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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0005_G18
         (512 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC31A8.01c |cwl1|rtn1, SPBC651.13c|reticulon-like protein|Schi...    28   0.71 
SPBC543.07 |pek1|skh1, mkk1|MAP kinase kinase Pek1 |Schizosaccha...    28   0.94 
SPBC1347.01c |rev1|SPBC215.16c|deoxycytidyl transferase Rev1 |Sc...    27   1.2  
SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated memb...    27   2.2  
SPBC16H5.02 |pfk1||6-phosphofructokinase |Schizosaccharomyces po...    26   2.9  
SPAC9.02c |||N-acetyltransferase|Schizosaccharomyces pombe|chr 1...    26   3.8  
SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces ...    25   5.0  
SPAC11D3.13 |||ThiJ domain protein|Schizosaccharomyces pombe|chr...    25   5.0  
SPAC17H9.06c |||conserved fungal protein|Schizosaccharomyces pom...    25   6.7  
SPCC4G3.14 |mdj1||DNAJ domain protein Mdj1 |Schizosaccharomyces ...    25   6.7  
SPAC644.12 |cdc5||cell division control protein Cdc5|Schizosacch...    25   6.7  
SPBC31F10.09c |nut2|med10|mediator complex subunit Med10|Schizos...    25   8.8  
SPAC1D4.02c |||human GRASP protein homolog |Schizosaccharomyces ...    25   8.8  
SPBC11C11.02 |imp2||contractile ring protein Imp2|Schizosaccharo...    25   8.8  
SPBC14F5.12c |cbh2||centromere binding protein Cbh2|Schizosaccha...    25   8.8  
SPAC17A2.03c |vma6||V-type ATPase subunit d|Schizosaccharomyces ...    25   8.8  
SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr ...    25   8.8  

>SPBC31A8.01c |cwl1|rtn1, SPBC651.13c|reticulon-like
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 308

 Score = 28.3 bits (60), Expect = 0.71
 Identities = 15/42 (35%), Positives = 20/42 (47%), Gaps = 1/42 (2%)
 Frame = -3

Query: 150 VTHSGSRRHELSQP-PGIVSRPGSPPCRSRGAPTRRCPRGEA 28
           ++ S S  H  SQ  PG  S P  P   + G   ++CP  EA
Sbjct: 66  ISSSSSEPHHTSQSTPGETSSPVCPVSGAHGGADKKCPALEA 107


>SPBC543.07 |pek1|skh1, mkk1|MAP kinase kinase Pek1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 363

 Score = 27.9 bits (59), Expect = 0.94
 Identities = 31/149 (20%), Positives = 54/149 (36%), Gaps = 1/149 (0%)
 Frame = +3

Query: 18  TSAGLPRGDISESEHLEN-GMAESLDERRFQEAERARVSEILNESRDAPDNEINLDDPCM 194
           TS G     IS  E  +N G+ E  D     E++  +  E  +     P     LDD  +
Sbjct: 11  TSNGFSEEYISHPERNDNQGIVEITDLVFSSESKLTQRKESRDSKTFVPSFLEELDDDHL 70

Query: 195 KVHCSAGRVCEINEHGEAICNCIKECPYETDSRRKVCTNFNETWSSDCEVYRQRCLCLDH 374
               + G +  +N  GE +   +++C       + +        + +  + +Q    L  
Sbjct: 71  HELVTNGGILYMNSLGEGVSGSVRKC--RIRGTQMIFAMKTVLAAPNTALQKQLLRELKI 128

Query: 375 SELCRGAQYHHVQIEYYGTCREMPECSEN 461
           +  C         ++YYG C    EC  N
Sbjct: 129 NRSCTSPYI----VKYYGACYNNAECQLN 153


>SPBC1347.01c |rev1|SPBC215.16c|deoxycytidyl transferase Rev1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 935

 Score = 27.5 bits (58), Expect = 1.2
 Identities = 12/27 (44%), Positives = 17/27 (62%)
 Frame = -2

