Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0006_B01
(373 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g25240.1 68415.m03020 serpin, putative / serine protease inhi... 61 2e-10
At1g47710.1 68414.m05302 serpin, putative / serine protease inhi... 59 8e-10
At1g62170.1 68414.m07013 serpin family protein / serine protease... 59 1e-09
At1g64030.1 68414.m07252 serpin family protein / serine protease... 56 8e-09
At3g45220.1 68416.m04880 serpin, putative / serine protease inhi... 56 1e-08
At2g35580.1 68415.m04357 serpin family protein / serine protease... 55 2e-08
At2g26390.1 68415.m03167 serpin, putative / serine protease inhi... 54 3e-08
At2g14540.1 68415.m01628 serpin family protein / serine protease... 54 4e-08
At1g51330.1 68414.m05772 serpin-related / serine protease inhibi... 48 2e-06
At1g63280.1 68414.m07154 serpin-related / serine protease inhibi... 42 1e-04
At1g21740.1 68414.m02721 expressed protein contains Pfam domains... 30 0.56
At3g48700.1 68416.m05318 expressed protein similar to PrMC3 [Pin... 28 2.3
At4g37920.1 68417.m05362 expressed protein 27 3.0
At2g46470.1 68415.m05783 OXA1 protein, putative similar to Oxa1 ... 27 3.0
At4g01290.1 68417.m00170 expressed protein 27 4.0
At5g52700.1 68418.m06542 heavy-metal-associated domain-containin... 27 5.3
At3g59180.1 68416.m06598 hypothetical protein contains a novel d... 27 5.3
At3g57670.1 68416.m06425 zinc finger (C2H2 type) protein (WIP2) ... 27 5.3
At1g04580.1 68414.m00451 aldehyde oxidase, putative similar to a... 27 5.3
At1g64340.1 68414.m07291 hypothetical protein 26 7.0
At5g44440.1 68418.m05446 FAD-binding domain-containing protein s... 26 9.2
At3g62600.1 68416.m07032 DNAJ heat shock family protein similar ... 26 9.2
At2g06990.1 68415.m00800 HUA enhancer 2 (HEN2) / DExH-box RNA he... 26 9.2
At1g21750.2 68414.m02723 protein disulfide isomerase, putative s... 26 9.2
At1g21750.1 68414.m02722 protein disulfide isomerase, putative s... 26 9.2
>At2g25240.1 68415.m03020 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 324
Score = 61.3 bits (142), Expect = 2e-10
Identities = 36/128 (28%), Positives = 61/128 (47%), Gaps = 4/128 (3%)
Frame = +1
Query: 1 ESSEYVLNFGSKIYIGQSVEPRQRFAAIAQEFYKTELKSVDFLNT-EVAANEINSWVSNT 177
E S+ L+ + ++I + + F + + YK VDF + +E+N+W
Sbjct: 21 EKSDLRLSIANGVWIDKFFSLKLSFKDLLENSYKATCSQVDFASKPSEVIDEVNTWAEVH 80
Query: 178 TQGKIPKLVDAD--DMIGMVVLII-NTLYFKGSWRHQFPPNMTKPGPFYVSPKSPKSVEF 348
T G I +++ D D I L++ N +YFKG+W +F NMTK F++ + V F
Sbjct: 81 TNGLIKQILSRDSIDTIRSSTLVLANAVYFKGAWSSKFDANMTKKNDFHLLDGTSVKVPF 140
Query: 349 MNVIDHFY 372
M + Y
Sbjct: 141 MTNYEDQY 148
>At1g47710.1 68414.m05302 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 391
Score = 59.3 bits (137), Expect = 8e-10
Identities = 32/113 (28%), Positives = 55/113 (48%), Gaps = 2/113 (1%)
Frame = +1
