Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0005_F08
(545 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g45220.1 68416.m04880 serpin, putative / serine protease inhi... 62 2e-10
At2g26390.1 68415.m03167 serpin, putative / serine protease inhi... 61 4e-10
At2g25240.1 68415.m03020 serpin, putative / serine protease inhi... 58 3e-09
At1g64030.1 68414.m07252 serpin family protein / serine protease... 56 2e-08
At1g47710.1 68414.m05302 serpin, putative / serine protease inhi... 55 4e-08
At2g35580.1 68415.m04357 serpin family protein / serine protease... 43 2e-04
At1g63280.1 68414.m07154 serpin-related / serine protease inhibi... 39 0.003
At1g62170.1 68414.m07013 serpin family protein / serine protease... 36 0.013
At1g51330.1 68414.m05772 serpin-related / serine protease inhibi... 36 0.023
At3g02280.1 68416.m00209 flavodoxin family protein low similarit... 29 2.7
At1g70900.1 68414.m08181 expressed protein 29 2.7
At1g23110.1 68414.m02889 hypothetical protein 29 2.7
At5g19010.1 68418.m02258 mitogen-activated protein kinase, putat... 28 4.7
At5g14950.1 68418.m01754 glycosyl hydrolase family 38 protein si... 28 4.7
At4g01290.1 68417.m00170 expressed protein 28 4.7
At3g25140.1 68416.m03139 glycosyl transferase family 8 protein c... 28 4.7
At5g35940.1 68418.m04325 jacalin lectin family protein similar t... 27 6.2
At3g55340.1 68416.m06146 RNA recognition motif (RRM)-containing ... 27 6.2
At3g13170.1 68416.m01647 DNA topoisomerase VIA (SPO11-1) identic... 27 8.2
At2g23990.2 68415.m02866 plastocyanin-like domain-containing pro... 27 8.2
At2g23990.1 68415.m02865 plastocyanin-like domain-containing pro... 27 8.2
>At3g45220.1 68416.m04880 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 393
Score = 62.5 bits (145), Expect = 2e-10
Identities = 49/173 (28%), Positives = 82/173 (47%), Gaps = 9/173 (5%)
Frame = +1
Query: 49 TSSKDKNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKF--LGNGDY-SGVANPYISLSKTF 219
T + N+V SP+ + +L+ L AG+ ++ +I F L + DY + V +S++
Sbjct: 24 TVANGSNLVFSPMSINVLLCLIAAGSNCVTKEQILSFIMLPSSDYLNAVLAKTVSVALND 83
Query: 220 SEMNPD-YFTMANKIYVGNKYTLDEKFT-ITVRQYQSEVETIDFSDTKKAADI--INQWA 387
D + + A +++ + F + Y + +DF+ TK A I +N WA
Sbjct: 84 GMERSDLHLSTAYGVWIDKSLSFKPSFKDLLENSYNATCNQVDFA-TKPAEVINEVNAWA 142
Query: 388 NEKTRGHIKSPISDDTIDP--NAAAALFNVIFFQGHWHVPFNASETKEKDFML 540
T G IK +SDD+I + L N ++F+G W F+A TK DF L
Sbjct: 143 EVHTNGLIKEILSDDSIKTIRESMLILANAVYFKGAWSKKFDAKLTKSYDFHL 195
>At2g26390.1 68415.m03167 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 389
Score = 61.3 bits (142), Expect = 4e-10
Identities = 43/165 (26%), Positives = 76/165 (46%), Gaps = 7/165 (4%)
Frame = +1
Query: 67 NVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKTFSEMNPDYFT 246
