BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0005_I18 (514 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g09990.1 68415.m01037 40S ribosomal protein S16 (RPS16A) Same... 215 1e-56 At5g18380.1 68418.m02162 40S ribosomal protein S16 (RPS16C) 215 1e-56 At3g04230.1 68416.m00447 40S ribosomal protein S16 (RPS16B) simi... 214 3e-56 At3g49080.1 68416.m05362 ribosomal protein S9 family protein con... 44 5e-05 At4g35250.1 68417.m05010 vestitone reductase-related low similar... 29 2.4 At3g24255.1 68416.m03045 expressed protein 29 2.4 At3g50430.1 68416.m05516 expressed protein 28 3.2 At5g40090.1 68418.m04863 disease resistance protein-related cont... 28 4.2 At5g20410.1 68418.m02427 1,2-diacylglycerol 3-beta-galactosyltra... 28 4.2 At2g41680.1 68415.m05149 thioredoxin reductase, putative / NADPH... 27 7.4 At5g43120.1 68418.m05264 tetratricopeptide repeat (TPR)-containi... 27 9.8 At1g43570.1 68414.m05001 hypothetical protein 27 9.8 At1g13000.1 68414.m01509 expressed protein contains Pfam profile... 27 9.8 >At2g09990.1 68415.m01037 40S ribosomal protein S16 (RPS16A) Same as GB:Q42340 Length = 146 Score = 215 bits (526), Expect = 1e-56 Identities = 95/143 (66%), Positives = 125/143 (87%) Frame = +3 Query: 15 ARREPIQAVQVFGRKKTATAVAYCKRGHGVLRVNGRPLDLVEPRLLQYKLQEPILLLGKE 194 A + ++VQ FGRKKTA AV +CKRG G++++NG P++L +P +L++K+ EPILLLGK Sbjct: 2 ATQPATESVQCFGRKKTAVAVTHCKRGSGLIKLNGCPIELFQPEILRFKIFEPILLLGKH 61 Query: 195 KFSGVDIRVTVKGGGHVAQVYAIRQAISKALIAFYQKYVDEASKKEIKDILVQYDRSLLV 374 +F+GV++R+ V GGGH +QVYAIRQ+I+KAL+A+YQKYVDE SKKEIKDILV+YDR+LLV Sbjct: 62 RFAGVNMRIRVNGGGHTSQVYAIRQSIAKALVAYYQKYVDEQSKKEIKDILVRYDRTLLV 121 Query: 375 ADPRRCEPKKFGGPGARARYQEN 443 ADPRRCEPKKFGG GAR+RYQ++ Sbjct: 122 ADPRRCEPKKFGGRGARSRYQKS 144 >At5g18380.1 68418.m02162 40S ribosomal protein S16 (RPS16C) Length = 146 Score = 215 bits (525), Expect = 1e-56 Identities = 94/143 (65%), Positives = 125/143 (87%) Frame = +3 Query: 15 ARREPIQAVQVFGRKKTATAVAYCKRGHGVLRVNGRPLDLVEPRLLQYKLQEPILLLGKE 194 A + ++VQ FGRKKTA AV +CKRG G++++NG P++L +P +L++K+ EP+LLLGK Sbjct: 2 ATQPATESVQCFGRKKTAVAVTHCKRGSGLIKLNGCPIELFQPEILRFKIFEPVLLLGKH 61 Query: 195 KFSGVDIRVTVKGGGHVAQVYAIRQAISKALIAFYQKYVDEASKKEIKDILVQYDRSLLV 374 +F+GV++R+ V GGGH +QVYAIRQ+I+KAL+A+YQKYVDE SKKEIKDILV+YDR+LLV Sbjct: 62 RFAGVNMRIRVNGGGHTSQVYAIRQSIAKALVAYYQKYVDEQSKKEIKDILVRYDRTLLV 121 Query: 375 ADPRRCEPKKFGGPGARARYQEN 443 ADPRRCEPKKFGG GAR+RYQ++ Sbjct: 122 ADPRRCEPKKFGGRGARSRYQKS 144 >At3g04230.1 68416.m00447 40S ribosomal protein S16 (RPS16B) similar to 40S ribosomal protein S16 GB:AAD22696 [Arabidopsis thaliana] Length = 146 Score = 214 bits (522), Expect = 3e-56 Identities = 92/137 (67%), Positives = 123/137 (89%) Frame = +3 Query: 33 QAVQVFGRKKTATAVAYCKRGHGVLRVNGRPLDLVEPRLLQYKLQEPILLLGKEKFSGVD 212 ++VQ FGRKKTATAV YCKRG G++++NG P++L +P +L++K+ EP+LLLGK +F+GVD Sbjct: 8 ESVQCFGRKKTATAVTYCKRGSGMIKLNGSPIELYQPEILRFKIFEPVLLLGKHRFAGVD 67 Query: 213 IRVTVKGGGHVAQVYAIRQAISKALIAFYQKYVDEASKKEIKDILVQYDRSLLVADPRRC 392 +R+ GGG+ ++VYAIRQ+I+KAL+A+YQKYVDE SKKEIKDIL++YDR+LLVADPRRC Sbjct: 68 MRIRATGGGNTSRVYAIRQSIAKALVAYYQKYVDEQSKKEIKDILMRYDRTLLVADPRRC 127 Query: 393 EPKKFGGPGARARYQEN 443 E KKFGGPGARAR+Q++ Sbjct: 128 ESKKFGGPGARARFQKS 144 >At3g49080.1 68416.m05362 ribosomal protein S9 family protein contains Pfam profile PF00380: ribosomal protein S9 Length = 430 Score = 44.4 bits (100), Expect = 5e-05 Identities = 28/79 (35%), Positives = 42/79 (53%) Frame = +3 Query: 51 GRKKTATAVAYCKRGHGVLRVNGRPLDLVEPRLLQYKLQEPILLLGKEKFSGVDIRVTVK 230 GR+K + A + + G G +VN + D+ P +L ++ L + DI+ TVK Sbjct: 310 GRRKCSIARVWIQPGEGKFQVNEKEFDVYFP-MLDHRAALLRPLAETKTLGRWDIKCTVK 368 Query: 231 GGGHVAQVYAIRQAISKAL 287 GGG QV AI+ IS+AL Sbjct: 369 GGGTTGQVGAIQLGISRAL 387 >At4g35250.1 68417.m05010 vestitone reductase-related low similarity to vestitone reductase [Medicago sativa subsp. sativa] GI:973249 Length = 395 Score = 28.7 bits (61), Expect = 2.4 Identities = 23/94 (24%), Positives = 36/94 (38%) Frame = -3 Query: 455 INGRIFLVSGTGAWTTEFLGLATTRVSDQQTSXXXXXXXXXXXLRCFVYIFLVESDKGFR 276 ING++ +G AWTT+ + R++ Q + R F +D R Sbjct: 273 INGKLLTFAGPRAWTTQEVITLCERLAGQDANVTTVPVSVLRVTRQLTRFFQWTNDVADR 332 Query: 275 DGLSDCINLCNMSTTFYSHSNIHTRELFLA*QKD 174 S+ ++ S T +S T L QKD Sbjct: 333 LAFSEVLS----SDTVFSAPMTETNSLLGVDQKD 362 >At3g24255.1 68416.m03045 expressed protein Length = 836 Score = 28.7 bits (61), Expect = 2.4 Identities = 18/42 (42%), Positives = 24/42 (57%) Frame = -1 Query: 388 RRGSATSKLLSY*TRMSLISFLDASSTYFW*KAIRALEMACL 263 R G T++ LS+ R+ LIS + S T FW A R L AC+ Sbjct: 142 RIGKWTARHLSFAGRLQLISSVIHSLTNFWMSAFR-LPSACI 182 >At3g50430.1 68416.m05516 expressed protein Length = 642 Score = 28.3 bits (60), Expect = 3.2 Identities = 14/29 (48%), Positives = 15/29 (51%) Frame = +1 Query: 364 VCWSLTLVVASPRNSVVQAPVPDTRKILP 450 VC S TL V P AP P RK+LP Sbjct: 553 VCDSWTLFVEFPFEGSTDAPSPKRRKVLP 581 >At5g40090.1 68418.m04863 disease resistance protein-related contains Pfam domain, PF00931: NB-ARC domain, a novel signalling motif found in plant resistance gene products Length = 385 Score = 27.9 bits (59), Expect = 4.2 Identities = 11/49 (22%), Positives = 26/49 (53%) Frame = +3 Query: 186 GKEKFSGVDIRVTVKGGGHVAQVYAIRQAISKALIAFYQKYVDEASKKE 332 G+E++ +++ K GGH+ +V+ + +A + ++ D +KE Sbjct: 310 GREEWVAALLKLKAKQGGHIMEVWKLMEATDDKGLEEWETAADIVERKE 358 >At5g20410.1 68418.m02427 1,2-diacylglycerol 3-beta-galactosyltransferase, putative / monogalactosyldiacylglycerol synthase, putative / MGDG synthase, putative identical to monogalactosyldiacylglycerol synthase [gi:3367638] from Arabidopsis thaliana, similar to MGDG synthase type A [gi:9884651] from Glycine max Length = 468 Score = 27.9 bits (59), Expect = 4.2 Identities = 17/45 (37%), Positives = 23/45 (51%) Frame = +3 Query: 174 ILLLGKEKFSGVDIRVTVKGGGHVAQVYAIRQAISKALIAFYQKY 308 ++ +G E+ V I ++ GGGH A AIR A I F KY Sbjct: 60 LIQIGAERTKNVLILMSDTGGGHRASAEAIRDAFK---IEFGDKY 101 >At2g41680.1 68415.m05149 thioredoxin reductase, putative / NADPH-dependent thioredoxin reductase, putative The last 2 exons encode thioredoxin. There is an EST match to exons 5-7, and the distance between exon 7 and exon 8 is only 90bp. It is unlikely this is two separate genes, but more likely a hybrid protein. Length = 529 Score = 27.1 bits (57), Expect = 7.4 Identities = 24/83 (28%), Positives = 38/83 (45%), Gaps = 2/83 (2%) Frame = +3 Query: 219 VTVKGGGHVAQVYAIRQAIS-KALIAFYQKYVDEASKK-EIKDILVQYDRSLLVADPRRC 392 VT G G +A + A R S L+ F+Q +EA K+ +D+ ++D +L Sbjct: 374 VTAAGSGCIAALSAERYLTSNNLLVEFHQPQTEEAKKEFTQRDVQEKFDITL-------- 425 Query: 393 EPKKFGGPGARARYQENPTVYII 461 K G R Y E+P V ++ Sbjct: 426 -TKHKGQYALRKLYHESPRVILV 447 >At5g43120.1 68418.m05264 tetratricopeptide repeat (TPR)-containing protein contains Pfam profile PF00515: TPR Domain Length = 588 Score = 26.6 bits (56), Expect = 9.8 Identities = 13/36 (36%), Positives = 21/36 (58%) Frame = +3 Query: 171 PILLLGKEKFSGVDIRVTVKGGGHVAQVYAIRQAIS 278 P+L L + K S V+ RV V+ GH+A +A++ Sbjct: 139 PLLELMRRKMSWVEQRVVVRALGHLASYETTFEAVA 174 >At1g43570.1 68414.m05001 hypothetical protein Length = 348 Score = 26.6 bits (56), Expect = 9.8 Identities = 15/36 (41%), Positives = 19/36 (52%) Frame = -1 Query: 391 QRRGSATSKLLSY*TRMSLISFLDASSTYFW*KAIR 284 +R S T + LSY R+ LI + S T FW A R Sbjct: 19 KRISSWTGRFLSYCGRLQLIKSVLMSITNFWSSAFR 54 >At1g13000.1 68414.m01509 expressed protein contains Pfam profile PF05212: Protein of unknown function (DUF707) Length = 401 Score = 26.6 bits (56), Expect = 9.8 Identities = 12/30 (40%), Positives = 16/30 (53%) Frame = +2 Query: 152 SVQTTGTYPFARQGKVLWCGY*SDCKRWWT 241 S+ + G+ A+ GK W G CKR WT Sbjct: 349 SLPSLGSQGEAQDGKAGWQGVRDRCKREWT 378 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,596,441 Number of Sequences: 28952 Number of extensions: 267985 Number of successful extensions: 749 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 723 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 748 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 927799552 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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