BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0005_F20 (528 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23D3.07 |pup1||20S proteasome component beta 2|Schizosacchar... 191 6e-50 SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyc... 84 1e-17 SPAC4A8.13c |pts1||20S proteasome component beta 5|Schizosacchar... 50 2e-07 SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy... 47 2e-06 SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 44 1e-05 SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosacchar... 35 0.009 SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 33 0.026 SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharom... 32 0.046 SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyce... 32 0.061 SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Sc... 28 0.75 SPBC3H7.13 |||FHA domain protein Far10 |Schizosaccharomyces pomb... 26 3.0 SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki... 26 3.0 SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 26 4.0 SPCC18.18c |fum1|SPCC290.01c|fumarate hydratase|Schizosaccharomy... 25 5.3 SPCC1672.11c |||P-type ATPase |Schizosaccharomyces pombe|chr 3||... 25 7.0 SPBC336.09c |rrn7||RNA polymerase I transcription factor subunit... 25 7.0 SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 25 7.0 SPCC5E4.05c |||serine hydrolase |Schizosaccharomyces pombe|chr 3... 25 7.0 SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 25 9.2 SPAC22H10.07 |scd2|ral3|scaffold protein Scd2|Schizosaccharomyce... 25 9.2 >SPAC23D3.07 |pup1||20S proteasome component beta 2|Schizosaccharomyces pombe|chr 1|||Manual Length = 267 Score = 191 bits (465), Expect = 6e-50 Identities = 87/144 (60%), Positives = 106/144 (73%) Frame = +3 Query: 96 GFSFENCQRNAFLAEKGFPAPKATKTGTTIVGIIYADGVILGADTRATENTVVSDKNCQK 275 GF FE QRN L EKGFP PKAT TGTTIVG+I D ++LGADTRAT +++DKNC+K Sbjct: 9 GFDFEYYQRNLLLQEKGFPTPKATSTGTTIVGVIAKDCIVLGADTRATAGPIIADKNCKK 68 Query: 276 IHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGRTVPVETAATLLKRMLFRYQGH 455 +H ++ N++C GAGTAADTE T ++S +EL L+T R V TA T+LK+ LFRYQGH Sbjct: 69 LHLISPNIWCAGAGTAADTEFVTSMISSNIELHSLYTNRKPRVVTALTMLKQHLFRYQGH 128 Query: 456 IGAALVLGGVDRSGPHIFCIYPHG 527 IGA LVLGG D GPH+F I HG Sbjct: 129 IGAYLVLGGYDCKGPHLFTIAAHG 152 >SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 83.8 bits (198), Expect = 1e-17 Identities = 40/117 (34%), Positives = 66/117 (56%), Gaps = 1/117 (0%) Frame = +3 Query: 168 KTGTTIVGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQ 347 + GTTI + Y DGVIL AD+R T ++++ K+ L N++CC +G+AADT+ Sbjct: 22 RMGTTITALRYKDGVILAADSRTTMGAYIANRVTDKLTQLTDNIWCCRSGSAADTQTVAD 81 Query: 348 SVASQLELQRLHTGRTVPVETAATLLKRMLFRYQGHIGAALVLGGVD-RSGPHIFCI 515 + L + R+ G V TAATL M ++ + + A L++ G D ++G ++ I Sbjct: 82 LLKYYLSMYRIQFGHDPSVHTAATLASEMCYQNKNMLSAGLIVAGYDEKTGGDVYSI 138 >SPAC4A8.13c |pts1||20S proteasome component beta 5|Schizosaccharomyces pombe|chr 1|||Manual Length = 272 Score = 50.4 bits (115), Expect = 2e-07 Identities = 29/121 (23%), Positives = 58/121 (47%), Gaps = 3/121 (2%) Frame = +3 Query: 174 GTTIVGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCG--AGTAADTEMTTQ 347 GTT + Y G+++ D+RA+ +++ + +K+ + N Y G AG AAD + Sbjct: 61 GTTTLAFRYQHGIVVCVDSRASAGPLIASQTVKKV--IEINPYLLGTLAGGAADCQFWET 118 Query: 348 SVASQLELQRLHTGRTVPVETAATLLKRMLFRYQGH-IGAALVLGGVDRSGPHIFCIYPH 524 + + L +L + V A+ +L + + Y+G+ + +L G + G ++ I Sbjct: 119 VLGMECRLHQLRNKELISVSAASKILSNITYSYKGYGLSMGTMLAGTGKGGTALYYIDSD 178 Query: 525 G 527 G Sbjct: 179 G 179 >SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces pombe|chr 1|||Manual Length = 247 Score = 46.8 bits (106), Expect = 2e-06 Identities = 32/136 (23%), Positives = 61/136 (44%), Gaps = 11/136 (8%) Frame = +3 Query: 153 APKATKTGTTIVGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADT 332 A +A K G+T +G+ D V+LG + R T + ++ + +K+ + S++ C +G AD Sbjct: 27 AIEAIKLGSTAIGVKTKDAVVLGVEKRLT-SPLMESHSVEKLFEIDSHIGCAISGLTADA 85 Query: 333 EMTTQSVASQLELQRLHTGRTVPVETAATLLKRMLFRY-QGH----------IGAALVLG 479 + Q + R +E+ + + R+ +G G AL++ Sbjct: 86 RTIIEHARVQTQNHRFTYDEPQGIESTTQSICDLALRFGEGEDGEERIMSRPFGVALLIA 145 Query: 480 GVDRSGPHIFCIYPHG 527 G+D GP ++ P G Sbjct: 146 GIDEHGPQLYHSEPSG 161 >SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 248 Score = 44.4 bits (100), Expect = 1e-05 Identities = 25/91 (27%), Positives = 49/91 (53%), Gaps = 1/91 (1%) Frame = +3 Query: 186 VGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQL 365 +GI+ DG++L A+ + T + +++ +K++++ NM C AG AD + + A ++ Sbjct: 35 LGIVAKDGIVLAAEKKVTSKLLEQEESAEKLYHIGDNMLCAVAGLTADANILI-NYARRV 93 Query: 366 ELQRLHT-GRTVPVETAATLLKRMLFRYQGH 455 Q L T +P E L++R+ QG+ Sbjct: 94 GQQYLQTFNEEMPCE---QLVRRVCDLKQGY 121 >SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosaccharomyces pombe|chr 1|||Manual Length = 225 Score = 34.7 bits (76), Expect = 0.009 Identities = 27/128 (21%), Positives = 58/128 (45%), Gaps = 4/128 (3%) Frame = +3 Query: 156 PKATKTGTTIVGIIYADG-VILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADT 332 P GTT+ I DG IL DTR+ ++ + ++H + ++ +G AD Sbjct: 8 PYVQNGGTTVA--IAGDGFAILAGDTRSVNGYNINTRFQPRVHEVGDDLVIGASGFEADA 65 Query: 333 EMTTQSVASQLELQRLHTGRTVPVETAATLLKRMLF--RYQGHIGAALVLGGVDRSGP-H 503 + + +++L + R + ++ A +++ +L+ R+ + + G+D+ G Sbjct: 66 LALVKRIQQRIDLYHDNHERKMSAQSCACMVRTLLYGKRFFPYY-VYTTVAGIDKEGKGE 124 Query: 504 IFCIYPHG 527 I+ P G Sbjct: 125 IYSFDPVG 132 >SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces pombe|chr 3|||Manual Length = 245 Score = 33.1 bits (72), Expect = 0.026 Identities = 30/149 (20%), Positives = 61/149 (40%), Gaps = 6/149 (4%) Frame = +3 Query: 99 FSFENCQRNAFLAEKGFPAPKATKTGTTIVGIIYADGVILGADTRATENTVVSDKNCQKI 278 FS N L + + A A G T VGI DGV+L + + T + + +K+ Sbjct: 7 FSLTTFSPNGKLVQIEY-ALNAVNAGVTSVGIKATDGVVLATEKKPTSELAIG-ASLEKV 64 Query: 279 HYLASNMYCCGAGTAADTEMTTQS--VASQLELQRLHT----GRTVPVETAATLLKRMLF 440 + ++ +G D + +Q ++++ + + E A+ + + Sbjct: 65 CAITPDIGMVYSGMGPDFRVLVDKSRKVAQTTYKKIYNEYPPTKILVQEIASVMQESTQS 