Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0005_F06
(653 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g26390.1 68415.m03167 serpin, putative / serine protease inhi... 48 5e-06
At3g45220.1 68416.m04880 serpin, putative / serine protease inhi... 45 5e-05
At1g64030.1 68414.m07252 serpin family protein / serine protease... 43 2e-04
At1g47710.1 68414.m05302 serpin, putative / serine protease inhi... 40 0.001
At3g55340.1 68416.m06146 RNA recognition motif (RRM)-containing ... 31 0.67
At3g25140.1 68416.m03139 glycosyl transferase family 8 protein c... 29 3.6
At4g36160.1 68417.m05146 no apical meristem (NAM) family protein... 28 4.7
At1g76740.1 68414.m08931 expressed protein weak similarity to fi... 28 4.7
At5g39785.2 68418.m04819 expressed protein 28 6.2
At5g39785.1 68418.m04818 expressed protein 28 6.2
At5g28970.1 68418.m03584 Ulp1 protease family protein contains P... 28 6.2
At4g04010.1 68417.m00571 Ulp1 protease family protein contains P... 28 6.2
At2g20580.1 68415.m02404 26S proteasome regulatory subunit S2 (R... 28 6.2
At1g23110.1 68414.m02889 hypothetical protein 27 8.2
>At2g26390.1 68415.m03167 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 389
Score = 48.0 bits (109), Expect = 5e-06
Identities = 40/175 (22%), Positives = 78/175 (44%), Gaps = 8/175 (4%)
Frame = +3
Query: 117 DAIDKTSMKLLKEAYTSGKDKNVVSSPLGVMMLMLLYKSGAGEGSRVEIDKFLGDVDYSE 296
+ + + + K+++ +G NVV SP+ + +L+ L +G+ ++ EI FL
Sbjct: 12 NVVARLAKKVIETDVANGS--NVVFSPMSINVLLSLIAAGSNPVTKEEILSFLMSPSTDH 69
Query: 297 ATNPYISLSKTFSEMNPDFFTMANKIYVGNKYTLDEKFTS-SSRQYQSEVETIDFSDTK- 470
++ +E + + A+ +++ L F Y++ +DF+ TK
Sbjct: 70 LNAVLAKIADGGTERSDLCLSTAHGVWIDKSSYLKPSFKELLENSYKASCSQVDFA-TKP 128
Query: 471 -KAADIINQWANEKTRGHIKSPISDDTIDP-----NAAAALFNVIFFQGHLARAF 617
+ D +N WA+ T G IK +S D D N+ L N ++F+ +R F
Sbjct: 129 VEVIDEVNIWADVHTNGLIKQILSRDCTDTIKEIRNSTLILANAVYFKAAWSRKF 183
>At3g45220.1 68416.m04880 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 393
Score = 44.8 bits (101), Expect = 5e-05
Identities = 41/161 (25%), Positives = 71/161 (44%), Gaps = 9/161 (5%)
Frame = +3
Query: 162 TSGKDKNVVSSPLGVMMLMLLYKSGAGEGSRVEIDKF--LGDVDYSEAT-NPYISLSKTF 332
T N+V SP+ + +L+ L +G+ ++ +I F L DY A +S++
Sbjct: 24 TVANGSNLVFSPMSINVLLCLIAAGSNCVTKEQILSFIMLPSSDYLNAVLAKTVSVALND 83
Query: 333 SEMNPDF-FTMANKIYVGNKYTLDEKFTSS-SRQYQSEVETIDFSDTKKAADI--INQWA 500
D + A +++ + F Y + +DF+ TK A I +N WA
Sbjct: 84 GMERSDLHLSTAYGVWIDKSLSFKPSFKDLLENSYNATCNQVDFA-TKPAEVINEVNAWA 142
