Samia BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0005_H08
         (650 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein co...    64   6e-11
At3g55260.1 68416.m06137 glycosyl hydrolase family 20 protein si...    53   1e-07
At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein si...    53   1e-07
At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-con...    28   4.7  
At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-con...    28   4.7  
At2g25730.1 68415.m03084 expressed protein                             28   6.2  

>At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein
           contains Pfam PF00728: Glycosyl hydrolase family 20,
           catalytic domain; contains Pfam PF02838: Glycosyl
           hydrolase family 20, domain 2; similar to
           Beta-hexosaminidase beta chain precursor (EC 3.2.1.52)
           (N-acetyl-beta- glucosaminidase)
           (Beta-N-acetylhexosaminidase) (Hexosaminidase B)
           (Swiss-Prot:P07686) [Homo sapiens]
          Length = 535

 Score = 64.5 bits (150), Expect = 6e-11
 Identities = 38/98 (38%), Positives = 56/98 (57%), Gaps = 7/98 (7%)
 Frame = +3

Query: 372 VQELHVELTAPCEKLPYFGMDESYNLXXXX-----XXXXXXXXIWGVIRGLETWSQL--F 530
           +Q LHV +++  ++L Y G DESY L                 ++G + GL+T+SQL  F
Sbjct: 102 LQGLHVIISSSTDELEY-GADESYKLVVPSPEKPSYAQLEAKSVYGALHGLQTFSQLCHF 160

Query: 531 YLTNDFRELRINSTDIYDYPRYAHRGLLLDTSRHYLSL 644
            L     E+ +   +I D PR+++RGLL+DTSRHYL L
Sbjct: 161 NLKKKVIEILMTPWNIIDQPRFSYRGLLIDTSRHYLPL 198


>At3g55260.1 68416.m06137 glycosyl hydrolase family 20 protein
           similar to beta-hexosaminidase A SP:P13723 from
           [Dictyostelium discoideum]
          Length = 541

 Score = 53.2 bits (122), Expect = 1e-07
 Identities = 34/101 (33%), Positives = 51/101 (50%), Gaps = 10/101 (9%)
 Frame = +3

Query: 372 VQELHVELTAPCEKLPYFGMDESYNLXXXX--------XXXXXXXXIWGVIRGLETWSQL 527
           +  L + + +  E+L   G+DESY L                    ++G +RGLET+SQL
Sbjct: 102 ITSLKIVVHSDSEELQ-LGVDESYTLMVSKKNEQSIVGAATIEANTVYGALRGLETFSQL 160

Query: 528 FYLTNDFRELRINSTDIY--DYPRYAHRGLLLDTSRHYLSL 644
                  + ++I     Y  D PR+ +RGLL+DTSRHYL +
Sbjct: 161 CAFDYITKSVQIYKAPWYIQDKPRFGYRGLLIDTSRHYLPI 201


>At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein
           similar to beta-hexosaminidase precursor SP:P43077 from
           [Candida albicans]
          Length = 580

 Score = 53.2 bits (122), Expect = 1e-07
 Identities = 30/75 (40%), Positives = 41/75 (54%), Gaps = 3/75 (4%)
 Frame = +3

Query: 420 YFGMDESYNLXX---XXXXXXXXXXIWGVIRGLETWSQLFYLTNDFRELRINSTDIYDYP 590
           + G+DESY L                WG +RGLET+SQ+ + T+    L +    I D P
Sbjct: 111 HHGVDESYKLSIPIGSFSAHLLAHSAWGAMRGLETFSQMIWGTSPDLCLPVGIY-IQDSP 169

Query: 591 RYAHRGLLLDTSRHY 635
            + HRG+LLDTSR+Y
Sbjct: 170 LFGHRGVLLDTSRNY 184


>At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon
           domain-containing protein low similarity to SP|Q13144
           Translation initiation factor eIF-2B epsilon subunit
           (eIF-2B GDP-GTP exchange factor) {Homo sapiens};
           contains Pfam profile PF02020:
           eIF4-gamma/eIF5/eIF2-epsilon
          Length = 411

 Score = 28.3 bits (60), Expect = 4.7
 Identities = 10/25 (40%), Positives = 18/25 (72%)
 Frame = +3

Query: 228 FLTKALERYQFLVRDLHRITRRFVK 302
           ++ K L R  FL+++L  +TRRF++
Sbjct: 109 YIQKILRRKPFLIKNLENVTRRFLQ 133


>At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon
           domain-containing protein low similarity to SP|P47823
           Translation initiation factor eIF-2B epsilon subunit
           (eIF-2B GDP-GTP exchange factor) {Oryctolagus
           cuniculus}; contains Pfam profile PF02020:
           eIF4-gamma/eIF5/eIF2-epsilon
          Length = 411

 Score = 28.3 bits (60), Expect = 4.7
 Identities = 10/25 (40%), Positives = 18/25 (72%)
 Frame = +3

Query: 228 FLTKALERYQFLVRDLHRITRRFVK 302
           ++ K L R  FL+++L  +TRRF++
Sbjct: 109 YIQKILRRKAFLIKNLENVTRRFLQ 133


>At2g25730.1 68415.m03084 expressed protein 
          Length = 2464

 Score = 27.9 bits (59), Expect = 6.2
 Identities = 15/52 (28%), Positives = 26/52 (50%)
 Frame = +3

Query: 237  KALERYQFLVRDLHRITRRFVKRSLGSQYRSLDDEAANDERFQGTVQELHVE 392
            +AL     ++ D HR  R+F+   L +  R+L DE    +  +G   ++ VE
Sbjct: 915  RALAMLHQMIEDAHRGKRQFLSGKLHNLARALADEKPEVDVLKGDGSDMAVE 966


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,093,633
Number of Sequences: 28952
Number of extensions: 331240
Number of successful extensions: 755
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 736
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 752
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1354097952
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)