BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0005_I17
(558 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 29 0.46
SPBC19G7.10c |||topoisomerase associated protein |Schizosaccharo... 28 1.1
SPAC977.15 |||dienelactone hydrolase family|Schizosaccharomyces ... 26 3.3
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 26 3.3
SPCC830.05c |epl1||histone acetyltransferase complex subunit Epl... 26 4.3
SPAC17A5.14 |exo2||exonuclease II Exo2 |Schizosaccharomyces pomb... 25 5.7
SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizos... 25 5.7
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 25 7.5
SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce... 25 7.5
SPAC227.13c |isu1||mitochondrial iron-sulfur cluster assembly sc... 25 7.5
SPBC25B2.07c |mug164||microtubule-associated protein|Schizosacch... 25 7.5
SPCC1281.03c |||DUF1077 family protein|Schizosaccharomyces pombe... 25 10.0
SPAC6F6.13c |||DUF726 family protein|Schizosaccharomyces pombe|c... 25 10.0
SPCC1235.10c |sec6||exocyst complex subunit Sec6|Schizosaccharom... 25 10.0
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 29.1 bits (62), Expect = 0.46
Identities = 17/42 (40%), Positives = 20/42 (47%), Gaps = 2/42 (4%)
Frame = +2
Query: 335 RGGSRRHALG--PRDERTPQPPAATPTHGAEVAAQPARQLQP 454
R R ALG PR + TP P VAA A+Q+QP
Sbjct: 1240 RMNERLAALGIKPRQKGTPSPAPVNSATSTPVAAPTAQQIQP 1281
>SPBC19G7.10c |||topoisomerase associated protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 744
Score = 27.9 bits (59), Expect = 1.1
Identities = 19/55 (34%), Positives = 24/55 (43%), Gaps = 1/55 (1%)
Frame = +3
Query: 363 VHVTNAPRNLLQLPPHTEQRSPLSLLGNFNHMFDAK-TRHEADKLYSRRHQNEQA 524
V+ PR LL + TE S S L NF+ K H +K+Y EQA
Sbjct: 419 VNTVRTPRQLLNVKRPTEPASSNSSLNNFSGFSTKKDVLHAIEKVYDLLLDFEQA 473
>SPAC977.15 |||dienelactone hydrolase family|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 247
Score = 26.2 bits (55), Expect = 3.3
Identities = 9/19 (47%), Positives = 13/19 (68%)
Frame = -2
Query: 179 PSPLDPIEAAFTNCSQCFV 123
PS LDP++A +C CF+
Sbjct: 160 PSLLDPVDAKHVHCPVCFL 178
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 26.2 bits (55), Expect = 3.3
Identities = 17/60 (28%), Positives = 25/60 (41%)
Frame = +2
Query: 308 RASPRHSVLRGGSRRHALGPRDERTPQPPAATPTHGAEVAAQPARQLQPHVRRENQARSR 487
++ P + GGS A GPR +P A P ++PA P V Q ++R
Sbjct: 967 KSKPFPRLATGGSTAAARGPRPVVQNKPAATKPVSMPAAKSKPAPMANP-VSTAQQTQNR 1025
>SPCC830.05c |epl1||histone acetyltransferase complex subunit Epl1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 557
Score = 25.8 bits (54), Expect = 4.3
Identities = 10/24 (41%), Positives = 12/24 (50%)
Frame = +2
Query: 380 TPQPPAATPTHGAEVAAQPARQLQ 451
TP PP T H A QP R ++
Sbjct: 331 TPAPPVKTSPHPASYRPQPTRNVE 354
>SPAC17A5.14 |exo2||exonuclease II Exo2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1328
Score = 25.4 bits (53), Expect = 5.7
Identities = 14/41 (34%), Positives = 21/41 (51%)
Frame = -2
Query: 224 KNIFLTTQLK*MLKVPSPLDPIEAAFTNCSQCFVQRICKEL 102
KN FL Q+K L P A ++ Q F++R+C +L
Sbjct: 389 KNYFL--QIKKFLSSREPFIDFSANISSVDQRFLRRLCNDL 427
>SPBC17D11.05 |tif32||translation initiation factor
