Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0005_F12
(540 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g57290.1 68416.m06377 eukaryotic translation initiation facto... 120 5e-28
At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)... 31 0.65
At2g37080.1 68415.m04550 myosin heavy chain-related low similari... 28 3.5
At1g78960.1 68414.m09206 lupeol synthase, putative / 2,3-oxidosq... 28 3.5
At5g52910.1 68418.m06566 timeless family protein contains Pfam d... 27 6.1
At5g07890.1 68418.m00910 myosin heavy chain-related contains wea... 27 6.1
At3g53350.3 68416.m05888 myosin heavy chain-related low similari... 27 6.1
At3g53350.2 68416.m05887 myosin heavy chain-related low similari... 27 6.1
At3g53350.1 68416.m05886 myosin heavy chain-related low similari... 27 6.1
At1g52410.2 68414.m05915 caldesmon-related weak similarity to Ca... 27 6.1
At1g52410.1 68414.m05914 caldesmon-related weak similarity to Ca... 27 6.1
At4g27180.1 68417.m03904 kinesin-like protein B (KATB) 27 8.0
At3g61140.1 68416.m06842 COP9 signalosome complex subunit 1 / CS... 27 8.0
At2g46810.1 68415.m05841 basic helix-loop-helix (bHLH) family pr... 27 8.0
At1g51730.1 68414.m05829 RWD domain-containing protein contains ... 27 8.0
>At3g57290.1 68416.m06377 eukaryotic translation initiation factor
3E / eIF3e (TIF3E1) identical to eukaryotic initiation
factor 3E subunit [Arabidopsis thaliana]
gi|12407658|gb|AAG53613
Length = 441
Score = 120 bits (290), Expect = 5e-28
Identities = 57/155 (36%), Positives = 96/155 (61%)
Frame = +2
Query: 74 SYSKFDLTFKIGQYLDRHLVFPLLEFLAAKETYDQSELLQAKLEILSKTNMIDYVIDIRR 253
S +DLT I LDRHLVFP+ EFL ++ Y ++L++K+++L++TNM+DY +DI +
Sbjct: 4 SKQNYDLTPLIAPNLDRHLVFPIFEFLQERQLYPDEQILKSKIQLLNQTNMVDYAMDIHK 63
Query: 254 MLYPDEDTPEEIKSRRGVVLSELQELQDAVEPVLRLMQRDDVMKTVETMRDPKTLINHLT 433
LY ED P+E+ RR V++ L+ L++A P++ + + V+ +R K N
Sbjct: 64 SLYHTEDAPQEMVERRTEVVARLKSLEEAAAPLVSFLLNPN---AVQELRADKQ-YNLQM 119
Query: 434 TNKEYEFKIEMIDCMYRLAKYRYECGNYVESASYL 538
+ Y+ + I+ +Y+ AK+++ECGNY +A YL
Sbjct: 120 LKERYQIGPDQIEALYQYAKFQFECGNYSGAADYL 154
>At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)
Similar to Arabidopsis luminal binding protein
(gb|D89342); contains Pfam domain PF00012: dnaK protein
Length = 678
Score = 30.7 bits (66), Expect = 0.65
Identities = 29/115 (25%), Positives = 53/115 (46%), Gaps = 5/115 (4%)
Frame = +2
Query: 158 AKETYDQSELLQAKLEILSKT-----NMIDYVIDIRRMLYPDEDTPEEIKSRRGVVLSEL 322
A+E ++ ++++ K++ +K NM V D ++ D E+ + GV+ L
Sbjct: 567 AEEFAEEDKIMKEKIDARNKLETYVYNMKSTVADKEKLAKKISD--EDKEKMEGVLKEAL 624