NVV SP+ + +L+ L AG+ ++ EI FL + + ++ +E + +
Sbjct: 31 NVVFSPMSINVLLSLIAAGSNPVTKEEILSFLMSPSTDHLNAVLAKIADGGTERSDLCLS 90
Query: 247 MANKIYVGNKYTLDEKFT-ITVRQYQSEVETIDFSDTK-KAADIINQWANEKTRGHIKSP 420
A+ +++ L F + Y++ +DF+ + D +N WA+ T G IK
Sbjct: 91 TAHGVWIDKSSYLKPSFKELLENSYKASCSQVDFATKPVEVIDEVNIWADVHTNGLIKQI 150
Query: 421 ISDDTIDP-----NAAAALFNVIFFQGHWHVPFNASETKEKDFML 540
+S D D N+ L N ++F+ W F+A TK+ DF L
Sbjct: 151 LSRDCTDTIKEIRNSTLILANAVYFKAAWSRKFDAKLTKDNDFHL 195
>At2g25240.1 68415.m03020 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 324
Score = 58.4 bits (135), Expect = 3e-09
Identities = 32/103 (31%), Positives = 55/103 (53%), Gaps = 4/103 (3%)
Frame = +1
Query: 244 TMANKIYVGNKYTLDEKFT-ITVRQYQSEVETIDF-SDTKKAADIINQWANEKTRGHIKS 417
++AN +++ ++L F + Y++ +DF S + D +N WA T G IK
Sbjct: 28 SIANGVWIDKFFSLKLSFKDLLENSYKATCSQVDFASKPSEVIDEVNTWAEVHTNGLIKQ 87
Query: 418 PISDDTIDP--NAAAALFNVIFFQGHWHVPFNASETKEKDFML 540
+S D+ID ++ L N ++F+G W F+A+ TK+ DF L
Sbjct: 88 ILSRDSIDTIRSSTLVLANAVYFKGAWSSKFDANMTKKNDFHL 130
>At1g64030.1 68414.m07252 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
[Cucurbita maxima] GI:9937311, serpin [Triticum
aestivum] GI:871551; contains Pfam profile PF00079:
Serpin (serine protease inhibitor)
Length = 385
Score = 55.6 bits (128), Expect = 2e-08
Identities = 40/171 (23%), Positives = 69/171 (40%), Gaps = 7/171 (4%)
Frame = +1
Query: 49 TSSKDKNVVSSPLGVMMLMLLYKAG-AGEGSRAEIDKFLGNGDYSGVANPYISLSKTF-- 219
++ KD NV+ SP + + ++ AG G+ +I FL + + + L+
Sbjct: 24 SAPKDSNVIFSPASINSAITMHAAGPGGDLVSGQILSFLRSSSIDELKTVFRELASVVYA 83
Query: 220 --SEMNPDYFTMANKIYVGNKYTLDEKFTITVRQYQSEVET-IDF-SDTKKAADIINQWA 387
S T AN +++ D KF + V +DF S+ ++ +N W
Sbjct: 84 DRSATGGPKITAANGLWIDKSLPTDPKFKDLFENFFKAVYVPVDFRSEAEEVRKEVNSWV 143
Query: 388 NEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASETKEKDFML 540
T IK + D ++ N + F+G W PF T++ DF L
Sbjct: 144 EHHTNNLIKDLLPDGSVTSLTNKIYANALSFKGAWKRPFEKYYTRDNDFYL 194
>At1g47710.1 68414.m05302 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 391
Score = 54.8 bits (126), Expect = 4e-08
Identities = 41/185 (22%), Positives = 83/185 (44%), Gaps = 9/185 (4%)
Frame = +1
Query: 13 DKTSLQFLKETYTS-SKDKNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFL---GNGDYS 180
++ S+ K T+ S++ NV+ SP + +++ + AG+ ++ +I FL +
Sbjct: 11 NQVSMNLAKHVITTVSQNSNVIFSPASINVVLSIIAAGSAGATKDQILSFLKFSSTDQLN 70
Query: 181 GVANPYISLSKTFSEMNPD-YFTMANKIYVGNKYTLDEKFTITVRQ-YQSEVETIDFSDT 354
++ +S N ++AN ++ + F + Y++ DF
Sbjct: 71 SFSSEIVSAVLADGSANGGPKLSVANGAWIDKSLSFKPSFKQLLEDSYKAASNQADFQS- 129
Query: 355 KKAADII---NQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASETKE 525
KA ++I N WA ++T G I + + + D N ++F+G W+ F+ S T+E
Sbjct: 130 -KAVEVIAEVNSWAEKETNGLITEVLPEGSADSMTKLIFANALYFKGTWNEKFDESLTQE 188
