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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0005_M23
         (492 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_2391| Best HMM Match : No HMM Matches (HMM E-Value=.)               39   0.003
SB_16018| Best HMM Match : Antimicrobial18 (HMM E-Value=0.89)          33   0.097
SB_52639| Best HMM Match : 7tm_1 (HMM E-Value=0)                       29   1.6  
SB_43870| Best HMM Match : Pkinase_Tyr (HMM E-Value=0)                 29   2.8  
SB_59385| Best HMM Match : No HMM Matches (HMM E-Value=.)              27   6.4  
SB_52196| Best HMM Match : No HMM Matches (HMM E-Value=.)              27   6.4  
SB_7912| Best HMM Match : No HMM Matches (HMM E-Value=.)               27   6.4  
SB_7844| Best HMM Match : RasGEF (HMM E-Value=0.00058)                 27   8.4  

>SB_2391| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 282

 Score = 38.7 bits (86), Expect = 0.003
 Identities = 17/63 (26%), Positives = 32/63 (50%)
 Frame = +1

Query: 280 EIKSFAEVEQTVKTAKCECGDKEHAFLAIKEIDDNYYVVYSAPFTPNGLTLIVLNARTIP 459
           ++  F ++   +K  K  C  K HA     E+ ++ +  Y   FTPN   +++++  TIP
Sbjct: 192 DVHRFEKISNIIKQFKLSCS-KLHAHFQSMEVKNSGFAAYIDVFTPNTYVMVIMSDTTIP 250

Query: 460 TQA 468
           + A
Sbjct: 251 SAA 253


>SB_16018| Best HMM Match : Antimicrobial18 (HMM E-Value=0.89)
          Length = 1494

 Score = 33.5 bits (73), Expect = 0.097
 Identities = 27/96 (28%), Positives = 45/96 (46%), Gaps = 1/96 (1%)
 Frame = -2

Query: 344 LSPHSHFAVFTVCSTSAKDFISTAANRASEFLSDSCL-IEINGTLVLPSQVQWTSLTLVT 168
           L+  S  +VF   S+S  DF+ T+++  S FL+ S L  +   +  LPS    +S +L  
Sbjct: 568 LTSSSSLSVFLTSSSSLSDFL-TSSSSLSVFLTSSSLPSDFLTSSSLPSDFLTSSSSLSV 626

Query: 167 VNQSQPVPSSVMLGDMPMETALPRNLSISA*ATTKS 60
              S  +PS  +     +   L  + S+S   T+ S
Sbjct: 627 FPTSSSLPSDFLTSSSSLSDFLTSSSSLSVFPTSSS 662



 Score = 29.9 bits (64), Expect = 1.2
 Identities = 27/111 (24%), Positives = 50/111 (45%), Gaps = 4/111 (3%)
 Frame = -2

Query: 380  SSISLIAKNACSLSPH----SHFAVFTVCSTSAKDFISTAANRASEFLSDSCLIEINGTL 213
            SS+S+   ++ SLS      S  + F   S+S  DF++++++ +    S S   +   + 
Sbjct: 817  SSLSVFLTSSSSLSDFLTSSSSLSDFLTSSSSLSDFLTSSSSLSVFPTSSSLPSDFLTSS 876

Query: 212  VLPSQVQWTSLTLVTVNQSQPVPSSVMLGDMPMETALPRNLSISA*ATTKS 60
             LPS    +S +L     S  +PS  +     +   L  + S+S   T+ S
Sbjct: 877  SLPSDFLTSSSSLSVFPTSSSLPSDFLTSSSSLSDFLTSSSSLSVFPTSSS 927


>SB_52639| Best HMM Match : 7tm_1 (HMM E-Value=0)
          Length = 359

 Score = 29.5 bits (63), Expect = 1.6
 Identities = 16/45 (35%), Positives = 22/45 (48%)
 Frame = -1

Query: 351 VLFVTAFTFRCFHRLFYFCEGFYFHGCESCIRILI*FLFNRN*RD 217
           ++F +  TF CF + FYF   F  H   S I   +   FN N R+
Sbjct: 267 IMFFSPQTFPCFSKPFYFVSLFLGHS-NSAINPFLYSFFNANFRE 310