Query: 501 NEKTRGHIKSPISDDTIDP--NAAAALFNVIFFQGHLARAF 617
T G IK +SDD+I + L N ++F+G ++ F
Sbjct: 143 EVHTNGLIKEILSDDSIKTIRESMLILANAVYFKGAWSKKF 183
>At1g64030.1 68414.m07252 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
[Cucurbita maxima] GI:9937311, serpin [Triticum
aestivum] GI:871551; contains Pfam profile PF00079:
Serpin (serine protease inhibitor)
Length = 385
Score = 43.2 bits (97), Expect = 2e-04
Identities = 37/159 (23%), Positives = 62/159 (38%), Gaps = 8/159 (5%)
Frame = +3
Query: 171 KDKNVVSSPLGVMMLMLLYKSGAGEGSRV--EIDKFLGDVDYSEATNPYISLSKTF---- 332
KD NV+ SP + + ++ +G G G V +I FL E + L+
Sbjct: 27 KDSNVIFSPASINSAITMHAAGPG-GDLVSGQILSFLRSSSIDELKTVFRELASVVYADR 85
Query: 333 SEMNPDFFTMANKIYVGNKYTLDEKFTSSSRQYQSEVET-IDF-SDTKKAADIINQWANE 506
S T AN +++ D KF + V +DF S+ ++ +N W
Sbjct: 86 SATGGPKITAANGLWIDKSLPTDPKFKDLFENFFKAVYVPVDFRSEAEEVRKEVNSWVEH 145
Query: 507 KTRGHIKSPISDDTIDPNAAAALFNVIFFQGHLARAFQR 623
T IK + D ++ N + F+G R F++
Sbjct: 146 HTNNLIKDLLPDGSVTSLTNKIYANALSFKGAWKRPFEK 184
>At1g47710.1 68414.m05302 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 391
Score = 40.3 bits (90), Expect = 0.001
Identities = 35/168 (20%), Positives = 76/168 (45%), Gaps = 10/168 (5%)
Frame = +3
Query: 126 DKTSMKLLKEAYTS-GKDKNVVSSPLGVMMLMLLYKSGAGEGSRVEIDKFLGDVDYSEAT 302
++ SM L K T+ ++ NV+ SP + +++ + +G+ ++ +I FL ++
Sbjct: 11 NQVSMNLAKHVITTVSQNSNVIFSPASINVVLSIIAAGSAGATKDQILSFL-KFSSTDQL 69
Query: 303 NPYIS--LSKTFSEMNPDF---FTMANKIYVGNKYTLDEKFTSSSRQ-YQSEVETIDFSD 464
N + S +S ++ + + ++AN ++ + F Y++ DF
Sbjct: 70 NSFSSEIVSAVLADGSANGGPKLSVANGAWIDKSLSFKPSFKQLLEDSYKAASNQADFQS 129
Query: 465 TKKAADII---NQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQG 599
KA ++I N WA ++T G I + + + D N ++F+G
Sbjct: 130 --KAVEVIAEVNSWAEKETNGLITEVLPEGSADSMTKLIFANALYFKG 175
>At3g55340.1 68416.m06146 RNA recognition motif (RRM)-containing
protein low similarity to nucleolar phosphoprotein
(Nopp52), Tetrahymena thermophila, EMBL:TT51555;
contains InterPro entry IPR000504: RNA-binding region
RNP-1 (RNA recognition motif) (RRM)
Length = 597
Score = 31.1 bits (67), Expect = 0.67
Identities = 14/41 (34%), Positives = 21/41 (51%)
Frame = +3
Query: 264 DKFLGDVDYSEATNPYISLSKTFSEMNPDFFTMANKIYVGN 386
D++L Y + T P I KT S P+ N++Y+GN
Sbjct: 228 DRYLTIQQYVKTTTPSIPRRKTSSGFAPEMVDGYNRVYIGN 268
>At3g25140.1 68416.m03139 glycosyl transferase family 8 protein
contains Pfam profile: PF01501 glycosyl transferase
family 8
