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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0005_E24
         (652 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_24829| Best HMM Match : Gelsolin (HMM E-Value=5.8e-35)             143   1e-34
SB_24828| Best HMM Match : Peptidase_A17 (HMM E-Value=1.7e-23)        121   6e-28
SB_54820| Best HMM Match : No HMM Matches (HMM E-Value=.)              67   1e-11
SB_14143| Best HMM Match : Gelsolin (HMM E-Value=1.1e-07)              56   2e-08
SB_14633| Best HMM Match : LRR_1 (HMM E-Value=3.4e-12)                 55   4e-08
SB_7860| Best HMM Match : Gelsolin (HMM E-Value=0.0032)                55   6e-08
SB_20689| Best HMM Match : Gelsolin (HMM E-Value=0.00029)              50   2e-06
SB_43866| Best HMM Match : Gelsolin (HMM E-Value=0.092)                48   9e-06
SB_14141| Best HMM Match : HH_signal (HMM E-Value=0)                   46   2e-05
SB_38607| Best HMM Match : Gelsolin (HMM E-Value=0.0024)               38   0.005
SB_54818| Best HMM Match : No HMM Matches (HMM E-Value=.)              33   0.15 
SB_32906| Best HMM Match : No HMM Matches (HMM E-Value=.)              31   1.1  
SB_3684| Best HMM Match : fn3 (HMM E-Value=8e-11)                      30   1.4  
SB_56058| Best HMM Match : Phasin (HMM E-Value=2.7)                    30   1.4  
SB_49838| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   1.9  
SB_7858| Best HMM Match : Gelsolin (HMM E-Value=0.35)                  29   3.3  
SB_18647| Best HMM Match : SH2 (HMM E-Value=7.3e-16)                   28   7.6  

>SB_24829| Best HMM Match : Gelsolin (HMM E-Value=5.8e-35)
          Length = 1078

 Score =  143 bits (347), Expect = 1e-34
 Identities = 84/219 (38%), Positives = 120/219 (54%), Gaps = 3/219 (1%)
 Frame = +2

Query: 2    RTKARVHPAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDK-RNNL 178
            R  A    A+ NAG + G++IWRI+ F+      +D+G FY GDSYIIL T  +   + L
Sbjct: 460  REAAATEIAWKNAGTREGLQIWRIEKFKVKVWSREDYGSFYDGDSYIILNTYKESGEDEL 519

Query: 179  SWDIHYWIGSETSQDEAGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYL 358
             +D+H+WIG +++QDE G AA  TV LD       +QHRE  G ES  F  YF S L  L
Sbjct: 520  KYDVHFWIGKDSTQDEYGTAAYKTVELDIHLNDKPIQHREVQGFESKLFKSYFKS-LTIL 578

Query: 359  DGGNPSGFNHVVTNPGAEKRMFQVKGK--RNVRVRQVDPLISSMNKGDCFILDINNDIYV 532
             GG  SGF HV      + R+ +V+G    N  V +V    SS+   D FILD   ++Y+
Sbjct: 579  KGGVDSGFRHVKPQE-YKPRLLRVRGTTVSNCVVEEVLLARSSLCSEDVFILDKGLNLYL 637

Query: 533  YVGERTKNVEKLKAISMANQIRDQDHNGRGRVEIIDPYS 649
            +VG +    EK + +    +I+  + NG+ + E+ D  S
Sbjct: 638  WVGVKCDKDEKFRGMQEILKIK-SERNGKPKSEVNDESS 675



 Score = 74.1 bits (174), Expect = 9e-14
 Identities = 52/179 (29%), Positives = 89/179 (49%), Gaps = 6/179 (3%)
 Frame = +2

Query: 92   AVQSKDFGKFYKGDSYIILKTTSD-KRNNLSWDIHYWIGSETSQDEAGAAAILTVGLDDK 268
            +V   D+GKFY GDSYIIL T  D + + L +D+H+WIG +++QDE G AA  TV LD  
Sbjct: 879  SVLRDDYGKFYDGDSYIILNTYKDPEEDELKYDVHFWIGKDSTQDEYGTAAYKTVELDIY 938

Query: 269  FGGAAVQH----REAMGHESNHFLQYFTSALQYLDGGNPSGFNHVVTNPGAEKRMFQVKG 436
                 V+     R+++  +    L    +  Q  D  +       V++   + ++ ++K 
Sbjct: 939  PKSVKVEEVPFVRKSLNSDDVFILDKGKTIYQKGDYDDFEPVLLRVSDASGQMKLTEMK- 997

