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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0005_I21
         (556 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC191.09c |gst1||glutathione S-transferase Gst1|Schizosaccharo...    46   4e-06
SPCC965.07c |gst2||glutathione S-transferase Gst2|Schizosaccharo...    43   3e-05
SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyce...    42   8e-05
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz...    30   0.20 
SPAC6F12.05c |tnr3||thiamine diphosphokinase Tnr3 |Schizosacchar...    29   0.61 
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1...    29   0.61 
SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyc...    28   1.1  
SPBC1652.01 |||conserved fungal protein|Schizosaccharomyces pomb...    27   1.4  
SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase |Schizosa...    27   1.4  
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha...    26   3.2  
SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomy...    26   4.3  
SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase Ppk29|Schizos...    25   5.7  
SPAC2F3.01 ||SPAC323.09|mannosyltransferase complex subunit |Sch...    25   5.7  
SPAC24B11.12c |||P-type ATPase |Schizosaccharomyces pombe|chr 1|...    25   9.9  
SPAC589.09 |||sec14 cytosolic factor family|Schizosaccharomyces ...    25   9.9  
SPAC13C5.02 |dre4||DNA replication protein Dre4|Schizosaccharomy...    25   9.9  

>SPCC191.09c |gst1||glutathione S-transferase
           Gst1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 229

 Score = 46.0 bits (104), Expect = 4e-06
 Identities = 28/67 (41%), Positives = 35/67 (52%), Gaps = 4/67 (5%)
 Frame = +2

Query: 104 EHLKPEFLKLNPQHTVPTLVD---DGLSIWESRAIITYLVNKYGKGSSL-YPEDARARAL 271
           E   PE L LNP   VPTL+D   +  +IWES AI+ YL +KY     +  P D      
Sbjct: 40  EQKSPEHLALNPNGRVPTLIDHHNNDYTIWESDAILIYLADKYDTERKISLPRDHPEYYK 99

Query: 272 VDQRLYF 292
           V Q L+F
Sbjct: 100 VIQYLFF 106


>SPCC965.07c |gst2||glutathione S-transferase
           Gst2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 230

 Score = 42.7 bits (96), Expect = 3e-05
 Identities = 23/44 (52%), Positives = 27/44 (61%), Gaps = 3/44 (6%)
 Frame = +2

Query: 101 GEHLKPEFLKLNPQHTVPTLVD---DGLSIWESRAIITYLVNKY 223
           GE    E L LNP   VPTLVD   +  +IWES AI+ YL +KY
Sbjct: 39  GEQKCKEHLALNPNGRVPTLVDHKNNDYTIWESDAILIYLADKY 82


>SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 242

 Score = 41.5 bits (93), Expect = 8e-05
 Identities = 22/64 (34%), Positives = 33/64 (51%)
 Frame = +2

Query: 116 PEFLKLNPQHTVPTLVDDGLSIWESRAIITYLVNKYGKGSSLYPEDARARALVDQRLYFD 295
           P + KL+P    P +VDDG++  ES AI+ +LV KYG       ED       +  ++F 
Sbjct: 40  PAYTKLSPLGKSPIVVDDGVTYIESAAILEHLVRKYGPSFKPSEEDVAELEKYELWMHFS 99

Query: 296 IGTL 307
             +L
Sbjct: 100 EASL 103


>SPBC1306.01c ||SPBC409.22c|translation elongation factor
           G|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 770

 Score = 30.3 bits (65), Expect = 0.20
 Identities = 22/64 (34%), Positives = 35/64 (54%), Gaps = 3/64 (4%)
 Frame = -1

Query: 451 QIRAGCVLLTIEEFVQKLESVLDLGQLLXVGRRSGKYLRIEV---ISESLVEGTNVKVQA 281
           +I A  V + I +   KLE V+DL Q+  +  R  K  +IE+   + E+L+E    K  A
Sbjct: 226 KIPAAAVQIPIGQ-EDKLEGVVDLIQMRAIYNRGSKGEKIEISQQVPENLIELAKEKRSA 284

Query: 280 LIDE 269
           LI++
Sbjct: 285 LIEK 288


>SPAC6F12.05c |tnr3||thiamine diphosphokinase Tnr3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 569

 Score = 28.7 bits (61), Expect = 0.61
 Identities = 12/32 (37%), Positives = 22/32 (68%)
 Frame = +3

Query: 285 CTLTLVPSTRDSEITSIRKYLPERLPTKRSWP 380
           CT+  +P+T++  +   R ++P R PTK++WP
Sbjct: 139 CTM-YIPATKEHPL---RIWVPRRSPTKQTWP 166


>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 800

 Score = 28.7 bits (61), Expect = 0.61
 Identities = 34/118 (28%), Positives = 54/118 (45%), Gaps = 6/118 (5%)
 Frame = +3

Query: 195 PSSLTWSTSTVKAAASTQKTLERG----RSSISACTLTLVPSTRDSEITSIRKYLPERLP 362
           P + T STST    AST  T         +S S  T T +P T +S  T +   +P   P
Sbjct: 360 PPTSTSSTSTPPPPASTSSTGTSSSPLLSTSTSCTTSTSIPPTGNS-TTPVTPTVP---P 415

