Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0006_E03
(557 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g58340.1 68414.m06636 MATE efflux protein-related contains Pf... 32 0.23
At4g29140.1 68417.m04170 MATE efflux protein-related several hyp... 31 0.39
At3g51550.1 68416.m05645 protein kinase family protein contains ... 31 0.39
At4g23030.1 68417.m03321 MATE efflux protein-related contains Pf... 30 0.91
At5g19700.1 68418.m02343 MATE efflux protein-related contains Pf... 29 1.6
At3g62360.1 68416.m07005 expressed protein 29 2.1
At4g09430.1 68417.m01553 disease resistance protein (TIR-NBS-LRR... 28 3.7
At2g38510.1 68415.m04732 MATE efflux protein-related contains Pf... 28 4.9
At3g18380.2 68416.m02338 expressed protein 27 6.4
At3g18380.1 68416.m02337 expressed protein 27 6.4
At5g47000.1 68418.m05793 peroxidase, putative 27 8.5
At3g61240.2 68416.m06854 DEAD/DEAH box helicase, putative (RH12)... 27 8.5
At3g61240.1 68416.m06853 DEAD/DEAH box helicase, putative (RH12)... 27 8.5
At3g46810.1 68416.m05081 DC1 domain-containing protein contains ... 27 8.5
>At1g58340.1 68414.m06636 MATE efflux protein-related contains Pfam
profile: PF01554 uncharacterized membrane protein family
UPF0013
Length = 532
Score = 32.3 bits (70), Expect = 0.23
Identities = 13/31 (41%), Positives = 19/31 (61%)
Frame = -1
Query: 419 WYWYEVSAVNCGLGSDCRATVPGLTLEISYT 327
W+WYE + CGL ++ RATV + + I T
Sbjct: 298 WWWYEFMIILCGLLANPRATVASMGILIQTT 328
>At4g29140.1 68417.m04170 MATE efflux protein-related several
hypothetical proteins - Arabidopsis thaliana; contains
Pfam profile PF01554: Uncharacterized membrane protein
family
Length = 532
Score = 31.5 bits (68), Expect = 0.39
Identities = 13/31 (41%), Positives = 19/31 (61%)
Frame = -1
Query: 419 WYWYEVSAVNCGLGSDCRATVPGLTLEISYT 327
W+WYE+ V CGL + R+TV + + I T
Sbjct: 296 WWWYEIMIVLCGLLVNPRSTVAAMGVLIQTT 326
>At3g51550.1 68416.m05645 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 895
Score = 31.5 bits (68), Expect = 0.39
Identities = 14/53 (26%), Positives = 26/53 (49%)
Frame = +1
Query: 265 AVNAESVDKPAFQESSFASVPVYDISNVRPGTVALQSLPSPQLTADTSYQYQP 423
A++ V+KP + +S V ++ ++ +P PQ+TAD S +P
Sbjct: 385 ALHPNPVNKPEYYDSLLNGVEIFKMNTSDGNLAGTNPIPGPQVTADPSKVLRP 437
>At4g23030.1 68417.m03321 MATE efflux protein-related contains Pfam
profile PF01554: Uncharacterized membrane protein family
Length = 502
Score = 30.3 bits (65), Expect = 0.91
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = -1
Query: 419 WYWYEVSAVNCGLGSDCRATVPGLTLEISYT 327
W+WYE+ + CGL + +ATV + + I T
Sbjct: 273 WWWYEIMILLCGLLLNPQATVASMGILIQTT 303
>At5g19700.1 68418.m02343 MATE efflux protein-related contains Pfam
profile PF01554: Uncharacterized membrane protein family
Length = 508
Score = 29.5 bits (63), Expect = 1.6
Identities = 13/36 (36%), Positives = 18/36 (50%)
Frame = -1
Query: 434 GIDNGWYWYEVSAVNCGLGSDCRATVPGLTLEISYT 327
G+ W+WYE+ V CGL D V + + I T
Sbjct: 271 GVCLEWWWYEIMTVLCGLLIDPSTPVASMGILIQTT 306
>At3g62360.1 68416.m07005 expressed protein
Length = 1227
Score = 29.1 bits (62), Expect = 2.1
Identities = 22/82 (26%), Positives = 36/82 (43%), Gaps = 4/82 (4%)
Frame = +1
Query: 256 ETNAVNAES-VDKPAFQESSFA---SVPVYDISNVRPGTVALQSLPSPQLTADTSYQYQP 423
ET+ ++A S V P + + +A S P Y I + P + + Q L + ++ +
Sbjct: 839 ETSTLSAGSFVAGPLYDDIPYATEASKPGYHIKRLGPYSFSCQKLGQISVRVNSKDNAET 898
Query: 424 LSIPAYNRFGGDPSYSTNSVSG 489
P GD Y NS+SG
Sbjct: 899 SIPPLLLSLSGDHGYRNNSISG 920
>At4g09430.1 68417.m01553 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1039
