BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0005_O22 (578 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g74260.1 68414.m08600 AIR synthase-related family protein con... 56 1e-08 At5g03555.1 68418.m00313 permease, cytosine/purines, uracil, thi... 33 0.18 At5g62760.2 68418.m07879 nuclear protein ZAP-related similar to ... 31 0.56 At5g62760.1 68418.m07878 nuclear protein ZAP-related similar to ... 31 0.56 At1g42440.1 68414.m04894 expressed protein contains Pfam domain,... 31 0.73 At1g77910.1 68414.m09079 hypothetical protein contains similarit... 29 1.7 At1g54200.1 68414.m06178 expressed protein 29 2.2 At5g05030.1 68418.m00534 expressed protein contains similarity t... 28 5.2 At3g14205.1 68416.m01795 phosphoinositide phosphatase family pro... 27 9.0 >At1g74260.1 68414.m08600 AIR synthase-related family protein contains Pfam profiles: PF00586 AIR synthase related protein, N-terminal domain, PF02769 AIR synthase related protein, C-terminal domain Length = 1387 Score = 56.4 bits (130), Expect = 1e-08 Identities = 26/50 (52%), Positives = 30/50 (60%) Frame = +3 Query: 3 DGRHIAMMPHPERCTMRWQCPTDAPKPLVSSDPTCQYSPWLRLFQNAYLW 152 DGRH+AMMPHPERC + WQ P P SPWL++FQNA W Sbjct: 1337 DGRHLAMMPHPERCFLMWQFPW---YPTSWDVEKAGPSPWLKMFQNARDW 1383 >At5g03555.1 68418.m00313 permease, cytosine/purines, uracil, thiamine, allantoin family protein contains Pfam PF02133: permease, cytosine/purines, uracil, thiamine, allantoin family Length = 599 Score = 32.7 bits (71), Expect = 0.18 Identities = 21/74 (28%), Positives = 40/74 (54%) Frame = +3 Query: 327 LMLHINKIGYKYS*RSLKPKLKSDYQ*GIYEHFNFILEESAVY*SMTSCIKRFVLRERDR 506 L LH+N +K++ SL L+S + +Y+H +F +S+ S TSC+ F ++ + Sbjct: 8 LSLHLNLHPHKHNRHSLS-SLRSRTKAKLYQHVSFT--DSSHKSSYTSCVSTFDIQRKSS 64 Query: 507 TSLDSHQHL*TPII 548 + +H +PI+ Sbjct: 65 KHYELGKHSFSPIL 78 >At5g62760.2 68418.m07879 nuclear protein ZAP-related similar to nuclear protein ZAP, Mus musculus, EMBL:AB033168 this cDNA provides a truncated ORF likely due to a skipped exon. An alternative ORF is provided. Length = 383 Score = 31.1 bits (67), Expect = 0.56 Identities = 18/43 (41%), Positives = 20/43 (46%), Gaps = 3/43 (6%) Frame = +1 Query: 13 ISP*CHIPSVALCVGNAP--RMHRNPSFPPTPLANIPL-GFDS 132 I P C +P C P NP+FPP P N P GFDS Sbjct: 29 ICPICTVPHFPFCPPYPPPSSFAYNPNFPPPPHLNSPRPGFDS 71 >At5g62760.1 68418.m07878 nuclear protein ZAP-related similar to nuclear protein ZAP, Mus musculus, EMBL:AB033168 this cDNA provides a truncated ORF likely due to a skipped exon. An alternative ORF is provided. Length = 661 Score = 31.1 bits (67), Expect = 0.56 Identities = 18/43 (41%), Positives = 20/43 (46%), Gaps = 3/43 (6%) Frame = +1 Query: 13 ISP*CHIPSVALCVGNAP--RMHRNPSFPPTPLANIPL-GFDS 132 I P C +P C P NP+FPP P N P GFDS Sbjct: 29 ICPICTVPHFPFCPPYPPPSSFAYNPNFPPPPHLNSPRPGFDS 71 >At1g42440.1 68414.m04894 expressed protein contains Pfam domain, PF04950: Protein of unknown function (DUF663) Length = 793 Score = 30.7 bits (66), Expect = 0.73 Identities = 15/41 (36%), Positives = 23/41 (56%) Frame = +1 Query: 85 SFPPTPLANIPLGFDSSKTHISGLRTIKIIPYNKLILFRFI 207 SFPP PL + + S I+ L ++K + NK+IL + I Sbjct: 665 SFPPLPLVVLKISEGSDPPAIAALGSLKSVEPNKIILKKII 705 >At1g77910.1 68414.m09079 hypothetical protein contains similarity to NADH dehydrogenase subunit 1 Length = 89 Score = 29.5 bits (63), Expect = 1.7 Identities = 15/33 (45%), Positives = 24/33 (72%), Gaps = 1/33 (3%) Frame = +3 Query: 243 QYIYQFVSPLRMVFVIYLLCLQIFIEYFL-MLH 338 +YI+ ++ PL + F+I+LLC I+ EY L +LH Sbjct: 49 KYIFIYMPPL-IDFLIFLLCFVIYGEYLLCILH 80 >At1g54200.1 68414.m06178 expressed protein Length = 366 Score = 29.1 bits (62), Expect = 2.2 Identities = 15/34 (44%), Positives = 18/34 (52%) Frame = +1 Query: 67 RMHRNPSFPPTPLANIPLGFDSSKTHISGLRTIK 168 R HR+PSF T L I D S T+ S +R K Sbjct: 21 RDHRHPSFSSTLLDQIYRSIDDSSTNSSSMRKTK 54 >At5g05030.1 68418.m00534 expressed protein contains similarity to carboxyl-terminal proteinase contains Pfam profile PF03080: Arabidopsis proteins of unknown function Length = 363 Score = 27.9 bits (59), Expect = 5.2 Identities = 13/26 (50%), Positives = 16/26 (61%) Frame = +1 Query: 115 PLGFDSSKTHISGLRTIKIIPYNKLI 192 PL DS THI+G+RT PY +I Sbjct: 111 PLTIDSHGTHIAGVRTTDQGPYRGVI 136 >At3g14205.1 68416.m01795 phosphoinositide phosphatase family protein contains similarity to phosphoinositide phosphatase SAC1 [Rattus norvegicus] gi|11095248|gb|AAG29810; contains Pfam domain, PF02383: SacI homology domain; identical to SAC domain protein 2 (SAC2) GI:31415720 Length = 808 Score = 27.1 bits (57), Expect = 9.0 Identities = 19/54 (35%), Positives = 29/54 (53%), Gaps = 1/54 (1%) Frame = -3 Query: 267 EKRIDKCIDLQLIFYTQFRFDETK*YELVIGDYFNGSKPRDMRFGRVE-AKGNI 109 E+ IDK +D+ F +FR ET+ +IG N + R ++ R E A+ NI Sbjct: 5 ERSIDKEVDMGSSFLQKFRLYETRSSFYMIGRDKNRTSWRVLKLDRTEPAEVNI 58 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,449,641 Number of Sequences: 28952 Number of extensions: 257390 Number of successful extensions: 642 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 626 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 641 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1131744440 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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