Query: 437  KRNVRVRQVDPLISSMNKGDCFILDINNDIYVYVGERTKNVEKLKAISM-ANQIRDQDH 610
            K   R+ + D      ++ D F+ D  N ++VY G +    E+  A+ +  N +   +H
Sbjct: 998  KGKGRITRND-----FDEKDVFLFDTGNVLFVYSGNKASIDERRLALQIGTNYLNGTNH 1051


>SB_24828| Best HMM Match : Peptidase_A17 (HMM E-Value=1.7e-23)
          Length = 1531

 Score =  121 bits (291), Expect = 6e-28
 Identities = 64/150 (42%), Positives = 88/150 (58%), Gaps = 2/150 (1%)
 Frame = +2

Query: 2   RTKARVHPAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNN-- 175
           ++ A    A+ NAG + G++IWRI  F+      + +G+FY GDSYIIL T  +K +   
Sbjct: 505 KSAAEGEVAWKNAGEKVGLQIWRINKFKVEEWPKEKYGQFYAGDSYIILWTYEEKEDTEK 564

Query: 176 LSWDIHYWIGSETSQDEAGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQY 355
           L +D+H+WIG  ++QDE G AA  TV LD       VQHRE M HES+ F  YF S + Y
Sbjct: 565 LCYDLHFWIGRGSTQDEYGTAAYKTVELDTYLNDVPVQHREIMNHESDMFKTYFKS-ITY 623

Query: 356 LDGGNPSGFNHVVTNPGAEKRMFQVKGKRN 445
           L GG  +GFN V      + R+ +V G +N
Sbjct: 624 LKGGAETGFNQVKPKE-YKPRILKVSGDKN 652


>SB_54820| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 313

 Score = 67.3 bits (157), Expect = 1e-11
 Identities = 60/203 (29%), Positives = 90/203 (44%), Gaps = 16/203 (7%)
 Frame = +2

Query: 17  VHPAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNNLSWD--- 187
           V  AF  AG++ G+EIWRI+  + VA   K +G FY GDSYI L      ++    D   
Sbjct: 3   VDDAFVQAGQKPGLEIWRIEKLKVVAQDPKTYGTFYSGDSYICLSVELHYKSLNKGDVFI 62

Query: 188 ------IHYWIGSETSQDEAGAAAILTVGL-DDKFGGAAVQH----REAMGHESNHFLQY 334
                 I+ W GS+ S+ E      +   + D++ GG A  H     +  G ES  F   
Sbjct: 63  LDDGLNIYCWNGSQCSRVERMKGIDVAKRIRDEERGGRAQVHIIDECKDKGLESKFFDAL 122

Query: 335 FTSALQYLDGGNPSGFNHVVTNPGAEKRMFQVKGKRNVRVRQVDPL-ISSMNKGDCFILD 511
            +      D G           P     M    G+  +R  +  PL  S+++  DCFILD
Sbjct: 123 GSRGEIAEDLGTTRSLRKPARAPSL-STMSDASGELEMREIEAKPLKKSNLDTNDCFILD 181

Query: 512 I-NNDIYVYVGERTKNVEKLKAI 577
             ++ ++V+VG+     EK  A+
Sbjct: 182 CGSSGVFVWVGKGCTKNEKSAAM 204



 Score = 58.4 bits (135), Expect = 5e-09
 Identities = 26/54 (48%), Positives = 37/54 (68%)
 Frame = +2

Query: 479 SMNKGDCFILDINNDIYVYVGERTKNVEKLKAISMANQIRDQDHNGRGRVEIID 640
           S+NKGD FILD   +IY + G +   VE++K I +A +IRD++  GR +V IID
Sbjct: 54  SLNKGDVFILDDGLNIYCWNGSQCSRVERMKGIDVAKRIRDEERGGRAQVHIID 107


>SB_14143| Best HMM Match : Gelsolin (HMM E-Value=1.1e-07)
          Length = 546

 Score = 56.4 bits (130), Expect = 2e-08
 Identities = 32/84 (38%), Positives = 47/84 (55%), Gaps = 1/84 (1%)
 Frame = +2