Length = 559
Score = 28.7 bits (61), Expect = 3.6
Identities = 18/77 (23%), Positives = 35/77 (45%)
Frame = +3
Query: 180 NVVSSPLGVMMLMLLYKSGAGEGSRVEIDKFLGDVDYSEATNPYISLSKTFSEMNPDFFT 359
+VV+ + + + +++K +G+ VE+ DY+ + Y+ + K N F
Sbjct: 281 HVVTDKMNLGAMQVMFKLKEYKGAHVEVKAV---EDYTFLNSSYVPVLKQLESANLQKFY 337
Query: 360 MANKIYVGNKYTLDEKF 410
NK+ K T + KF
Sbjct: 338 FENKLENATKDTTNMKF 354
>At4g36160.1 68417.m05146 no apical meristem (NAM) family protein
similar to NAC2 (GI:6456751) [Arabidopsis thaliana];
contains Pfam PF02365 : No apical meristem (NAM) protein
Length = 377
Score = 28.3 bits (60), Expect = 4.7
Identities = 17/64 (26%), Positives = 32/64 (50%), Gaps = 2/64 (3%)
Frame = +3
Query: 75 FYFFDHEYNRTALGDAIDKTSMKLLKEAYTSGKDKNVV--SSPLGVMMLMLLYKSGAGEG 248
+YFF H+ + G ++ +M +A +G+DK V S +G+ ++ YK A G
Sbjct: 69 WYFFSHKDKKYPTGTRTNRATMAGFWKA--TGRDKAVYDKSKLIGMRKTLVFYKGRAPNG 126
Query: 249 SRVE 260
+ +
Sbjct: 127 QKTD 130
>At1g76740.1 68414.m08931 expressed protein weak similarity to
fimbriae-associated protein Fap1 (GI:3929312)
[Streptococcus parasanguinis]; weak similarity to 1MDa_1
protein (GI:24620455) [Caenorhabditis elegans]
Length = 1532
Score = 28.3 bits (60), Expect = 4.7
Identities = 26/96 (27%), Positives = 39/96 (40%), Gaps = 1/96 (1%)
Frame = +3
Query: 216 MLLYKSGAGEGSRVEIDKFLGDVDYSEATNPY-ISLSKTFSEMNPDFFTMANKIYVGNKY 392
+LL SG G E G D EA P I+ K S + PD T+ N + N+
Sbjct: 1383 LLLNSSGGGSNVSGETSILAGTADEDEAAGPIAIAFVKAMS-LTPDELTVKNHM---NEK 1438
Query: 393 TLDEKFTSSSRQYQSEVETIDFSDTKKAADIINQWA 500
DE+ + E + S+ +K I++ A
Sbjct: 1439 AADEEMITRETTPIPEETINESSENRKEYSSIDESA 1474
>At5g39785.2 68418.m04819 expressed protein
Length = 607
Score = 27.9 bits (59), Expect = 6.2
Identities = 18/81 (22%), Positives = 35/81 (43%), Gaps = 5/81 (6%)
Frame = +3
Query: 267 KFLGDVDYSEATNPYISLSKTFSEMNPDFFT-----MANKIYVGNKYTLDEKFTSSSRQY 431
+FL + D+ E+ + ++ S+TF+ + D F + G D + S +
Sbjct: 135 RFLTEEDFLESDSDFVDSSQTFTSNDEDGFLSDSDFAETSLKKGQNRKSDNSGSGSDSEE 194
Query: 432 QSEVETIDFSDTKKAADIINQ 494
+ E +T F + D+I Q
Sbjct: 195 EEEEDTNGFESLWEHQDLIEQ 215
>At5g39785.1 68418.m04818 expressed protein
Length = 606
Score = 27.9 bits (59), Expect = 6.2
Identities = 18/81 (22%), Positives = 35/81 (43%), Gaps = 5/81 (6%)
Frame = +3
Query: 267 KFLGDVDYSEATNPYISLSKTFSEMNPDFFT-----MANKIYVGNKYTLDEKFTSSSRQY 431
+FL + D+ E+ + ++ S+TF+ + D F + G D + S +
Sbjct: 135 RFLTEEDFLESDSDFVDSSQTFTSNDEDGFLSDSDFAETSLKKGQNRKSDNSGSGSDSEE 194
Query: 432 QSEVETIDFSDTKKAADIINQ 494
