKEHKELIRLIARNSSPTTIKCHTSNQISSQHKRKLRSLILCPVNEQLFAT 214
Query: 207 IPLILLGIRNAWKEDIGSSSAELV 278
L G+ + W+ G A L+
Sbjct: 215 SSLD--GMVSLWQLQPGRLVASLL 236
>At5g25500.1 68418.m03034 expressed protein ; expression supported
by MPSS
Length = 420
Score = 29.9 bits (64), Expect = 0.97
Identities = 17/60 (28%), Positives = 33/60 (55%)
Frame = +3
Query: 297 LPSDFFLASSVAEVTDYSDFLSRLRRYMCNLKPTTVIRHGKSNIFVHKDLQKSSQVFLRN 476
+P+ + A++ A+ D D ++ LRRY+ + + TV+ H + + + L + VFL N
Sbjct: 228 VPARYAKANASAKWEDVLDMVNDLRRYLEHDEEITVLYHLDKLVSMKEGLLQIKDVFLDN 287
>At3g18710.1 68416.m02376 U-box domain-containing protein similar to
immediate-early fungal elicitor protein CMPG1
[Petroselinum crispum] GI:14582200; contains Pfam
profile PF04564: U-box domain
Length = 415
Score = 28.3 bits (60), Expect = 3.0
Identities = 10/19 (52%), Positives = 16/19 (84%)
Frame = +1
Query: 100 FLPRLTIQQLTDLWNVSIG 156
F+P LT+Q+L ++W+ SIG
Sbjct: 65 FVPNLTLQRLINIWSDSIG 83
>At1g68960.1 68414.m07893 hypothetical protein contains Pfam profile
PF03478: Protein of unknown function (DUF295)
Length = 376
Score = 28.3 bits (60), Expect = 3.0
Identities = 15/45 (33%), Positives = 23/45 (51%)
Frame = +1
Query: 58 IYLKNCLN*QVPVIFLPRLTIQQLTDLWNVSIGN*KQQLCVTPIK 192
+ L N LN +I LP LT + NVS+ + ++ CV +K
Sbjct: 102 VRLTNILNPSKKIISLPSLTRDKYESHVNVSVSSSNEEDCVVAVK 146
>At5g40590.1 68418.m04926 DC1 domain-containing protein predicted
protein, Arabidopsis thaliana
Length = 234
Score = 27.5 bits (58), Expect = 5.2
Identities = 10/15 (66%), Positives = 11/15 (73%)
Frame = -1
Query: 176 HNCCFQLPMETFHKS 132
H CF+LP ET HKS
Sbjct: 54 HKSCFELPRETNHKS 68
>At4g19170.1 68417.m02829 9-cis-epoxycarotenoid dioxygenase,
putative / neoxanthin cleavage enzyme, putative /
carotenoid cleavage dioxygenase, putative similar to
9-cis-epoxycarotenoid dioxygenase [Phaseolus
vulgaris][GI:6715257]; neoxanthin cleavage enzyme,
Lycopersicon esculentum, PATX:E325797
Length = 595
Score = 27.5 bits (58), Expect = 5.2
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +3
Query: 183 SDQNWYEVIPLILLGIRNAWKEDIGSS 263
S+ W+EV ++ NAW ED G+S
Sbjct: 390 SEMKWFEVPGFNIIHAINAWDEDDGNS 416
>At1g59900.1 68414.m06748 pyruvate dehydrogenase E1 component alpha
subunit, mitochondrial (PDHE1-A) identical to SP|P52901
Pyruvate dehydrogenase E1 component alpha subunit,
mitochondrial precursor (EC 1.2.4.1) (PDHE1-A)
{Arabidopsis thaliana}
Length = 389
Score = 27.5 bits (58), Expect = 5.2
Identities = 18/59 (30%), Positives = 27/59 (45%), Gaps = 4/59 (6%)
Frame = +2
Query: 95 SYFYHGL----PSSS*RTCGTFPSATESSNYVSLRSKLV*SHSINTSWDKKRLERRYRK 259
+Y YHG P S+ RT + + + KLV SH + T + K +E+ RK
Sbjct: 285 TYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERIKKLVLSHDLATEKELKDMEKEIRK 343
>At5g36140.1 68418.m04355 cytochrome P450-related similar to taxane
13-alpha-hydroxylase [Taxus cuspidata] GI:17148242
Length = 318
Score = 27.1 bits (57), Expect = 6.9
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = +3
Query: 3 RFGCPQKITTDRGRQFESYLFK 68
