Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0005_K17
(449 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g50950.2 68418.m06319 fumarate hydratase, putative / fumarase... 234 2e-62
At5g50950.1 68418.m06318 fumarate hydratase, putative / fumarase... 234 2e-62
At2g47510.1 68415.m05930 fumarate hydratase, putative / fumarase... 234 2e-62
At5g27630.1 68418.m03310 acyl-CoA binding family protein similar... 31 0.48
At5g10920.1 68418.m01267 argininosuccinate lyase, putative / arg... 29 1.9
At4g27340.1 68417.m03923 Met-10+ like family protein ; contains... 28 2.5
At5g43930.1 68418.m05374 transducin family protein / WD-40 repea... 27 4.4
At3g05420.2 68416.m00594 acyl-CoA binding family protein similar... 27 5.9
At3g05420.1 68416.m00593 acyl-CoA binding family protein similar... 27 5.9
At5g46250.2 68418.m05694 RNA recognition motif (RRM)-containing ... 27 7.7
At5g46250.1 68418.m05693 RNA recognition motif (RRM)-containing ... 27 7.7
At4g17550.1 68417.m02624 transporter-related similar to cAMP ind... 27 7.7
At4g16530.1 68417.m02502 expressed protein contains Pfam profile... 27 7.7
At3g11900.1 68416.m01459 amino acid transporter family protein l... 27 7.7
At1g52360.1 68414.m05909 coatomer protein complex, subunit beta ... 27 7.7
>At5g50950.2 68418.m06319 fumarate hydratase, putative / fumarase,
putative similar to SP|P55250 Fumarate hydratase,
mitochondrial precursor (EC 4.2.1.2) (Fumarase)
{Rhizopus oryzae}; contains Pfam profile PF00206: Lyase
Length = 499
Score = 234 bits (572), Expect = 2e-62
Identities = 109/149 (73%), Positives = 123/149 (82%)
Frame = +2
Query: 2 PFESAPNKFEALACHDAMVEVSGALNTIAVSLMKIGNDIRLLASGPRCGLAELMLPENEP 181
PF +A NKFEALA HDA VE SG+LNTIA SLMKI NDIR L SGPRCGL EL LPENEP
Sbjct: 292 PFVTAENKFEALAAHDACVETSGSLNTIATSLMKIANDIRFLGSGPRCGLGELSLPENEP 351
Query: 182 GSSIMPGKVNPTQCEALTMIAVQVMGNHVGCTIGGSNGHFELNVFKPMMVANVLRSIRLI 361
GSSIMPGKVNPTQCEALTM+ QVMGNHV TIGGSNGHFELNVFKP++ + +L SIRLI
Sbjct: 352 GSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTIGGSNGHFELNVFKPVIASALLHSIRLI 411
Query: 362 GDRSSAFNKNCAVGIKANEAQIAKIMRES 448
D S++F KNC GI+AN +I+K++ ES
Sbjct: 412 ADASASFEKNCVRGIEANRERISKLLHES 440
>At5g50950.1 68418.m06318 fumarate hydratase, putative / fumarase,
putative similar to SP|P55250 Fumarate hydratase,
mitochondrial precursor (EC 4.2.1.2) (Fumarase)
{Rhizopus oryzae}; contains Pfam profile PF00206: Lyase
Length = 510
Score = 234 bits (572), Expect = 2e-62
Identities = 109/149 (73%), Positives = 123/149 (82%)
Frame = +2
Query: 2 PFESAPNKFEALACHDAMVEVSGALNTIAVSLMKIGNDIRLLASGPRCGLAELMLPENEP 181
PF +A NKFEALA HDA VE SG+LNTIA SLMKI NDIR L SGPRCGL EL LPENEP
Sbjct: 292 PFVTAENKFEALAAHDACVETSGSLNTIATSLMKIANDIRFLGSGPRCGLGELSLPENEP 351
Query: 182 GSSIMPGKVNPTQCEALTMIAVQVMGNHVGCTIGGSNGHFELNVFKPMMVANVLRSIRLI 361
GSSIMPGKVNPTQCEALTM+ QVMGNHV TIGGSNGHFELNVFKP++ + +L SIRLI
Sbjct: 352 GSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTIGGSNGHFELNVFKPVIASALLHSIRLI 411
Query: 362 GDRSSAFNKNCAVGIKANEAQIAKIMRES 448
D S++F KNC GI+AN +I+K++ ES
Sbjct: 412 ADASASFEKNCVRGIEANRERISKLLHES 440
>At2g47510.1 68415.m05930 fumarate hydratase, putative / fumarase,
putative similar to SP|P55250 Fumarate hydratase,
mitochondrial precursor (EC 4.2.1.2) (Fumarase)
{Rhizopus oryzae}; contains Pfam profile PF00206: Lyase
Length = 492
Score = 234 bits (572), Expect = 2e-62
Identities = 107/149 (71%), Positives = 124/149 (83%)
Frame = +2
