BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0005_E22 (593 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein... 67 2e-12 SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 56 4e-09 SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ... 42 5e-05 SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma... 41 2e-04 SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 27 2.1 SPCC794.06 |||TDT malic acid transporter|Schizosaccharomyces pom... 27 2.7 SPAC26A3.12c |dhp1||5'-3' exoribonuclease Dhp1 |Schizosaccharomy... 27 2.7 SPCC1672.12c |||DUF410 family protein|Schizosaccharomyces pombe|... 26 3.6 SPAP27G11.05c |vps41||vacuolar protein sorting-associated protei... 26 4.8 SPAC25H1.07 |||DUF1620 family protein|Schizosaccharomyces pombe|... 26 4.8 SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces... 25 6.3 SPAC1486.03c |||RNA-binding splicing factor|Schizosaccharomyces ... 25 8.3 SPBC13E7.05 |gpi14||pig-M|Schizosaccharomyces pombe|chr 2|||Manual 25 8.3 SPCC16C4.14c |sfc4||transcription factor TFIIIC complex subunit ... 25 8.3 SPAC1610.04 |mug99||meiotically upregulated gene Mug99|Schizosac... 25 8.3 >SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein Stg1|Schizosaccharomyces pombe|chr 1|||Manual Length = 174 Score = 66.9 bits (156), Expect = 2e-12 Identities = 48/137 (35%), Positives = 72/137 (52%), Gaps = 4/137 (2%) Frame = +3 Query: 195 SEELAQESLEWIRVITGEPENISGDMDNFYEVLKDGTLLCKLVNTIQPNMVKKVNVSKMA 374 + +L +E+ EWI N D+ + L+ G +LC++ ++ S M Sbjct: 2 TSQLEKEAREWIEETLHTKLNAQLDL---LDQLQSGVILCRICKEALGANIR-YKESNMP 57 Query: 375 FKCMENINAFLEAARQL-GVPAQETFQTVDLWERQNLNSVVICLQSLGRKAGGF--GK-P 542 F MENI+AF+ A+Q+ VP+Q+ FQT DL+ER+N V+ + S R A GK Sbjct: 58 FVQMENISAFINYAQQVVHVPSQDMFQTSDLFERRNDEQVLRSIHSFSRYAAKMFPGKVR 117 Query: 543 SIGPKEAEKNVRNFSEE 593 +GPK AEK R FS + Sbjct: 118 GLGPKLAEKKPRVFSAQ 134 >SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual Length = 1489 Score = 56.0 bits (129), Expect = 4e-09 Identities = 31/101 (30%), Positives = 54/101 (53%), Gaps = 1/101 (0%) Frame = +3 Query: 213 ESLEWIRVITGEPENISGDMDNFYEVLKDGTLLCKLVNTIQPNMVKKVNVS-KMAFKCME 389 E+ +WI G G F + L++G +L LV QP+ + K+ S ++ F+ + Sbjct: 46 EAKKWIEECLGTD---LGPTSTFEQSLRNGVVLALLVQKFQPDKLIKIFYSNELQFRHSD 102 Query: 390 NINAFLEAARQLGVPAQETFQTVDLWERQNLNSVVICLQSL 512 NIN FL+ +G+P F+ D++E +NL V+ C+ +L Sbjct: 103 NINKFLDFIHGIGLPEIFHFELTDIYEGKNLPKVIYCIHAL 143 >SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr 2|||Manual Length = 962 Score = 42.3 bits (95), Expect = 5e-05 Identities = 31/138 (22%), Positives = 64/138 (46%), Gaps = 5/138 (3%) Frame = +3 Query: 114 VLNKMANNRATKSGFAAEAQRKINSKYSEELAQESL----EWIRVITGEPENISGDMDNF 