BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0005_N19 (505 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|c... 29 0.40 SPAC20H4.06c |||RNA-binding protein|Schizosaccharomyces pombe|ch... 29 0.52 SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|ch... 28 0.69 SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr... 27 2.1 SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 26 2.8 SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccha... 26 3.7 SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces... 26 3.7 SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 25 6.5 SPBC28F2.08c |||HRD ubiquitin ligase complex subunit |Schizosacc... 25 8.5 SPBC1604.13c |mrpl32||mitochondrial ribosomal protein subunit L3... 25 8.5 SPBC21B10.03c |||ataxin-2 homolog|Schizosaccharomyces pombe|chr ... 25 8.5 >SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|chr 1|||Manual Length = 589 Score = 29.1 bits (62), Expect = 0.40 Identities = 15/38 (39%), Positives = 22/38 (57%) Frame = -3 Query: 176 CILGFTLSACFISRTRAVKPSLCSDSLSSTGVVPNVFL 63 CI GF ++ + +RTR + PS +LS T V+ FL Sbjct: 325 CIAGFVVNEFYTTRTRIITPS-AFKTLSLTSVMVTSFL 361 >SPAC20H4.06c |||RNA-binding protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 534 Score = 28.7 bits (61), Expect = 0.52 Identities = 14/30 (46%), Positives = 19/30 (63%), Gaps = 3/30 (10%) Frame = -1 Query: 346 RGSAVRKSVQCS---PPLWGYVQVYNSQMP 266 +G ++ KS CS PPL G+V V+N Q P Sbjct: 256 KGKSIIKSQNCSDGLPPLPGFVVVFNLQSP 285 >SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 589 Score = 28.3 bits (60), Expect = 0.69 Identities = 14/38 (36%), Positives = 22/38 (57%) Frame = -3 Query: 176 CILGFTLSACFISRTRAVKPSLCSDSLSSTGVVPNVFL 63 CI GF ++ + +RTR + PS +LS + V+ FL Sbjct: 325 CIAGFVVNELYTTRTRIIAPS-AFQTLSLSSVMVTSFL 361 >SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 780 Score = 26.6 bits (56), Expect = 2.1 Identities = 12/31 (38%), Positives = 17/31 (54%), Gaps = 1/31 (3%) Frame = +2 Query: 404 PTNLSL-VSAPLDVRGSSIQTVEIPEFHRYT 493 PT L SA DVR +S + +FH+Y+ Sbjct: 51 PTELKARTSATFDVRSASTSPINASDFHKYS 81 >SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces pombe|chr 2|||Manual Length = 1485 Score = 26.2 bits (55), Expect = 2.8 Identities = 21/68 (30%), Positives = 30/68 (44%), Gaps = 5/68 (7%) Frame = +1 Query: 94 DKESEHREG---FTALVRE--MKQALNVKPNMQLVISVLPNVNSSIYFDVPSIINLVDIV 258 D ES H EG F + E MK+ALN ++ +S ++ I FD I D V Sbjct: 985 DYESHHGEGNVHFNVTLTEAGMKEALNESLEVKFKLSRTQATSNMIAFDASGRIKKYDSV 1044 Query: 259 NIQAFDYY 282 ++Y Sbjct: 1045 EDILTEFY 1052 >SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccharomyces pombe|chr 1|||Manual Length = 569 Score = 25.8 bits (54), Expect = 3.7 Identities = 14/39 (35%), Positives = 22/39 (56%), Gaps = 3/39 (7%) Frame = +2 Query: 389 KMVRLPTNLSLVSAPLDVR---GSSIQTVEIPEFHRYTP 496 +++ +P NL+ PL GSS +T ++PEF TP Sbjct: 144 ELIMVPPNLA--GKPLSANSSFGSSAKTFQLPEFDSVTP 180 >SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1009 Score = 25.8 bits (54), Expect = 3.7 Identities = 10/22 (45%), Positives = 12/22 (54%) Frame = -2 Query: 258 DNVDQVYDAWDVKVNGAIYVWQ 193 D VD++YDAW N WQ Sbjct: 54 DYVDEMYDAWKKDPNSVHASWQ 75 >SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizosaccharomyces pombe|chr 3|||Manual Length = 139 Score = 25.0 bits (52), Expect = 6.5 Identities = 11/32 (34%), Positives = 16/32 (50%) Frame = +1 Query: 217 YFDVPSIINLVDIVNIQAFDYYTPERNPKEAD 312 Y D P I+ V +N+ D T + NP + D Sbjct: 68 YPDAPPIVTFVSRINLPGVDGETGKVNPHKID 99 >SPBC28F2.08c |||HRD ubiquitin ligase complex subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 713 Score = 24.6 bits (51), Expect = 8.5 Identities = 12/40 (30%), Positives = 20/40 (50%) Frame = +1 Query: 289 ERNPKEADYTAPIYAPQNRDPLQNADAAINYWIQNGAPTH 408 +R+P + Y + P N +NA A+NY+ + A H Sbjct: 451 DRDPSTS-YLKTVIFPTNEQTHRNARIAMNYYSRAAARNH 489 >SPBC1604.13c |mrpl32||mitochondrial ribosomal protein subunit L32|Schizosaccharomyces pombe|chr 2|||Manual Length = 103 Score = 24.6 bits (51), Expect = 8.5 Identities = 13/40 (32%), Positives = 21/40 (52%) Frame = +1 Query: 127 ALVREMKQALNVKPNMQLVISVLPNVNSSIYFDVPSIINL 246 AL+R A++ K S LP+++ I+ PSI N+ Sbjct: 2 ALLRGNSLAISQKMLSVFQASALPHISLRIFISPPSIANI 41 >SPBC21B10.03c |||ataxin-2 homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 791 Score = 24.6 bits (51), Expect = 8.5 Identities = 15/43 (34%), Positives = 18/43 (41%) Frame = +1 Query: 277 YYTPERNPKEADYTAPIYAPQNRDPLQNADAAINYWIQNGAPT 405 Y TP Y P Y P R+P Q+ A + NG PT Sbjct: 590 YPTPTMPYIPVGYPVPGYTPYMRNPSQHTSVAPS---PNGTPT 629 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.314 0.133 0.397 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,162,027 Number of Sequences: 5004 Number of extensions: 44350 Number of successful extensions: 113 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 110 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 113 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 200198394 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 42 (21.9 bits)
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