Query: 2 PFESAPNKFEALACHDAMVEVSGALNTIAVSLMKIGNDIRLLASGPRCGLAELMLPENEP 181
PF +A NKFEALA HDA VE SG+LNTIA SLMKI NDIR L SGPRCGL EL+LPENEP
Sbjct: 285 PFVTAENKFEALAAHDACVETSGSLNTIATSLMKIANDIRFLGSGPRCGLGELVLPENEP 344
Query: 182 GSSIMPGKVNPTQCEALTMIAVQVMGNHVGCTIGGSNGHFELNVFKPMMVANVLRSIRLI 361
GSSIMPGKVNPTQCEALTM+ QVMGNHV T+GGSNGHFELNVFKP++ + +L S+RLI
Sbjct: 345 GSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPVIASALLHSVRLI 404
Query: 362 GDRSSAFNKNCAVGIKANEAQIAKIMRES 448
D S++F KNC GI+AN +I+K++ ES
Sbjct: 405 ADASASFEKNCVRGIEANRERISKLLHES 433
>At5g27630.1 68418.m03310 acyl-CoA binding family protein similar to
RING finger rngB protein, cytosolic - Dictyostelium
discoideum, PIR:S68824; contains Pfam profiles PF01344:
Kelch motif, PF00887: Acyl CoA binding protein (ACBP)
Length = 648
Score = 30.7 bits (66), Expect = 0.48
Identities = 14/39 (35%), Positives = 20/39 (51%), Gaps = 2/39 (5%)
Frame = +2
Query: 212 PTQCEALTMIAVQVMGNHVGCTIGGSNGHF--ELNVFKP 322
P E L+++ G + GG NGH+ E+NV KP
Sbjct: 446 PLASEGLSLVVSSYNGEDIVVAFGGYNGHYNNEVNVLKP 484
>At5g10920.1 68418.m01267 argininosuccinate lyase, putative /
arginosuccinase, putative similar to argininosuccinate
lyase [Nostoc punctiforme] GI:7672743; contains Pfam
profile PF00206: Lyase
Length = 517
Score = 28.7 bits (61), Expect = 1.9
Identities = 26/89 (29%), Positives = 41/89 (46%), Gaps = 2/89 (2%)
Frame = +2
Query: 8 ESAPNKFEALACHDAMVEVSGALNTIAVSLMKIGNDIRLLASGPRCGLAELMLPENE--P 181
E N +A++ D ++E + L ++G + L AS G M P +
Sbjct: 276 EPMRNSIDAVSDRDFVLEFLYTNANTGIHLSRLGEEWVLWAS-EEFGF---MTPSDSVST 331
Query: 182 GSSIMPGKVNPTQCEALTMIAVQVMGNHV 268
GSSIMP K NP E + + +V+G+ V
Sbjct: 332 GSSIMPQKKNPDPMELVRGKSARVIGDLV 360
>At4g27340.1 68417.m03923 Met-10+ like family protein ; contains
Pfam profile PF02475: Met-10+ like-protein
Length = 619
Score = 28.3 bits (60), Expect = 2.5
Identities = 14/49 (28%), Positives = 26/49 (53%)
Frame = +2
Query: 149 LAELMLPENEPGSSIMPGKVNPTQCEALTMIAVQVMGNHVGCTIGGSNG 295
+A+++L +N+P + K++P + TM + GNH T+ NG
Sbjct: 366 IAKVVLDKNQPKIQTVVNKIDPIHNDFRTMQLEVLAGNHSLVTLVVENG 414
>At5g43930.1 68418.m05374 transducin family protein / WD-40 repeat
family protein contains 4 WD-40 repeats (PF00400);
similar to WD-repeat protein 5 (SP:Q9UGP9) [Homo
sapiens]
Length = 726
Score = 27.5 bits (58), Expect = 4.4
Identities = 25/79 (31%), Positives = 34/79 (43%), Gaps = 1/79 (1%)
Frame = +2
Query: 11 SAPNKFEALACHDAMVEVSGALNTIAVSLMKIGNDIRLLASGPRCGLAELMLPEN-EPGS 187
SAP+ +L A++E+ +N AVS + R GL E + N + GS
Sbjct: 417 SAPSSTASLEAAVALLEIPSGVNLHAVSRRGGAQEQTSQPQFSRTGLPEGVSSRNTQHGS 476
Query: 188 SIMPGKVNPTQCEALTMIA 244
P VN Q E T IA
Sbjct: 477 DAQP-VVNRVQSELATSIA 494
>At3g05420.2 68416.m00594 acyl-CoA binding family protein similar to
PIR|S68824|S68824 rngB protein, cytosolic (Dictyostelium
discoideum); contains Pfam profiles PF00887: Acyl CoA
binding protein, PF01344: Kelch motif
Length = 669
Score = 27.1 bits (57), Expect = 5.9
Identities = 15/43 (34%), Positives = 22/43 (51%), Gaps = 2/43 (4%)
Frame = +2
Query: 200 GKVNPTQCEALTMIAVQVMGNHVGCTIGGSNGHF--ELNVFKP 322
G+V P E L+++ G V GG NG + E+N+ KP
Sbjct: 443 GRV-PLASEGLSLVVSSYNGEDVLVAFGGYNGRYNNEINLLKP 484
>At3g05420.1 68416.m00593 acyl-CoA binding family protein similar to
PIR|S68824|S68824 rngB protein, cytosolic (Dictyostelium
discoideum); contains Pfam profiles PF00887: Acyl CoA
binding protein, PF01344: Kelch motif
Length = 668
Score = 27.1 bits (57), Expect = 5.9
Identities = 15/43 (34%), Positives = 22/43 (51%), Gaps = 2/43 (4%)