Query: 41  GRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNNLSWDIHYWIGSETSQ 220
           GR   V  +R+ +   V   +   G F+ GDSY+++ T   +R      I++W G+++  
Sbjct: 155 GRSKEVGFYRVTDGGKVQCNTAAKGIFFSGDSYLVVYTYRTQRGQKKSIIYFWKGNDSRV 214

Query: 221 DEAGAAAILTVGLD-DKFGGAAVQ 289
            E GAAA LTV LD + FGG AVQ
Sbjct: 215 FEKGAAAKLTVDLDNNNFGGDAVQ 238



 Score = 27.9 bits (59), Expect = 7.6
 Identities = 21/78 (26%), Positives = 36/78 (46%), Gaps = 3/78 (3%)
 Frame = +2

Query: 338 TSALQYLDGGNPSGFNHVVTNPGA-EKRMFQVKGKR-NVRVRQVDPL-ISSMNKGDCFIL 508
           T   + L G  P   N ++ +  A E R+F    KR ++ V ++       +   D   +
Sbjct: 302 TKFWEILGGKKPYANNILLQSSEAPEPRLFHCYNKRGSLEVEEIVNFGQDDLVDDDVMFV 361

Query: 509 DINNDIYVYVGERTKNVE 562
           D+ + IY++VGE  K  E
Sbjct: 362 DLGDHIYMWVGEEAKKSE 379


>SB_14633| Best HMM Match : LRR_1 (HMM E-Value=3.4e-12)
          Length = 446

 Score = 55.2 bits (127), Expect = 4e-08
 Identities = 41/134 (30%), Positives = 62/134 (46%)
 Frame = +2

Query: 146 LKTTSDKRNNLSWDIHYWIGSETSQDEAGAAAILTVGLDDKFGGAAVQHREAMGHESNHF 325
           LKT  D+ + L W I+YWIG E S                         RE  G ES  F
Sbjct: 260 LKTELDETDQLFWQIYYWIGKEAS-------------------------REEQGDESEEF 294

Query: 326 LQYFTSALQYLDGGNPSGFNHVVTNPGAEKRMFQVKGKRNVRVRQVDPLISSMNKGDCFI 505
            + F + + Y++GG  SGF + V +     R+F++   + V +  V P +SS++    FI
Sbjct: 295 HELFDNDIAYIEGGTASGF-YSVEDQIYITRLFRLLKDKRVLLEPVLPDVSSLDPTFTFI 353

Query: 506 LDINNDIYVYVGER 547
           LD    IY++ G +
Sbjct: 354 LDAGLKIYIWSGAK 367


>SB_7860| Best HMM Match : Gelsolin (HMM E-Value=0.0032)
          Length = 675

 Score = 54.8 bits (126), Expect = 6e-08
 Identities = 22/51 (43%), Positives = 32/51 (62%)
 Frame = +2

Query: 2   RTKARVHPAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKT 154
           +  A   PA+  AG++ GV++WRI  F+      +D+G FY GDSYI+L T
Sbjct: 352 KESAEGEPAWEGAGKEVGVQVWRIVKFKVTHWPKQDYGHFYNGDSYIVLNT 402



 Score = 28.3 bits (60), Expect = 5.7
 Identities = 12/39 (30%), Positives = 23/39 (58%)
 Frame = +2

Query: 476 SSMNKGDCFILDINNDIYVYVGERTKNVEKLKAISMANQ 592
           S +N  D FI+D  +  YV+ G+ +   E+ +A+  A++
Sbjct: 594 SQLNTNDVFIMDSGDHCYVWSGKGSSVDERRRAMEFAHR 632


>SB_20689| Best HMM Match : Gelsolin (HMM E-Value=0.00029)
          Length = 1866

 Score = 50.0 bits (114), Expect = 2e-06
 Identities = 17/54 (31%), Positives = 35/54 (64%)
 Frame = +2

Query: 434  GKRNVRVRQVDPLISSMNKGDCFILDINNDIYVYVGERTKNVEKLKAISMANQI 595
            G+RN++ R ++P   S N GDC++L   ++++ +VG+    +E+ K   +A++I
Sbjct: 1457 GRRNIQTRLIEPCQKSFNSGDCYVLVTKDELFAWVGKHANAIERAKVTEIASRI 1510



