Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0005_P22
(343 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g54030.1 68418.m06720 DC1 domain-containing protein contains ... 31 0.20
At5g57010.1 68418.m07115 calmodulin-binding family protein conta... 29 1.1
At5g56050.1 68418.m06993 hypothetical protein 28 1.9
At3g01460.1 68416.m00070 PHD finger family protein / methyl-CpG ... 28 1.9
At5g60920.1 68418.m07642 phytochelatin synthetase, putative / CO... 27 3.3
At2g01270.1 68415.m00040 thioredoxin family protein low similari... 27 4.3
At3g57180.1 68416.m06366 expressed protein 26 7.6
At2g43220.1 68415.m05372 DC1 domain-containing protein contains ... 26 7.6
At2g35340.1 68415.m04333 RNA helicase, putative similar to ATP-d... 26 7.6
At3g24000.1 68416.m03014 pentatricopeptide (PPR) repeat-containi... 25 10.0
At3g17750.1 68416.m02265 protein kinase family protein contains ... 25 10.0
At2g35550.2 68415.m04355 expressed protein 25 10.0
At2g35550.1 68415.m04354 expressed protein 25 10.0
At1g27910.1 68414.m03420 U-box domain-containing protein contain... 25 10.0
At1g15020.2 68414.m01795 thioredoxin family protein low similari... 25 10.0
At1g15020.1 68414.m01794 thioredoxin family protein low similari... 25 10.0
>At5g54030.1 68418.m06720 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 419
Score = 31.1 bits (67), Expect = 0.20
Identities = 18/68 (26%), Positives = 29/68 (42%)
Frame = +2
Query: 131 KIYCPVCGSYGKTYENPCEFYCQKDKTHSNMTIVKNTACEVGIPCYCTLEYAPVCGSNGQ 310
K+Y + S G+ YEN C F CQ + + T C + + C P+ S +
Sbjct: 83 KVYTHIFTSSGRIYENTCHF-CQ---SKLEFLFARCTICNLNVDIECLFALPPLTISEPK 138
Query: 311 TYANKCSL 334
+ + SL
Sbjct: 139 HHKHSLSL 146
>At5g57010.1 68418.m07115 calmodulin-binding family protein contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 495
Score = 28.7 bits (61), Expect = 1.1
Identities = 16/40 (40%), Positives = 19/40 (47%)
Frame = +2
Query: 131 KIYCPVCGSYGKTYENPCEFYCQKDKTHSNMTIVKNTACE 250
K P G Y T EN EF C + H N+T VK A +
Sbjct: 354 KAVWPYSGHYLPTEENFREFICFLRENHVNLTNVKMNAID 393
>At5g56050.1 68418.m06993 hypothetical protein
Length = 283
Score = 27.9 bits (59), Expect = 1.9
Identities = 12/30 (40%), Positives = 15/30 (50%), Gaps = 1/30 (3%)
Frame = +3
Query: 234 KTPHARWASPATVPWNMPP-SVALTDKLTP 320
+TP ++W SP PW P S T TP
Sbjct: 21 ETPSSKWYSPIYTPWRTTPRSTQSTPTTTP 50
>At3g01460.1 68416.m00070 PHD finger family protein / methyl-CpG
binding domain-containing protein contains Pfam profiles
PF00628: PHD-finger (2 copies), PF01429: Methyl-CpG
binding domain
Length = 2176
Score = 27.9 bits (59), Expect = 1.9
Identities = 14/54 (25%), Positives = 27/54 (50%)
Frame = -3
Query: 224 SYCCVSCLFGSKIHTGSRTSYHMTRRRDNRFFRKYITAVAELLPSILAQRRSEE 63
++ C SC+ ++ + SY + RRR R ++ +T + L + LA E+
Sbjct: 1328 NWYCPSCVIAKRMAQEALESYKLVRRRKGRKYQGELTRASMELTAHLADVMEEK 1381
>At5g60920.1 68418.m07642 phytochelatin synthetase, putative / COBRA
cell expansion protein COB, putative similar to
phytochelatin synthetase GI:29570314; similar to
GB:AAK56072; identified in Roudier, et al, Plant Phys.
