BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0005_G02 (699 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3H8.10 |spo20|sec14|sec14 cytosolic factor family Sec14|Schi... 42 6e-05 SPAC3H8.02 |||sec14 cytosolic factor family|Schizosaccharomyces ... 40 3e-04 SPAC589.09 |||sec14 cytosolic factor family|Schizosaccharomyces ... 37 0.003 SPBC428.11 |||homocysteine synthase |Schizosaccharomyces pombe|c... 27 2.0 SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|ch... 27 2.6 SPBC3B9.06c |apg3||autophagy associated protein Apg3 |Schizosacc... 26 6.0 SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr... 25 7.9 SPBC17D11.06 |spp2|pri2|DNA primase large subunit Spp2 |Schizosa... 25 7.9 >SPAC3H8.10 |spo20|sec14|sec14 cytosolic factor family Sec14|Schizosaccharomyces pombe|chr 1|||Manual Length = 286 Score = 42.3 bits (95), Expect = 6e-05 Identities = 21/62 (33%), Positives = 31/62 (50%), Gaps = 1/62 (1%) Frame = +2 Query: 263 YPANTYAIHIVNNSWIFDKIYNIFKPLLDNVMRSKIYFHGSDLTS-LHKHIDPDHLPERY 439 YP +++N W F +N+ K LD KI+ GS+ S L + I D+LP + Sbjct: 200 YPERMGKFYVINAPWGFSSAFNLIKGFLDEATVKKIHILGSNYKSALLEQIPADNLPAKL 259 Query: 440 GG 445 GG Sbjct: 260 GG 261 >SPAC3H8.02 |||sec14 cytosolic factor family|Schizosaccharomyces pombe|chr 1|||Manual Length = 444 Score = 40.3 bits (90), Expect = 3e-04 Identities = 30/126 (23%), Positives = 57/126 (45%), Gaps = 1/126 (0%) Frame = +2 Query: 92 EDLFKATILALELGMLEPRTQILGGVALFDLQDLGTQHAWQCTPSVAAKIVKILVSCYPA 271 E + + T+ +E L + I +FD+ D + ++K + YP Sbjct: 211 ESVERLTVWVMETARLILKPPIETATVVFDMTDFSMSNM---DYGPLKFMIKCFEAHYPE 267 Query: 272 NTYAIHIVNNSWIFDKIYNIFKPLLDNVMRSKIYFHGSDLTSLHKHIDPDHLPERYGGIW 451 + W+F +++I K LD V+ SK+ F + L ++I+PD++ + +GG Sbjct: 268 CLGECIVHKAPWLFQGVWSIIKSWLDPVVVSKVKF-TRNYRDLQQYINPDNILKEFGGPN 326 Query: 452 P-DYSY 466 P Y+Y Sbjct: 327 PWRYTY 332 >SPAC589.09 |||sec14 cytosolic factor family|Schizosaccharomyces pombe|chr 1|||Manual Length = 388 Score = 36.7 bits (81), Expect = 0.003 Identities = 24/72 (33%), Positives = 34/72 (47%), Gaps = 4/72 (5%) Frame = +2 Query: 242 VKILVSC----YPANTYAIHIVNNSWIFDKIYNIFKPLLDNVMRSKIYFHGSDLTSLHKH 409 VK L SC YP + + + WIF ++NI K + + +KI F S L K+ Sbjct: 221 VKYLASCLEYYYPQSLGVCILHKSPWIFRSVWNIIKGWIKPEIAAKIVFTQS-ANDLEKY 279 Query: 410 IDPDHLPERYGG 445 ID +P GG Sbjct: 280 IDYSVIPTSLGG 291 >SPBC428.11 |||homocysteine synthase |Schizosaccharomyces pombe|chr 2|||Manual Length = 429 Score = 27.5 bits (58), Expect = 2.0 Identities = 13/43 (30%), Positives = 23/43 (53%) Frame = +2 Query: 287 HIVNNSWIFDKIYNIFKPLLDNVMRSKIYFHGSDLTSLHKHID 415 +IV+ S+++ YN+FK L + + + +G D L ID Sbjct: 104 NIVSTSYLYGGTYNLFKVTLPRLGITTKFVNGDDPNDLAAQID 146 >SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|chr 3|||Manual Length = 611 Score = 27.1 bits (57), Expect = 2.6 Identities = 14/53 (26%), Positives = 25/53 (47%) Frame = +2 Query: 314 DKIYNIFKPLLDNVMRSKIYFHGSDLTSLHKHIDPDHLPERYGGIWPDYSYTI 472 DK + I + + D ++R + G L +HI H ++YG PD + + Sbjct: 261 DKPHEIMEVVEDLLVRLVSFAKGITLAKPFQHITYQHAIDKYGSDKPDIRFEL 313 >SPBC3B9.06c |apg3||autophagy associated protein Apg3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 275 Score = 25.8 bits (54), Expect = 6.0 Identities = 9/21 (42%), Positives = 13/21 (61%) Frame = +3 Query: 99 YLKRRY*LWSWECWNLARKYL 161 YL ++ WSWEC + R +L Sbjct: 42 YLVSKFPTWSWECGDRIRGFL 62 >SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr 3|||Manual Length = 512 Score = 25.4 bits (53), Expect = 7.9 Identities = 7/25 (28%), Positives = 16/25 (64%) Frame = +1 Query: 151 ANTWWRCFVRFARPWHPACLAVHPI 225 A+++W+ F+++ W+ A +H I Sbjct: 239 AHSFWKEFIQYGATWYTAVPTIHQI 263 >SPBC17D11.06 |spp2|pri2|DNA primase large subunit Spp2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 459 Score = 25.4 bits (53), Expect = 7.9 Identities = 13/46 (28%), Positives = 18/46 (39%) Frame = -2 Query: 284 SHRCSLGNMTLKF*QFLLQLMGCTAKHAGCQGLANRTKQRHQVFAC 147 +H C ++ L MG AGC+ + K RH AC Sbjct: 383 AHGCPYRTYSVNNLVSALASMGIAPDSAGCREVVEAVKARHYHIAC 428 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,020,320 Number of Sequences: 5004 Number of extensions: 65827 Number of successful extensions: 172 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 166 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 171 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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