Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0005_L03
(677 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|... 204 7e-54
SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces pombe... 198 6e-52
SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosacc... 153 2e-38
SPCC550.10 |meu8||betaine aldehyde dehydrogenase |Schizosaccharo... 150 2e-37
SPAC139.05 |||succinate-semialdehyde dehydrogenase |Schizosaccha... 148 6e-37
SPBC24C6.04 |||delta-1-pyrroline-5-carboxylate dehydrogenase|Sch... 57 2e-09
SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces pomb... 52 8e-08
SPAC31G5.01 |sap49|SPAPB1A11.05|RNA-binding protein Sap49|Schizo... 27 1.9
SPBC543.03c |pku80||Ku domain protein Pku80|Schizosaccharomyces ... 27 2.5
SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase ... 25 7.6
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 25 7.6
>SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 503
Score = 204 bits (499), Expect = 7e-54
Identities = 99/216 (45%), Positives = 133/216 (61%)
Frame = +1
Query: 28 NPQDESVIVQVAEGXXXXXXXXXXXXXXXFHRYSEWRLLDASQRGLLLFKLATLMERDAK 207
+P E +I +VA+ F + WR ++QRG L +LA +E++ +
Sbjct: 44 SPSTEKLICEVADADEEDVDIAVKVARAAFQTDAPWRKFSSAQRGRCLSRLADCIEQNLE 103
Query: 208 YLAELETLDNGKPVKQAEQEVLWASGIVRYYAGKADKILGNTIPADGEVLTFTLKEPVGV 387
YLA +ETLDNGK + A +V A+ RYY G ADK G TI D + +T EP+GV
Sbjct: 104 YLASIETLDNGKSITLARGDVQAAADCFRYYGGWADKDYGQTIETDIKRFAYTRHEPIGV 163
Query: 388 CGQILPWNYPIPMFIWKIAPALAAGCTVVVKPAEQTPLTALAMAALVKEAGVPAGVVNVI 567
CGQI+PWN+P M WKIAPA+A G T+++K AE TPL+AL + V E G P GV+NV+
Sbjct: 164 CGQIIPWNFPFLMCAWKIAPAVACGNTIILKTAELTPLSALCLTKFVPECGFPPGVINVL 223
Query: 568 PGYGPTAGAALTLHPDVDXVAFTGSTEVGRIIMRAA 675
G G G A++ H D+D VAFTGST VGR++MRAA
Sbjct: 224 SGDGRRCGNAISSHMDIDKVAFTGSTGVGRMVMRAA 259
>SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 496
Score = 198 bits (483), Expect = 6e-52
Identities = 102/225 (45%), Positives = 136/225 (60%), Gaps = 1/225 (0%)
Frame = +1
Query: 4 SKKTFPTINPQDESVIVQVAEGXXXXXXXXXXXXXXXFHRYSEWRLLDASQRGLLLFKLA 183
S +T+ T++P E VI +V F W+ + S++G LL KLA
Sbjct: 32 SAETWETVDPSIEEVIAKVYLAGEKEIDYAVKSAKEAF---KTWKKVPGSEKGELLMKLA 88
Query: 184 TLMERDAKYLAELETLDNGKP-VKQAEQEVLWASGIVRYYAGKADKILGNTIPADGEVLT 360
L E+ A LA +E +D+GKP V A +V ++RY AG ADKI G IP E L
Sbjct: 89 ELTEKHADTLAAIEAMDSGKPLVSNARGDVDGTIALLRYCAGWADKIYGQVIPTGPEKLA 148
Query: 361 FTLKEPVGVCGQILPWNYPIPMFIWKIAPALAAGCTVVVKPAEQTPLTALAMAALVKEAG 540
+ + P+GVCGQI+PWNYP+ M WKIAPALAAG +++K AE TPL+ L A LV+EAG
Sbjct: 149 YAKRTPIGVCGQIVPWNYPLNMAGWKIAPALAAGNCIIIKSAETTPLSLLYFATLVEEAG 208
Query: 541 VPAGVVNVIPGYGPTAGAALTLHPDVDXVAFTGSTEVGRIIMRAA 675
P GVVN+I G G AG+ + HP +D +AFTGST+VG I+ + A
Sbjct: 209 FPKGVVNIISGLGTVAGSYMAKHPGIDKIAFTGSTKVGVIVQQLA 253
>SPAC1002.12c |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 498
Score = 153 bits (371), Expect = 2e-38
Identities = 86/224 (38%), Positives = 130/224 (58%), Gaps = 2/224 (0%)
Frame = +1
Query: 4 SKKTFPTINPQDESVIVQVAEGXXXXXXXXXXXXXXXFHRYSEWRLLDASQRGLLLFKLA 183
S KTF NP + V + F Y R D +R +L +
