Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0006_C16
(582 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g78570.1 68414.m09157 NAD-dependent epimerase/dehydratase fam... 42 2e-04
At1g53500.1 68414.m06066 NAD-dependent epimerase/dehydratase fam... 38 0.005
At2g33630.1 68415.m04123 3-beta hydroxysteroid dehydrogenase/iso... 38 0.006
At3g14790.1 68416.m01869 NAD-dependent epimerase/dehydratase fam... 37 0.011
At3g23820.1 68416.m02994 NAD-dependent epimerase/dehydratase fam... 32 0.32
At4g33360.1 68417.m04743 terpene cyclase/mutase-related low simi... 31 0.74
At2g26260.1 68415.m03152 3-beta hydroxysteroid dehydrogenase/iso... 31 0.74
At1g68540.1 68414.m07830 oxidoreductase family protein similar t... 30 0.98
At1g25460.1 68414.m03161 oxidoreductase family protein similar t... 30 1.3
At5g42800.1 68418.m05213 dihydroflavonol 4-reductase (dihydrokae... 29 2.3
At1g09510.1 68414.m01066 cinnamyl-alcohol dehydrogenase family /... 29 2.3
At3g57050.3 68416.m06352 cystathionine beta-lyase, chloroplast /... 28 4.0
At3g57050.2 68416.m06351 cystathionine beta-lyase, chloroplast /... 28 4.0
At3g57050.1 68416.m06350 cystathionine beta-lyase, chloroplast /... 28 4.0
At1g47290.2 68414.m05236 3-beta hydroxysteroid dehydrogenase/iso... 28 4.0
At1g47290.1 68414.m05235 3-beta hydroxysteroid dehydrogenase/iso... 28 4.0
At1g07745.1 68414.m00836 DNA repair family protein contains simi... 27 6.9
At1g66800.1 68414.m07593 cinnamyl-alcohol dehydrogenase family /... 27 9.1
>At1g78570.1 68414.m09157 NAD-dependent epimerase/dehydratase family
protein similar to dTDP-glucose 4,6-dehydratase from
Aneurinibacillus thermoaerophilus GI:16357461, RmlB from
Leptospira borgpetersenii GI:4234803; contains Pfam
profile PF01370 NAD dependent epimerase/dehydratase
family
Length = 669
Score = 42.3 bits (95), Expect = 2e-04
Identities = 42/147 (28%), Positives = 63/147 (42%), Gaps = 8/147 (5%)
Frame = +3
Query: 135 LSPKRHADSRVSTLAADVTAPGVAEQLVA--GADVLFHLAAVVSGHAEIDFDLGLRV--- 299
L+P +H+ + + D+ + + L+ G D + H AA H + F
Sbjct: 50 LNPSKHSPN-FKFVKGDIASADLVNHLLITEGIDTIMHFAAQT--HVDNSFGNSFEFTKN 106
Query: 300 NFDATRALLEAARRKAPNLRFVFASTVGVFGGEPPPA-VG--ELTVTAPHSSYGAAKAMS 470
N T LLEA + RF+ ST V+G A VG E + P + Y A KA +
Sbjct: 107 NIYGTHVLLEACKVTGQIRRFIHVSTDEVYGETDEDALVGNHEASQLLPTNPYSATKAGA 166
Query: 471 ELLVNDYARRGWADARAVRLPTVSVRG 551
E+LV Y R+ LP ++ RG
Sbjct: 167 EMLVMAY-------GRSYGLPVITTRG 186
>At1g53500.1 68414.m06066 NAD-dependent epimerase/dehydratase family
protein low similarity to dTDP-D-glucose-4,6-dehydratase
from Aneurinibacillus thermoaerophilus GI:16357461,
Sphingomonas sp. GI:1314581; contains Pfam profile
PF01370: NAD dependent epimerase/dehydratase family;