281 ++ K N+ K ++ +++ ++ ++ LA L E + + E N ++D+F Sbjct: 29 MIQKFENHYVFKKDMKSKRNKEVKNQMNDWLAYGYLCSVHEAKKWLEEETNNEYQNLDDF 88 Query: 282 YEVLKDGTLLCKLVNTIQPNMVKKVNVS-KMAFKCMENINAFLEAARQLGVPAQETFQTV 458 + L +G +LC+L P + +++ + +NAF +G+ F+T Sbjct: 89 VDALVNGKVLCQLAFKYYPKLASNWKPRYQISERNTVYLNAFFHFLDFIGMFTPFRFETK 148 Query: 459 DLWERQNLNSVVICLQSL 512 DL R N+ V+ CL +L Sbjct: 149 DLVRRFNIPKVIYCLHAL 166 >SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Manual Length = 614 Score = 40.7 bits (91), Expect = 2e-04 Identities = 31/115 (26%), Positives = 57/115 (49%), Gaps = 13/115 (11%) Frame = +3 Query: 123 KMANNRATKSGFAAEAQRKINSKYSEELAQESLEWIR-VITGEPE-----NISGDMDNFY 284 ++ R T G ++ IN EE +E ++ I V+ G+P+ I+ + F+ Sbjct: 91 EVKKGRITIKGSSSSVSHTIN----EEERREFIKHINSVLAGDPDVGSRVPINTETFEFF 146 Query: 285 EVLKDGTLLCKLVNTIQPNMVKKVNVSKMA-------FKCMENINAFLEAARQLG 428 + KDG +L KL+N P+ + + ++K FKC+EN N + +A+ +G Sbjct: 147 DQCKDGLILSKLINDSVPDTIDERVLNKQRNNKPLDNFKCIENNNVVINSAKAMG 201 Score = 32.3 bits (70), Expect = 0.055 Identities = 20/66 (30%), Positives = 35/66 (53%), Gaps = 8/66 (12%) Frame = +3 Query: 255 NISGDMDNFYEVLKDGTLLCKLVNTIQPNMV--KKVNVS------KMAFKCMENINAFLE 410 +++ + +F+ L+DG +L + + I PN V KKVN + M FK +EN N ++ Sbjct: 399 DVTPSIHDFFNNLRDGLILLQAYDKITPNTVNWKKVNKAPASGDEMMRFKAVENCNYAVD 458 Query: 411 AARQLG 428 + G Sbjct: 459 LGKNQG 464 >SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1233 Score = 27.1 bits (57), Expect = 2.1 Identities = 8/29 (27%), Positives = 19/29 (65%) Frame = -2 Query: 469 SHRSTVWNVS*AGTPSCRAASRNALMFSM 383 +++S +W + +G P+C+ + L+FS+ Sbjct: 946 NYKSGIWKLVLSGLPNCKTVFEHLLLFSL 974 >SPCC794.06 |||TDT malic acid transporter|Schizosaccharomyces pombe|chr 3|||Manual Length = 431 Score = 26.6 bits (56), Expect = 2.7 Identities = 11/30 (36%), Positives = 16/30 (53%), Gaps = 1/30 (3%) Frame = -1 Query: 248 FAGDNSDPFQRLLGKFFAVFTVDLSL-RFC 162 F GDNS + + F A+F + L + FC Sbjct: 269 FVGDNSSEYLEFVATFMALFMIGLGIFNFC 298 >SPAC26A3.12c |dhp1||5'-3' exoribonuclease Dhp1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 991 Score = 26.6 bits (56), Expect = 2.7 Identities = 10/28 (35%), Positives = 15/28 (53%) Frame = +3 Query: 387 ENINAFLEAARQLGVPAQETFQTVDLWE 470 E + AF+E A+Q G+P E W+ Sbjct: 131 EELQAFIEEAKQQGIPIDENATKKKSWD 158 >SPCC1672.12c |||DUF410 family protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 303 Score = 26.2 bits (55), Expect = 3.6 Identities = 16/47 (34%), Positives = 27/47 (57%), Gaps = 1/47 (2%) Frame = -2 Query: 259 MFSGSPVITRIHSNDSWASSSLYLLLIFLCAS-AANPDLVARLLAIL 122 ++SG+ + I S A ++Y+L ++ AS AA+ D AR L +L Sbjct: 43 LYSGAKTLFEIEQTGSAADLAIYMLDVYEKASYAASLDNKARALTLL 