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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0005_J24
         (574 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g25240.1 68415.m03020 serpin, putative / serine protease inhi...    45   3e-05
At3g45220.1 68416.m04880 serpin, putative / serine protease inhi...    45   4e-05
At2g26390.1 68415.m03167 serpin, putative / serine protease inhi...    42   3e-04
At1g47710.1 68414.m05302 serpin, putative / serine protease inhi...    40   0.002
At1g64030.1 68414.m07252 serpin family protein / serine protease...    33   0.18 
At1g62170.1 68414.m07013 serpin family protein / serine protease...    29   2.2  
At1g10610.1 68414.m01202 basic helix-loop-helix (bHLH) family pr...    28   5.1  
At2g35580.1 68415.m04357 serpin family protein / serine protease...    27   6.7  
At4g14310.1 68417.m02204 peroxisomal membrane protein-related co...    27   8.9  
At2g14540.1 68415.m01628 serpin family protein / serine protease...    27   8.9  
At1g64010.1 68414.m07250 serpin, putative / serine protease inhi...    27   8.9  

>At2g25240.1 68415.m03020 serpin, putative / serine protease
           inhibitor, putative similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311; contains Pfam profile
           PF00079: Serpin (serine protease inhibitor)
          Length = 324

 Score = 45.2 bits (102), Expect = 3e-05
 Identities = 33/112 (29%), Positives = 53/112 (47%), Gaps = 6/112 (5%)
 Frame = -1

Query: 376 KIPRFQISTNLVLNKPLNDMGVYDIFQPDVASFQRVTK----ENIFVSAIVHKADIEVTE 209
           +IP+F+ S     ++ L DMG+   F       + V      ++++VS+I+HKA IEV E
Sbjct: 211 RIPKFKFSFEFNASEVLKDMGLTSPFNNGGGLTEMVDSPSNGDDLYVSSILHKACIEVDE 270

Query: 208 XXXXXXXXXXXXXSDRISA--PNFHANRPFVYFVMEKSTLTVLFSGIYSKPT 59
                        S       P+F A+RPF++ V E  +  +LF G    P+
Sbjct: 271 EGTEAAAVSVGVVSCTSFRRNPDFVADRPFLFTVREDKSGVILFMGQVLDPS 322


>At3g45220.1 68416.m04880 serpin, putative / serine protease
           inhibitor, putative similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311; contains Pfam profile
           PF00079: Serpin (serine protease inhibitor)
          Length = 393

 Score = 44.8 bits (101), Expect = 4e-05
 Identities = 44/174 (25%), Positives = 75/174 (43%), Gaps = 12/174 (6%)
 Frame = -1

Query: 541 IPYATDGK-YSMLFVIPYGNVKIQDMYKNFAEVSLKDIFKKLQDDVDKFGLEEIDVKIPR 365
           +PY  D + ++M   +P     +  + +   E+S K  F  L + + +  +     KIP+
Sbjct: 225 LPYVEDQRQFAMYIYLPNDRDGLPTLLE---EISSKPRF--LDNHIPRQRILTEAFKIPK 279

Query: 364 FQISTNLVLNKPLNDMGVYDIF----------QPDVASFQRVTKENIFVSAIVHKADIEV 215
           F+ S     +  L +MG+   F           P +     V  EN+FVS + HKA IEV
Sbjct: 280 FKFSFEFKASDVLKEMGLTLPFTHGSLTEMVESPSIPENLCVA-ENLFVSNVFHKACIEV 338

Query: 214 -TEXXXXXXXXXXXXXSDRISAPNFHANRPFVYFVMEKSTLTVLFSGIYSKPTV 56
             E              D +   +F A+ PF++ V E+ +  +LF G    P++
Sbjct: 339 DEEGTEAAAVSVASMTKDMLLMGDFVADHPFLFTVREEKSGVILFMGQVLDPSI 392


>At2g26390.1 68415.m03167 serpin, putative / serine protease
           inhibitor, putative similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311; contains Pfam profile
           PF00079: Serpin (serine protease inhibitor)
          Length = 389

 Score = 41.9 bits (94), Expect = 3e-04
 Identities = 30/113 (26%), Positives = 51/113 (45%), Gaps = 6/113 (5%)
 Frame = -1