Sbjct: 37 SGKTFDVENPGLNETLAPVTDMSVEETRKAIKVAHEAFLSY---RNSDIKERYAILRRWY 93
Query: 184 TLMERDAKYLAELETLDNGKPVKQAEQEVLWASGIVRYYAGKADKILGNT-IPADGEVLT 360
L+ +A LA + TL+NGK + A+ EV++A+ + ++AG+A +I G++ + ++ +
Sbjct: 94 DLIMENADDLATMMTLENGKALGDAKGEVVYAAKFIDWFAGEALRISGDSSMSSNPQNRI 153
Query: 361 FTLKEPVGVCGQILPWNYPIPMFIWKIAPALAAGCTVVVKPAEQTPLTALAMAALVKEAG 540
T+K+PVGV G I PWN+P M K+ ALAAGCTVV++PA +TP TALA+A L + AG
Sbjct: 154 ITIKQPVGVVGIITPWNFPAAMITRKVGAALAAGCTVVIRPAAETPFTALALAKLAERAG 213
Query: 541 VPAGVVNVIPGYGPTA-GAALTLHPDVDXVAFTGSTEVGRIIMR 669
VPAGV+N++ P+ G LT +P + V+FTGST VG+I+ +
Sbjct: 214 VPAGVLNMVTANSPSEHGIELTTNPLIRKVSFTGSTNVGKILAK 257
>SPCC550.10 |meu8||betaine aldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 500
Score = 150 bits (364), Expect = 2e-37
Identities = 84/225 (37%), Positives = 119/225 (52%), Gaps = 3/225 (1%)
Frame = +1
Query: 10 KTFPTINPQDESVIVQVAEGXXXXXXXXXXXXXXXFHRYSEWRLLDASQRGLLLFKLATL 189
K P INP E +I A F R W QRGL+L K+A +
Sbjct: 38 KPIPLINPATEEIIGTCANASAKDVDSAVENAYNTF-RSGIWAKWPGKQRGLVLRKIAKM 96
Query: 190 MERDAKYLAELETLDNGKPVKQAEQEVLWASGIVRYYAGKADK---ILGNTIPADGEVLT 360
M + LA ++T++ GKP A ++ + + YYA A+ + +P +
Sbjct: 97 MREKRELLAGIDTINCGKPTPYALFDIDSCADMFEYYAEVAETDNPTVKVPLPNNPGFCA 156
Query: 361 FTLKEPVGVCGQILPWNYPIPMFIWKIAPALAAGCTVVVKPAEQTPLTALAMAALVKEAG 540
F + P GV G I PWN+P+ M +WK+ PA+A+G VV+KP+E P + L A + KEAG
Sbjct: 157 FEKRFPRGVIGVITPWNFPLKMALWKLVPAIASGNCVVLKPSELAPWSCLEFALICKEAG 216
Query: 541 VPAGVVNVIPGYGPTAGAALTLHPDVDXVAFTGSTEVGRIIMRAA 675
+P GV+NVI G G +GAAL+ HP + +AFTGS G+ IM AA
Sbjct: 217 LPDGVLNVIIGSGKESGAALSCHPKIAYLAFTGSLATGKKIMHAA 261
>SPAC139.05 |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 493
Score = 148 bits (359), Expect = 6e-37
Identities = 86/224 (38%), Positives = 121/224 (54%), Gaps = 3/224 (1%)
Frame = +1
Query: 4 SKKTFPTINPQDESVIVQVAEGXXXXXXXXXXXXXXXFHRYSEWRLLDASQRGLLLFKLA 183
+ KTF NP +I +VA+ F Y + + SQ LL + A
Sbjct: 31 NNKTFEVDNPATGEIIGKVADVSVEETKKAISAANEAFKTYKNFTHVQRSQ---LLERWA 87
Query: 184 TLMERDAKYLAELETLDNGKPVKQAEQEVLWASGIVRYYAGKADKILGNTIPADGEVLTF 363
L+ + L ++ TL+NGKP+ QAE EV SG +++YA +A + G+ P+ + F
Sbjct: 88 ELIMENKDDLVKMLTLENGKPLSQAEMEVTTCSGYLKWYAAEAVRTFGDVAPSSLQSQNF 147
Query: 364 --TLKEPVGVCGQILPWNYPIPMFIWKIAPALAAGCTVVVKPAEQTPLTALAMAALVKEA 537
++K+PVGV I PWN+P M K ALAAGCT + PA +TP L + L +EA
Sbjct: 148 LISIKQPVGVSALITPWNFPAAMIARKGGAALAAGCTAIFLPAFRTPYVCLGLVRLAQEA 207
Query: 538 GVPAGVVNVIPGYGPTA-GAALTLHPDVDXVAFTGSTEVGRIIM 666
G P GV+NVI +A G LT +P V V+FTGST VG+I+M
Sbjct: 208 GFPDGVLNVITSSDASAHGKELTTNPIVRKVSFTGSTNVGKILM 251
>SPBC24C6.04 |||delta-1-pyrroline-5-carboxylate
dehydrogenase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 548
Score = 57.2 bits (132), Expect = 2e-09
Identities = 47/179 (26%), Positives = 80/179 (44%), Gaps = 4/179 (2%)
Frame = +1
Query: 133 WRLLDASQRGLLLFKLATLMERDAKY-LAELETLDNGKPVKQAEQEV-LWASGIVRYYAG 306
W L + R + K A L+ +Y L + GK + QAE + + +R+
Sbjct: 97 WESLPFADRSAIFLKAAHLISTKYRYKLMAATMIGQGKNIWQAEIDAGMEIIDFLRFNTK 156