 Score = 38.3 bits (85), Expect = 0.005
 Identities = 22/96 (22%), Positives = 46/96 (47%), Gaps = 16/96 (16%)
 Frame = +2

Query: 35   NAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIIL--------KTTSDKRNNLSWDI 190
            N     GV +W I  +    + +++ G F+ G+ Y+I         +  +D+++     +
Sbjct: 1769 NLCNTVGVTVWHINEYRHYELPNENHGHFHSGEGYVIRWAYFVMTDRIATDRKSRCRSTV 1828

Query: 191  --------HYWIGSETSQDEAGAAAILTVGLDDKFG 274
                     +W G++ + +E GAAA++ V LD++ G
Sbjct: 1829 AGRVRTAYFFWQGNDCTVNEKGAAAVMAVELDEERG 1864



 Score = 34.3 bits (75), Expect = 0.087
 Identities = 13/60 (21%), Positives = 32/60 (53%)
 Frame = +2

Query: 467  PLISSMNKGDCFILDINNDIYVYVGERTKNVEKLKAISMANQIRDQDHNGRGRVEIIDPY 646
            P IS ++  +  + D  +++YV+ G+++ + ++    ++  Q+ +    G G  + I PY
Sbjct: 1598 PQISLLDTKEVLVFDFGSELYVWNGQQSLSGQRKTVFALGKQLYESSFKGHGPYDPIYPY 1657


>SB_43866| Best HMM Match : Gelsolin (HMM E-Value=0.092)
          Length = 341

 Score = 47.6 bits (108), Expect = 9e-06
 Identities = 23/53 (43%), Positives = 32/53 (60%)
 Frame = +2

Query: 185 DIHYWIGSETSQDEAGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTS 343
           ++H+W+GSE S +E   AA   V L  K  G  VQ+REA G+E+  F + F S
Sbjct: 22  NLHFWVGSECSAEEYCTAAYKAVELFVKLEGKPVQYREAEGYETEEFRKCFQS 74


>SB_14141| Best HMM Match : HH_signal (HMM E-Value=0)
          Length = 754

 Score = 46.4 bits (105), Expect = 2e-05
 Identities = 30/85 (35%), Positives = 41/85 (48%), Gaps = 2/85 (2%)
 Frame = +2

Query: 77  NFEPVAVQSKDFGK-FYKGDSYIILKTTSDKRNNLSWDIHYWIGSETSQDEAGAAAILTV 253
           N + V +++K   + FY GDSY+IL    DK N     +H   G   S DE   AA   +
Sbjct: 662 NMKLVKMENKVIKEGFYDGDSYVILDYRKDKTNKKQPVLHILHGKNASTDELFFAATKAI 721

Query: 254 GLDDK-FGGAAVQHREAMGHESNHF 325
            +DD+ FGG A Q  +    E   F
Sbjct: 722 AIDDEYFGGKAKQTVQVCCREKVQF 746


>SB_38607| Best HMM Match : Gelsolin (HMM E-Value=0.0024)
          Length = 693

 Score = 38.3 bits (85), Expect = 0.005
 Identities = 22/96 (22%), Positives = 46/96 (47%), Gaps = 16/96 (16%)
 Frame = +2

Query: 35  NAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIIL--------KTTSDKRNNLSWDI 190
           N     GV +W I  +    + +++ G F+ G+ Y+I         +  +D+++     +
Sbjct: 214 NLCNTVGVTVWHINEYRHYELPNENHGHFHSGEGYVIRWAYFVMTDRIATDRKSRCRSTV 273

Query: 191 --------HYWIGSETSQDEAGAAAILTVGLDDKFG 274
                    +W G++ + +E GAAA++ V LD++ G
Sbjct: 274 AGRVRTAYFFWQGNDCTVNEKGAAAVMAVELDEERG 309



 Score = 34.3 bits (75), Expect = 0.087
 Identities = 13/60 (21%), Positives = 32/60 (53%)
 Frame = +2

Query: 467 PLISSMNKGDCFILDINNDIYVYVGERTKNVEKLKAISMANQIRDQDHNGRGRVEIIDPY 646
           P IS ++  +  + D  +++YV+ G+++ + ++    ++  Q+ +    G G  + I PY
Sbjct: 43  PQISLLDTKEVLVFDFGSELYVWNGQQSLSGQRKTVFALGKQLYESSFKGHGPYDPIYPY 102


