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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0005_M19
         (553 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po...   115   5e-27
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ...    76   3e-15
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po...    54   2e-08
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha...    41   1e-04
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc...    36   0.003
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c...    35   0.009
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac...    32   0.049
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ...    31   0.15 
SPAC17G8.03c |dpb3||DNA polymerase epsilon subunit Dpb3|Schizosa...    27   1.8  
SPCC1322.06 |kap113||karyopherin Kap113|Schizosaccharomyces pomb...    27   1.8  
SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosacc...    26   4.2  
SPAC3H8.09c |nab3||poly|Schizosaccharomyces pombe|chr 1|||Manual       26   4.2  
SPAC27E2.01 |||alpha-amylase homolog |Schizosaccharomyces pombe|...    25   7.4  
SPAC2C4.06c |||rRNA methyltransferase |Schizosaccharomyces pombe...    25   7.4  
SPBC543.03c |pku80||Ku domain protein Pku80|Schizosaccharomyces ...    25   9.8  
SPAC12G12.07c |||conserved fungal protein|Schizosaccharomyces po...    25   9.8  
SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c...    25   9.8  

>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 492

 Score =  115 bits (276), Expect = 5e-27
 Identities = 62/153 (40%), Positives = 86/153 (56%), Gaps = 2/153 (1%)
 Frame = +2

Query: 101 ARLIALEQDMAKYKPTTDELSANAVEEFVQSFFAGTLKQHLLSEDLPSDWAAKPVKTLVA 280
           A +IA  + M KY   T EL+A A+ +FV  F  G L+  + S+ +P   + + +  LVA
Sbjct: 305 AFVIANLKSMLKYPFPTTELTAKAMTKFVGDFVDGKLQPKIKSQPIPE--SQEDLVVLVA 362

Query: 281 TNFDEIVFDTNKKVLVEFYAPWCGHCKQLVPIYDKLGEHFEXXXXXXXXXXXXTANELEH 460
            NFD+IV D  K VLVEFYAPWCGHCK L P Y+KL E +             T N++  
Sbjct: 363 DNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDDSNVVVAKIDATENDIS- 421

Query: 461 TKITSFPTIKLYTKDNQVRD--YHGERTLAGLT 553
             I+ FPTI  +  +++V    Y G+RTL  L+
Sbjct: 422 VSISGFPTIMFFKANDKVNPVRYEGDRTLEDLS 454



 Score = 56.8 bits (131), Expect = 2e-09
 Identities = 24/76 (31%), Positives = 36/76 (47%), Gaps = 1/76 (1%)
 Frame = +2

Query: 311 NKKVLVEFYAPWCGHCKQLVPIYDKLGEHFEXXXXXXXXXXXXTANEL-EHTKITSFPTI 487
           +K ++V+FYAPWCGHCK L P Y+   +  E               +L     I  +PT+
Sbjct: 39  DKVLMVKFYAPWCGHCKALAPEYESAADELEKDGISLVEVDCTEEGDLCSEYSIRGYPTL 98

Query: 488 KLYTKDNQVRDYHGER 535
            ++    Q+  Y G R
Sbjct: 99  NVFKNGKQISQYSGPR 114


>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 359

 Score = 76.2 bits (179), Expect = 3e-15
 Identities = 41/100 (41%), Positives = 57/100 (57%), Gaps = 4/100 (4%)
 Frame = +2

Query: 263 VKTLVATNFDEIVFDTNKKVLVEFYAPWCGHCKQLVPIYDKLGEHF--EXXXXXXXXXXX 436
           V  L + NFD++V D  K VLVEFYA WCG+CK+L P Y+ LG+ F  E           
Sbjct: 142 VVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTYETLGKVFKNEPNVEIVKINAD 201

Query: 437 XTANELEHTKITSFPTIKLYTKDNQVRD--YHGERTLAGL 550
             A+     ++ SFPTIK + KD++ +   Y G+R+L  L
Sbjct: 202 VFADIGRLHEVASFPTIKFFPKDDKDKPELYEGDRSLESL 241



 Score = 56.0 bits (129), Expect = 3e-09
 Identities = 29/85 (34%), Positives = 41/85 (48%), Gaps = 3/85 (3%)
 Frame = +2

Query: 308 TNKKVLVEFYAPWCGHCKQLVPIYDKLGEHFEXXXXXXXXXXXXTANE--LEHTKITSFP 481
           + K  L+EFYA WCGHCK L P+Y++LG  FE              +    +   IT FP
Sbjct: 38  SKKGALIEFYATWCGHCKSLAPVYEELGALFEDHNDVLIGKIDADTHSDVADKYHITGFP 97