Query: 322 VSLKFVHTLRLESVSYGHSLMQLQIAS 242
           VS +F H LRL+ V+  H +   +IAS
Sbjct: 291 VSTRFSHELRLKPVAVAHGIKNSEIAS 317


>SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated
           membrane proteins, ESCRT 0 complex|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 610

 Score = 26.6 bits (56), Expect = 2.2
 Identities = 19/65 (29%), Positives = 31/65 (47%)
 Frame = +3

Query: 9   ERPTSAGLPRGDISESEHLENGMAESLDERRFQEAERARVSEILNESRDAPDNEINLDDP 188
           ERP+ A +    IS+ +HL     E L        E A+ S   +++  AP+N +N+  P
Sbjct: 286 ERPSEANVV---ISQDQHLTEDEDEELKRAIAISLEEAQKSSQKDDNVTAPNN-MNISYP 341

Query: 189 CMKVH 203
            +  H
Sbjct: 342 SVPAH 346


>SPBC16H5.02 |pfk1||6-phosphofructokinase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 942

 Score = 26.2 bits (55), Expect = 2.9
 Identities = 12/22 (54%), Positives = 14/22 (63%)
 Frame = +2

Query: 374 LGAVPRGAVPPRADRVLRDLQG 439
           LG V RG +P   DR+L  LQG
Sbjct: 476 LGHVQRGGIPCAYDRMLATLQG 497


>SPAC9.02c |||N-acetyltransferase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 165

 Score = 25.8 bits (54), Expect = 3.8
 Identities = 18/70 (25%), Positives = 27/70 (38%)
 Frame = +3

Query: 42  DISESEHLENGMAESLDERRFQEAERARVSEILNESRDAPDNEINLDDPCMKVHCSAGRV 221
           +I   E  E      L+   F EAERA  + I +  + AP+ ++ L  P        G V
Sbjct: 3   NIRNIERSEVEEVSKLEAICFPEAERASFARIKDRVKRAPEIQLGLFAPTQHTSTLIGHV 62

Query: 222 CEINEHGEAI 251
                  E +
Sbjct: 63  LTSRTGSETV 72


>SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces
            pombe|chr 3|||Manual
          Length = 1502

 Score = 25.4 bits (53), Expect = 5.0
 Identities = 7/23 (30%), Positives = 13/23 (56%)
 Frame = +3

Query: 204  CSAGRVCEINEHGEAICNCIKEC 272
            C+A R+C++ E       C+ +C
Sbjct: 974  CTASRICKVREIASLSLTCLLDC 996


>SPAC11D3.13 |||ThiJ domain protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 222

 Score = 25.4 bits (53), Expect = 5.0
 Identities = 15/49 (30%), Positives = 22/49 (44%)
 Frame = +3

Query: 174 NLDDPCMKVHCSAGRVCEINEHGEAICNCIKECPYETDSRRKVCTNFNE 320
           NL     K++   G +  +  HG  I  CIK+    +  + K  T FNE
Sbjct: 105 NLHKGAAKIYSMGGVIAAVC-HGPVILPCIKDSTGFSIVKGKTVTAFNE 152


>SPAC17H9.06c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 601

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 11/27 (40%), Positives = 19/27 (70%)
 Frame = -2

Query: 187 GSSRLISLSGASRDSFRISETRALSAS 107
           G+ +L     +SRD+FR ++T+A S+S
Sbjct: 47  GNKKLKESQFSSRDNFRTTQTQASSSS 73


>SPCC4G3.14 |mdj1||DNAJ domain protein Mdj1 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 528

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 13/41 (31%), Positives = 21/41 (51%)
 Frame = +1

Query: 205 AAPAVSVRSTSTGKLSATASRSVHTKPTLGARCAQTSTRPG 327
           ++P+ +  STST   S+  S  + T+PT G    Q  +  G
Sbjct: 464 SSPSGTNSSTSTSSTSSKHSTGISTEPTTGEENKQDGSVGG 504


