Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0006_C03
(437 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g35940.1 68418.m04325 jacalin lectin family protein similar t... 29 1.4
At5g14950.1 68418.m01754 glycosyl hydrolase family 38 protein si... 28 3.2
At3g25140.1 68416.m03139 glycosyl transferase family 8 protein c... 28 3.2
At2g20170.1 68415.m02358 hypothetical protein and grail contain... 28 3.2
At5g59920.1 68418.m07514 DC1 domain-containing protein contains ... 27 5.5
At3g13170.1 68416.m01647 DNA topoisomerase VIA (SPO11-1) identic... 27 5.5
At5g19010.1 68418.m02258 mitogen-activated protein kinase, putat... 26 9.7
At4g33840.1 68417.m04802 glycosyl hydrolase family 10 protein xy... 26 9.7
At4g00880.1 68417.m00119 auxin-responsive family protein similar... 26 9.7
At2g24540.1 68415.m02931 kelch repeat-containing F-box family pr... 26 9.7
At1g70900.1 68414.m08181 expressed protein 26 9.7
At1g23110.1 68414.m02889 hypothetical protein 26 9.7
At1g22420.1 68414.m02803 hydroxyproline-rich glycoprotein family... 26 9.7
>At5g35940.1 68418.m04325 jacalin lectin family protein similar to
myrosinase-binding protein homolog [Arabidopsis
thaliana] GI:2997767; contains Pfam profile PF01419
jacalin-like lectin domain
Length = 444
Score = 29.1 bits (62), Expect = 1.4
Identities = 18/61 (29%), Positives = 26/61 (42%), Gaps = 1/61 (1%)
Frame = +2
Query: 155 DKNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFSGNGDYSGVANPYISLSKT-FSEMNPD 331
DKNV S LG + +L +G + + GDY G+ Y++ T M D
Sbjct: 131 DKNVYS--LGAYFIKVLATKSGMQGGQNTGKSYDYGGDYDGIRKVYVTYDGTSIRHMRVD 188
Query: 332 Y 334
Y
Sbjct: 189 Y 189
>At5g14950.1 68418.m01754 glycosyl hydrolase family 38 protein
similar to alpha-mannosidase II SP:P27046 from [Mus
musculus]
Length = 1173
Score = 27.9 bits (59), Expect = 3.2
Identities = 10/29 (34%), Positives = 18/29 (62%)
Frame = -3
Query: 210 LYRSISIITPNGEDTTFLSFDDVYVSLRN 124
LYR+ +++ P G+D ++S D+ RN
Sbjct: 401 LYRTNTLLIPLGDDFRYISIDEAEAQFRN 429
>At3g25140.1 68416.m03139 glycosyl transferase family 8 protein
contains Pfam profile: PF01501 glycosyl transferase
family 8
Length = 559
Score = 27.9 bits (59), Expect = 3.2
Identities = 17/77 (22%), Positives = 35/77 (45%)
Frame = +2
Query: 161 NVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFSGNGDYSGVANPYISLSKTFSEMNPDYFT 340
+VV+ + + + +++K +G+ E+ DY+ + + Y+ + K N F
Sbjct: 281 HVVTDKMNLGAMQVMFKLKEYKGAHVEVKAVE---DYTFLNSSYVPVLKQLESANLQKFY 337
Query: 341 MANKIYVGHKYTLDEKF 391
NK+ K T + KF
Sbjct: 338 FENKLENATKDTTNMKF 354
>At2g20170.1 68415.m02358 hypothetical protein and grail contains
Pfam profile PF03080: Arabidopsis proteins of unknown
function
Length = 401
Score = 27.9 bits (59), Expect = 3.2
Identities = 19/71 (26%), Positives = 31/71 (43%)
Frame = +2
Query: 83 RTALGDAIDKTSLQFLKETYTSSKDKNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFSGN 262
RT L D I L++L YT+SKDK+ ++ + Y+ G + N
Sbjct: 121 RTTLEDLIQIQRLKYLGVKYTTSKDKDFLNMTGRHFAIAEYYRDNYGATGNINLWDPPVN 180
Query: 263 GDYSGVANPYI 295
D +A+ Y+
Sbjct: 181 PDQFSLASIYV 191
>At5g59920.1 68418.m07514 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 710
Score = 27.1 bits (57), Expect = 5.5
Identities = 15/41 (36%), Positives = 20/41 (48%)
Frame = +1
Query: 76 VQQDSLRRCH*QNFFAVS*GNVYIIEGQECCVFTIRRDDAN 198
V D RRC V G++Y+ E +ECC F + AN
Sbjct: 384 VIHDERRRCE-ACIHPVQFGSIYVCEEEECC-FVLHEKCAN 422
>At3g13170.1 68416.m01647 DNA topoisomerase VIA (SPO11-1) identical
to AtSPO11-1 [Arabidopsis thaliana] GI:13383478;
contains Pfam profile PF04406: Type IIB DNA