124 Query: 441 RYQGHIGAALVLGGVDRSGPHIFCIYPHG 527 G +L++ G+D GP ++ + P G Sbjct: 125 GGVRPFGVSLLVAGMDEKGPSLYQVDPSG 153 >SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharomyces pombe|chr 3|||Manual Length = 253 Score = 32.3 bits (70), Expect = 0.046 Identities = 30/130 (23%), Positives = 50/130 (38%), Gaps = 5/130 (3%) Frame = +3 Query: 153 APKATKTGTTIVGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADT 332 A KA + +T +GI DGVIL + T + N +I + ++ G D Sbjct: 26 AYKAVENASTCIGIKCEDGVILALEKVVTSKLLKPRVN-NRIGSVDRHIGIATTGFIPDG 84 Query: 333 EMTTQSVASQLELQRLHTGRTVPVETAATLLKR--MLFRYQGHI---GAALVLGGVDRSG 497 + + + R + G +P A L LF + G + D G Sbjct: 85 QHIVKRARDEATSWRDNYGSPIPGTVIADRLGNYVQLFTCYSSVRPFGVMSFVATYDSEG 144 Query: 498 PHIFCIYPHG 527 PH++ + P+G Sbjct: 145 PHLYMVEPNG 154 >SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyces pombe|chr 2|||Manual Length = 262 Score = 31.9 bits (69), Expect = 0.061 Identities = 16/73 (21%), Positives = 39/73 (53%) Frame = +3 Query: 171 TGTTIVGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQS 350 TG++++ + +ADGV++ AD A+ ++ + +++ + N G +D + Q Sbjct: 42 TGSSVLALKFADGVMIAADNLASYGSLARFYDVERLTKVGDNTIVGAGGDISDYQQ-IQR 100 Query: 351 VASQLELQRLHTG 389 + +LE++ + G Sbjct: 101 LLEKLEIKEGNYG 113 >SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Schizosaccharomyces pombe|chr 1|||Manual Length = 272 Score = 28.3 bits (60), Expect = 0.75 Identities = 29/130 (22%), Positives = 52/130 (40%), Gaps = 5/130 (3%) Frame = +3 Query: 153 APKATKTGTTIVGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADT 332 A +A K G+ VG++ +L A R E K K+ + ++ AG A D Sbjct: 25 ALEAIKQGSATVGLVSKTHAVLVALKRNAEELSSYQK---KLIRIDDHIGIAIAGLAPDA 81 Query: 333 EMTTQSVASQLELQRLHTGRTVPVETAAT-LLKRMLFRYQGH----IGAALVLGGVDRSG 497 + + + + + R +PV + + ++ Q + G ++ G D SG Sbjct: 82 RVLSNYMKQEALSSKTLFTRPIPVRRLMSKVAEKAQINTQEYGRRPYGVGFLVIGYDESG 141 Query: 498 PHIFCIYPHG 527 PH+ P G Sbjct: 142 PHLLEFQPSG 151 >SPBC3H7.13 |||FHA domain protein Far10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 301 Score = 26.2 bits (55), Expect = 3.0 Identities = 14/39 (35%), Positives = 22/39 (56%), Gaps = 1/39 (2%) Frame = -1 Query: 357 KQPIVSSSQYLQQYRHHNNTCS-KPSNEFFDNSYRKRQY 244 K+ ++S S + RH N + S PSN FF++ RQ+ Sbjct: 17 KKLVISPSTIYKIGRHTNKSTSPSPSNLFFNSKVLSRQH 55 >SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1436 Score = 26.2 bits (55), Expect = 3.0 Identities = 8/21 (38%), Positives = 16/21 (76%) Frame = +1 Query: 238 LKILSFPIRIVKKFITWLRTC 300 +++LSFP R++K+F+ +C Sbjct: 550 MRLLSFPFRLIKEFLNLRLSC 570 >SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 670 Score = 25.8 bits (54), Expect = 4.0 Identities = 17/70 (24%), Positives = 34/70 (48%) Frame = -2 Query: 323 SSTGTTTIHVRSQVMNFLTILIGNDSIFSSPCIGTKNNAVSVNNPYYCSTSLSSFRSWKP 144 SS+ TTT+ + + + G+ + SSP + ++ + + P S+ SS S+ Sbjct: 316 SSSYTTTLTASNTSVTYTGTGTGSATFTSSPPFYSNSSVIPTSVPSSVSSFTSSNSSYTT 375 Query: 143 FLSEESISLT 114 L+ + S+T Sbjct: 376 TLTASNTSIT 385 