Query: 482 TIKLYTKD-NQVRDYHGERTLAGLT 553
           T+  +  D ++   Y   R +  LT
Sbjct: 98  TLIWFPPDGSEPVQYSNARDVDSLT 122


>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 363

 Score = 53.6 bits (123), Expect = 2e-08
 Identities = 30/83 (36%), Positives = 41/83 (49%), Gaps = 7/83 (8%)
 Frame = +2

Query: 323 LVEFYAPWCGHCKQLVPIYDKLGEHFEXXXXXXXXXXXXTANE--LEHTKITSFPTIKLY 496
           LV FYAPWCG+CK+LVP Y KL  +                N       ++  FPTIKL 
Sbjct: 52  LVVFYAPWCGYCKKLVPTYQKLASNLHSLLPVTAVDCDADQNRAVCSQYQVQGFPTIKLV 111

Query: 497 ---TKDNQV--RDYHGERTLAGL 550
              +K + +   DY+G+R+   L
Sbjct: 112 YPSSKGSSLSSTDYNGDRSYKSL 134


>SPBC12D12.07c |trx2||mitochondrial thioredoxin
           Trx2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 121

 Score = 40.7 bits (91), Expect = 1e-04
 Identities = 19/68 (27%), Positives = 33/68 (48%)
 Frame = +2

Query: 311 NKKVLVEFYAPWCGHCKQLVPIYDKLGEHFEXXXXXXXXXXXXTANELEHTKITSFPTIK 490
           +K  +V+FYA WCG CK L P  +KL E  +             ++  +   + + PT+ 
Sbjct: 35  DKVTVVDFYADWCGPCKYLKPFLEKLSEQNQKASFIAVNADKF-SDIAQKNGVYALPTMV 93

Query: 491 LYTKDNQV 514
           L+ K  ++
Sbjct: 94  LFRKGQEL 101


>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 103

 Score = 36.3 bits (80), Expect = 0.003
 Identities = 19/84 (22%), Positives = 37/84 (44%)
 Frame = +2

Query: 278 ATNFDEIVFDTNKKVLVEFYAPWCGHCKQLVPIYDKLGEHFEXXXXXXXXXXXXTANELE 457
           ++ F  IV   +K V+V+F+A WCG CK + P +++    +             +    E
Sbjct: 8   SSEFKSIVCQ-DKLVVVDFFATWCGPCKAIAPKFEQFSNTYSDATFIKVDVDQLSEIAAE 66

Query: 458 HTKITSFPTIKLYTKDNQVRDYHG 529
              + + P+  LY    ++ +  G
Sbjct: 67  -AGVHAMPSFFLYKNGEKIEEIVG 89


>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 244

 Score = 34.7 bits (76), Expect = 0.009
 Identities = 14/45 (31%), Positives = 26/45 (57%), Gaps = 1/45 (2%)
 Frame = +2

Query: 263 VKTLVATNFDEIVFDTNKKV-LVEFYAPWCGHCKQLVPIYDKLGE 394
           V+      F EI+ +  +++ L+ FYAPW   CKQ+  ++D+  +
Sbjct: 3   VEITFVEQFQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAK 47


>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
           Txl1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 290

 Score = 32.3 bits (70), Expect = 0.049
 Identities = 10/25 (40%), Positives = 16/25 (64%)
 Frame = +2

Query: 326 VEFYAPWCGHCKQLVPIYDKLGEHF 400
           V+ YA WCG CK + P++ +L   +
Sbjct: 24  VDCYADWCGPCKAISPLFSQLASKY 48


>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 726

 Score = 30.7 bits (66), Expect = 0.15
 Identities = 10/23 (43%), Positives = 17/23 (73%)
 Frame = +2

Query: 326 VEFYAPWCGHCKQLVPIYDKLGE 394
           +++Y P CG CK+L P++D + E
Sbjct: 47  IKYYLPSCGACKRLGPMWDNMVE 69


>SPAC17G8.03c |dpb3||DNA polymerase epsilon subunit
           Dpb3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 199

 Score = 27.1 bits (57), Expect = 1.8
 Identities = 16/53 (30%), Positives = 26/53 (49%), Gaps = 3/53 (5%)
 Frame = +2

Query: 83  KEEVPAARL---IALEQDMAKYKPTTDELSANAVEEFVQSFFAGTLKQHLLSE 232
           K   P AR+   +  +QD+ K    T  + + A+E F+QS    + KQ  L +
Sbjct: 21  KSRFPVARIKKIMQADQDVGKVAQVTPVIMSKALELFMQSIIQESCKQTRLHQ 73


>SPCC1322.06 |kap113||karyopherin Kap113|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 983