Query: 19 LNFGSKIYIGQSVEPRQRFAAIAQEFYKTELKSVDFLNTEVAA-NEINSWVSNTTQGKIP 195
L+ + +I +S+ + F + ++ YK DF + V E+NSW T G I
Sbjct: 92 LSVANGAWIDKSLSFKPSFKQLLEDSYKAASNQADFQSKAVEVIAEVNSWAEKETNGLIT 151
Query: 196 KLVDADDMIGMVVLII-NTLYFKGSWRHQFPPNMTKPGPFYVSPKSPKSVEFM 351
+++ M LI N LYFKG+W +F ++T+ G F++ + + FM
Sbjct: 152 EVLPEGSADSMTKLIFANALYFKGTWNEKFDESLTQEGEFHLLDGNKVTAPFM 204
>At1g62170.1 68414.m07013 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
GI:9937311 from [Cucurbita maxima]; contains Pfam
profile PF00079: Serpin (serine protease inhibitor)
Length = 433
Score = 58.8 bits (136), Expect = 1e-09
Identities = 32/114 (28%), Positives = 56/114 (49%), Gaps = 2/114 (1%)
Frame = +1
Query: 37 IYIGQSVEPRQRFAAIAQEFYKTELKSVDFLN-TEVAANEINSWVSNTTQGKIPKLVDAD 213
+++ QS+ + + F+ VDF + E E+N+W S+ T G I L+
Sbjct: 163 MWMDQSLSVNPLSKDLFKNFFSAAFAQVDFRSKAEEVRTEVNAWASSHTNGLIKDLLPRG 222
Query: 214 DMIGMVVLIINT-LYFKGSWRHQFPPNMTKPGPFYVSPKSPKSVEFMNVIDHFY 372
+ + + + LYFKG+W ++ +MTK PFY+ + SV FM+ + Y
Sbjct: 223 SVTSLTDRVYGSALYFKGTWEEKYSKSMTKCKPFYLLNGTSVSVPFMSSFEKQY 276
>At1g64030.1 68414.m07252 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
[Cucurbita maxima] GI:9937311, serpin [Triticum
aestivum] GI:871551; contains Pfam profile PF00079:
Serpin (serine protease inhibitor)
Length = 385
Score = 56.0 bits (129), Expect = 8e-09
Identities = 33/114 (28%), Positives = 55/114 (48%), Gaps = 2/114 (1%)
Frame = +1
Query: 37 IYIGQSVEPRQRFAAIAQEFYKTELKSVDFLN-TEVAANEINSWVSNTTQGKIPKLVDAD 213
++I +S+ +F + + F+K VDF + E E+NSWV + T I L+
Sbjct: 99 LWIDKSLPTDPKFKDLFENFFKAVYVPVDFRSEAEEVRKEVNSWVEHHTNNLIKDLLPDG 158
Query: 214 DMIGMVVLII-NTLYFKGSWRHQFPPNMTKPGPFYVSPKSPKSVEFMNVIDHFY 372
+ + I N L FKG+W+ F T+ FY+ + SV FM+ ++ Y
Sbjct: 159 SVTSLTNKIYANALSFKGAWKRPFEKYYTRDNDFYLVNGTSVSVPFMSSYENQY 212
>At3g45220.1 68416.m04880 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 393
Score = 55.6 bits (128), Expect = 1e-08
Identities = 33/122 (27%), Positives = 59/122 (48%), Gaps = 5/122 (4%)
Frame = +1
Query: 1 ESSEYVLNFGSKIYIGQSVEPRQRFAAIAQEFYKTELKSVDFLNTEVAA-NEINSWVSNT 177
E S+ L+ ++I +S+ + F + + Y VDF NE+N+W
Sbjct: 86 ERSDLHLSTAYGVWIDKSLSFKPSFKDLLENSYNATCNQVDFATKPAEVINEVNAWAEVH 145
Query: 178 TQGKIPKLVDADDMIGMV----VLIINTLYFKGSWRHQFPPNMTKPGPFYVSPKSPKSVE 345
T G I +++ +DD I + +++ N +YFKG+W +F +TK F++ + V
Sbjct: 146 TNGLIKEIL-SDDSIKTIRESMLILANAVYFKGAWSKKFDAKLTKSYDFHLLDGTMVKVP 204
Query: 346 FM 351
FM
Sbjct: 205 FM 206
>At2g35580.1 68415.m04357 serpin family protein / serine protease
inhibitor family protein similar to protein zx [Hordeum
vulgare subsp. vulgare] GI:19071, serpin [Triticum
aestivum] GI:1885350; contains Pfam profile PF00079:
Serpin (serine protease inhibitor)
Length = 374
Score = 54.8 bits (126), Expect = 2e-08
Identities = 31/108 (28%), Positives = 49/108 (45%), Gaps = 3/108 (2%)
Frame = +1
Query: 37 IYIGQSVEPRQRFAAIAQEFYKTELKSVDFLNTEVAAN-EINSWVSNTTQGKIPKLVDAD 213
