BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0005_G19
(419 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_6783| Best HMM Match : Porphobil_deamC (HMM E-Value=5.8) 140 4e-34
SB_23832| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 0.89
SB_7305| Best HMM Match : Extensin_2 (HMM E-Value=0.043) 29 2.1
SB_6508| Best HMM Match : AMP-binding (HMM E-Value=2.8026e-45) 29 2.1
SB_4910| Best HMM Match : HECT (HMM E-Value=5.8e-33) 29 2.1
SB_3165| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 2.1
SB_41190| Best HMM Match : Extensin_2 (HMM E-Value=0.0029) 29 2.1
SB_14110| Best HMM Match : DUF858 (HMM E-Value=2) 28 3.6
SB_29100| Best HMM Match : TPMT (HMM E-Value=5.4e-33) 27 4.8
SB_3686| Best HMM Match : Myotub-related (HMM E-Value=2.8e-08) 27 6.3
SB_1712| Best HMM Match : Peptidase_C14 (HMM E-Value=1.6e-21) 27 6.3
SB_41291| Best HMM Match : Piwi (HMM E-Value=0) 27 6.3
SB_52816| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 8.3
SB_39182| Best HMM Match : Cellulase (HMM E-Value=0.00018) 27 8.3
SB_23673| Best HMM Match : DUF855 (HMM E-Value=0.52) 27 8.3
>SB_6783| Best HMM Match : Porphobil_deamC (HMM E-Value=5.8)
Length = 382
Score = 140 bits (339), Expect = 4e-34
Identities = 59/115 (51%), Positives = 84/115 (73%)
Frame = +3
Query: 30 IMSADQVFHSRSEGIPSEGVKDQYADGKAARAWNKFIGDSNERTQNYKDFLIGLLKKHGC 209
+MS D V+ +RS G+P+ G+ DQYADGKAA+ W +IG +RT++Y++F LL++
Sbjct: 148 VMSMDGVYRTRSLGVPATGIPDQYADGKAAKVWQHYIGGHKKRTESYREFFCNLLRERNI 207
Query: 210 KKVLDGACGTGIDSMMLVDEGFNLVSVDASDKMLKHALKARWEKRKNPKYDEWVI 374
VLD +CGTG+DS+ML++ GF + SVDASDKMLK AL+ RW +RK +D+W I
Sbjct: 208 HNVLDVSCGTGVDSIMLLENGFCVTSVDASDKMLKDALRIRWNRRKEEPFDKWGI 262
>SB_23832| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 706
Score = 29.9 bits (64), Expect = 0.89
Identities = 30/112 (26%), Positives = 49/112 (43%)
Frame = +3
Query: 63 SEGIPSEGVKDQYADGKAARAWNKFIGDSNERTQNYKDFLIGLLKKHGCKKVLDGACGTG 242
S I EG+ + A R W+ + + +R YK + + +GC VLD G+G
Sbjct: 117 SSTIAREGL-ESVASALVER-WHFRMLNDRQRNLAYKKAISNAVS-NGCDIVLDIGSGSG 173
Query: 243 IDSMMLVDEGFNLVSVDASDKMLKHALKARWEKRKNPKYDEWVIEEANWETL 398
I SM V G V + +++K + +++N D+ IE E L
Sbjct: 174 ILSMFAVQAGAKKVYACFNVRIVK-VIGGEQLEQENTHLDQEAIEPYTTECL 224
>SB_7305| Best HMM Match : Extensin_2 (HMM E-Value=0.043)
Length = 908
Score = 28.7 bits (61), Expect = 2.1
Identities = 24/72 (33%), Positives = 32/72 (44%), Gaps = 9/72 (12%)
Frame = -2
Query: 313 FNILSEASTETRLKPSSTSIMESIPVPQAPSSTFL-----HPCFF--KSPMRKSL*FCVL 155
F L +T PS+T + +IP PQ P T L HP F P + SL F +
Sbjct: 684 FRTLENLATLRYFGPSNTLLHFAIPHPQIPHYTSLFRTLKHPTTFPYSGPSKTSLHFAIS 743
Query: 154 SLLSP--MNLFH 125
+P +LFH
Sbjct: 744 DPRTPYYTSLFH 755
Score = 27.1 bits (57), Expect = 6.3
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = -2
Query: 322 SACFNILSEASTETRLKPSSTSIMESIPVPQAPSSTFL 209
++ F+ L +T PS+TS+ +IP PQ P T L
Sbjct: 856 TSLFHTLEHLTTLRYSTPSNTSLHFAIPDPQTPHYTSL 893
>SB_6508| Best HMM Match : AMP-binding (HMM E-Value=2.8026e-45)
Length = 1038
Score = 28.7 bits (61), Expect = 2.1
Identities = 16/42 (38%), Positives = 23/42 (54%)