Query: 526 KDFML 540
+F L
Sbjct: 189 GEFHL 193
>At2g35580.1 68415.m04357 serpin family protein / serine protease
inhibitor family protein similar to protein zx [Hordeum
vulgare subsp. vulgare] GI:19071, serpin [Triticum
aestivum] GI:1885350; contains Pfam profile PF00079:
Serpin (serine protease inhibitor)
Length = 374
Score = 42.7 bits (96), Expect = 2e-04
Identities = 26/102 (25%), Positives = 48/102 (47%), Gaps = 5/102 (4%)
Frame = +1
Query: 250 ANKIYVGNKYTLDEKFT-ITVRQYQSEVETIDFSDTKKAADI---INQWANEKTRGHIKS 417
AN +++ ++ F + + Y++ +DF KA ++ +N W ++T G I +
Sbjct: 95 ANGLWIEKTLNVEPSFKDLLLNSYKAAFNRVDFRT--KADEVNREVNSWVEKQTNGLITN 152
Query: 418 PISDDTIDPNAAAALF-NVIFFQGHWHVPFNASETKEKDFML 540
+ + +F N +FF G W F+ S TK+ DF L
Sbjct: 153 LLPSNPKSAPLTDHIFANALFFNGRWDSQFDPSLTKDSDFHL 194
>At1g63280.1 68414.m07154 serpin-related / serine protease
inhibitor-related similar to protein zx [Hordeum vulgare
subsp. vulgare] GI:19071, serpin [Triticum aestivum]
GI:1885346
Length = 120
Score = 38.7 bits (86), Expect = 0.003
Identities = 15/54 (27%), Positives = 28/54 (51%)
Frame = +1
Query: 373 INQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASETKEKDF 534
+N+WA++ T G I + ++ N ++F+G W F+ S TK+ +F
Sbjct: 16 LNKWASDHTNGLIIDLLPRGSVKSETVQVYGNALYFKGAWENKFDKSSTKDNEF 69
>At1g62170.1 68414.m07013 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
GI:9937311 from [Cucurbita maxima]; contains Pfam
profile PF00079: Serpin (serine protease inhibitor)
Length = 433
Score = 36.3 bits (80), Expect = 0.013
Identities = 19/69 (27%), Positives = 33/69 (47%), Gaps = 1/69 (1%)
Frame = +1
Query: 337 IDF-SDTKKAADIINQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNAS 513
+DF S ++ +N WA+ T G IK + ++ + ++F+G W ++ S
Sbjct: 190 VDFRSKAEEVRTEVNAWASSHTNGLIKDLLPRGSVTSLTDRVYGSALYFKGTWEEKYSKS 249
Query: 514 ETKEKDFML 540
TK K F L
Sbjct: 250 MTKCKPFYL 258
>At1g51330.1 68414.m05772 serpin-related / serine protease
inhibitor-related similar to serpin [Hordeum vulgare
subsp. vulgare] CAA64599.1 GI:1197577
Length = 193
Score = 35.5 bits (78), Expect = 0.023
Identities = 17/56 (30%), Positives = 25/56 (44%)
Frame = +1
Query: 373 INQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASETKEKDFML 540
+N WA T G IK+ + ++ N ++F+G W F S T K F L
Sbjct: 39 VNSWALRHTNGLIKNLLPPGSVTNQTIKIYGNALYFKGAWENKFGKSMTIHKPFHL 94
>At3g02280.1 68416.m00209 flavodoxin family protein low similarity
to SP|Q05001 NADPH-cytochrome P450 reductase (EC
1.6.2.4) {Catharanthus roseus}, similar to
NADPH-dependent FMN and FAD containing oxidoreductase
[Homo sapiens] GI:6694369; contains Pfam profiles
PF00258: flavodoxin, PF00667: FAD binding domain,
PF00175: Oxidoreductase NAD-binding domain
Length = 623
Score = 28.7 bits (61), Expect = 2.7