++I +++ F + YK VDF N E+NSWV T G I L+ ++
Sbjct: 98 LWIEKTLNVEPSFKDLLLNSYKAAFNRVDFRTKADEVNREVNSWVEKQTNGLITNLLPSN 157
Query: 214 DMIGMVV--LIINTLYFKGSWRHQFPPNMTKPGPFYVSPKSPKSVEFM 351
+ + N L+F G W QF P++TK F++ + V FM
Sbjct: 158 PKSAPLTDHIFANALFFNGRWDSQFDPSLTKDSDFHLLDGTKVRVPFM 205
>At2g26390.1 68415.m03167 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 389
Score = 54.0 bits (124), Expect = 3e-08
Identities = 31/124 (25%), Positives = 56/124 (45%), Gaps = 7/124 (5%)
Frame = +1
Query: 1 ESSEYVLNFGSKIYIGQSVEPRQRFAAIAQEFYKTELKSVDFLNTEVAA-NEINSWVSNT 177
E S+ L+ ++I +S + F + + YK VDF V +E+N W
Sbjct: 83 ERSDLCLSTAHGVWIDKSSYLKPSFKELLENSYKASCSQVDFATKPVEVIDEVNIWADVH 142
Query: 178 TQGKIPKLVDAD------DMIGMVVLIINTLYFKGSWRHQFPPNMTKPGPFYVSPKSPKS 339
T G I +++ D ++ +++ N +YFK +W +F +TK F++ +
Sbjct: 143 TNGLIKQILSRDCTDTIKEIRNSTLILANAVYFKAAWSRKFDAKLTKDNDFHLLDGNTVK 202
Query: 340 VEFM 351
V FM
Sbjct: 203 VPFM 206
>At2g14540.1 68415.m01628 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 407
Score = 53.6 bits (123), Expect = 4e-08
Identities = 30/107 (28%), Positives = 51/107 (47%), Gaps = 2/107 (1%)
Frame = +1
Query: 37 IYIGQSVEPRQRFAAIAQEFYKTELKSVDFLN-TEVAANEINSWVSNTTQGKIPKLVDAD 213
+++ QS+ + + F+K VDF + E ++N+W S T I +++
Sbjct: 127 VWMEQSLSCNPDWEDLFLNFFKASFAKVDFRHKAEEVRLDVNTWASRHTNDLIKEILPRG 186
Query: 214 DMIGMVVLII-NTLYFKGSWRHQFPPNMTKPGPFYVSPKSPKSVEFM 351
+ + I N LYFKG+W F +MT+ PF++ SV FM
Sbjct: 187 SVTSLTNWIYGNALYFKGAWEKAFDKSMTRDKPFHLLNGKSVSVPFM 233
>At1g51330.1 68414.m05772 serpin-related / serine protease
inhibitor-related similar to serpin [Hordeum vulgare
subsp. vulgare] CAA64599.1 GI:1197577
Length = 193
Score = 48.0 bits (109), Expect = 2e-06
Identities = 25/75 (33%), Positives = 36/75 (48%), Gaps = 1/75 (1%)
Frame = +1
Query: 151 EINSWVSNTTQGKIPKLVDADDMIGMVVLII-NTLYFKGSWRHQFPPNMTKPGPFYVSPK 327
E+NSW T G I L+ + + I N LYFKG+W ++F +MT PF++
Sbjct: 38 EVNSWALRHTNGLIKNLLPPGSVTNQTIKIYGNALYFKGAWENKFGKSMTIHKPFHLVNG 97
Query: 328 SPKSVEFMNVIDHFY 372
V FM + Y
Sbjct: 98 KQVLVPFMKSYERKY 112
>At1g63280.1 68414.m07154 serpin-related / serine protease
inhibitor-related similar to protein zx [Hordeum vulgare
subsp. vulgare] GI:19071, serpin [Triticum aestivum]
GI:1885346
Length = 120
Score = 42.3 bits (95), Expect = 1e-04
Identities = 20/56 (35%), Positives = 30/56 (53%), Gaps = 1/56 (1%)
Frame = +1
Query: 151 EINSWVSNTTQGKIPKLVDADDMIGMVVLII-NTLYFKGSWRHQFPPNMTKPGPFY 315
E+N W S+ T G I L+ + V + N LYFKG+W ++F + TK F+
Sbjct: 15 ELNKWASDHTNGLIIDLLPRGSVKSETVQVYGNALYFKGAWENKFDKSSTKDNEFH 70
>At1g21740.1 68414.m02721 expressed protein contains Pfam domains,
PF04782: Protein of unknown function (DUF632) and
PF04783: Protein of unknown function (DUF630)
Length = 953
Score = 29.9 bits (64), Expect = 0.56
Identities = 15/42 (35%), Positives = 22/42 (52%)
Frame = +1