Frame = -2
Query: 385 LASSITHSSYFGFFLFSHLALSACFNILSEASTETRLKPSST 260
LA S T+ + HLA S+ + L+++ST RL SST
Sbjct: 808 LADSSTYEHLADSCTYEHLADSSTYEHLADSSTYARLADSST 849
Score = 26.6 bits (56), Expect = 8.3
Identities = 20/63 (31%), Positives = 33/63 (52%), Gaps = 3/63 (4%)
Frame = -2
Query: 385 LASSITHSSYFGFFLFSHLALSACFNILSEASTETRLKPSST--SIMESIPVPQ-APSST 215
LA S T++ + HLA ++ + L+++ST L+ SST + +S A SST
Sbjct: 835 LADSSTYARLADSSTYEHLADNSTYEHLADSSTYEHLEDSSTYEHLADSSTYEHLADSST 894
Query: 214 FLH 206
+ H
Sbjct: 895 YEH 897
Score = 26.6 bits (56), Expect = 8.3
Identities = 21/65 (32%), Positives = 32/65 (49%), Gaps = 3/65 (4%)
Frame = -2
Query: 391 SQLASSITHSSYFGFFLFSHLALSACFNILSEASTETRLKPSST--SIMESIPVPQ-APS 221
++LA S T+ + HLA S+ + L ++ST L SST + +S A S
Sbjct: 842 ARLADSSTYEHLADNSTYEHLADSSTYEHLEDSSTYEHLADSSTYEHLADSSTYEHLADS 901
Query: 220 STFLH 206
ST+ H
Sbjct: 902 STYEH 906
>SB_4910| Best HMM Match : HECT (HMM E-Value=5.8e-33)
Length = 958
Score = 28.7 bits (61), Expect = 2.1
Identities = 24/72 (33%), Positives = 32/72 (44%), Gaps = 9/72 (12%)
Frame = -2
Query: 313 FNILSEASTETRLKPSSTSIMESIPVPQAPSSTFL-----HPCFF--KSPMRKSL*FCVL 155
F L +T PS+T + +IP PQ P T L HP F P + SL F +
Sbjct: 633 FRTLENLATLRYFGPSNTLLHFAIPHPQIPHYTSLFRTLKHPTTFPYSGPSKTSLHFAIS 692
Query: 154 SLLSP--MNLFH 125
+P +LFH
Sbjct: 693 DPRTPYYTSLFH 704
>SB_3165| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1183
Score = 28.7 bits (61), Expect = 2.1
Identities = 21/82 (25%), Positives = 36/82 (43%)
Frame = -2
Query: 397 NVSQLASSITHSSYFGFFLFSHLALSACFNILSEASTETRLKPSSTSIMESIPVPQAPSS 218
+++ AS + +S+ F F+ F I AST + P+S + S+ P +P+S
Sbjct: 655 SLTSFASFTSFTSFTSFASFTSFTSFTSFAIALLASTASPASPASLASPASLASPASPAS 714
Query: 217 TFLHPCFFKSPMRKSL*FCVLS 152
P SP + F + S
Sbjct: 715 -LASPASLASPASTTTAFYLAS 735
>SB_41190| Best HMM Match : Extensin_2 (HMM E-Value=0.0029)
Length = 476
Score = 28.7 bits (61), Expect = 2.1
Identities = 19/87 (21%), Positives = 40/87 (45%)
Frame = -1
Query: 374 DYPFIIFWVFPLLPPGFERVLQHFVRSVN*NKIETFVYQHHGVNPCSASTVQHFLASVFF 195
D P ++ + +PP R ++ V N I F+Y +HGV ++ + HF+ + +
Sbjct: 292 DIPHFMY-TYHGVPPNDIRHFRYTYHGVPSNDIPHFMYTYHGV---PSNDIPHFMCT-YH 346
Query: 194 QKPDEEVFIILCSFITVSNEFVPRSSC 114
P ++ + ++ V + +P C
Sbjct: 347 GVPSNDIPHFMYTYHGVPSNDIPHFMC 373
>SB_14110| Best HMM Match : DUF858 (HMM E-Value=2)
Length = 207
Score = 27.9 bits (59), Expect = 3.6
Identities = 18/52 (34%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
Frame = +3
Query: 213 KVLDGACGTGIDSMMLVDEGF-NLVSVDASDKMLKHALKARWEKRKNPKYDE 365
++LD GTG+ + LV GF N+ ++D S+K + A K K Y E
Sbjct: 69 RILDVGSGTGLQAEGLVKHGFTNIDALDPSEKSHEVARKKNLYKNYITDYLE 120
>SB_29100| Best HMM Match : TPMT (HMM E-Value=5.4e-33)
Length = 242
Score = 27.5 bits (58), Expect = 4.8
Identities = 11/34 (32%), Positives = 20/34 (58%)
Frame = +3
Query: 213 KVLDGACGTGIDSMMLVDEGFNLVSVDASDKMLK 314
+VL CG +D + L D+G +V V+ + K ++
Sbjct: 55 RVLLPLCGKSLDLLWLADQGCQVVGVEGASKPIE 88
>SB_3686| Best HMM Match : Myotub-related (HMM E-Value=2.8e-08)
Length = 629
Score = 27.1 bits (57), Expect = 6.3