Frame = +2
Query: 200 GKVNPTQCEALTMIAVQVMGNHVGCTIGGSNGHF--ELNVFKP 322
G+V P E L+++ G V GG NG + E+N+ KP
Sbjct: 442 GRV-PLASEGLSLVVSSYNGEDVLVAFGGYNGRYNNEINLLKP 483
>At5g46250.2 68418.m05694 RNA recognition motif (RRM)-containing
protein contains similarity to RNA-binding protein;
contains InterPro entry IPR000504: RNA-binding region
RNP-1 (RNA recognition motif) (RRM)
Length = 420
Score = 26.6 bits (56), Expect = 7.7
Identities = 13/31 (41%), Positives = 19/31 (61%)
Frame = -3
Query: 405 IPTAQFLLNADDRSPMSLMERSTLATIIGLK 313
+PT +FLLNA R+ + ST+AT +K
Sbjct: 120 LPTDKFLLNAMKRNKKGFVPISTIATFHKMK 150
>At5g46250.1 68418.m05693 RNA recognition motif (RRM)-containing
protein contains similarity to RNA-binding protein;
contains InterPro entry IPR000504: RNA-binding region
RNP-1 (RNA recognition motif) (RRM)
Length = 422
Score = 26.6 bits (56), Expect = 7.7
Identities = 13/31 (41%), Positives = 19/31 (61%)
Frame = -3
Query: 405 IPTAQFLLNADDRSPMSLMERSTLATIIGLK 313
+PT +FLLNA R+ + ST+AT +K
Sbjct: 120 LPTDKFLLNAMKRNKKGFVPISTIATFHKMK 150
>At4g17550.1 68417.m02624 transporter-related similar to cAMP
inducible 2 protein [Mus musculus] GI:4580997, glycerol
3-phosphate permease [Homo sapiens] GI:7543982; contains
Pfam profile PF00083: major facilitator superfamily
protein
Length = 544
Score = 26.6 bits (56), Expect = 7.7
Identities = 19/52 (36%), Positives = 25/52 (48%)
Frame = -2
Query: 307 QLEVPVAPPYSAADVVAHHLDRDHGQGLALRRIHFTWHDRGSGFVLGQHQLG 152
+++V YS VA HL G L LR + TW GSGF +G +G
Sbjct: 118 EIDVAFLACYSIGMYVAGHL----GDSLDLR-LFLTWGMIGSGFFVGLFGMG 164
>At4g16530.1 68417.m02502 expressed protein contains Pfam profile
PF04510: Family of unknown function (DUF577)
Length = 774
Score = 26.6 bits (56), Expect = 7.7
Identities = 15/44 (34%), Positives = 25/44 (56%), Gaps = 2/44 (4%)
Frame = +1
Query: 199 R*SESDAVRGPDHDRGPSDGQPRRLHYRGEQ--RALRAERLQTD 324
R E+D+VRG D D G + ++ G++ + LR ERL+ +
Sbjct: 706 RNDENDSVRGEDDDGGCGGYEAKKKKMSGKKSLKELREERLKRE 749
>At3g11900.1 68416.m01459 amino acid transporter family protein low
similarity to proton/amino acid transporter 1 [Mus
musculus] GI:21908024; belongs to INTERPRO:IPR002422
amino acid/polyamine transporter, family II
Length = 432
Score = 26.6 bits (56), Expect = 7.7
Identities = 13/26 (50%), Positives = 16/26 (61%), Gaps = 2/26 (7%)
Frame = -3
Query: 261 LPITWTAIMVRASHCVG--FTLPGMI 190
LP W+AI V+ CVG FT P M+
Sbjct: 288 LPNNWSAIAVQIGLCVGLTFTFPIMV 313
>At1g52360.1 68414.m05909 coatomer protein complex, subunit beta 2
(beta prime), putative contains 7 WD-40 repeats
(PF00400) (1 weak); similar to (SP:O55029) Coatomer
beta' subunit (Beta'-coat protein) (Beta'-COP) (p102)
(SP:O55029) [Mus musculus]; similar to GI:298096 from
[Homo sapiens]
Length = 926
Score = 26.6 bits (56), Expect = 7.7
Identities = 12/37 (32%), Positives = 19/37 (51%)
Frame = -2
Query: 409 LDTDGAVLVER*RSVANEPDGAQHVGDHHRFEDVQLE 299
+D + V E NE D A+HV +HH ++ + E
Sbjct: 856 MDHEEVVAEENGNEQRNEDDVAEHVEEHHEEKEAEEE 892
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,980,655
Number of Sequences: 28952
Number of extensions: 176940
Number of successful extensions: 563
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 551
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 563
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 732537840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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