BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0005_E15 (451 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 173 8e-45 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 98 5e-22 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 91 5e-20 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 86 3e-18 SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces pomb... 29 0.25 SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 1.3 SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 26 3.1 SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 4.1 SPBC31F10.17c |||sequence orphan|Schizosaccharomyces pombe|chr 2... 25 5.4 SPBC3B8.04c |||membrane transporter|Schizosaccharomyces pombe|ch... 25 7.1 SPAC890.05 |||ribosome biogenesis protein|Schizosaccharomyces po... 24 9.4 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 173 bits (422), Expect = 8e-45 Identities = 73/98 (74%), Positives = 86/98 (87%) Frame = +2 Query: 65 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 244 MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60 Query: 245 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAG 358 PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAG Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAG 98 Score = 38.7 bits (86), Expect = 4e-04 Identities = 17/24 (70%), Positives = 20/24 (83%) Frame = +1 Query: 364 WAKDTNTEGAELVDSVLDVVRKES 435 WAK TEGAEL D+VLDVVR+E+ Sbjct: 101 WAKGHYTEGAELADAVLDVVRREA 124 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 98.3 bits (234), Expect = 5e-22 Identities = 39/97 (40%), Positives = 67/97 (69%) Frame = +2 Query: 68 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 247 REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62 Query: 248 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAG 358 RAIL+DLEP ++++ S +G ++ P+N + ++G G Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGG 99 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 91.5 bits (217), Expect = 5e-20 Identities = 43/105 (40%), Positives = 60/105 (57%), Gaps = 2/105 (1%) Frame = +2 Query: 65 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 238 MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60 Query: 239 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGTTGPR 373 YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A R Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYAR 105 Score = 27.1 bits (57), Expect = 1.3 Identities = 16/44 (36%), Positives = 21/44 (47%) Frame = +1 Query: 298 GTFRTDIPP*QLCFRTVRRRYNWAKDTNTEGAELVDSVLDVVRK 429 G +R P QL N+A+ T G ELVD V D +R+ Sbjct: 81 GPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRR 124 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 85.8 bits (203), Expect = 3e-18 Identities = 41/109 (37%), Positives = 59/109 (54%), Gaps = 6/109 (5%) Frame = +2 Query: 65 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 226 MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60 Query: 227 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGTTGPR 373 GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A R Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYAR 109 Score = 25.8 bits (54), Expect = 3.1 Identities = 10/23 (43%), Positives = 17/23 (73%) Frame = +1 Query: 361 NWAKDTNTEGAELVDSVLDVVRK 429 N+A+ T G E++DSVL+ +R+ Sbjct: 106 NYARGHYTVGKEMIDSVLERIRR 128 >SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces pombe|chr 2|||Manual Length = 814 Score = 29.5 bits (63), Expect = 0.25 Identities = 11/24 (45%), Positives = 17/24 (70%) Frame = +1 Query: 307 RTDIPP*QLCFRTVRRRYNWAKDT 378 R D+P +L +T+R ++NWA DT Sbjct: 785 REDVPIEKLFSKTIRDKFNWAADT 808 >SPBC19G7.16 |iws1||transcription elongation factor complex subunit Iws1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 428 Score = 27.1 bits (57), Expect = 1.3 Identities = 10/34 (29%), Positives = 22/34 (64%) Frame = +2 Query: 104 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 205 N++G E+++++ +DPT A + DLQ++ + Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166 >SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizosaccharomyces pombe|chr 1|||Manual Length = 506 Score = 25.8 bits (54), Expect = 3.1 Identities = 8/18 (44%), Positives = 12/18 (66%) Frame = +2 Query: 299 GPFGQIFRPDNFVFGQSG 352 GP+G +F P F+F +G Sbjct: 157 GPYGTMFLPQQFIFDSNG 174 >SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 255 Score = 25.4 bits (53), Expect = 4.1 Identities = 13/32 (40%), Positives = 16/32 (50%) Frame = -2 Query: 357 PAPDCPKTKLSGRNICPKGPERTESMVPGSRS 262 P+ PK L R I P GPE + + GS S Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51 >SPBC31F10.17c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 135 Score = 25.0 bits (52), Expect = 5.4 Identities = 15/47 (31%), Positives = 24/47 (51%) Frame = +2 Query: 302 PFGQIFRPDNFVFGQSGAGTTGPRTLTQKALS*STQFWT*SEKNPES 442 P G++ + +FVF ++ GTT P+ +K L+ Q E ES Sbjct: 5 PLGKMIQDGDFVFQKAKKGTTIPKA-RKKTLTKDLQVQVAKELEQES 50 >SPBC3B8.04c |||membrane transporter|Schizosaccharomyces pombe|chr 2|||Manual Length = 867 Score = 24.6 bits (51), Expect = 7.1 Identities = 13/31 (41%), Positives = 15/31 (48%) Frame = -2 Query: 324 GRNICPKGPERTESMVPGSRSTRMARGTYLP 232 G NI PER + +S RMAR LP Sbjct: 106 GNNINYPTPERLQKSSASRKSGRMARSQELP 136 >SPAC890.05 |||ribosome biogenesis protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 284 Score = 24.2 bits (50), Expect = 9.4 Identities = 10/19 (52%), Positives = 12/19 (63%) Frame = +1 Query: 352 RRYNWAKDTNTEGAELVDS 408 R WAKDTN G +L+ S Sbjct: 17 RNSKWAKDTNRLGFKLLSS 35 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,698,432 Number of Sequences: 5004 Number of extensions: 31455 Number of successful extensions: 92 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 87 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 90 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 166231220 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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