Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0005_J15
(418 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC354.02c |sec61||translocon alpha subunit Sec61|Schizosacchar... 144 5e-36
SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog |Schi... 79 2e-16
SPCC4G3.15c |||CCR4-Not complex subunit Not2 |Schizosaccharomyce... 27 0.88
SPAC17H9.13c |||glutamate 5-kinase |Schizosaccharomyces pombe|ch... 25 3.6
SPAC323.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 6.2
SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 25 6.2
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 24 8.2
SPCC825.02 |||glucosidase II Gtb1 |Schizosaccharomyces pombe|chr... 24 8.2
SPBC530.10c |anc1||adenine nucleotide carrier Anc1|Schizosacchar... 24 8.2
>SPBC354.02c |sec61||translocon alpha subunit
Sec61|Schizosaccharomyces pombe|chr 2|||Manual
Length = 479
Score = 144 bits (349), Expect = 5e-36
Identities = 73/137 (53%), Positives = 88/137 (64%)
Frame = +1
Query: 1 LVSNLYVISQMLAVKFSGNFLVNLLGVWADVGGGGPARAYPVGGLCYYLSPPESLSHIGQ 180
L SNL+ S++L +FS NFLV LGVW GL YYLSPP S
Sbjct: 306 LTSNLFFASRLLFNRFSSNFLVRFLGVWEQTA---------TSGLSYYLSPPASFQDALI 356
Query: 181 DPIHALLYIFFMLGSCAFFSKTWIDVSGSSAKDVAKQLKEQQMVMRGHRDNSMIHELNRY 360
DPIH L+Y+FF + +CA FSK WI+VSG+S +DVAKQLK QQ+VM GHR+ SM EL R
Sbjct: 357 DPIHTLVYVFFTMFACALFSKLWIEVSGASPRDVAKQLKSQQLVMAGHREGSMYKELKRI 416
Query: 361 IPTAAAFGGLCIGALSV 411
IPTAA G +GAL+V
Sbjct: 417 IPTAAWLSGAVVGALAV 433
>SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 475
Score = 79.4 bits (187), Expect = 2e-16
Identities = 41/139 (29%), Positives = 71/139 (51%)
Frame = +1
Query: 1 LVSNLYVISQMLAVKFSGNFLVNLLGVWADVGGGGPARAYPVGGLCYYLSPPESLSHIGQ 180
++S+L V + L + + LL ++ + + VGGL Y+L PP LS
Sbjct: 301 ILSHLLVFAYALYSLCPNSLITRLLVQYSPIDTFAEHKLQLVGGLVYFLYPPLGLSEALL 360
Query: 181 DPIHALLYIFFMLGSCAFFSKTWIDVSGSSAKDVAKQLKEQQMVMRGHRDNSMIHELNRY 360
P+H ++Y ++ +FS W++ + +DV KE Q+V+ G+R+ +M+ EL +
Sbjct: 361 HPVHTVIYTITLICITIYFSLLWMNATAGGPRDVLLFFKENQLVIAGYREATMLKELEKI 420
Query: 361 IPTAAAFGGLCIGALSVLA 417
IP AA + LSV+A
Sbjct: 421 IPIAAKLSAFFVSILSVIA 439
>SPCC4G3.15c |||CCR4-Not complex subunit Not2 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 176
Score = 27.5 bits (58), Expect = 0.88
Identities = 18/54 (33%), Positives = 28/54 (51%), Gaps = 3/54 (5%)
Frame = +1
Query: 67 NLLGVWADVGGGGPARAYPVGGL--CYY-LSPPESLSHIGQDPIHALLYIFFML 219
NL WA++ P + P+ L CY ++PP ++S I Q L YIF+ +
Sbjct: 44 NLFSPWAELNTKKPV-SQPMFKLPACYKNVNPPPAISKIFQFSDETLFYIFYTM 96
>SPAC17H9.13c |||glutamate 5-kinase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 402
Score = 25.4 bits (53), Expect = 3.6
Identities = 13/43 (30%), Positives = 23/43 (53%), Gaps = 1/43 (2%)
Frame = +1
Query: 235 FSKTWID-VSGSSAKDVAKQLKEQQMVMRGHRDNSMIHELNRY 360
+S T ID + G +K++A L + +RD ++H LN +
Sbjct: 350 YSSTEIDLIKGKRSKEIASILGYNETEYVAYRDYLVVHGLNSH 392
>SPAC323.03c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 575
Score = 24.6 bits (51), Expect = 6.2
Identities = 15/61 (24%), Positives = 30/61 (49%)
Frame = +1
Query: 196 LLYIFFMLGSCAFFSKTWIDVSGSSAKDVAKQLKEQQMVMRGHRDNSMIHELNRYIPTAA 375
LL +F+ L SC + + +K ++KQ+K Q G N ++ +L + ++A
Sbjct: 110 LLCVFYKLLSCDHIVNLQYPLKRAVSKALSKQIKTHQ--FSGFEANFLLQQLFASVDSSA 167
Query: 376 A 378
+
Sbjct: 168 S 168
>SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 3227
Score = 24.6 bits (51), Expect = 6.2
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = -2
Query: 321 SAHHHLLLFQLLRNILSGGSGDIDP 247
S H++L L + L +L G D+DP
Sbjct: 1521 SEHYYLELKESLPGVLQNGQTDLDP 1545
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 24.2 bits (50), Expect = 8.2
Identities = 10/26 (38%), Positives = 14/26 (53%)
Frame = +3
Query: 285 EAVERAADGDARTSRQLDDPRTEPLH 362
E VE+A D + +DD + PLH
Sbjct: 17 EYVEQAIQNDPNSLNAVDDDKRTPLH 42
>SPCC825.02 |||glucosidase II Gtb1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 506
Score = 24.2 bits (50), Expect = 8.2
Identities = 12/42 (28%), Positives = 18/42 (42%)
Frame = +3
Query: 285 EAVERAADGDARTSRQLDDPRTEPLHPDGGRLRWSLYRSSLG 410
EA +R D + L+ T+ +H W LYR+ G
Sbjct: 344 EAAQRDLDAAEENEKSLEKEHTKLMHELEYHHGWDLYRAIKG 385
>SPBC530.10c |anc1||adenine nucleotide carrier
Anc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 322
Score = 24.2 bits (50), Expect = 8.2
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = +1
Query: 31 MLAVKFSGNFLVNLLGVWADVGGGGPARAYPV 126
+L GNFL + L WA G G A +YP+
Sbjct: 223 VLVGPLEGNFLASFLLGWAVTTGSGVA-SYPL 253
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,445,701
Number of Sequences: 5004
Number of extensions: 24654
Number of successful extensions: 109
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 106
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 108
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 146319408
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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