BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0005_F10 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC970.01 |rad16|rad10, rad20, swi9|DNA repair endonuclease XPF... 29 0.55 SPCC320.13c |ark1|aim1, SPCC330.16|aurora-B kinase Ark1|Schizosa... 28 0.95 SPBC428.13c |mob1||protein kinase regulator Mob1|Schizosaccharom... 27 1.3 SPAC458.07 |tfa1|SPAPYUG7.01|transcription factor TFIIE alpha su... 26 2.9 SPCC1393.02c |||non-specific DNA binding protein Spt2 |Schizosac... 26 3.8 SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 25 5.1 SPBC18H10.20c |||conserved fungal protein|Schizosaccharomyces po... 25 5.1 SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr ... 25 6.7 SPBC365.15 |alp4||gamma tubulin complex Spc97/GCP2 subunit Alp4|... 25 8.9 >SPCC970.01 |rad16|rad10, rad20, swi9|DNA repair endonuclease XPF|Schizosaccharomyces pombe|chr 3|||Manual Length = 892 Score = 28.7 bits (61), Expect = 0.55 Identities = 18/98 (18%), Positives = 43/98 (43%), Gaps = 1/98 (1%) Frame = +2 Query: 86 PTREYRRQEDRVYSSPKTQTQSASINDQIEREIIIDSLFVKHLSPNNGQTKNIQRVTTVD 265 P + RR ++ +T + + ND R++ ++ + + HLS +D Sbjct: 464 PPSKRRRVRGGNNATSRTTSDNTDANDSFSRDLRLEKILLSHLSKRYEPEVGNDAFEVID 523 Query: 266 PMVTT-TAATNGDRRHCQCVPYDHCNINYVVIYENDPD 376 + + NG+R + ++ YV+++++DP+ Sbjct: 524 DFNSIYIYSYNGERDE---LVLNNLRPRYVIMFDSDPN 558 >SPCC320.13c |ark1|aim1, SPCC330.16|aurora-B kinase Ark1|Schizosaccharomyces pombe|chr 3|||Manual Length = 355 Score = 27.9 bits (59), Expect = 0.95 Identities = 12/26 (46%), Positives = 19/26 (73%) Frame = +3 Query: 108 KKTGFIAALKPRHKAPV*MTKSKEKL 185 KKTGFI ALK HK+ + +K ++++ Sbjct: 109 KKTGFIVALKTLHKSELVQSKIEKQV 134 >SPBC428.13c |mob1||protein kinase regulator Mob1|Schizosaccharomyces pombe|chr 2|||Manual Length = 210 Score = 27.5 bits (58), Expect = 1.3 Identities = 10/31 (32%), Positives = 19/31 (61%) Frame = +2 Query: 83 GPTREYRRQEDRVYSSPKTQTQSASINDQIE 175 GP+ EY Q+D++Y+ P + IN+ ++ Sbjct: 88 GPSYEYYWQDDKIYTKPTRMSAPDYINNLLD 118 >SPAC458.07 |tfa1|SPAPYUG7.01|transcription factor TFIIE alpha subunit Tfa1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 448 Score = 26.2 bits (55), Expect = 2.9 Identities = 10/26 (38%), Positives = 18/26 (69%) Frame = +2 Query: 224 NGQTKNIQRVTTVDPMVTTTAATNGD 301 N +TK+I+ ++ P+V+T T+GD Sbjct: 392 NKRTKSIEENNSLPPIVSTNGITDGD 417 >SPCC1393.02c |||non-specific DNA binding protein Spt2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 406 Score = 25.8 bits (54), Expect = 3.8 Identities = 26/109 (23%), Positives = 43/109 (39%), Gaps = 8/109 (7%) Frame = +2 Query: 44 KVTMSDETLVNLY-GPTREYRRQEDRVYSSPKTQTQSASINDQIEREII-------IDSL 199 K + LV L GP R+ +R V + Q++SI+ + + S Sbjct: 224 KARYASNGLVQLQTGPKRD-KRSAGEVQDEIMKRRQNSSISQAATPRTVSNSETSYVGSP 282 Query: 200 FVKHLSPNNGQTKNIQRVTTVDPMVTTTAATNGDRRHCQCVPYDHCNIN 346 +K PN+ ++ N R T+ +T A RH + V D +N Sbjct: 283 ALKQSKPNSLKSNNTSRKTSASSAITKPKARPHTSRHDEFVVSDDDELN 331 >SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1647 Score = 25.4 bits (53), Expect = 5.1 Identities = 12/46 (26%), Positives = 21/46 (45%) Frame = +2 Query: 32 TRSYKVTMSDETLVNLYGPTREYRRQEDRVYSSPKTQTQSASINDQ 169 + SY+ T SD + + + +Y Q+D VY T N++ Sbjct: 186 SNSYETTYSDSSNYHTSTDSSQYNDQDDHVYDDTNDGTDDDINNNE 231 >SPBC18H10.20c |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 361 Score = 25.4 bits (53), Expect = 5.1 Identities = 12/22 (54%), Positives = 15/22 (68%) Frame = -3 Query: 97 FTCRPIEIYQCFVRHRDLVRPR 32 F+C+P E RHRDLV+PR Sbjct: 230 FSCQPCE------RHRDLVKPR 245 >SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1337 Score = 25.0 bits (52), Expect = 6.7 Identities = 13/44 (29%), Positives = 21/44 (47%) Frame = +2 Query: 170 IEREIIIDSLFVKHLSPNNGQTKNIQRVTTVDPMVTTTAATNGD 301 ++ + + D L V ++S N QT N P+V T+GD Sbjct: 1201 LDHKFVADQLAVLYMSSNALQTGNPLPQVVPSPLVDRFFTTSGD 1244 >SPBC365.15 |alp4||gamma tubulin complex Spc97/GCP2 subunit Alp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 784 Score = 24.6 bits (51), Expect = 8.9 Identities = 12/36 (33%), Positives = 21/36 (58%) Frame = +2 Query: 362 ENDPDITGTGRIDIRFDDDGCQENMKLCCRIPKPLT 469 E++ GT ++ D +G E M+LC ++P PL+ Sbjct: 543 EHNRSTQGTSKVGSDKDINGF-ETMQLCYKVPFPLS 577 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,932,705 Number of Sequences: 5004 Number of extensions: 38104 Number of successful extensions: 92 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 90 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 92 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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