BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0006_A17
(323 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC364.05 |vps3||GTPase regulator Vps3 |Schizosaccharomyces pom... 27 0.70
SPBC1921.03c |mex67||mRNA export receptor Mex67|Schizosaccharomy... 25 3.7
SPAC23D3.03c |||GTPase activating protein |Schizosaccharomyces p... 24 6.5
SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce... 24 6.5
SPBC28F2.08c |||HRD ubiquitin ligase complex subunit |Schizosacc... 23 8.6
SPAC56F8.05c |mug64||conserved fungal protein|Schizosaccharomyce... 23 8.6
SPBC25B2.11 |pof2||F-box protein Pof2|Schizosaccharomyces pombe|... 23 8.6
SPAC19G12.14 |its3||1-phosphatidylinositol-4-phosphate 5-kinase ... 23 8.6
>SPCC364.05 |vps3||GTPase regulator Vps3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 910
Score = 27.1 bits (57), Expect = 0.70
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = +3
Query: 216 YKSKRFPKHLPKLRKLELSDNRISNGAEFSQWLVKN 323
Y+ +RF L K+R+ ++D S EF+ WL K+
Sbjct: 585 YEDRRFNDGLIKIREYLVNDIEPSTFWEFTTWLCKH 620
>SPBC1921.03c |mex67||mRNA export receptor Mex67|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 596
Score = 24.6 bits (51), Expect = 3.7
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = +3
Query: 246 PKLRKLELSDNRISNGAEFSQWLVK 320
PKL L L++NRI++ ++ W K
Sbjct: 240 PKLLNLSLANNRITSLSDLDPWSPK 264
>SPAC23D3.03c |||GTPase activating protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 472
Score = 23.8 bits (49), Expect = 6.5
Identities = 14/34 (41%), Positives = 17/34 (50%)
Frame = -2
Query: 322 FLTSH*ENSAPFDILLSDNSNFLSLGKCLGNLLD 221
F TS+ ++ LL N N S CL NLLD
Sbjct: 308 FDTSYIPGTSFIGALLLLNMNLTSAFNCLANLLD 341
>SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1242
Score = 23.8 bits (49), Expect = 6.5
Identities = 10/26 (38%), Positives = 14/26 (53%)
Frame = -3
Query: 84 DQPLFHFCKSVEFTLYVTVDSSIKYL 7
D PLFH CK YV + +++L
Sbjct: 18 DSPLFHPCKCTGSIRYVHQECLVEWL 43
>SPBC28F2.08c |||HRD ubiquitin ligase complex subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 713
Score = 23.4 bits (48), Expect = 8.6
Identities = 7/22 (31%), Positives = 13/22 (59%)
Frame = +2
Query: 41 SVNSTDLQKWKRGWSWNYGGGI 106
+++ + L W+ GW YG G+
Sbjct: 522 AIHPSALAYWRLGWMHEYGVGV 543
>SPAC56F8.05c |mug64||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 295
Score = 23.4 bits (48), Expect = 8.6
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = +1
Query: 70 EKRLVLELRGRNPSQVKELNLDNCRS 147
+KRL LE+ + K NL++C+S
Sbjct: 214 DKRLQLEMAKLDVESAKPENLEHCKS 239
>SPBC25B2.11 |pof2||F-box protein Pof2|Schizosaccharomyces pombe|chr
2|||Manual
Length = 463
Score = 23.4 bits (48), Expect = 8.6
Identities = 22/69 (31%), Positives = 34/69 (49%), Gaps = 6/69 (8%)
Frame = +1
Query: 61 TEMEKRLVLELRGRNPSQVKELNLDNCRS--TNIVG-LSDQYTNLEILSLNNAGLTNLKG 231
T + + L+ +L +N + V +N N S NI+ +SD NL+ L++ N GL G
Sbjct: 105 TRISEPLIGKLLYQNLNLVT-INFSNIFSLPANILEYISDNCPNLKALNIGNCGLVEDTG 163
Query: 232 FPSI---CP 249
I CP
Sbjct: 164 MVQIIKRCP 172
>SPAC19G12.14 |its3||1-phosphatidylinositol-4-phosphate 5-kinase
Its3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 742
Score = 23.4 bits (48), Expect = 8.6
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = -1
Query: 218 VRPALFKLRISRFVY*SESPTMLVLR 141
V PA + R +FV S PT+LVL+
Sbjct: 645 VPPAEYASRFYKFVESSIKPTLLVLK 670
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,323,450
Number of Sequences: 5004
Number of extensions: 25257
Number of successful extensions: 67
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 66
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 67
length of database: 2,362,478
effective HSP length: 64
effective length of database: 2,042,222
effective search space used: 87815546
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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