putative NDP-rhamnose synthase (rhm2 gene) GI:31559258
Length = 667
Score = 37.9 bits (84), Expect = 0.005
Identities = 36/114 (31%), Positives = 49/114 (42%), Gaps = 6/114 (5%)
Frame = +3
Query: 228 DVLFHLAAVVSGHAEIDFDLGLRV---NFDATRALLEAARRKAPNLRFVFASTVGVFG-G 395
D + H AA H + F N T LLEA + RF+ ST V+G
Sbjct: 84 DTIMHFAAQT--HVDNSFGNSFEFTKNNIYGTHVLLEACKVTGQIRRFIHVSTDEVYGET 141
Query: 396 EPPPAVG--ELTVTAPHSSYGAAKAMSELLVNDYARRGWADARAVRLPTVSVRG 551
+ AVG E + P + Y A KA +E+LV Y R+ LP ++ RG
Sbjct: 142 DEDAAVGNHEASQLLPTNPYSATKAGAEMLVMAY-------GRSYGLPVITTRG 188
>At2g33630.1 68415.m04123 3-beta hydroxysteroid
dehydrogenase/isomerase family protein contains Pfam
profile PF01073 3-beta hydroxysteroid
dehydrogenase/isomerase domain; similar to
NAD(P)-dependent cholesterol dehydrogenase [Nocardia
sp.] [GI:216809]
Length = 480
Score = 37.5 bits (83), Expect = 0.006
Identities = 30/83 (36%), Positives = 42/83 (50%), Gaps = 2/83 (2%)
Frame = +3
Query: 156 DSRVSTLAADVTAPGVAEQLVAGADVLFHLAAV-VSGHAEIDFDLGLRVNFDATRALLEA 332
+S V + DVT + + GAD + HLA+ +SG + F VN + T +LEA
Sbjct: 56 NSGVRCIQGDVTKKQDVDNALDGADCVLHLASYGMSGKEMLRFGRCDEVNINGTCNVLEA 115
Query: 333 ARRKAPNLRFVFASTVG-VFGGE 398
A K R V+ ST VFGG+
Sbjct: 116 A-FKHEITRIVYVSTYNVVFGGK 137
>At3g14790.1 68416.m01869 NAD-dependent epimerase/dehydratase family
protein similar to dTDP-glucose 4,6-dehydratase from
Aneurinibacillus thermoaerophilus GI:16357461,
Saccharopolyspora spinosa GI:15077647, RmlB from
Leptospira borgpetersenii GI:4234803; contains Pfam
profile PF01370 NAD dependent epimerase/dehydratase
family
Length = 664
Score = 36.7 bits (81), Expect = 0.011
Identities = 35/114 (30%), Positives = 49/114 (42%), Gaps = 6/114 (5%)
Frame = +3
Query: 228 DVLFHLAAVVSGHAEIDFDLGLRV---NFDATRALLEAARRKAPNLRFVFASTVGVFG-G 395
D + H AA H + F N T LLEA + RF+ ST V+G
Sbjct: 82 DTIMHFAAQT--HVDNSFGNSFEFTKNNIYGTHVLLEACKVTGQIRRFIHVSTDEVYGET 139
Query: 396 EPPPAVG--ELTVTAPHSSYGAAKAMSELLVNDYARRGWADARAVRLPTVSVRG 551
+ +VG E + P + Y A KA +E+LV Y R+ LP ++ RG
Sbjct: 140 DEDASVGNHEASQLLPTNPYSATKAGAEMLVMAY-------GRSYGLPVITTRG 186
>At3g23820.1 68416.m02994 NAD-dependent epimerase/dehydratase family
protein similar to nucleotide sugar epimerase from
Vibrio vulnificus GI:3093975 [PID:g3093975], WbnF
[Escherichia coli] GI:5739472, CAPI protein
{Staphylococcus aureus} SP|P39858; contains Pfam
profile: PF01370 NAD dependent epimerase/dehydratase
family
Length = 460
Score = 31.9 bits (69), Expect = 0.32
Identities = 26/88 (29%), Positives = 35/88 (39%), Gaps = 1/88 (1%)
Frame = +3
Query: 321 LLEAARRKAPNLRFVFASTVGVFG-GEPPPAVGELTVTAPHSSYGAAKAMSELLVNDYAR 497
LLE A+ P V+AS+ V+G P E P S Y A K E + + Y
Sbjct: 220 LLEVAKAANPQPAIVWASSSSVYGLNTENPFSEEHRTDQPASLYAATKKAGEEIAHTYNH 279