Query: 244 NTLYFKGSWRHQFPPNMTKPGPFYVSPKSPKSVEFMNVIDHF 369
NT YF + + PP+ +P P SP S +F+NV D +
Sbjct: 259 NTGYFGYPEQRREPPSPVRPTPAPPSPPRISSWDFLNVFDTY 300
>At3g48700.1 68416.m05318 expressed protein similar to PrMC3 [Pinus
radiata] GI:5487873
Length = 329
Score = 27.9 bits (59), Expect = 2.3
Identities = 9/21 (42%), Positives = 14/21 (66%)
Frame = +3
Query: 255 FQRKLASPVPTEYDKTWTILR 317
++R P+PT YD +WT L+
Sbjct: 115 YRRAPEHPIPTSYDDSWTALK 135
>At4g37920.1 68417.m05362 expressed protein
Length = 673
Score = 27.5 bits (58), Expect = 3.0
Identities = 18/55 (32%), Positives = 30/55 (54%), Gaps = 2/55 (3%)
Frame = +1
Query: 97 YKTELKSVDFLNTEVAANE--INSWVSNTTQGKIPKLVDADDMIGMVVLIINTLY 255
Y L+SV+ L+T A E +NS ++ KI L A ++ ++L+IN+ Y
Sbjct: 467 YDNTLESVETLDTAQAKFEDILNSPSVDSACEKIRSLAKAKELDSSLILLINSAY 521
>At2g46470.1 68415.m05783 OXA1 protein, putative similar to Oxa1
protein [Arabidopsis thaliana] GI:10176922
Length = 431
Score = 27.5 bits (58), Expect = 3.0
Identities = 11/35 (31%), Positives = 21/35 (60%)
Frame = +1
Query: 97 YKTELKSVDFLNTEVAANEINSWVSNTTQGKIPKL 201
+ ++ +DF+ TEV +EI V+ T+Q +P +
Sbjct: 89 WSDKVDGIDFVATEVVPDEIIEAVTTTSQAVVPAI 123
>At4g01290.1 68417.m00170 expressed protein
Length = 991
Score = 27.1 bits (57), Expect = 4.0
Identities = 10/19 (52%), Positives = 13/19 (68%)
Frame = +1
Query: 274 HQFPPNMTKPGPFYVSPKS 330
H+FPPNM PF+ +P S
Sbjct: 843 HRFPPNMIHRPPFHHTPTS 861
>At5g52700.1 68418.m06542 heavy-metal-associated domain-containing
protein contains Pfam profile PF00403:
Heavy-metal-associated domain
Length = 370
Score = 26.6 bits (56), Expect = 5.3
Identities = 10/26 (38%), Positives = 18/26 (69%)
Frame = -2
Query: 321 RYVEWSRFCHIRWELVTPASFEIQGV 244
RY++++ FC +R L+ S E++GV
Sbjct: 132 RYIQFTSFCIVRCLLLGVTSVEVKGV 157
>At3g59180.1 68416.m06598 hypothetical protein contains a novel
domain with similarity to F-box domain;
Length = 475
Score = 26.6 bits (56), Expect = 5.3
Identities = 11/33 (33%), Positives = 19/33 (57%)
Frame = -2
Query: 126 KVDRFQFSLVKLLSDRCKTLSWFNTLAYIYLTT 28
K+ F +++L+ CKT+ FN L Y+ + T
Sbjct: 269 KILYLSFDTLEVLNFCCKTIPLFNNLTYLTIET 301
>At3g57670.1 68416.m06425 zinc finger (C2H2 type) protein (WIP2)
identical to WIP2 protein [Arabidopsis thaliana]
gi|18027012|gb|AAL55722; contains Pfam domain, PF00096:
Zinc finger, C2H2 type
Length = 383
Score = 26.6 bits (56), Expect = 5.3
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +1
Query: 247 TLYFKGSWRHQFPPNMTKPGPFYVSPKSPKSVEF 348
T +FK + H +P + T P P + P +P S F
Sbjct: 10 TDWFKSNPFHHYPNSSTNPSPHPLPPVTPPSSFF 43
>At1g04580.1 68414.m00451 aldehyde oxidase, putative similar to
aldehyde oxidases from Arabidopsis thaliana: GI:3172023,
GI:3172025, GI:3172044; identical to cDNA putative
aldehyde oxidase (AO2) mRNA, partial cds GI:2792305
Length = 1337
Score = 26.6 bits (56), Expect = 5.3
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = +1