Identities = 22/80 (27%), Positives = 40/80 (50%), Gaps = 7/80 (8%)
Frame = +1
Query: 133 GSRAEIDKFLGNGD----YSGVANPY-ISLSKTFSEMNPDYFTMANKIYVGNKYTLDE-K 294
G+ I+K LG+ Y G +P+ +SL +T ++NP YF + + +D+ K
Sbjct: 123 GATTIIEKGLGDDQHPSGYEGTLDPWMLSLWRTLYQINPKYFPKGPDVKIPQDEVIDKPK 182
Query: 295 FTITV-RQYQSEVETIDFSD 351
+ I +Q + E + + SD
Sbjct: 183 YRILFHKQEKLEPKLLSDSD 202
>At1g70900.1 68414.m08181 expressed protein
Length = 244
Score = 28.7 bits (61), Expect = 2.7
Identities = 19/66 (28%), Positives = 32/66 (48%)
Frame = +1
Query: 64 KNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKTFSEMNPDYF 243
K +S +GV + LY + SR + K+L DY+ +A I L++ E NP +
Sbjct: 103 KVYANSLIGVGIASSLYHS-----SRGKFRKYLRWADYTMIATATICLTRALREENPKFL 157
Query: 244 TMANKI 261
A+ +
Sbjct: 158 MAASAL 163
>At1g23110.1 68414.m02889 hypothetical protein
Length = 261
Score = 28.7 bits (61), Expect = 2.7
Identities = 20/66 (30%), Positives = 32/66 (48%)
Frame = +1
Query: 64 KNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKTFSEMNPDYF 243
K +S +GV + LY A SR ++ K+L DY+ +A I LS+ NP +
Sbjct: 120 KVYANSLIGVGIASSLYHA-----SRGKLRKYLRWVDYTMIATTTICLSRALRNENPKFL 174
Query: 244 TMANKI 261
A+ +
Sbjct: 175 MAASAL 180
>At5g19010.1 68418.m02258 mitogen-activated protein kinase, putative
/ MAPK, putative (MPK16) mitogen-activated protein
kinase (MAPK)(AtMPK16), PMID:12119167; similar to
ATMPK9, Arabidopsis thaliana, EMBL:AB038694
Length = 567
Score = 27.9 bits (59), Expect = 4.7
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +1
Query: 127 GEGSRAEIDKFLGNGDYSGVANPY 198
GEGSR I++ +G G Y V + Y
Sbjct: 20 GEGSRYRIEEVIGKGSYGVVCSAY 43
>At5g14950.1 68418.m01754 glycosyl hydrolase family 38 protein
similar to alpha-mannosidase II SP:P27046 from [Mus
musculus]
Length = 1173
Score = 27.9 bits (59), Expect = 4.7
Identities = 10/29 (34%), Positives = 18/29 (62%)
Frame = -1
Query: 116 LYRSISIITPNGEDTTFLSFDDVYVSLRN 30
LYR+ +++ P G+D ++S D+ RN
Sbjct: 401 LYRTNTLLIPLGDDFRYISIDEAEAQFRN 429
>At4g01290.1 68417.m00170 expressed protein
Length = 991
Score = 27.9 bits (59), Expect = 4.7
Identities = 18/54 (33%), Positives = 26/54 (48%), Gaps = 4/54 (7%)
Frame = +2
Query: 251 PIKSTSEINILWMRNSRSLSVSTKAKLKLLT----SAIPKKQLTSLISGLMRRP 400
P+ + + + M R SV +A LL+ S+ PK Q T L+S RRP
Sbjct: 584 PVDQDTSVPSVKMTKQRKTSVDDQASQHLLSLLQRSSDPKSQDTQLLSATERRP 637
>At3g25140.1 68416.m03139 glycosyl transferase family 8 protein
contains Pfam profile: PF01501 glycosyl transferase
family 8
Length = 559
Score = 27.9 bits (59), Expect = 4.7
Identities = 17/77 (22%), Positives = 35/77 (45%)
Frame = +1
Query: 67 NVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKTFSEMNPDYFT 246
+VV+ + + + +++K +G+ E+ DY+ + + Y+ + K N F
Sbjct: 281 HVVTDKMNLGAMQVMFKLKEYKGAHVEVKAV---EDYTFLNSSYVPVLKQLESANLQKFY 337