Query: 323 QELQDAVEPVLRLMQRDDVMKTVETMRDPKTLINHLTTNKEYEFKIEMIDCMYRL 487
+ L++ V D+ +K VE + DP + T E E +EMI YR+
Sbjct: 625 EWLEENVNAEKE--DYDEKLKEVELVCDPVIKSVYEKTEGENEDTMEMITMSYRI 677
>At2g37080.1 68415.m04550 myosin heavy chain-related low similarity
to myosin heavy chain [Rana catesbeiana] GI:4249701
Length = 583
Score = 28.3 bits (60), Expect = 3.5
Identities = 19/75 (25%), Positives = 35/75 (46%), Gaps = 3/75 (4%)
Frame = +2
Query: 179 SELLQAKLEILSKTNMIDYVID---IRRMLYPDEDTPEEIKSRRGVVLSELQELQDAVEP 349
+EL Q+K E+ S ++ + + R D + EE+K V E+ +L+ AVE
Sbjct: 249 TELEQSKSEVRSLEQLVRQLEEEDEARGNANGDSSSVEELKEEINVARQEISQLKSAVEV 308
Query: 350 VLRLMQRDDVMKTVE 394
R + + T++
Sbjct: 309 TERRYHEEYIQSTLQ 323
>At1g78960.1 68414.m09206 lupeol synthase, putative /
2,3-oxidosqualene-triterpenoid cyclase, putative similar
to lupeol synthase GI:1762150 from [Arabidopsis
thaliana], 2,3-oxidosqualene-triterpenoid cyclase
[Arabidopsis thaliana] GI:2738027
Length = 763
Score = 28.3 bits (60), Expect = 3.5
Identities = 19/65 (29%), Positives = 31/65 (47%), Gaps = 1/65 (1%)
Frame = +2
Query: 146 EFLAAKETYDQSELLQAKLEILSKTNMIDYVIDIRRMLYPDEDTPEEIKS-RRGVVLSEL 322
E+L D + A+ E + T+ + + + + LYPD T E IKS +GV E
Sbjct: 542 EWLELLNPTDFFTCVMAEREYVECTSAVIQALVLFKQLYPDHRTKEIIKSIEKGVQFIES 601
Query: 323 QELQD 337
++ D
Sbjct: 602 KQTPD 606
>At5g52910.1 68418.m06566 timeless family protein contains Pfam
domains PF05029: Timeless protein C terminal region and
PF04821: Timeless protein
Length = 1141
Score = 27.5 bits (58), Expect = 6.1
Identities = 13/37 (35%), Positives = 22/37 (59%)
Frame = +2
Query: 266 DEDTPEEIKSRRGVVLSELQELQDAVEPVLRLMQRDD 376
D PEE + RR + S+ + D ++ +LR ++RDD
Sbjct: 24 DLGLPEEDEDRRRIGYSKSEYCLDNLKDLLRFLRRDD 60
>At5g07890.1 68418.m00910 myosin heavy chain-related contains weak
similarity to Myosin heavy chain, cardiac muscle alpha
isoform (MyHC-alpha) (Alpha isomyosin) (Fragment)
(Swiss-Prot:P04460) [Oryctolagus cuniculus]
Length = 409
Score = 27.5 bits (58), Expect = 6.1
Identities = 14/61 (22%), Positives = 30/61 (49%), Gaps = 1/61 (1%)
Frame = +2
Query: 182 ELLQAKLEILSKTNMIDYVIDIRRMLYPDEDTPEEIKSR-RGVVLSELQELQDAVEPVLR 358
+ + KLE+ N+ID V + + ++ ED +++K + L +E +D + +
Sbjct: 298 DAIMKKLELSQNVNLIDKVEGMGKQIHQHEDVVKQLKEELKQEKLKAKEEAEDLTQEMAE 357
Query: 359 L 361
L
Sbjct: 358 L 358
>At3g53350.3 68416.m05888 myosin heavy chain-related low similarity
to filamin-interacting protein S-FILIP [Rattus
norvegicus] GI:21392397, nonmuscle heavy chain myosin
II-A [Mus musculus] GI:17978023
Length = 394
Score = 27.5 bits (58), Expect = 6.1