Query: 498 RGWADARAVRLPTVSVRGGAPNLAVTSF 581
+R TV G P++A F
Sbjct: 280 IYGLSLTGLRFFTVYGPWGRPDMAYFFF 307
>At4g33360.1 68417.m04743 terpene cyclase/mutase-related low
similarity to squalene-hopene cyclase from Zymomonas
mobilis [SP|P33990]
Length = 344
Score = 30.7 bits (66), Expect = 0.74
Identities = 37/156 (23%), Positives = 60/156 (38%), Gaps = 3/156 (1%)
Frame = +3
Query: 45 LGARLADYLLENECPLRVSALLLTDVHQPTLSPKRHADSRVSTLAADVTAPGVAEQLVAG 224
LGARL LL +R +D+ L P+ V DVT +G
Sbjct: 24 LGARLCHVLLRRGHSVRALVRRTSDLSD--LPPE------VELAYGDVTDYRSLTDACSG 75
Query: 225 ADVLFHLAAVVSGHAEIDFDLGLRVNFDATRALLEAARRKAPNLRFVFASTVGVFGGEPP 404
D++FH AA+V D + VN + +LEA + + ++ S+ G
Sbjct: 76 CDIVFHAAALVEPWLP-DPSRFISVNVGGLKNVLEAVKETKTVQKIIYTSSFFALGSTDG 134
Query: 405 PAVGELTVTAPH---SSYGAAKAMSELLVNDYARRG 503
E V + Y +KA+++ + + A G
Sbjct: 135 SVANENQVHNERFFCTEYERSKAVADKMALNAASEG 170
>At2g26260.1 68415.m03152 3-beta hydroxysteroid
dehydrogenase/isomerase family protein contains Pfam
profile PF01073 3-beta hydroxysteroid
dehydrogenase/isomerase domain; similar to
NAD(P)-dependent steroid dehydrogenase from Homo sapiens
[SP|Q15738], Mus musculus [SP|Q9R1J0]
Length = 564
Score = 30.7 bits (66), Expect = 0.74
Identities = 30/110 (27%), Positives = 48/110 (43%), Gaps = 3/110 (2%)
Frame = +3
Query: 162 RVSTLAADVTAPGVAEQLVAGADVLFHLAAVVSGHAEIDFDLGLRVNFDATRALLEAARR 341
RV ++AD+ + GA+V+FH+AA S + + L VN T+ +++A
Sbjct: 65 RVQYISADLRDKSQVVKAFQGAEVVFHMAAPDS--SINNHQLQYSVNVQGTQNVIDACVD 122
Query: 342 KAPNLRFVFASTVGVFGGEPPPAVGELTVTAP---HSSYGAAKAMSELLV 482
+S VF G G ++ P + SY A KA E L+
Sbjct: 123 VGVKRLIYTSSPSVVFDGVHGILNGTESMAYPIKHNDSYSATKAEGEELI 172
>At1g68540.1 68414.m07830 oxidoreductase family protein similar to
cinnamoyl CoA reductase [Eucalyptus gunnii, gi:2058311],
cinnamyl-alcohol dehydrogenase, E. gunnii [gi:1143445],
CPRD14 protein, Vigna unguiculata [gi:1854445]
Length = 321
Score = 30.3 bits (65), Expect = 0.98
Identities = 23/77 (29%), Positives = 34/77 (44%), Gaps = 2/77 (2%)
Frame = +3
Query: 153 ADSRVSTLAADVTAPGVAEQLVAGADVLFHLAA--VVSGHAEIDFDLGLRVNFDATRALL 326
A R+ L AD+T G ++ V G D +FH A+ +V I L + T +
Sbjct: 50 AKQRLKILQADLTVEGSFDEAVNGVDGVFHTASPVLVPQDHNIQETLVDPIIKGTTNVMS 109
Query: 327 EAARRKAPNLRFVFAST 377
A+ KA R V S+
Sbjct: 110 SCAKSKATLKRIVLTSS 126
>At1g25460.1 68414.m03161 oxidoreductase family protein similar to
dihydroflavonol 4-reductase GI:1332411 from [Rosa
hybrida], cinnamoyl CoA reductase from Pinus taeda
[gi:17978649], Eucalyptus gunnii [gi:2058311]
Length = 320
Score = 29.9 bits (64), Expect = 1.3
Identities = 14/36 (38%), Positives = 21/36 (58%)
Frame = +3
Query: 153 ADSRVSTLAADVTAPGVAEQLVAGADVLFHLAAVVS 260
A R+ AD+T G ++ V G D +FH+A+ VS
Sbjct: 50 AKERLKIFEADLTIEGSFDEAVNGVDGVFHIASRVS 85