Query: 363 TKRSWPRSRTLSSFWTNSSMVKSTQPALI*P*LT--*ASSQACPASKRPISTSKNTLT 530
           T  S P   T ++  T++S+  ++ P    P  T    +S + P +  P++++  T T
Sbjct: 416 TSSSTP--LTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTSVPYTSTPVTSTPLTTT 471


>SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 721

 Score = 27.9 bits (59), Expect = 1.1
 Identities = 16/42 (38%), Positives = 23/42 (54%)
 Frame = +2

Query: 167 DGLSIWESRAIITYLVNKYGKGSSLYPEDARARALVDQRLYF 292
           + LSI ESR  I  +  ++G  S L+P D +  A  + RL F
Sbjct: 263 NSLSI-ESRLTIANMTTEWGALSGLFPTDEKLLAWYEDRLKF 303


>SPBC1652.01 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 386

 Score = 27.5 bits (58), Expect = 1.4
 Identities = 19/71 (26%), Positives = 35/71 (49%), Gaps = 5/71 (7%)
 Frame = +3

Query: 198 SSLTWSTSTVKAAASTQKTLERGRSSISACTLTLVPSTRDSEITSIRK-----YLPERLP 362
           SS+ W++++  +  +  K      SS+ +   + +PS+ ++  +S+ K     YLP  L 
Sbjct: 62  SSVKWASTSFDSTVTAHKEETPYSSSLGSHDSSSLPSSTNNRYSSVLKELCNTYLPSILS 121

Query: 363 TKRSWPRSRTL 395
           T  S P  R L
Sbjct: 122 TYGSLPIRRLL 132


>SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 440

 Score = 27.5 bits (58), Expect = 1.4
 Identities = 13/33 (39%), Positives = 19/33 (57%)
 Frame = +2

Query: 338 QVFAGAPADXEKLAKIEDALQLLDKFLDGQKYA 436
           Q+ +GAP   E+LAK  + L++ D   D   YA
Sbjct: 395 QMKSGAPCRSERLAKYNELLRIEDNLGDSAIYA 427


>SPAC1834.01 |sup45||translation release factor
           eRF1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 433

 Score = 26.2 bits (55), Expect = 3.2
 Identities = 8/26 (30%), Positives = 17/26 (65%)
 Frame = +2

Query: 164 DDGLSIWESRAIITYLVNKYGKGSSL 241
           +  + IW+ R ++  L+N +G G+S+
Sbjct: 6   EKAIEIWKIRRLVKQLINCHGNGTSM 31


>SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 648

 Score = 25.8 bits (54), Expect = 4.3
 Identities = 15/40 (37%), Positives = 22/40 (55%), Gaps = 3/40 (7%)
 Frame = +2

Query: 113 KPEFLKLNPQHTVPTLVDDGL---SIWESRAIITYLVNKY 223
           KP+FL  NPQ  V + V +GL   +  E    + YL+ +Y
Sbjct: 349 KPKFLDANPQSAVASRVLEGLIECAPAEKDLYLYYLIMRY 388


>SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase
           Ppk29|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 872

 Score = 25.4 bits (53), Expect = 5.7
 Identities = 9/18 (50%), Positives = 15/18 (83%)
 Frame = +3

Query: 486 PASKRPISTSKNTLTLTG 539
           P S+RP STS++++ +TG
Sbjct: 576 PVSRRPSSTSRSSVKITG 593


>SPAC2F3.01 ||SPAC323.09|mannosyltransferase complex subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 319

 Score = 25.4 bits (53), Expect = 5.7
 Identities = 12/29 (41%), Positives = 17/29 (58%)
 Frame = -1

Query: 304 GTNVKVQALIDERPRSSVFWVEAAAFTVL 218
           GT+ KV   ID   +S +F+   AAF +L
Sbjct: 263 GTDAKVFIWIDRNSKSVLFFAFLAAFAIL 291


>SPAC24B11.12c |||P-type ATPase |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1402

 Score = 24.6 bits (51), Expect = 9.9
 Identities = 13/29 (44%), Positives = 17/29 (58%)
 Frame = +3

Query: 468  ASSQACPASKRPISTSKNTLTLTGGMKQS 554
            +SS A P  K P+    N+LTLT  M +S
Sbjct: 1333 SSSLATP-KKEPLRLDTNSLTLTSSMPRS 1360


>SPAC589.09 |||sec14 cytosolic factor family|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 388

 Score = 24.6 bits (51), Expect = 9.9
 Identities = 10/27 (37%), Positives = 16/27 (59%)
 Frame = +3

Query: 303 PSTRDSEITSIRKYLPERLPTKRSWPR 383
           P T D ++ S   Y P +L  +++WPR
Sbjct: 34  PETVDEKV-SFESYSPLKLMIQKTWPR 59


>SPAC13C5.02 |dre4||DNA replication protein Dre4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 411

 Score = 24.6 bits (51), Expect = 9.9
 Identities = 12/32 (37%), Positives = 16/32 (50%)
 Frame = +3

Query: 339 KYLPERLPTKRSWPRSRTLSSFWTNSSMVKST 434
           +Y    + TK+   R  TLS FWT    + ST
Sbjct: 289 EYCKSVVSTKKITRRKNTLSDFWTLLHSLPST 320


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,961,458
Number of Sequences: 5004
Number of extensions: 34636
Number of successful extensions: 115
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 107
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 113
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 231978230
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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