>SPAC644.12 |cdc5||cell division control protein
           Cdc5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 757

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 11/29 (37%), Positives = 15/29 (51%)
 Frame = -3

Query: 405 GGTAPRGTAPSGRGRGSAVGTPRSPMTRS 319
           GGT   G  PS    GSA+  P++   R+
Sbjct: 409 GGTGYTGVTPSHAANGSALAAPQATPFRT 437


>SPBC31F10.09c |nut2|med10|mediator complex subunit
           Med10|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 138

 Score = 24.6 bits (51), Expect = 8.8
 Identities = 10/31 (32%), Positives = 17/31 (54%)
 Frame = +2

Query: 386 PRGAVPPRADRVLRDLQGNAGVQRERNVRLP 478
           P  A+P   D ++RDL+    + R+ N  +P
Sbjct: 34  PSDAIPESLDTLIRDLKSLPDISRKVNNLIP 64


>SPAC1D4.02c |||human GRASP protein homolog |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 345

 Score = 24.6 bits (51), Expect = 8.8
 Identities = 10/26 (38%), Positives = 16/26 (61%)
 Frame = -1

Query: 323 GLVEVCAHLAPRVGFVWTLLDAVADS 246
           G+V   A +AP V  +W +L+ + DS
Sbjct: 104 GMVLQWASIAPAVDAIWHILNVIDDS 129


>SPBC11C11.02 |imp2||contractile ring protein
           Imp2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 670

 Score = 24.6 bits (51), Expect = 8.8
 Identities = 11/31 (35%), Positives = 16/31 (51%)
 Frame = -3

Query: 102 IVSRPGSPPCRSRGAPTRRCPRGEAPPTSAA 10
           + SRP +P   +        PR ++P TSAA
Sbjct: 378 LTSRPSTPNVGNTAPDALSSPRNDSPLTSAA 408


>SPBC14F5.12c |cbh2||centromere binding protein
           Cbh2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 514

 Score = 24.6 bits (51), Expect = 8.8
 Identities = 14/33 (42%), Positives = 19/33 (57%)
 Frame = +3

Query: 78  AESLDERRFQEAERARVSEILNESRDAPDNEIN 176
           A+ LDER F+  E    S+ L  ++DA  N IN
Sbjct: 450 AQFLDEREFETDEEEEESQYLLSTKDA-INAIN 481


>SPAC17A2.03c |vma6||V-type ATPase subunit d|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 343

 Score = 24.6 bits (51), Expect = 8.8
 Identities = 19/74 (25%), Positives = 31/74 (41%), Gaps = 10/74 (13%)
 Frame = +3

Query: 276 YETDSRRKVCTNFNETWS-----SDCEVYRQRCLCLD-----HSELCRGAQYHHVQIEYY 425
           +ET     V TN  E +S     +    Y + CL  D     H E+ R   Y     ++Y
Sbjct: 131 FETLPALCVATNVEELYSVVLIETPLAPYFKDCLSADDLDEQHIEIIRNTLYKAYLEDFY 190

Query: 426 GTCREMPECSENEM 467
             C+++  C+ + M
Sbjct: 191 NFCKKIGACTADTM 204


>SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 422

 Score = 24.6 bits (51), Expect = 8.8
 Identities = 16/56 (28%), Positives = 21/56 (37%)
 Frame = -3

Query: 498 ASRACAAGSLTFRSRCTPAFPCKSRSTRSARGGTAPRGTAPSGRGRGSAVGTPRSP 331
           AS   A  S  + S   P     S +  S+RG T    T P    RG    +  +P
Sbjct: 264 ASSTIAEVSPMYGSHAAPYASTPSAAVGSSRGSTPASATVPISPARGFPTTSAYNP 319


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,085,309
Number of Sequences: 5004
Number of extensions: 42718
Number of successful extensions: 158
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 151
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 157
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 206265012
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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