>At5g42800.1 68418.m05213 dihydroflavonol 4-reductase
(dihydrokaempferol 4-reductase) (DFR) nearly identical
to GI:166686
Length = 382
Score = 29.1 bits (62), Expect = 2.3
Identities = 17/83 (20%), Positives = 39/83 (46%)
Frame = +3
Query: 150 HADSRVSTLAADVTAPGVAEQLVAGADVLFHLAAVVSGHAEIDFDLGLRVNFDATRALLE 329
+A + ++ AD++ G + + G D +FH+A + ++ + ++ + +++
Sbjct: 53 NAKTLLTLWKADLSEEGSYDDAINGCDGVFHVATPMDFESKDPENEVIKPTVNGMLGIMK 112
Query: 330 AARRKAPNLRFVFASTVGVFGGE 398
A + RFVF S+ G E
Sbjct: 113 ACVKAKTVRRFVFTSSAGTVNVE 135
>At1g09510.1 68414.m01066 cinnamyl-alcohol dehydrogenase family /
CAD family similar to cinnamyl alcohol dehydrogenase,
Eucalyptus gunnii [gi:1143445], CPRD14 protein, Vigna
unguiculata [gi:1854445]
Length = 322
Score = 29.1 bits (62), Expect = 2.3
Identities = 13/36 (36%), Positives = 19/36 (52%)
Frame = +3
Query: 153 ADSRVSTLAADVTAPGVAEQLVAGADVLFHLAAVVS 260
A ++ AD+ G EQ + G D +FH A+ VS
Sbjct: 54 AKEKLKLFKADLLEEGSFEQAIEGCDAVFHTASPVS 89
>At3g57050.3 68416.m06352 cystathionine beta-lyase, chloroplast /
beta-cystathionase / cysteine lyase (CBL) identical to
SP|P53780 Cystathionine beta-lyase, chloroplast
precursor (EC 4.4.1.8) (CBL) (Beta-cystathionase)
(Cysteine lyase) {Arabidopsis thaliana}
Length = 378
Score = 28.3 bits (60), Expect = 4.0
Identities = 12/48 (25%), Positives = 26/48 (54%), Gaps = 1/48 (2%)
Frame = +3
Query: 135 LSPKRHADSRVSTLAADVTAPGVAEQLVAGADVLFHLAA-VVSGHAEI 275
+S HA + + + +P ++ L GAD++ H A ++GH+++
Sbjct: 239 ISEMAHAQGALVLVDNSIMSPVLSRPLELGADIVMHSATKFIAGHSDV 286
>At3g57050.2 68416.m06351 cystathionine beta-lyase, chloroplast /
beta-cystathionase / cysteine lyase (CBL) identical to
SP|P53780 Cystathionine beta-lyase, chloroplast
precursor (EC 4.4.1.8) (CBL) (Beta-cystathionase)
(Cysteine lyase) {Arabidopsis thaliana}
Length = 449
Score = 28.3 bits (60), Expect = 4.0
Identities = 12/48 (25%), Positives = 26/48 (54%), Gaps = 1/48 (2%)
Frame = +3
Query: 135 LSPKRHADSRVSTLAADVTAPGVAEQLVAGADVLFHLAA-VVSGHAEI 275
+S HA + + + +P ++ L GAD++ H A ++GH+++
Sbjct: 224 ISEMAHAQGALVLVDNSIMSPVLSRPLELGADIVMHSATKFIAGHSDV 271
>At3g57050.1 68416.m06350 cystathionine beta-lyase, chloroplast /
beta-cystathionase / cysteine lyase (CBL) identical to
SP|P53780 Cystathionine beta-lyase, chloroplast
precursor (EC 4.4.1.8) (CBL) (Beta-cystathionase)
(Cysteine lyase) {Arabidopsis thaliana}
Length = 464
Score = 28.3 bits (60), Expect = 4.0
Identities = 12/48 (25%), Positives = 26/48 (54%), Gaps = 1/48 (2%)
Frame = +3
Query: 135 LSPKRHADSRVSTLAADVTAPGVAEQLVAGADVLFHLAA-VVSGHAEI 275
+S HA + + + +P ++ L GAD++ H A ++GH+++
Sbjct: 239 ISEMAHAQGALVLVDNSIMSPVLSRPLELGADIVMHSATKFIAGHSDV 286
>At1g47290.2 68414.m05236 3-beta hydroxysteroid
dehydrogenase/isomerase family protein contains Pfam