+ E +T F + D+I Q
Sbjct: 195 EEEEDTNGFESLWEHQDLIEQ 215
>At5g28970.1 68418.m03584 Ulp1 protease family protein contains Pfam
profile PF02902: Ulp1 protease family, C-terminal
catalytic domain
Length = 1172
Score = 27.9 bits (59), Expect = 6.2
Identities = 28/101 (27%), Positives = 43/101 (42%), Gaps = 13/101 (12%)
Frame = +3
Query: 315 SLSKTFSEMNPDFFTMANKIYVGNKYTLDEKFTSSSRQYQSEVETI------DFSDTKKA 476
SL +T E+N D +M +Y+ N+ TL+ +S S E I D +T A
Sbjct: 625 SLLETQPEVNIDSDSMKLHVYINNQVTLEMSASSHSVSEAQPEEKIGNDPMEDTMETPPA 684
Query: 477 ADIINQWA-------NEKTRGHIKSPISDDTIDPNAAAALF 578
A ++ +A +T I S + D DP A +F
Sbjct: 685 AHSLSGFAMFSKKKVTMETAAAIHSGVCKDNTDPMKAMVVF 725
>At4g04010.1 68417.m00571 Ulp1 protease family protein contains Pfam
profile PF02902: Ulp1 protease family, C-terminal
catalytic domain; similar to At1g32840, At2g06430,
At2g15140, At2g04980, At2g14130, At3g44500, At2g15190,
At3g47260, At5g34900, At3g29210, At2g02210, At3g32900
Length = 836
Score = 27.9 bits (59), Expect = 6.2
Identities = 15/48 (31%), Positives = 26/48 (54%), Gaps = 5/48 (10%)
Frame = +3
Query: 312 ISLSKTFSEMNPDFFTMANKIYVGNKY--TLDE---KFTSSSRQYQSE 440
+ L KT E NP+ F + ++VG+ + +DE +F + +QSE
Sbjct: 646 LELMKTRKESNPELFKNKSVVFVGSSFLNVIDESDMEFLDNKEGFQSE 693
>At2g20580.1 68415.m02404 26S proteasome regulatory subunit S2
(RPN1) contains an APC-complex (cyclosome) and
proteasome component repeat ( PS50248)
Length = 891
Score = 27.9 bits (59), Expect = 6.2
Identities = 12/44 (27%), Positives = 22/44 (50%)
Frame = -1
Query: 503 ISPLINDVSCFFGIAEVNSFNLALVLTGRAREFLIQSIFISDVD 372
+SP++ND + S +L ++ G E + QSI + +D
Sbjct: 506 LSPILNDAKAPLDVIAFASLSLGMIYVGSCNEEVAQSIIFALMD 549
>At1g23110.1 68414.m02889 hypothetical protein
Length = 261
Score = 27.5 bits (58), Expect = 8.2
Identities = 20/66 (30%), Positives = 31/66 (46%)
Frame = +3
Query: 177 KNVVSSPLGVMMLMLLYKSGAGEGSRVEIDKFLGDVDYSEATNPYISLSKTFSEMNPDFF 356
K +S +GV + LY + SR ++ K+L VDY+ I LS+ NP F
Sbjct: 120 KVYANSLIGVGIASSLYHA-----SRGKLRKYLRWVDYTMIATTTICLSRALRNENPKFL 174
Query: 357 TMANKI 374
A+ +
Sbjct: 175 MAASAL 180
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,922,608
Number of Sequences: 28952
Number of extensions: 287103
Number of successful extensions: 850
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 838
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 850
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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