R G P+K TDR R F S +FK
Sbjct: 54 RQGHPEKFVTDRVRHFSSGIFK 75
>At2g17975.1 68415.m02088 zinc finger (Ran-binding) family protein
contains Pfam domain, PF00641: Zn-finger in Ran binding
protein and others
Length = 268
Score = 27.1 bits (57), Expect = 6.9
Identities = 11/39 (28%), Positives = 23/39 (58%)
Frame = +2
Query: 176 VSLRSKLV*SHSINTSWDKKRLERRYRKFLSRACIWRNF 292
++L +K + S ++ WD KRL + Y +++ I+ +F
Sbjct: 155 LALGTKRLASEALAHEWDSKRLNQGYTSMQTQSAIYASF 193
>At5g53570.1 68418.m06656 RabGAP/TBC domain-containing protein
similar to GTPase activating protein [Yarrowia
lipolytica] GI:2370595; contains Pfam profile PF00566:
TBC domain
Length = 550
Score = 26.6 bits (56), Expect = 9.1
Identities = 25/77 (32%), Positives = 37/77 (48%), Gaps = 7/77 (9%)
Frame = +2
Query: 92 PSYFYHGLPSSS*RTCGTFPSATESSNYVSLRSKL----V*SHSINTSWDKKR---LERR 250
PS PSS+ + S++ SS+++ LRS L + S S TS D++R RR
Sbjct: 31 PSSSSSSPPSSNSNSYSNSNSSSSSSSWIHLRSVLFVANLSSPSSVTSSDRRRKSPWSRR 90
Query: 251 YRKFLSRACIWRNFKAP 301
RK+ WR+ P
Sbjct: 91 KRKWALTPHQWRSLFTP 107
>At5g48070.1 68418.m05939 xyloglucan:xyloglucosyl transferase,
putative / xyloglucan endotransglycosylase, putative /
endo-xyloglucan transferase, putative similar to
xyloglucan endotransglycosylase TCH4 GI:886116 from
[Arabidopsis thaliana]
Length = 282
Score = 26.6 bits (56), Expect = 9.1
Identities = 10/27 (37%), Positives = 13/27 (48%)
Frame = +2
Query: 218 TSWDKKRLERRYRKFLSRACIWRNFKA 298
T W K YR + AC+W N K+
Sbjct: 207 TDWSKAPFTAFYRNYNVDACVWSNGKS 233
>At3g24220.1 68416.m03039 9-cis-epoxycarotenoid dioxygenase,
putative / neoxanthin cleavage enzyme, putative /
carotenoid cleavage dioxygenase, putative similar to
GB:CAB10168 from [Lycopersicon esculentum] (J. Exp. Bot.
47, 2111-2112 (1997)); similar to 9-cis-epoxycarotenoid
dioxygenase [Phaseolus vulgaris][GI:6715257]
Length = 577
Score = 26.6 bits (56), Expect = 9.1
Identities = 18/71 (25%), Positives = 26/71 (36%), Gaps = 1/71 (1%)
Frame = +3
Query: 183 SDQNWYEVIPLILLGIRNAWKEDIGSSSAEL-VYGETLKLPSDFFLASSVAEVTDYSDFL 359
SD NW +V + NAW+E + V G + P F S + S+
Sbjct: 376 SDINWVDVPDCFCFHLWNAWEERTEEGDPVIVVIGSCMSPPDTIFSESGEPTRVELSEIR 435
Query: 360 SRLRRYMCNLK 392
+R N K
Sbjct: 436 LNMRTKESNRK 446
>At2g44380.1 68415.m05520 DC1 domain-containing protein highly
similar to GP|2435515|AF024504; contains Pfam profile
PF03107: DC1 domain
Length = 247
Score = 26.6 bits (56), Expect = 9.1
Identities = 10/15 (66%), Positives = 10/15 (66%)
Frame = -1
Query: 176 HNCCFQLPMETFHKS 132
H CF LP ET HKS
Sbjct: 58 HKSCFDLPRETNHKS 72
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,249,104
Number of Sequences: 28952
Number of extensions: 199634
Number of successful extensions: 488
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 486
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 488
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 858708096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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