>SB_54818| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 251

 Score = 33.5 bits (73), Expect = 0.15
 Identities = 21/62 (33%), Positives = 28/62 (45%), Gaps = 3/62 (4%)
 Frame = +2

Query: 287 QHREAMGHESNHFLQYFTSALQYLDGGNPSGFN---HVVTNPGAEKRMFQVKGKRNVRVR 457
           Q R     E  HFL+ F   L  L+GG  +GF       T     KRMF VKG  ++  +
Sbjct: 38  QVRVVQYKEPEHFLRIFRGRLIILEGGKGAGFRAGCEEDTYDHEGKRMFHVKGTTDLNAK 97

Query: 458 QV 463
            +
Sbjct: 98  AI 99


>SB_32906| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 770

 Score = 30.7 bits (66), Expect = 1.1
 Identities = 18/59 (30%), Positives = 31/59 (52%), Gaps = 2/59 (3%)
 Frame = +2

Query: 194 YWIGSETSQDEAGAAAILTVGLDDKFG-GAAVQHREAMGHESNHFLQY-FTSALQYLDG 364
           +W G +++ +E GAAA++TV +D + G     +H   +  + + F  Y   S   Y DG
Sbjct: 197 FWQGHDSTVNEKGAAALMTVEIDSEKGPQITFKHTVTVRFDIDTFFIYRECSCFFYCDG 255


>SB_3684| Best HMM Match : fn3 (HMM E-Value=8e-11)
          Length = 862

 Score = 30.3 bits (65), Expect = 1.4
 Identities = 13/41 (31%), Positives = 20/41 (48%), Gaps = 2/41 (4%)
 Frame = -2

Query: 390 TWLKPDGLPPSKYCSADVKYCRK--WLLSWPIASRCCTAAP 274
           TW KPDG+P        V +C +  W ++ PI +    + P
Sbjct: 388 TWQKPDGIPDKYIPGVSVSWCYQCDWEVTPPIVTETSVSVP 428


>SB_56058| Best HMM Match : Phasin (HMM E-Value=2.7)
          Length = 314

 Score = 30.3 bits (65), Expect = 1.4
 Identities = 13/48 (27%), Positives = 26/48 (54%)
 Frame = +3

Query: 213 LVRMKRERLPS*LSASMTSSVERRYNTARLWATRVTTSYSTSHLHYNT 356
           L+R+ +E L   LS  + + ++  +N   LW+   ++S  ++H H  T
Sbjct: 122 LIRLGKEELRR-LSIQLVNKLDTTWNNCLLWSIPTSSSLDSAHAHLKT 168


>SB_49838| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 65

 Score = 29.9 bits (64), Expect = 1.9
 Identities = 11/27 (40%), Positives = 20/27 (74%)
 Frame = +2

Query: 194 YWIGSETSQDEAGAAAILTVGLDDKFG 274
           +W G +++ +E GAAA++TV +D + G
Sbjct: 37  FWQGHDSTVNEKGAAALMTVEIDSEKG 63


>SB_7858| Best HMM Match : Gelsolin (HMM E-Value=0.35)
          Length = 103

 Score = 29.1 bits (62), Expect = 3.3
 Identities = 16/53 (30%), Positives = 25/53 (47%)
 Frame = +2

Query: 482 MNKGDCFILDINNDIYVYVGERTKNVEKLKAISMANQIRDQDHNGRGRVEIID 640
           M   D FI+D+   IY +VG      EK KA      I+D+   G  +  +++
Sbjct: 1   MCNSDVFIVDLGLVIYQWVGCDASPFEKNKAAQYIQVIKDERSGGDIKTHLLE 53


>SB_18647| Best HMM Match : SH2 (HMM E-Value=7.3e-16)
          Length = 720

 Score = 27.9 bits (59), Expect = 7.6
 Identities = 14/41 (34%), Positives = 22/41 (53%)
 Frame = +2

Query: 383 NHVVTNPGAEKRMFQVKGKRNVRVRQVDPLISSMNKGDCFI 505
           NHV    G E  +  VKG    ++R+ D L+  + KG+ F+
Sbjct: 632 NHVKLGYGGEV-LEAVKGNNFYKLRKADQLMHQITKGNAFV 671


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,342,038
Number of Sequences: 59808
Number of extensions: 469826
Number of successful extensions: 1383
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 1239
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1374
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1657237625
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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