(2002) 130:538-548 (PMID:12376623); identical to cDNA
putative phytochelatin synthetase GI:3559804; contains
Pfam profile PF04833: Phytochelatin synthetase-like
conserved region
Length = 456
Score = 27.1 bits (57), Expect = 3.3
Identities = 15/36 (41%), Positives = 18/36 (50%), Gaps = 2/36 (5%)
Frame = +3
Query: 237 TPH-ARWASPATVPWN-MPPSVALTDKLTPTNVHWN 338
TPH A SP T +PP V T + P VHW+
Sbjct: 270 TPHLASVVSPPTKKGTVLPPLVQCTRHMCPIRVHWH 305
>At2g01270.1 68415.m00040 thioredoxin family protein low similarity
to quiescin [Homo sapiens] GI:13257405; contains Pfam
profiles PF00085: Thioredoxin, PF04777: Erv1 / Alr
family
Length = 495
Score = 26.6 bits (56), Expect = 4.3
Identities = 9/37 (24%), Positives = 15/37 (40%)
Frame = +2
Query: 137 YCPVCGSYGKTYENPCEFYCQKDKTHSNMTIVKNTAC 247
+CP C +Y YE + D H + ++ C
Sbjct: 65 WCPACRNYKPHYEKVARLFNGPDAIHPGIVLMTRVDC 101
>At3g57180.1 68416.m06366 expressed protein
Length = 644
Score = 25.8 bits (54), Expect = 7.6
Identities = 12/31 (38%), Positives = 16/31 (51%)
Frame = +2
Query: 128 GKIYCPVCGSYGKTYENPCEFYCQKDKTHSN 220
GKI CP CG + + + Y QK K +N
Sbjct: 94 GKIICPGCGIFMQDNDPDLPGYYQKRKVIAN 124
>At2g43220.1 68415.m05372 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 538
Score = 25.8 bits (54), Expect = 7.6
Identities = 13/40 (32%), Positives = 15/40 (37%)
Frame = -3
Query: 278 PRYSSRGCPPRMRCFSRLSYCCVSCLFGSKIHTGSRTSYH 159
P YS C R S Y C C F + G+R H
Sbjct: 77 PSYSDEKCSLCQRKLSNFVYHCKLCNFSIDMECGNRPPPH 116
>At2g35340.1 68415.m04333 RNA helicase, putative similar to
ATP-dependent RNA helicase #3 [Homo sapiens] GI:3107913;
contains Pfam profiles PF04408: Helicase associated
domain (HA2), PF00271: Helicase conserved C-terminal
domain
Length = 1110
Score = 25.8 bits (54), Expect = 7.6
Identities = 14/43 (32%), Positives = 24/43 (55%), Gaps = 3/43 (6%)
Frame = +1
Query: 169 VREP---V*ILLPKRQDTQQYDNREKHRMRGGHPLLLYLGICP 288
V+EP V + LP +++ + + KH++RG + L ICP
Sbjct: 674 VKEPLGDVLVFLPGQEEIEAVEENLKHKIRGLGTKIRELIICP 716
>At3g24000.1 68416.m03014 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 633
Score = 25.4 bits (53), Expect = 10.0
Identities = 13/30 (43%), Positives = 15/30 (50%)
Frame = -3
Query: 263 RGCPPRMRCFSRLSYCCVSCLFGSKIHTGS 174
RGC C +L CV C F S +H GS
Sbjct: 176 RGC-----CGHQLHGFCVKCGFDSNVHVGS 200
>At3g17750.1 68416.m02265 protein kinase family protein contains
Pfam profile: PF00069 Eukaryotic protein kinase domain
Length = 1138
Score = 25.4 bits (53), Expect = 10.0
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = -3
Query: 164 YHMTRRRDNRFFRKYITAVAELLPSIL 84
YH+ R D +FR+++ V ELL + L
Sbjct: 887 YHLLRLYDYFYFREHLLIVCELLKANL 913
>At2g35550.2 68415.m04355 expressed protein
Length = 226
Score = 25.4 bits (53), Expect = 10.0
Identities = 13/50 (26%), Positives = 21/50 (42%)
Frame = +2
Query: 14 SRFVPLIFKHKMNKLGXXXXXXXXXYSVTALPPPLCICGKIYCPVCGSYG 163
S+ P I + K K + + +PPP+C C + VC +G
Sbjct: 103 SKKTPSIPETKREKKNLDINIDISSFDTSGVPPPVCSCTGV-SRVCYKWG 151
>At2g35550.1 68415.m04354 expressed protein
Length = 271
Score = 25.4 bits (53), Expect = 10.0
Identities = 13/50 (26%), Positives = 21/50 (42%)
Frame = +2
Query: 14 SRFVPLIFKHKMNKLGXXXXXXXXXYSVTALPPPLCICGKIYCPVCGSYG 163
S+ P I + K K + + +PPP+C C + VC +G
Sbjct: 148 SKKTPSIPETKREKKNLDINIDISSFDTSGVPPPVCSCTGV-SRVCYKWG 196
>At1g27910.1 68414.m03420 U-box domain-containing protein contains
Pfam profile PF04564: U-box domain
Length = 768
Score = 25.4 bits (53), Expect = 10.0
Identities = 14/45 (31%), Positives = 21/45 (46%)
Frame = +2
Query: 194 CQKDKTHSNMTIVKNTACEVGIPCYCTLEYAPVCGSNGQTYANKC 328
C ++ S M++ E+ P L Y PV ++GQTY C
Sbjct: 267 CNNNRRSSQMSVPPE---ELRCPISLQLMYDPVIIASGQTYERIC 308
>At1g15020.2 68414.m01795 thioredoxin family protein low similarity
to FAD-dependent sulfhydryl oxidase-2 [Rattus
norvegicus] GI:12483919; contains Pfam profiles PF00085:
Thioredoxin, PF04777: Erv1 / Alr family
Length = 528
Score = 25.4 bits (53), Expect = 10.0
Identities = 8/39 (20%), Positives = 16/39 (41%)
Frame = +2
Query: 137 YCPVCGSYGKTYENPCEFYCQKDKTHSNMTIVKNTACEV 253
+CP C +Y YE + D + + ++ C +
Sbjct: 71 WCPACRNYKPHYEKVARLFNGADAVYPGVVLMTRVDCAI 109
>At1g15020.1 68414.m01794 thioredoxin family protein low similarity
to FAD-dependent sulfhydryl oxidase-2 [Rattus
norvegicus] GI:12483919; contains Pfam profiles PF00085:
Thioredoxin, PF04777: Erv1 / Alr family
Length = 502
Score = 25.4 bits (53), Expect = 10.0
Identities = 8/39 (20%), Positives = 16/39 (41%)
Frame = +2
Query: 137 YCPVCGSYGKTYENPCEFYCQKDKTHSNMTIVKNTACEV 253
+CP C +Y YE + D + + ++ C +
Sbjct: 71 WCPACRNYKPHYEKVARLFNGADAVYPGVVLMTRVDCAI 109
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,297,310
Number of Sequences: 28952
Number of extensions: 177576
Number of successful extensions: 526
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 512
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 526
length of database: 12,070,560
effective HSP length: 72
effective length of database: 9,986,016
effective search space used: 409426656
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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