eIF3a|Schizosaccharomyces pombe|chr 2|||Manual
Length = 932
Score = 25.4 bits (53), Expect = 5.7
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = +2
Query: 308 RASPRHSVLRGGSRRHALGPRDERTPQPPAATP 406
R SPR+S RGG R ++ PR+ P A P
Sbjct: 893 RDSPRYS--RGGYSRGSVPPRETLAPSKGAYVP 923
>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
Sen1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1687
Score = 25.0 bits (52), Expect = 7.5
Identities = 10/28 (35%), Positives = 15/28 (53%), Gaps = 1/28 (3%)
Frame = -1
Query: 285 SGMLCLVELLRSFRPDVEH-PQKYFLDN 205
S +CL+ + PD+ H P K F D+
Sbjct: 1435 SNQMCLLSIQYRMHPDISHFPSKKFYDS 1462
>SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1242
Score = 25.0 bits (52), Expect = 7.5
Identities = 11/37 (29%), Positives = 19/37 (51%)
Frame = +1
Query: 157 SIGSRGLGTLSIYLSWVVKKIFLRMFYIRSKTAEQLY 267
+I +RG L + +WV+ LRM Y + E+ +
Sbjct: 1083 TIFNRGTLALDAFQNWVIGLFMLRMIYFTVTSNEERF 1119
>SPAC227.13c |isu1||mitochondrial iron-sulfur cluster assembly
scaffold protein Isu1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 192
Score = 25.0 bits (52), Expect = 7.5
Identities = 12/43 (27%), Positives = 20/43 (46%)
Frame = +3
Query: 153 GLYRVKRTGDLKHLLELGCQENIFEDVLHQVENCGAALQGRAY 281
GL GD+ L ++ + EDV + CG+A+ +Y
Sbjct: 81 GLVGAPACGDVMRLAIRVNKDGVIEDVKFKTFGCGSAIASSSY 123
>SPBC25B2.07c |mug164||microtubule-associated
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 501
Score = 25.0 bits (52), Expect = 7.5
Identities = 16/57 (28%), Positives = 27/57 (47%)
Frame = +1
Query: 238 IRSKTAEQLYKAEHTRCRNPEAVTCVTSPLCASRRIAPSRSGST*RTHPATSCSYPH 408
+R+K A + + R P ++ P R +APSRS +T P++S + H
Sbjct: 382 VRTKAANIVRPSSSINRRPPSSIN--QRPPSNLRILAPSRSRATIHERPSSSILHRH 436
>SPCC1281.03c |||DUF1077 family protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 193
Score = 24.6 bits (51), Expect = 10.0
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +1
Query: 181 TLSIYLSWVVKKIFLRMFYIRSKT 252
T S +L+W V K+F+ Y SKT
Sbjct: 168 TTSDWLAWEVSKVFMDRSYGPSKT 191
>SPAC6F6.13c |||DUF726 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 778
Score = 24.6 bits (51), Expect = 10.0
Identities = 10/43 (23%), Positives = 22/43 (51%)
Frame = +1
Query: 238 IRSKTAEQLYKAEHTRCRNPEAVTCVTSPLCASRRIAPSRSGS 366
+R + ++ LY+ E C+N + S + R ++P +S +
Sbjct: 709 LRERQSQLLYEIEAEECQNKQKELIEKSLMQKGRSLSPKKSNA 751
>SPCC1235.10c |sec6||exocyst complex subunit
Sec6|Schizosaccharomyces pombe|chr 3|||Manual
Length = 730
Score = 24.6 bits (51), Expect = 10.0
Identities = 12/41 (29%), Positives = 21/41 (51%)
Frame = +3
Query: 375 NAPRNLLQLPPHTEQRSPLSLLGNFNHMFDAKTRHEADKLY 497
NAP ++ ++ P E L+ + +F K R +DKL+
Sbjct: 343 NAPFSVDEVEPGLEDGKDGILVREYTRLFTQKIREWSDKLF 383
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,299,162
Number of Sequences: 5004
Number of extensions: 47129
Number of successful extensions: 160
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 158
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 160
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 233995432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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