Query: 379 VKIPRFQISTNLVLNKPLNDMGVYDIFQPDVASFQRVTK----ENIFVSAIVHKADIEVT 212
           ++IP+   S     ++ L DMG+   F       + V      + + VS+I+HKA IEV 
Sbjct: 275 LRIPKLNFSFEFKASEVLKDMGLTSPFTSKGNLTEMVDSPSNGDKLHVSSIIHKACIEVD 334

Query: 211 EXXXXXXXXXXXXXSDR--ISAPNFHANRPFVYFVMEKSTLTVLFSGIYSKPT 59
           E               +  +  P+F A+ PF++ V E ++  +LF G    P+
Sbjct: 335 EEGTEAAAVSVAIMMPQCLMRNPDFVADHPFLFTVREDNSGVILFIGQVLDPS 387


>At1g47710.1 68414.m05302 serpin, putative / serine protease
           inhibitor, putative similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311; contains Pfam profile
           PF00079: Serpin (serine protease inhibitor)
          Length = 391

 Score = 39.5 bits (88), Expect = 0.002
 Identities = 41/157 (26%), Positives = 64/157 (40%), Gaps = 9/157 (5%)
 Frame = -1

Query: 520 KYSMLFVIPYGNVKIQDMYKNFAEVSLKDIFKKLQDDVDKFGLEEIDVKIPRFQISTNLV 341
           ++SM F +P  N  + D+      VS       L + + +  ++  + KIP+F+ S    
Sbjct: 233 QFSMYFYLPDANNGLSDLLDKI--VSTPGF---LDNHIPRRQVKVREFKIPKFKFSFGFD 287

Query: 340 LNKPLNDMGVYDIFQPDVASFQRVTK----ENIFVSAIVHKADIEVTEXXXXXXXXXXXX 173
            +  L  +G+   F  +    + V      +N+ VS I HKA IEV E            
Sbjct: 288 ASNVLKGLGLTSPFSGEEGLTEMVESPEMGKNLCVSNIFHKACIEVNEEGTEAAAASAGV 347

Query: 172 XSDR-----ISAPNFHANRPFVYFVMEKSTLTVLFSG 77
              R         +F A+ PF+  V E  T  VLF G
Sbjct: 348 IKLRGLLMEEDEIDFVADHPFLLVVTENITGVVLFIG 384


>At1g64030.1 68414.m07252 serpin family protein / serine protease
           inhibitor family protein similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311, serpin [Triticum
           aestivum] GI:871551; contains Pfam profile PF00079:
           Serpin (serine protease inhibitor)
          Length = 385

 Score = 32.7 bits (71), Expect = 0.18
 Identities = 37/162 (22%), Positives = 67/162 (41%), Gaps = 5/162 (3%)
 Frame = -1

Query: 529 TDGKYSMLFVIPYGNVKIQDMYKNFAEVS--LKDIFKKLQDDVDKFGLEEIDVKIPRFQI 356
           T+ K+SM F +P     + D+ +  A     L       +D+++KF       +IP+F+I
Sbjct: 233 TNRKFSMYFYLPDKKDGLDDLLEKMASTPGFLDSHIPTYRDELEKF-------RIPKFKI 285

Query: 355 STNLVLNKPLNDMGVYDIFQPDVASFQRVTKENIFVSAIVHKADIEVTEXXXXXXXXXXX 176
                +   L+ +G+  +              +++  A V + D E  E           
Sbjct: 286 EFGFSVTSVLDRLGLRSM--------------SMYHKACV-EIDEEGAEAAAATADGDCG 330

Query: 175 XXSDRISAP---NFHANRPFVYFVMEKSTLTVLFSGIYSKPT 59
              D +  P   +F A+ PF++ + E+ T TVLF G    P+
Sbjct: 331 CSLDFVEPPKKIDFVADHPFLFLIREEKTGTVLFVGQIFDPS 372


>At1g62170.1 68414.m07013 serpin family protein / serine protease
           inhibitor family protein similar to phloem serpin-1
           GI:9937311 from [Cucurbita maxima]; contains Pfam
           profile PF00079: Serpin (serine protease inhibitor)
          Length = 433

