Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0005_H03
(694 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g40770.1 68415.m05030 SNF2 domain-containing protein / helica... 30 1.3
At4g25940.1 68417.m03731 epsin N-terminal homology (ENTH) domain... 30 1.7
At5g57200.1 68418.m07145 epsin N-terminal homology (ENTH) domain... 29 2.2
At5g22540.1 68418.m02630 expressed protein contains Pfam profile... 29 2.2
At4g11310.1 68417.m01827 cysteine proteinase, putative contains ... 29 2.2
At2g45870.1 68415.m05705 expressed protein contains Pfam profile... 29 2.2
At3g52970.1 68416.m05839 cytochrome P450 family protein cytochro... 28 5.1
At5g15510.1 68418.m01816 expressed protein 27 8.9
At1g64290.1 68414.m07285 F-box protein-related contains TIGRFAM ... 27 8.9
At1g13260.1 68414.m01539 DNA-binding protein RAV1 (RAV1) identic... 27 8.9
>At2g40770.1 68415.m05030 SNF2 domain-containing protein / helicase
domain-containing protein / zinc finger (C3HC4 type RING
finger) family protein low similarity to SP|P36607 DNA
repair protein rad8 {Schizosaccharomyces pombe};
contains Pfam profiles PF00271: Helicase conserved
C-terminal domain, PF00176: SNF2 family N-terminal
domain, PF00628: PHD-finger, PF00097: Zinc finger, C3HC4
type (RING finger)
Length = 1648
Score = 30.3 bits (65), Expect = 1.3
Identities = 14/48 (29%), Positives = 24/48 (50%)
Frame = +3
Query: 147 ARKLISTMKRQLTLSETIGKRTPICMKKKLQRIINDLMKLSLAMCSVQ 290
AR++I T+KR + P+ + +++N L+KL A C Q
Sbjct: 719 AREVIETLKRDILKRGHTSSDNPLVTHAEAAKLLNSLLKLRQACCHPQ 766
>At4g25940.1 68417.m03731 epsin N-terminal homology (ENTH)
domain-containing protein contains Pfam PF01417: ENTH
domain. ENTH (Epsin N-terminal homology) domain; similar
to Chain B, Crystal Structure Of N-Terminal Domain Of
Drosophila Ap180 (GP:13399617) [Drosophila
melanogaster]; supporting cDNA
gi|20465326|gb|AY096427.1|
Length = 601
Score = 29.9 bits (64), Expect = 1.7
Identities = 35/132 (26%), Positives = 59/132 (44%), Gaps = 14/132 (10%)
Frame = +2
Query: 200 WQENADLYEEEVTKDYQ-RSYEIVARHV---LGAAPKPFD---KHTFMPSALDFYQTALR 358
W L+ EE + Y+ Y+I A + GA+ K D T+ L + +
Sbjct: 139 WIRTYALFLEERLECYRVLKYDIEAERLPKGSGASSKNVDFNASQTYRTRMLSDEELLEQ 198
Query: 359 DPAFYQLYXRIVGYI---NAFKHYLKPYPQE---KLHF-VGVKINDVVVEKLVTFFDYSQ 517
PA QL R++G +A+ +YL Y K F + IND ++ + FF+ S+
Sbjct: 199 LPALQQLLYRLIGCQPEGSAYSNYLIQYALALVLKESFKIYCAINDGIINLVDMFFEMSR 258
Query: 518 FDATNSVFLTKK 553
DA ++ + K+
Sbjct: 259 HDAVKALNIYKR 270
>At5g57200.1 68418.m07145 epsin N-terminal homology (ENTH)
domain-containing protein / clathrin assembly
protein-related low similarity to clathrin assembly
protein AP180 [Xenopus laevis] GI:6492344; contains Pfam
profile PF01417: ENTH domain
Length = 591
Score = 29.5 bits (63), Expect = 2.2
Identities = 35/127 (27%), Positives = 56/127 (44%), Gaps = 9/127 (7%)
Frame = +2
Query: 200 WQENADLYEEEVTKDYQ-RSYEIVARHVLGAAPKPFDKH-TFMPSALDFYQTALRDPAFY 373
W L+ EE + Y+ Y+I A + A+ H T M S D + PA
Sbjct: 139 WVRTYALFLEERLECYRVLKYDIEAERLPKASGAASKTHRTRMLSGEDLLEQL---PALQ 195
Query: 374 QLYXRIVGYIN---AFKHYLKPYPQE---KLHF-VGVKINDVVVEKLVTFFDYSQFDATN 532
QL R++G A+ +YL Y K F + IND ++ + FF+ S+ DA
Sbjct: 196 QLLYRLIGCQPEGAAYSNYLIQYALALVLKESFKIYCAINDGIINLVDMFFEMSRHDAVK 255
Query: 533 SVFLTKK 553
++ + K+
Sbjct: 256 ALNIYKR 262
>At5g22540.1 68418.m02630 expressed protein contains Pfam profile
PF03140: Plant protein of unknown function
Length = 440
Score = 29.5 bits (63), Expect = 2.2
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = +2
Query: 71 EAIRFLDIFEKTFVQSLQKGKFESYGKKIDFHDEKAINFV 190