>SB_43870| Best HMM Match : Pkinase_Tyr (HMM E-Value=0)
          Length = 869

 Score = 28.7 bits (61), Expect = 2.8
 Identities = 26/82 (31%), Positives = 37/82 (45%), Gaps = 6/82 (7%)
 Frame = +1

Query: 115 IGISPNITELGTGCDWFTVTKVSEVHCTCDGRTNVPLISIKQE--SDKNSDAR----FAA 276
           I I+P I ++ T    FTV + +E+ CTC        I  K    SD+NS  R    + A
Sbjct: 123 IRIAPQIIDITTNFT-FTVGQTAELMCTCYFNALDDTIEFKWHAISDRNSVCRNPFSYNA 181

Query: 277 VEIKSFAEVEQTVKTAKCECGD 342
           V +KS  + +  V       GD
Sbjct: 182 VVVKSDDDNDDGVSDVDDASGD 203


>SB_59385| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1038

 Score = 27.5 bits (58), Expect = 6.4
 Identities = 11/31 (35%), Positives = 15/31 (48%)
 Frame = -1

Query: 354  CVLFVTAFTFRCFHRLFYFCEGFYFHGCESC 262
            C  F T  T +CFHR       + F G ++C
Sbjct: 917  CQYFTTETTCKCFHRSELRQVSYIFRGAKNC 947


>SB_52196| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 428

 Score = 27.5 bits (58), Expect = 6.4
 Identities = 10/25 (40%), Positives = 15/25 (60%)
 Frame = +1

Query: 277 VEIKSFAEVEQTVKTAKCECGDKEH 351
           VE+ ++A+ EQ     KC C  K+H
Sbjct: 266 VEVHTYADDEQDCCQGKCNCSHKKH 290


>SB_7912| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1020

 Score = 27.5 bits (58), Expect = 6.4
 Identities = 18/67 (26%), Positives = 30/67 (44%)
 Frame = -2

Query: 329 HFAVFTVCSTSAKDFISTAANRASEFLSDSCLIEINGTLVLPSQVQWTSLTLVTVNQSQP 150
           H  V  +C  S     S  AN+     + + L+E     +  ++V W  LT   ++ SQ 
Sbjct: 171 HHLVDALCQLSTTSMESAQANKEPSLFAVAKLLETGLNNLHRARVLWKPLTAHLLDVSQV 230

Query: 149 VPSSVML 129
           V S ++L
Sbjct: 231 VYSIMLL 237


>SB_7844| Best HMM Match : RasGEF (HMM E-Value=0.00058)
          Length = 1299

 Score = 27.1 bits (57), Expect = 8.4
 Identities = 24/127 (18%), Positives = 51/127 (40%), Gaps = 3/127 (2%)
 Frame = +1

Query: 121 ISPNITELGTGCDWFTVTKVSEVHCTCDGRTNVPLISIKQESDKNSDARFAAVEIKSFAE 300
           ++ + +++ T  D       S+V  T D         +   SD       +A++I +  +
Sbjct: 578 VTLSASDVPTTSDDQVTLSASDVPTTSDDPVTPSASDVTPTSDDQVTP--SAIDIPTAKD 635

Query: 301 VEQTVKTAKCECGDKEHAFLAIKEI---DDNYYVVYSAPFTPNGLTLIVLNARTIPTQAD 471
            + T+          +   L+  ++    D+   + ++  TP     + L+ R IPT++D
Sbjct: 636 DKVTLSAMNVPTAKDDKVTLSASDVPTTSDDQVTLSASDVTPTSDNQVTLSPRDIPTKSD 695

Query: 472 LDKTVSG 492
              T SG
Sbjct: 696 DQVTPSG 702


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,412,826
Number of Sequences: 59808
Number of extensions: 276145
Number of successful extensions: 665
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 590
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 661
length of database: 16,821,457
effective HSP length: 77
effective length of database: 12,216,241
effective search space used: 1050596726
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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