profile PF01073 3-beta hydroxysteroid
dehydrogenase/isomerase domain; similar to
NAD(P)-dependent steroid dehydrogenase from Homo sapiens
[SP|Q15738], Mus musculus [SP|Q9R1J0]
Length = 439
Score = 28.3 bits (60), Expect = 4.0
Identities = 29/110 (26%), Positives = 47/110 (42%), Gaps = 3/110 (2%)
Frame = +3
Query: 162 RVSTLAADVTAPGVAEQLVAGADVLFHLAAVVSGHAEIDFDLGLRVNFDATRALLEAARR 341
RV ++AD+ + GA+V+FH+AA S + + L VN T +++A
Sbjct: 65 RVQYVSADLRNKTQVVKGFQGAEVVFHMAAPDS--SINNHQLQYSVNVQGTTNVIDACIE 122
Query: 342 KAPNLRFVFASTVGVFGGEPPPAVGELTVTAP---HSSYGAAKAMSELLV 482
+S VF G + ++ P + SY A KA E L+
Sbjct: 123 VGVKRLIYTSSPSVVFDGVHGTLNADESLPYPPKHNDSYSATKAEGEALI 172
>At1g47290.1 68414.m05235 3-beta hydroxysteroid
dehydrogenase/isomerase family protein contains Pfam
profile PF01073 3-beta hydroxysteroid
dehydrogenase/isomerase domain; similar to
NAD(P)-dependent steroid dehydrogenase from Homo sapiens
[SP|Q15738], Mus musculus [SP|Q9R1J0]
Length = 382
Score = 28.3 bits (60), Expect = 4.0
Identities = 29/110 (26%), Positives = 47/110 (42%), Gaps = 3/110 (2%)
Frame = +3
Query: 162 RVSTLAADVTAPGVAEQLVAGADVLFHLAAVVSGHAEIDFDLGLRVNFDATRALLEAARR 341
RV ++AD+ + GA+V+FH+AA S + + L VN T +++A
Sbjct: 65 RVQYVSADLRNKTQVVKGFQGAEVVFHMAAPDS--SINNHQLQYSVNVQGTTNVIDACIE 122
Query: 342 KAPNLRFVFASTVGVFGGEPPPAVGELTVTAP---HSSYGAAKAMSELLV 482
+S VF G + ++ P + SY A KA E L+
Sbjct: 123 VGVKRLIYTSSPSVVFDGVHGTLNADESLPYPPKHNDSYSATKAEGEALI 172
>At1g07745.1 68414.m00836 DNA repair family protein contains
similarity to Swiss-Prot:O75771 DNA repair protein RAD51
homolog 4 (R51H3) (TRAD) [Homo sapiens]
Length = 200
Score = 27.5 bits (58), Expect = 6.9
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = +3
Query: 390 GGEPPPAVGELTVTAPHSSYGAAKAMS 470
GG+ PA+GE + PH S AAK ++
Sbjct: 166 GGKTKPALGETWKSIPHPSGQAAKTLT 192
>At1g66800.1 68414.m07593 cinnamyl-alcohol dehydrogenase family /
CAD family similar to cinnamyl alcohol dehydrogenase
[Eucalyptus gunnii] GI:1143445, CPRD14 protein, Vigna
unguiculata [gi:1854445]
Length = 319
Score = 27.1 bits (57), Expect = 9.1
Identities = 12/36 (33%), Positives = 19/36 (52%)
Frame = +3
Query: 153 ADSRVSTLAADVTAPGVAEQLVAGADVLFHLAAVVS 260
A R+ +D+ G +Q + G D +FH A+ VS
Sbjct: 54 ASERLKLFKSDLLEEGSFDQAIEGCDGVFHTASPVS 89
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,967,125
Number of Sequences: 28952
Number of extensions: 168074
Number of successful extensions: 667
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 644
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 665
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1141585696
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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