Identities = 21/84 (25%), Positives = 40/84 (47%), Gaps = 3/84 (3%)
Frame = +2
Query: 152 LAAKETYDQSELLQAKLEILSKTNMIDYVIDIRRMLYPDEDTP---EEIKSRRGVVLSEL 322
L +K +SEL Q+K E+ S ++ ++ R+ D + EE+K + E+
Sbjct: 166 LKSKLFESESELEQSKYEVRSLEKLVRQ-LEEERVNSRDSSSSMEVEELKEAMNLSRQEI 224
Query: 323 QELQDAVEPVLRLMQRDDVMKTVE 394
+L+ AVE Q + + T++
Sbjct: 225 TQLKSAVEAAETRYQEEYIQSTLQ 248
>At3g53350.2 68416.m05887 myosin heavy chain-related low similarity
to filamin-interacting protein S-FILIP [Rattus
norvegicus] GI:21392397, nonmuscle heavy chain myosin
II-A [Mus musculus] GI:17978023
Length = 394
Score = 27.5 bits (58), Expect = 6.1
Identities = 21/84 (25%), Positives = 40/84 (47%), Gaps = 3/84 (3%)
Frame = +2
Query: 152 LAAKETYDQSELLQAKLEILSKTNMIDYVIDIRRMLYPDEDTP---EEIKSRRGVVLSEL 322
L +K +SEL Q+K E+ S ++ ++ R+ D + EE+K + E+
Sbjct: 166 LKSKLFESESELEQSKYEVRSLEKLVRQ-LEEERVNSRDSSSSMEVEELKEAMNLSRQEI 224
Query: 323 QELQDAVEPVLRLMQRDDVMKTVE 394
+L+ AVE Q + + T++
Sbjct: 225 TQLKSAVEAAETRYQEEYIQSTLQ 248
>At3g53350.1 68416.m05886 myosin heavy chain-related low similarity
to filamin-interacting protein S-FILIP [Rattus
norvegicus] GI:21392397, nonmuscle heavy chain myosin
II-A [Mus musculus] GI:17978023
Length = 396
Score = 27.5 bits (58), Expect = 6.1
Identities = 21/84 (25%), Positives = 40/84 (47%), Gaps = 3/84 (3%)
Frame = +2
Query: 152 LAAKETYDQSELLQAKLEILSKTNMIDYVIDIRRMLYPDEDTP---EEIKSRRGVVLSEL 322
L +K +SEL Q+K E+ S ++ ++ R+ D + EE+K + E+
Sbjct: 168 LKSKLFESESELEQSKYEVRSLEKLVRQ-LEEERVNSRDSSSSMEVEELKEAMNLSRQEI 226
Query: 323 QELQDAVEPVLRLMQRDDVMKTVE 394
+L+ AVE Q + + T++
Sbjct: 227 TQLKSAVEAAETRYQEEYIQSTLQ 250
>At1g52410.2 68414.m05915 caldesmon-related weak similarity to
Caldesmon (CDM) (Swiss-Prot:P12957) [Gallus gallus]
Length = 759
Score = 27.5 bits (58), Expect = 6.1
Identities = 21/73 (28%), Positives = 36/73 (49%), Gaps = 1/73 (1%)
Frame = +2
Query: 164 ETYDQSELL-QAKLEILSKTNMIDYVIDIRRMLYPDEDTPEEIKSRRGVVLSELQELQDA 340
E+ +S LL Q KLE + N I+ + +D EE+ + R +L E++ +A
Sbjct: 97 ESVGRSSLLDQIKLEFEAHHNSINQA-GSDGVKAESKDDDEELSAHRQKMLEEIEHEFEA 155
Query: 341 VEPVLRLMQRDDV 379
L+ ++ DDV
Sbjct: 156 ASDSLKQLKTDDV 168
>At1g52410.1 68414.m05914 caldesmon-related weak similarity to
Caldesmon (CDM) (Swiss-Prot:P12957) [Gallus gallus]
Length = 755
Score = 27.5 bits (58), Expect = 6.1
Identities = 21/73 (28%), Positives = 36/73 (49%), Gaps = 1/73 (1%)
Frame = +2
Query: 164 ETYDQSELL-QAKLEILSKTNMIDYVIDIRRMLYPDEDTPEEIKSRRGVVLSELQELQDA 340
E+ +S LL Q KLE + N I+ + +D EE+ + R +L E++ +A
Sbjct: 97 ESVGRSSLLDQIKLEFEAHHNSINQA-GSDGVKAESKDDDEELSAHRQKMLEEIEHEFEA 155