 Score = 27.1 bits (57), Expect = 1.8
 Identities = 9/30 (30%), Positives = 20/30 (66%)
 Frame = -1

Query: 130 HVLFESDQSCRWNFFLLHAEEL*DSLVFIL 41
           HV+FE+D    W+ FL++ ++L ++   ++
Sbjct: 678 HVIFEADAMELWSTFLMYIQKLPETFTLLI 707


>SPCC132.04c |||NAD-dependent glutamate dehydrogenase
            |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1106

 Score = 25.8 bits (54), Expect = 4.2
 Identities = 14/45 (31%), Positives = 20/45 (44%)
 Frame = +2

Query: 263  VKTLVATNFDEIVFDTNKKVLVEFYAPWCGHCKQLVPIYDKLGEH 397
            V T  A   +E+     +   +EF A W GH +  +P Y  L  H
Sbjct: 969  VPTFYADYVNEVKRIIQRNANLEFEAIWKGHSENKIP-YTSLSNH 1012


>SPAC3H8.09c |nab3||poly|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 738

 Score = 25.8 bits (54), Expect = 4.2
 Identities = 14/58 (24%), Positives = 29/58 (50%)
 Frame = +2

Query: 152 DELSANAVEEFVQSFFAGTLKQHLLSEDLPSDWAAKPVKTLVATNFDEIVFDTNKKVL 325
           ++ + N+  + +Q +   T  +HL  + L +     P KT  + NF+EI +  + + L
Sbjct: 187 EQETENSSTKDLQVYDFQTASEHLPEQSLQNTTYYDPSKTYSSVNFEEIEYGKSHEKL 244


>SPAC27E2.01 |||alpha-amylase homolog |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 491

 Score = 25.0 bits (52), Expect = 7.4
 Identities = 12/29 (41%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
 Frame = +2

Query: 263 VKTLVAT-NFDEIVFDTNKKVLVEFYAPW 346
           ++ L+ T +FD +  DT K V  EFY P+
Sbjct: 206 IRDLIQTYHFDGLRIDTAKHVQKEFYPPF 234


>SPAC2C4.06c |||rRNA methyltransferase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 455

 Score = 25.0 bits (52), Expect = 7.4
 Identities = 20/69 (28%), Positives = 30/69 (43%)
 Frame = +2

Query: 71  FGMKKEEVPAARLIALEQDMAKYKPTTDELSANAVEEFVQSFFAGTLKQHLLSEDLPSDW 250
           F  KK +    R  AL  +  KYKP  DE+ A + + F++   A  L   LL        
Sbjct: 26  FNSKKHD--PKRTYALVCETLKYKPVLDEIIARS-DCFLKENLARVLVHDLLMSKRGLSI 82

Query: 251 AAKPVKTLV 277
           +  P+K  +
Sbjct: 83  SNGPIKECI 91


>SPBC543.03c |pku80||Ku domain protein Pku80|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 695

 Score = 24.6 bits (51), Expect = 9.8
 Identities = 14/45 (31%), Positives = 25/45 (55%), Gaps = 3/45 (6%)
 Frame = -1

Query: 250 PVRGQVLAQQVLFQSS---SEERLHEFLNSVSAKLIGGRFVLSHV 125
           PV+ +  +Q+  F +    SEE++ E LNS   +  G + ++ HV
Sbjct: 541 PVKPKYSSQETAFDTGAPISEEQIEELLNSGLDEQEGEKLLVLHV 585


>SPAC12G12.07c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 412

 Score = 24.6 bits (51), Expect = 9.8
 Identities = 17/68 (25%), Positives = 28/68 (41%)
 Frame = +2

Query: 59  ILEFFGMKKEEVPAARLIALEQDMAKYKPTTDELSANAVEEFVQSFFAGTLKQHLLSEDL 238
           ++ + G +K E   A L     D+        E+S   +   V +FFA  L      E +
Sbjct: 159 VIVYEGTEKSEKAVADLNDSSTDVVP----ESEVSFQTISSRVDNFFAAPLPSEQAEELI 214

Query: 239 PSDWAAKP 262
             D+A +P
Sbjct: 215 EDDYAEQP 222


>SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 807

 Score = 24.6 bits (51), Expect = 9.8
 Identities = 13/37 (35%), Positives = 18/37 (48%)
 Frame = -2

Query: 297 ISSKLVATKVLTGLAAQSEGRSSLNKCCFRVPAKNDC 187
           +  KLV +K    L  ++  + SL KC F     NDC
Sbjct: 457 LKPKLVISKSFGTLIVKNHNQLSLLKCTFSNLDGNDC 493


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,979,292
Number of Sequences: 5004
Number of extensions: 35433
Number of successful extensions: 125
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 119
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 123
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 229961028
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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