EA LD+ KTFV + + + + K F+D + + FV
Sbjct: 217 EAKHLLDLIRKTFVPVPSQRRIKDHSSKSSFNDHEYLGFV 256
>At4g11310.1 68417.m01827 cysteine proteinase, putative contains
similarity to cysteine proteinase RD21A (thiol protease)
GI:435619, SP:P43297 from [Arabidopsis thaliana]
Length = 364
Score = 29.5 bits (63), Expect = 2.2
Identities = 32/109 (29%), Positives = 47/109 (43%), Gaps = 1/109 (0%)
Frame = +2
Query: 38 DNYNLHSVKNYEAIRFLDIFEKTFVQSLQK-GKFESYGKKIDFHDEKAINFVGNYWQENA 214
DN LHSV + EA IFE V+ + G +++ ++ + F+ N EN
Sbjct: 33 DNNRLHSVFDAEASL---IFESWMVKHGKVYGSVAEKERRLTIFEDN-LRFINNRNAENL 88
Query: 215 DLYEEEVTKDYQRSYEIVARHVLGAAPKPFDKHTFMPSALDFYQTALRD 361
Y +T S GA P+P H FM S+ D Y+T+ D
Sbjct: 89 S-YRLGLTGFADLSLHEYKEVCHGADPRPPRNHVFMTSS-DRYKTSADD 135
>At2g45870.1 68415.m05705 expressed protein contains Pfam profile
PF05249: Uncharacterised protein family (UPF0187)
Length = 410
Score = 29.5 bits (63), Expect = 2.2
Identities = 24/77 (31%), Positives = 40/77 (51%)
Frame = +3
Query: 126 KANSNRMARKLISTMKRQLTLSETIGKRTPICMKKKLQRIINDLMKLSLAMCSVQHLNHS 305
K++ + ++ KLIS +K S+ I +R + K+ L N + S S++HL H
Sbjct: 49 KSDDSPLSEKLISLLKAVPNWSDGIKERR-MQQKRSLYTHENWVRHRS----SLRHLRHV 103
Query: 306 TSTPSCPVRLTFTKPHF 356
+S+PS V L+ P F
Sbjct: 104 SSSPSSRVILSLIPPVF 120
>At3g52970.1 68416.m05839 cytochrome P450 family protein cytochrome
P450 76A2, eggplant, PIR:S38534
Length = 516
Score = 28.3 bits (60), Expect = 5.1
Identities = 14/37 (37%), Positives = 20/37 (54%), Gaps = 4/37 (10%)
Frame = +2
Query: 149 KKIDFHDEKAINFVGNYWQENADLYE----EEVTKDY 247
+K FH EKA G + +E ++ E +E TKDY
Sbjct: 242 RKTQFHVEKAFEIAGEFIRERTEVREREKSDEKTKDY 278
>At5g15510.1 68418.m01816 expressed protein
Length = 497
Score = 27.5 bits (58), Expect = 8.9
Identities = 21/77 (27%), Positives = 36/77 (46%), Gaps = 3/77 (3%)
Frame = +2
Query: 152 KIDFHDEKAINFVGNYWQENA---DLYEEEVTKDYQRSYEIVARHVLGAAPKPFDKHTFM 322
+ D+ + ++F+ Y E L EEE + ++ E+V + A P P+ F+
Sbjct: 397 EFDYQVAEKMSFIEQYKMERERQQKLAEEEEIRRLRK--ELVPK----AQPMPYFDRPFI 450
Query: 323 PSALDFYQTALRDPAFY 373
P + TA RDP F+
Sbjct: 451 PRRSSKHPTAPRDPKFH 467
>At1g64290.1 68414.m07285 F-box protein-related contains TIGRFAM
TIGR01640 : F-box protein interaction domain;
Length = 364
Score = 27.5 bits (58), Expect = 8.9
Identities = 16/50 (32%), Positives = 26/50 (52%), Gaps = 3/50 (6%)
Frame = -1
Query: 604 ESWLTNLEVVWVTSLNLFFGQEYTVSG--IKLAIVKECD*FLNDNI-IDF 464
ESW NL ++ ++ +F Q Y V G ++ + V E N+ + IDF
Sbjct: 39 ESWFVNLNLLRTNRISGYFIQHYIVKGHELRTSFVHERSDLQNNGVSIDF 88
>At1g13260.1 68414.m01539 DNA-binding protein RAV1 (RAV1) identical
to SP|Q9ZWM9 DNA-binding protein RAV1 {Arabidopsis
thaliana}, RAV1 GI:3868857 from [Arabidopsis thaliana]
Length = 344
Score = 27.5 bits (58), Expect = 8.9
Identities = 21/75 (28%), Positives = 35/75 (46%)
Frame = +2
Query: 326 SALDFYQTALRDPAFYQLYXRIVGYINAFKHYLKPYPQEKLHFVGVKINDVVVEKLVTFF 505
SA ++ A+ +L ++ +A KH+ P P + GV +N V V F
Sbjct: 183 SAEALFEKAVTPSDVGKLNRLVIPKHHAEKHF--PLPSSNVSVKGVLLNFEDVNGKVWRF 240
Query: 506 DYSQFDATNSVFLTK 550
YS ++++ S LTK
Sbjct: 241 RYSYWNSSQSYVLTK 255
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,089,110
Number of Sequences: 28952
Number of extensions: 285284
Number of successful extensions: 805
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 791
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 805
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1477286152
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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