Query: 247 MANKIYVGNKYTLDEKF 297
NK+ K T + KF
Sbjct: 338 FENKLENATKDTTNMKF 354
>At5g35940.1 68418.m04325 jacalin lectin family protein similar to
myrosinase-binding protein homolog [Arabidopsis
thaliana] GI:2997767; contains Pfam profile PF01419
jacalin-like lectin domain
Length = 444
Score = 27.5 bits (58), Expect = 6.2
Identities = 18/61 (29%), Positives = 26/61 (42%), Gaps = 1/61 (1%)
Frame = +1
Query: 61 DKNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKT-FSEMNPD 237
DKNV S LG + +L +G + + GDY G+ Y++ T M D
Sbjct: 131 DKNVYS--LGAYFIKVLATKSGMQGGQNTGKSYDYGGDYDGIRKVYVTYDGTSIRHMRVD 188
Query: 238 Y 240
Y
Sbjct: 189 Y 189
>At3g55340.1 68416.m06146 RNA recognition motif (RRM)-containing
protein low similarity to nucleolar phosphoprotein
(Nopp52), Tetrahymena thermophila, EMBL:TT51555;
contains InterPro entry IPR000504: RNA-binding region
RNP-1 (RNA recognition motif) (RRM)
Length = 597
Score = 27.5 bits (58), Expect = 6.2
Identities = 13/41 (31%), Positives = 19/41 (46%)
Frame = +1
Query: 151 DKFLGNGDYSGVANPYISLSKTFSEMNPDYFTMANKIYVGN 273
D++L Y P I KT S P+ N++Y+GN
Sbjct: 228 DRYLTIQQYVKTTTPSIPRRKTSSGFAPEMVDGYNRVYIGN 268
>At3g13170.1 68416.m01647 DNA topoisomerase VIA (SPO11-1) identical
to AtSPO11-1 [Arabidopsis thaliana] GI:13383478;
contains Pfam profile PF04406: Type IIB DNA
topoisomerase; identical to cDNA putative topoisomerase
VIA (SPO11 gene 1) GI:7270974
Length = 362
Score = 27.1 bits (57), Expect = 8.2
Identities = 13/27 (48%), Positives = 16/27 (59%)
Frame = +1
Query: 4 DAIDKTSLQFLKETYTSSKDKNVVSSP 84
+A+ SL FL E Y SK + VSSP
Sbjct: 336 EALSVHSLSFLSEVYIPSKIRREVSSP 362
>At2g23990.2 68415.m02866 plastocyanin-like domain-containing
protein
Length = 226
Score = 27.1 bits (57), Expect = 8.2
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = -1
Query: 173 SPLPKNLSISARLPSPAPALYRSISIITPNG 81
+P PK+ S S P+PAPA ++ ++ NG
Sbjct: 181 TPSPKS-STSTMAPAPAPAKSSAVGLVAGNG 210
>At2g23990.1 68415.m02865 plastocyanin-like domain-containing
protein
Length = 207
Score = 27.1 bits (57), Expect = 8.2
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = -1
Query: 173 SPLPKNLSISARLPSPAPALYRSISIITPNG 81
+P PK+ S S P+PAPA ++ ++ NG
Sbjct: 162 TPSPKS-STSTMAPAPAPAKSSAVGLVAGNG 191
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.315 0.131 0.375
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,762,138
Number of Sequences: 28952
Number of extensions: 245710
Number of successful extensions: 820
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 793
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 818
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1023490624
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.5 bits)
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