Query: 85 AQEFYKTELKSVDFLNTEVAANEI 156
A+ FYK E S ++LN V A+E+
Sbjct: 1143 ARTFYKPESSSAEYLNYGVGASEV 1166
>At1g64340.1 68414.m07291 hypothetical protein
Length = 265
Score = 26.2 bits (55), Expect = 7.0
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = +1
Query: 130 NTEVAANEINSWVSNTTQGKIPK 198
+TE+ +EINS V TT +IP+
Sbjct: 234 STEMVGSEINSLVRKTTSERIPQ 256
>At5g44440.1 68418.m05446 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 533
Score = 25.8 bits (54), Expect = 9.2
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = -1
Query: 94 TLERSLQNAVLVQHSGLYISYYRSLRRILK 5
TLERS +NAV +GLYI +L +++
Sbjct: 289 TLERSNKNAVHALFTGLYIGPVNNLLALME 318
>At3g62600.1 68416.m07032 DNAJ heat shock family protein similar to
DnaJ homolog subfamily B member 11 precursor
(SP:Q99KV1){Mus musculus}; contains Pfam PF00226: DnaJ
domain; contains PfaPF01556: DnaJ C terminal regionm
Length = 346
Score = 25.8 bits (54), Expect = 9.2
Identities = 13/38 (34%), Positives = 19/38 (50%)
Frame = +1
Query: 196 KLVDADDMIGMVVLIINTLYFKGSWRHQFPPNMTKPGP 309
K+V DD+I + + LY GS + N+ KP P
Sbjct: 131 KVVKGDDVIVELEATLEDLYMGGSMKVWREKNVIKPAP 168
>At2g06990.1 68415.m00800 HUA enhancer 2 (HEN2) / DExH-box RNA
helicase, putative nearly identical to HUA enhancer 2
[Arabidopsis thaliana] GI:16024936
Length = 995
Score = 25.8 bits (54), Expect = 9.2
Identities = 11/32 (34%), Positives = 17/32 (53%)
Frame = -1
Query: 139 LQCLKSRQISIQSCKTLERSLQNAVLVQHSGL 44
+QCL S+ + + + LQ + V HSGL
Sbjct: 378 MQCLNEEDRSLPAIELMLPLLQRGIAVHHSGL 409
>At1g21750.2 68414.m02723 protein disulfide isomerase, putative
similar to SP|P29828 Protein disulfide isomerase
precursor (PDI) (EC 5.3.4.1) {Medicago sativa}; isoform
contains non-consensus GA donor splice site at intron 9
Length = 487
Score = 25.8 bits (54), Expect = 9.2
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = +1
Query: 124 FLNTEVAANEINSWVSNTTQGKI 192
+L T V ++I SWV + GKI
Sbjct: 337 YLKTNVEVDQIESWVKDFKDGKI 359
>At1g21750.1 68414.m02722 protein disulfide isomerase, putative
similar to SP|P29828 Protein disulfide isomerase
precursor (PDI) (EC 5.3.4.1) {Medicago sativa}; isoform
contains non-consensus GA donor splice site at intron 9
Length = 501
Score = 25.8 bits (54), Expect = 9.2
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = +1
Query: 124 FLNTEVAANEINSWVSNTTQGKI 192
+L T V ++I SWV + GKI
Sbjct: 337 YLKTNVEVDQIESWVKDFKDGKI 359
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,684,020
Number of Sequences: 28952
Number of extensions: 181487
Number of successful extensions: 527
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 506
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 517
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 497853200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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