Score = 28.3 bits (60), Expect = 3.7
Identities = 17/64 (26%), Positives = 29/64 (45%), Gaps = 2/64 (3%)
Frame = -2
Query: 322 PMQTKILEKP--VYQQIPRSLHSFHLVSLLPKTSEV*QIHTSELTLKLSFQDCFPYSCLR 149
P + K L KP +Y + R++ H+V + K + + + LKL D +P L
Sbjct: 499 PSEQKFLWKPEEIYDVLARNIFLKHVVDITSKLQLISDVSSITHGLKLLHWDAYPLETLP 558
Query: 148 REYQ 137
+Q
Sbjct: 559 FSFQ 562
>At2g38510.1 68415.m04732 MATE efflux protein-related contains Pfam
profile PF01554: Uncharacterized membrane protein family
Length = 486
Score = 27.9 bits (59), Expect = 4.9
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = -1
Query: 419 WYWYEVSAVNCGLGSDCRATVPGLTLEISYTG 324
++WYE+ CGL + +A+V + + I TG
Sbjct: 247 YWWYEIMLFLCGLLGNPKASVAAMGILIQTTG 278
>At3g18380.2 68416.m02338 expressed protein
Length = 349
Score = 27.5 bits (58), Expect = 6.4
Identities = 20/64 (31%), Positives = 28/64 (43%), Gaps = 6/64 (9%)
Frame = +1
Query: 343 NVRPGTVALQSLPSPQLTADTSYQYQPLSIPAYNRFGGD-----PSYSTNSVSG-FKCGS 504
N PG + + S+P L QPLS+P G+ P+ S + V G + GS
Sbjct: 79 NKAPGKLNVSSMPRMDLPNQMRSVIQPLSVPKTTHMTGNLPGMTPAPSGSLVPGVMRSGS 138
Query: 505 DEFY 516
D Y
Sbjct: 139 DNSY 142
>At3g18380.1 68416.m02337 expressed protein
Length = 348
Score = 27.5 bits (58), Expect = 6.4
Identities = 20/64 (31%), Positives = 28/64 (43%), Gaps = 6/64 (9%)
Frame = +1
Query: 343 NVRPGTVALQSLPSPQLTADTSYQYQPLSIPAYNRFGGD-----PSYSTNSVSG-FKCGS 504
N PG + + S+P L QPLS+P G+ P+ S + V G + GS
Sbjct: 79 NKAPGKLNVSSMPRMDLPNQMRSVIQPLSVPKTTHMTGNLPGMTPAPSGSLVPGVMRSGS 138
Query: 505 DEFY 516
D Y
Sbjct: 139 DNSY 142
>At5g47000.1 68418.m05793 peroxidase, putative
Length = 334
Score = 27.1 bits (57), Expect = 8.5
Identities = 18/51 (35%), Positives = 23/51 (45%)
Frame = -2
Query: 307 ILEKPVYQQIPRSLHSFHLVSLLPKTSEV*QIHTSELTLKLSFQDCFPYSC 155
IL YQ+ H +V T +V Q T+ TL+L F DCF C
Sbjct: 32 ILRTDYYQKTCPDFHK--IVREAVTTKQVQQPTTAAGTLRLFFHDCFLEGC 80
>At3g61240.2 68416.m06854 DEAD/DEAH box helicase, putative (RH12)
identical to cDNA DEAD box RNA helicase, RH12 GI:3776000
Length = 498
Score = 27.1 bits (57), Expect = 8.5
Identities = 16/55 (29%), Positives = 28/55 (50%)
Frame = -2
Query: 187 LSFQDCFPYSCLRREYQRHLFRPIIVLLLDTPRDAPMGSFHVFLQERGPSVSCQN 23
L F FP + ++ RHL +P ++ L+D + ++ F++ER V C N
Sbjct: 301 LMFSATFPVT-VKAFKDRHLRKPYVINLMDQLTLMGVTQYYAFVEER-QKVHCLN 353
>At3g61240.1 68416.m06853 DEAD/DEAH box helicase, putative (RH12)
identical to cDNA DEAD box RNA helicase, RH12 GI:3776000
Length = 498
Score = 27.1 bits (57), Expect = 8.5
Identities = 16/55 (29%), Positives = 28/55 (50%)
Frame = -2
Query: 187 LSFQDCFPYSCLRREYQRHLFRPIIVLLLDTPRDAPMGSFHVFLQERGPSVSCQN 23
L F FP + ++ RHL +P ++ L+D + ++ F++ER V C N
Sbjct: 301 LMFSATFPVT-VKAFKDRHLRKPYVINLMDQLTLMGVTQYYAFVEER-QKVHCLN 353
>At3g46810.1 68416.m05081 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 686
Score = 27.1 bits (57), Expect = 8.5
Identities = 16/51 (31%), Positives = 28/51 (54%)
Frame = -2
Query: 379 GVIAELRFLV*RWKYHIQGPMQTKILEKPVYQQIPRSLHSFHLVSLLPKTS 227
GV + +V + YH+ GP+ T + E Y+ +P ++ S L ++P TS
Sbjct: 596 GVTLHVSCVVGDFSYHMPGPLSTVVSE---YKMVPPAVVS--LYKVVPNTS 641
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,166,965
Number of Sequences: 28952
Number of extensions: 248670
Number of successful extensions: 660
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 645
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 659
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1062855648
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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