Query: 307 KADKILGNTIPADGEVLTFTLK-EPV-GVCGQILPWNYPIPMFIWKIAPALAAGCTVVVK 480
A ++ + P + + ++ P+ G I P+N+ AP L G V++K
Sbjct: 157 YASELYASQPPENTPGVWNRMEYRPLEGFVYAITPFNFTAIAGNLAAAPLLM-GNVVLMK 215
Query: 481 PAEQTPLTALAMAALVKEAGVPAGVVNVIPGYGPTAGAALTLHPDVDXVAFTGSTEVGR 657
P++ L++ + + +EAG+PAG + IPG HP+ + FTGST V R
Sbjct: 216 PSDHAVLSSYIVYQIFREAGLPAGALQFIPGDAVEVSKVCFNHPEFAGLHFTGSTAVFR 274
>SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 522
Score = 52.0 bits (119), Expect = 8e-08
Identities = 49/192 (25%), Positives = 82/192 (42%), Gaps = 10/192 (5%)
Frame = +1
Query: 130 EWRLLDASQRGLLLFKLATLMERDAKYLAELETLDNGKP-VKQAEQEVLWASGIVRYYAG 306
EW+ ++R L L + R+ AE+ D GK V A E+L + +
Sbjct: 40 EWKSTSFAERRNFLKALKENIIRNQDKYAEIACKDTGKTLVDAAFGEILVTLEKINWTLA 99
Query: 307 KADKILGNTIPADGEVLT-----FTLKEPVGVCGQILPWNYPIPMFIWKIAPALAAGCTV 471
++ L T +LT + EP+GV ++ WNYP+ + I AL AG +
Sbjct: 100 NGEQSLRPT-KRPNSLLTSYKGGYVKYEPLGVIAALVSWNYPLHNALGPIISALFAGNAI 158
Query: 472 VVKPAEQTPLT----ALAMAALVKEAGVPAGVVNVIPGYGPTAGAALTLHPDVDXVAFTG 639
VVK +E T + + +L++ G +V I P LT H + + F G
Sbjct: 159 VVKGSELTAWSTHQYCEMVRSLLQSMGHSPELVQCITCL-PDVADHLTSHSGIKHITFIG 217
Query: 640 STEVGRIIMRAA 675
S + +++ +A
Sbjct: 218 SQPIAKLVAASA 229
>SPAC31G5.01 |sap49|SPAPB1A11.05|RNA-binding protein
Sap49|Schizosaccharomyces pombe|chr 1|||Manual
Length = 335
Score = 27.5 bits (58), Expect = 1.9
Identities = 18/42 (42%), Positives = 21/42 (50%), Gaps = 4/42 (9%)
Frame = -1
Query: 572 PGITLTTPAGTPASLTSAAIASA----VSGVCSAGLTTTVQP 459
PG + TPA T A+ T A IA+ V V G TT V P
Sbjct: 212 PGFSPATPAPTSAANTPATIAATSIPPVPNVPLVGATTAVPP 253
>SPBC543.03c |pku80||Ku domain protein Pku80|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 695
Score = 27.1 bits (57), Expect = 2.5
Identities = 22/77 (28%), Positives = 35/77 (45%), Gaps = 2/77 (2%)
Frame = +1
Query: 163 LLLFKLATLMERDAKYLAELETLDNGKPVKQAEQEVLWASGIVRYYAGKADKILGNTIPA 342
L+ KL + KY ++ D G P+ + + E L SG+ + +K+L +
Sbjct: 531 LIKEKLTVNVPVKPKYSSQETAFDTGAPISEEQIEELLNSGLDEQ---EGEKLLVLHVSE 587
Query: 343 DGEVLTFT--LKEPVGV 387
V TFT LK P G+
Sbjct: 588 KDPVGTFTEVLKNPFGL 604
>SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 423
Score = 25.4 bits (53), Expect = 7.6
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = +1
Query: 247 VKQAEQEVLWASGIVRYYAGKADKILG 327
VK+ + +W G + YAG+ +ILG
Sbjct: 219 VKKGDTVAIWGMGPIGLYAGRWAQILG 245
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.4 bits (53), Expect = 7.6
Identities = 10/26 (38%), Positives = 14/26 (53%), Gaps = 4/26 (15%)
Frame = +3
Query: 498 THSACYGRACQGSWCPSR----SCQC 563
T+ CY C+ CPS+ SC+C
Sbjct: 399 TNGGCYSYICRSRSCPSKYQCYSCRC 424
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,603,820
Number of Sequences: 5004
Number of extensions: 52675
Number of successful extensions: 150
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 130
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 143
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 311890690
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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