Identities = 18/87 (20%), Positives = 36/87 (41%), Gaps = 3/87 (3%)
Frame = -1
Query: 353 WVFPLLPPGFERVLQHFVRSVN*NKIETFVYQHHG---VNPCSASTVQHFLASVFFQKPD 183
W+FP + + F + I+ + + + T H + S Q+
Sbjct: 67 WLFPYIEHAIIQHNMEFAEYIKSPSIDNVLLKRRNTPYIEGTLCVTGHHLIFSSRTQR-S 125
Query: 182 EEVFIILCSFITVSNEFVPRSSCLTVC 102
EE+F+++ V +FV + + LT+C
Sbjct: 126 EELFLLISGVEHVEKKFVGQEAILTIC 152
>SB_1712| Best HMM Match : Peptidase_C14 (HMM E-Value=1.6e-21)
Length = 594
Score = 27.1 bits (57), Expect = 6.3
Identities = 10/28 (35%), Positives = 18/28 (64%)
Frame = -1
Query: 233 ASTVQHFLASVFFQKPDEEVFIILCSFI 150
A++V H S+ +KPDE + ++ CS +
Sbjct: 473 ATSVIHLTDSIHPRKPDEMLIVLTCSLV 500
>SB_41291| Best HMM Match : Piwi (HMM E-Value=0)
Length = 598
Score = 27.1 bits (57), Expect = 6.3
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -1
Query: 338 LPPGFERVLQHFVRSVN 288
LPP FE+ +HFVR N
Sbjct: 516 LPPAFEQTTRHFVRGEN 532
>SB_52816| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1622
Score = 26.6 bits (56), Expect = 8.3
Identities = 15/33 (45%), Positives = 20/33 (60%), Gaps = 2/33 (6%)
Frame = +3
Query: 210 KKVLDGACGTGIDSMMLVDEG--FNLVSVDASD 302
K VLD CGTGI S+ EG + +V+AS+
Sbjct: 153 KVVLDVGCGTGILSLFCAREGKASKVYAVEASE 185
>SB_39182| Best HMM Match : Cellulase (HMM E-Value=0.00018)
Length = 949
Score = 26.6 bits (56), Expect = 8.3
Identities = 15/39 (38%), Positives = 21/39 (53%), Gaps = 1/39 (2%)
Frame = -2
Query: 385 LASSITHSSY-FGFFLFSHLALSACFNILSEASTETRLK 272
+AS++ + SY GF LF+ L L C N S + E K
Sbjct: 133 VASTLKNKSYTLGFNLFTELGLDGCGNNCSGSLRENTTK 171
Score = 26.6 bits (56), Expect = 8.3
Identities = 15/39 (38%), Positives = 21/39 (53%), Gaps = 1/39 (2%)
Frame = -2
Query: 385 LASSITHSSY-FGFFLFSHLALSACFNILSEASTETRLK 272
+AS++ + SY GF LF+ L L C N S + E K
Sbjct: 474 VASTLKNKSYTLGFNLFTELGLDGCGNNCSGSLRENTTK 512
Score = 26.6 bits (56), Expect = 8.3
Identities = 15/39 (38%), Positives = 21/39 (53%), Gaps = 1/39 (2%)
Frame = -2
Query: 385 LASSITHSSY-FGFFLFSHLALSACFNILSEASTETRLK 272
+AS++ + SY GF LF+ L L C N S + E K
Sbjct: 780 VASTLKNKSYTLGFNLFTELGLDGCGNNCSGSLRENTTK 818
>SB_23673| Best HMM Match : DUF855 (HMM E-Value=0.52)
Length = 380
Score = 26.6 bits (56), Expect = 8.3
Identities = 25/90 (27%), Positives = 37/90 (41%), Gaps = 6/90 (6%)
Frame = +3
Query: 78 SEGVKDQYADGKAARAWNKFIGDS-----NERTQNYKDFLIGLLKKHGCKKVLDGACGTG 242
S G KD+ G + + DS RT Y++ + K VLD CGTG
Sbjct: 137 SNGAKDKPKPGASEMTSADYYFDSYAHFDEVRTLTYRNSMYHNKHLFRDKIVLDVGCGTG 196
Query: 243 IDSMMLVDEGFNLVSVDASDKMLK-HALKA 329
I SM G V + +++ H L++
Sbjct: 197 ILSMFAAKAGAKHVYAITNTNLIQTHMLES 226
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,244,128
Number of Sequences: 59808
Number of extensions: 263733
Number of successful extensions: 943
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 861
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 936
length of database: 16,821,457
effective HSP length: 75
effective length of database: 12,335,857
effective search space used: 789494848
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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