Query: 341 VEPVLRLMQRDDV 379
L+ ++ DDV
Sbjct: 156 ASDSLKQLKTDDV 168
>At4g27180.1 68417.m03904 kinesin-like protein B (KATB)
Length = 745
Score = 27.1 bits (57), Expect = 8.0
Identities = 16/46 (34%), Positives = 26/46 (56%)
Frame = +2
Query: 269 EDTPEEIKSRRGVVLSELQELQDAVEPVLRLMQRDDVMKTVETMRD 406
+DT E++S+ V E++ELQD + R +Q D + T E M +
Sbjct: 301 KDTINELESKCSVQNKEIEELQDQLVASERKLQVAD-LSTFEKMNE 345
>At3g61140.1 68416.m06842 COP9 signalosome complex subunit 1 / CSN
complex subunit 1 (CSN1) / COP11 protein (COP11) / FUSCA
protein (FUS6) FUSCA6, COP11, CSN1; identical to FUS6
GI:432446, SP:P45432 from [Arabidopsis thaliana];
contains Pfam profile PF01399: PCI domain; identical to
cDNA CSN complex subunit 1 (CSN1) GI:18056652
Length = 441
Score = 27.1 bits (57), Expect = 8.0
Identities = 11/36 (30%), Positives = 23/36 (63%)
Frame = +2
Query: 152 LAAKETYDQSELLQAKLEILSKTNMIDYVIDIRRML 259
L A ++D+SEL Q ++ ++ N ++ V D+R ++
Sbjct: 264 LCALASFDRSELKQKVIDNINFRNFLELVPDVRELI 299
>At2g46810.1 68415.m05841 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain
Length = 371
Score = 27.1 bits (57), Expect = 8.0
Identities = 25/101 (24%), Positives = 47/101 (46%), Gaps = 6/101 (5%)
Frame = +2
Query: 173 DQSELLQAKLEILSKTNMIDYVIDIRRMLYPDEDTPEEIKS----RRGVVLSELQELQDA 340
+QS L +++ + +D +++RR L E T E+ +S LS LQD
Sbjct: 8 NQSFKLHQQVQCKDEIFCLDQKVNVRRSLQVQE-TVEDHQSFALEEEEQQLSTPSLLQDT 66
Query: 341 VEPVLRLMQRDDVMKTVETMRDPK--TLINHLTTNKEYEFK 457
P L+++Q+ + + +DP L++ T K +E +
Sbjct: 67 TIPFLQMLQQSEDPSPFLSFKDPSFLALLSLQTLEKPWELE 107
>At1g51730.1 68414.m05829 RWD domain-containing protein contains
Pfam profile PF05773: RWD domain; similar to GCN2
eIF2alpha kinase (GI:6066585) [Mus musculus]; similar to
GCN2beta (GI:10764163) [Mus musculus]; similar to RING
finger protein 25 (RING finger protein AO7)
(Swiss-Prot:Q9QZR0) [Mus musculus]; similar to RING
finger protein 25 (Swiss-Prot:Q96BH1) [Homo sapiens]
Length = 252
Score = 27.1 bits (57), Expect = 8.0
Identities = 12/29 (41%), Positives = 20/29 (68%)
Frame = +2
Query: 260 YPDEDTPEEIKSRRGVVLSELQELQDAVE 346
YPDE ++KS RG+ +S+L L++ +E
Sbjct: 71 YPDEAPLLDVKSIRGIHVSDLTILKEKLE 99
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,757,470
Number of Sequences: 28952
Number of extensions: 173448
Number of successful extensions: 443
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 436
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 442
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1003808112
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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