 Score = 29.1 bits (62), Expect = 2.2
 Identities = 12/30 (40%), Positives = 20/30 (66%)
 Frame = -1

Query: 148 NFHANRPFVYFVMEKSTLTVLFSGIYSKPT 59
           +F A+ PF++ + E+ T TVLF+G    P+
Sbjct: 403 DFVADHPFLFLIREEQTGTVLFAGQIFDPS 432


>At1g10610.1 68414.m01202 basic helix-loop-helix (bHLH) family
           protein contains Pfam profile: PF00010 helix-loop-helix
           DNA-binding domain
          Length = 421

 Score = 27.9 bits (59), Expect = 5.1
 Identities = 15/35 (42%), Positives = 20/35 (57%)
 Frame = -1

Query: 439 KDIFKKLQDDVDKFGLEEIDVKIPRFQISTNLVLN 335
           +D FK+L + VD   LE IDV   R  ++   VLN
Sbjct: 382 QDGFKRLIEAVDLCELEIIDVNFTRLDLTVMTVLN 416


>At2g35580.1 68415.m04357 serpin family protein / serine protease
           inhibitor family protein similar to protein zx [Hordeum
           vulgare subsp. vulgare] GI:19071, serpin [Triticum
           aestivum] GI:1885350; contains Pfam profile PF00079:
           Serpin (serine protease inhibitor)
          Length = 374

 Score = 27.5 bits (58), Expect = 6.7
 Identities = 20/68 (29%), Positives = 29/68 (42%), Gaps = 4/68 (5%)
 Frame = -1

Query: 250 VSAIVHKADIEVTEXXXXXXXXXXXXXSDRISAP----NFHANRPFVYFVMEKSTLTVLF 83
           +S I+HK+ IEV E                   P    +F A+ PF++ V E  +  VLF
Sbjct: 305 LSMIMHKSCIEVDEVGSKAAAAAAFRGIGCRRPPPEKHDFVADHPFLFIVKEYRSGLVLF 364

Query: 82  SGIYSKPT 59
            G    P+
Sbjct: 365 LGQVMDPS 372


>At4g14310.1 68417.m02204 peroxisomal membrane protein-related
            contains weak similarity to Peroxisomal membrane protein
            2 (22 kDa peroxisomal membrane protein)
            (Swiss-Prot:P42925) [Mus musculus]
          Length = 1087

 Score = 27.1 bits (57), Expect = 8.9
 Identities = 12/36 (33%), Positives = 19/36 (52%), Gaps = 2/36 (5%)
 Frame = +3

Query: 267  VTRWKL-ATSGW-NISYTPISFKGLFRTRLVLIWNL 368
            +T WK     GW N  Y P+ F+ LF + +   W++
Sbjct: 1038 LTAWKFWPIVGWVNYQYVPLQFRVLFSSFVASCWSI 1073


>At2g14540.1 68415.m01628 serpin family protein / serine protease
           inhibitor family protein similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311; contains Pfam profile
           PF00079: Serpin (serine protease inhibitor)
          Length = 407

 Score = 27.1 bits (57), Expect = 8.9
 Identities = 11/30 (36%), Positives = 19/30 (63%)
 Frame = -1

Query: 148 NFHANRPFVYFVMEKSTLTVLFSGIYSKPT 59
           +F A+ PF++ + E  T T+LF+G    P+
Sbjct: 369 DFVADHPFLFLIREDKTGTLLFAGQIFDPS 398


>At1g64010.1 68414.m07250 serpin, putative / serine protease
           inhibitor, putative similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311; contains Pfam profile
           PF00079: Serpin (serine protease inhibitor)
          Length = 185

 Score = 27.1 bits (57), Expect = 8.9
 Identities = 12/30 (40%), Positives = 18/30 (60%)
 Frame = -1

Query: 148 NFHANRPFVYFVMEKSTLTVLFSGIYSKPT 59
           +F A+ PF++ + E  T TVLF G    P+
Sbjct: 153 DFVADHPFLFMIREDKTGTVLFVGQIFDPS 182


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,435,368
Number of Sequences: 28952
Number of extensions: 190404
Number of successful extensions: 486
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 476
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 481
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1112061928
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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