topoisomerase; identical to cDNA putative topoisomerase
VIA (SPO11 gene 1) GI:7270974
Length = 362
Score = 27.1 bits (57), Expect = 5.5
Identities = 13/27 (48%), Positives = 16/27 (59%)
Frame = +2
Query: 98 DAIDKTSLQFLKETYTSSKDKNVVSSP 178
+A+ SL FL E Y SK + VSSP
Sbjct: 336 EALSVHSLSFLSEVYIPSKIRREVSSP 362
>At5g19010.1 68418.m02258 mitogen-activated protein kinase, putative
/ MAPK, putative (MPK16) mitogen-activated protein
kinase (MAPK)(AtMPK16), PMID:12119167; similar to
ATMPK9, Arabidopsis thaliana, EMBL:AB038694
Length = 567
Score = 26.2 bits (55), Expect = 9.7
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = +2
Query: 221 GEGSRAEIDKFSGNGDYSGVANPY 292
GEGSR I++ G G Y V + Y
Sbjct: 20 GEGSRYRIEEVIGKGSYGVVCSAY 43
>At4g33840.1 68417.m04802 glycosyl hydrolase family 10 protein xylan
endohydrolase isoenzyme X-I, Hordeum
vulgare,PID:g1813595
Length = 576
Score = 26.2 bits (55), Expect = 9.7
Identities = 12/42 (28%), Positives = 20/42 (47%)
Frame = +2
Query: 53 SVYSSDHEYNRTALGDAIDKTSLQFLKETYTSSKDKNVVSSP 178
S + YN + A+D + F+ E T + K++ SSP
Sbjct: 350 SKFGPKASYNTYTMAHAVDPRTPMFMNEYNTLEQPKDLTSSP 391
>At4g00880.1 68417.m00119 auxin-responsive family protein similar to
small auxin up RNA (GI:546362) {Arabidopsis thaliana}
Length = 122
Score = 26.2 bits (55), Expect = 9.7
Identities = 9/22 (40%), Positives = 12/22 (54%)
Frame = -2
Query: 250 LVDLRTTTFTGASFVQKH*HHH 185
L+D T F G + + H HHH
Sbjct: 92 LIDRENTRFLGTNLLDHHHHHH 113
>At2g24540.1 68415.m02931 kelch repeat-containing F-box family
protein similar to SKP1 interacting partner 4
[Arabidopsis thaliana] GI:10716953; contains Pfam
profiles PF01344: Kelch motif, PF00646: F-box domain
Length = 372
Score = 26.2 bits (55), Expect = 9.7
Identities = 15/30 (50%), Positives = 19/30 (63%), Gaps = 2/30 (6%)
Frame = -3
Query: 276 PL*SPLPENLS--ISARLPSPAPALYRSIS 193
PL S LP +++ RLP P ALYRS+S
Sbjct: 27 PLISGLPNDIAELCLLRLPYPYHALYRSVS 56
>At1g70900.1 68414.m08181 expressed protein
Length = 244
Score = 26.2 bits (55), Expect = 9.7
Identities = 18/66 (27%), Positives = 31/66 (46%)
Frame = +2
Query: 158 KNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFSGNGDYSGVANPYISLSKTFSEMNPDYF 337
K +S +GV + LY + SR + K+ DY+ +A I L++ E NP +
Sbjct: 103 KVYANSLIGVGIASSLYHS-----SRGKFRKYLRWADYTMIATATICLTRALREENPKFL 157
Query: 338 TMANKI 355
A+ +
Sbjct: 158 MAASAL 163
>At1g23110.1 68414.m02889 hypothetical protein
Length = 261
Score = 26.2 bits (55), Expect = 9.7
Identities = 19/66 (28%), Positives = 31/66 (46%)
Frame = +2
Query: 158 KNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFSGNGDYSGVANPYISLSKTFSEMNPDYF 337
K +S +GV + LY A SR ++ K+ DY+ +A I LS+ NP +
Sbjct: 120 KVYANSLIGVGIASSLYHA-----SRGKLRKYLRWVDYTMIATTTICLSRALRNENPKFL 174
Query: 338 TMANKI 355
A+ +
Sbjct: 175 MAASAL 180
>At1g22420.1 68414.m02803 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 480
Score = 26.2 bits (55), Expect = 9.7
Identities = 18/51 (35%), Positives = 26/51 (50%)
Frame = -3
Query: 318 SENVLLKLMYGLATPL*SPLPENLSISARLPSPAPALYRSISIITPNGEDT 166
SENVL + +PL P P+N +P PA S+S+ PN ++T
Sbjct: 316 SENVLP--LVSATSPLDWPNPQNNLQPENVPPLVPATSPSMSLDFPNFQNT 364
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,344,427
Number of Sequences: 28952
Number of extensions: 193019
Number of successful extensions: 577
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 558
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 577
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 692941200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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