Score = 25.4 bits (53), Expect = 5.3 Identities = 16/70 (22%), Positives = 34/70 (48%) Frame = -2 Query: 323 SSTGTTTIHVRSQVMNFLTILIGNDSIFSSPCIGTKNNAVSVNNPYYCSTSLSSFRSWKP 144 +S+ TTT+ + + F G+ + SSP + ++ + + P S+ SS S+ Sbjct: 424 NSSYTTTLTASNTTVTFTGTGTGSATFTSSPPFYSNSSVIPTSAPSSVSSFTSSNSSYTT 483 Query: 143 FLSEESISLT 114 L+ + ++T Sbjct: 484 TLTASNTTVT 493 >SPCC18.18c |fum1|SPCC290.01c|fumarate hydratase|Schizosaccharomyces pombe|chr 3|||Manual Length = 482 Score = 25.4 bits (53), Expect = 5.3 Identities = 9/27 (33%), Positives = 17/27 (62%) Frame = +2 Query: 428 KNVVSLPRTYWGSFSPRRCGQKWSSYL 508 KN++ + RT+ +P GQ++S Y+ Sbjct: 196 KNIIKIGRTHMQDATPLSLGQEFSGYV 222 >SPCC1672.11c |||P-type ATPase |Schizosaccharomyces pombe|chr 3|||Manual Length = 1315 Score = 25.0 bits (52), Expect = 7.0 Identities = 13/38 (34%), Positives = 24/38 (63%), Gaps = 1/38 (2%) Frame = -2 Query: 329 ICSSTGTTTIHVRSQVMNFL-TILIGNDSIFSSPCIGT 219 I S T++I ++ + ++L + L+ +DSIF P +GT Sbjct: 7 IRSKRSTSSIASKNSLNSYLASSLMSHDSIFDGPGLGT 44 >SPBC336.09c |rrn7||RNA polymerase I transcription factor subunit Rrn7 |Schizosaccharomyces pombe|chr 2|||Manual Length = 537 Score = 25.0 bits (52), Expect = 7.0 Identities = 12/39 (30%), Positives = 21/39 (53%) Frame = +1 Query: 19 IKVL*IIQKTYKRPWLPYYYLKYQHQDFRSKTVNEMLSS 135 +K+ +QK Y R +P++Y YQ T+ ++L S Sbjct: 223 LKIFKRLQKNYVRMLIPFHYPTYQRIQSAVLTLVDVLVS 261 >SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1|||Manual Length = 1841 Score = 25.0 bits (52), Expect = 7.0 Identities = 14/33 (42%), Positives = 18/33 (54%) Frame = -1 Query: 390 GRYVNAAVRVAKQPIVSSSQYLQQYRHHNNTCS 292 G Y+N VR AK + S Q L ++ NNT S Sbjct: 1201 GNYMNDYVRQAKGFAIGSLQRLPLIKNANNTKS 1233 >SPCC5E4.05c |||serine hydrolase |Schizosaccharomyces pombe|chr 3|||Manual Length = 378 Score = 25.0 bits (52), Expect = 7.0 Identities = 10/23 (43%), Positives = 15/23 (65%) Frame = -1 Query: 306 NNTCSKPSNEFFDNSYRKRQYFQ 238 N TCS S EF++N+ K + +Q Sbjct: 220 NVTCSDSSKEFYENAGTKDKTYQ 242 >SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharomyces pombe|chr 1|||Manual Length = 969 Score = 24.6 bits (51), Expect = 9.2 Identities = 9/19 (47%), Positives = 11/19 (57%) Frame = -3 Query: 409 VSTGTVRPVCKRCSSSCEA 353 V T RPVC C+ +C A Sbjct: 62 VQTSDGRPVCSSCAHTCTA 80 >SPAC22H10.07 |scd2|ral3|scaffold protein Scd2|Schizosaccharomyces pombe|chr 1|||Manual Length = 536 Score = 24.6 bits (51), Expect = 9.2 Identities = 9/27 (33%), Positives = 17/27 (62%) Frame = +3 Query: 309 GAGTAADTEMTTQSVASQLELQRLHTG 389 G+G + T++TT S ++ + LH+G Sbjct: 96 GSGQISFTDLTTNSSTTRSSISELHSG 122 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,175,622 Number of Sequences: 5004 Number of extensions: 42314 Number of successful extensions: 149 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 142 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 146 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 216376042 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
- SilkBase 1999-2023 -