89 >SPAP27G11.05c |vps41||vacuolar protein sorting-associated protein Vps41|Schizosaccharomyces pombe|chr 1|||Manual Length = 886 Score = 25.8 bits (54), Expect = 4.8 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = -2 Query: 592 SSEKFLTFFSASLGPIDGLPKPPAFLPND 506 +S+ +T +S G + G +PP LPND Sbjct: 184 ASDLGITVYSTEFGKVLGRLEPPKRLPND 212 >SPAC25H1.07 |||DUF1620 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 885 Score = 25.8 bits (54), Expect = 4.8 Identities = 16/43 (37%), Positives = 25/43 (58%) Frame = -2 Query: 319 SLHNNVPSLSTS*KLSISPEMFSGSPVITRIHSNDSWASSSLY 191 S ++VP+LST IS E+F GS +I SN+ ++ + Y Sbjct: 680 SYQSDVPNLSTK---IISVELFEGSHSNEKIDSNEIYSRHNDY 719 >SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces pombe|chr 3|||Manual Length = 1526 Score = 25.4 bits (53), Expect = 6.3 Identities = 11/27 (40%), Positives = 16/27 (59%) Frame = -2 Query: 277 LSISPEMFSGSPVITRIHSNDSWASSS 197 L +SPE+FS + V RI + W S+ Sbjct: 372 LGVSPELFSQNLVRPRIKAGHEWVISA 398 >SPAC1486.03c |||RNA-binding splicing factor|Schizosaccharomyces pombe|chr 1|||Manual Length = 797 Score = 25.0 bits (52), Expect = 8.3 Identities = 8/23 (34%), Positives = 16/23 (69%) Frame = +1 Query: 157 LPQKRRERSTVNTAKNLPRSRWN 225 +PQ + + V+ AKN+P+ ++N Sbjct: 92 IPQLKNKNDEVSQAKNIPKMKFN 114 >SPBC13E7.05 |gpi14||pig-M|Schizosaccharomyces pombe|chr 2|||Manual Length = 815 Score = 25.0 bits (52), Expect = 8.3 Identities = 8/12 (66%), Positives = 8/12 (66%) Frame = +1 Query: 361 YPRWRSNAWKTL 396 YPRW N WK L Sbjct: 24 YPRWTFNKWKAL 35 >SPCC16C4.14c |sfc4||transcription factor TFIIIC complex subunit Sfc4|Schizosaccharomyces pombe|chr 3|||Manual Length = 1006 Score = 25.0 bits (52), Expect = 8.3 Identities = 10/21 (47%), Positives = 12/21 (57%) Frame = +1 Query: 382 AWKTLTHSSRQRGNSACRLKK 444 AWK L RQRGN ++K Sbjct: 169 AWKMLGECHRQRGNGRVNIEK 189 >SPAC1610.04 |mug99||meiotically upregulated gene Mug99|Schizosaccharomyces pombe|chr 1|||Manual Length = 526 Score = 25.0 bits (52), Expect = 8.3 Identities = 11/42 (26%), Positives = 18/42 (42%) Frame = +1 Query: 358 TYPRWRSNAWKTLTHSSRQRGNSACRLKKHSKPSIYGKDRTS 483 +Y W+ WK L R+ CR + S Y + +T+ Sbjct: 444 SYRSWKKEYWKELLEEGRKFERQLCR-NVFGRSSFYVQQKTA 484 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,602,132 Number of Sequences: 5004 Number of extensions: 54595 Number of successful extensions: 166 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 157 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 162 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 258201856 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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