Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0005_E14
(560 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 112 4e-26
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 86 4e-18
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 71 9e-14
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 68 1e-12
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 59 5e-10
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 55 6e-09
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 47 2e-06
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 38 8e-04
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 36 0.004
SPAC1002.11 |gaa1||GPI-anchor transamidase complex subunit Gaa1 ... 30 0.20
SPBC16G5.02c |||ribokinase |Schizosaccharomyces pombe|chr 2|||Ma... 28 0.82
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 27 1.4
SPCC895.07 |alp14|mtc1|Mad2-dependent spindle checkpoint compone... 27 1.9
SPAC823.10c |||mitochondrial carrier with solute carrier repeats... 26 4.4
SPBC8D2.19 |mde3||serine/threonine protein kinase Mde3|Schizosac... 25 7.6
SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces ... 25 7.6
SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|ch... 25 7.6
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 112 bits (269), Expect = 4e-26
Identities = 58/160 (36%), Positives = 91/160 (56%), Gaps = 1/160 (0%)
Frame = +2
Query: 38 VAIFLAITLLGFTLGDEIPTEDNVLVLSKSNFDSVVSTTNYILVEFYAPWCGHCKSLAPE 217
+A FLA + F E+P ++K + +++ ++V+FYAPWCGHCK+LAPE
Sbjct: 7 LAAFLAFSGGFFCASAEVPK------VNKEGLNELITADKVLMVKFYAPWCGHCKALAPE 60
Query: 218 YAKAATKLAEEESPIKLAKVDATQEQELAESYGVRGYPTLKFFRNGNPV-DYSGGRQADD 394
Y AA +L E+ I L +VD T+E +L Y +RGYPTL F+NG + YSG R+ D
Sbjct: 61 YESAADEL--EKDGISLVEVDCTEEGDLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDA 118
Query: 395 IVHWLKKKTGPPAVEVTSAEQAKELIAANPVIVLGIFADQ 514
+V +++K+ P ++ + A+ + V+ F DQ
Sbjct: 119 LVKYMRKQLLPTVKPISKDTLENFVEKADDLAVVAFFKDQ 158
Score = 93.1 bits (221), Expect = 3e-20
Identities = 47/111 (42%), Positives = 73/111 (65%), Gaps = 4/111 (3%)
Frame = +2
Query: 95 TEDNVLVLSKSNFDSVV-STTNYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLA 271
++++++VL NFD +V T +LVEFYAPWCGHCK+LAP Y K A + + ++S + +A
Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYS-DDSNVVVA 411
Query: 272 KVDATQEQELAESYGVRGYPTLKFFRNG---NPVDYSGGRQADDIVHWLKK 415
K+DAT E ++ S + G+PT+ FF+ NPV Y G R +D+ ++ K
Sbjct: 412 KIDAT-ENDI--SVSISGFPTIMFFKANDKVNPVRYEGDRTLEDLSAFIDK 459
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 85.8 bits (203), Expect = 4e-18
Identities = 40/108 (37%), Positives = 60/108 (55%), Gaps = 2/108 (1%)
Frame = +2
Query: 164 LVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQELAESYGVRGYPTLKF 343
L+EFYA WCGHCKSLAP Y + L E+ + + + K+DA ++A+ Y + G+PTL +
Sbjct: 43 LIEFYATWCGHCKSLAPVYEELGA-LFEDHNDVLIGKIDADTHSDVADKYHITGFPTLIW 101
Query: 344 F--RNGNPVDYSGGRQADDIVHWLKKKTGPPAVEVTSAEQAKELIAAN 481
F PV YS R D + ++ +KTG ++ EL + N
Sbjct: 102 FPPDGSEPVQYSNARDVDSLTQFVSEKTGIKKRKIVLPSNVVELDSLN 149
Score = 80.2 bits (189), Expect = 2e-16
Identities = 41/117 (35%), Positives = 65/117 (55%), Gaps = 4/117 (3%)
Frame = +2
Query: 86 EIPTEDNVLVLSKSNFDSVVSTTNY-ILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPI 262
+I NV+ L NFD VV +LVEFYA WCG+CK LAP Y + K+ + E +
Sbjct: 135 KIVLPSNVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTY-ETLGKVFKNEPNV 193
Query: 263 KLAKVDATQEQELAESYGVRGYPTLKFFRNGN---PVDYSGGRQADDIVHWLKKKTG 424
++ K++A ++ + V +PT+KFF + P Y G R + ++ ++ KK+G
Sbjct: 194 EIVKINADVFADIGRLHEVASFPTIKFFPKDDKDKPELYEGDRSLESLIEYINKKSG 250
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 71.3 bits (167), Expect = 9e-14
Identities = 45/144 (31%), Positives = 68/144 (47%), Gaps = 7/144 (4%)
Frame = +2
Query: 44 IFLA-ITLLGFTLGDEIPTEDNVLVLSKSNFDSVVSTTNYILVEFYAPWCGHCKSLAPEY 220
+FLA +L+ G N + L+ NF V LV FYAPWCG+CK L P Y
Sbjct: 11 LFLACFSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPWCGYCKKLVPTY 70
Query: 221 AKAATKLAEEESPIKLAKVDATQEQELAESYGVRGYPTLKFF---RNGNPV---DYSGGR 382
K A+ L P+ DA Q + + Y V+G+PT+K G+ + DY+G R
Sbjct: 71 QKLASNL-HSLLPVTAVDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDR 129
Query: 383 QADDIVHWLKKKTGPPAVEVTSAE 454
+ ++ P V++ ++E
Sbjct: 130 SYKSLQKFVSDSI-PSKVKILTSE 152
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 67.7 bits (158), Expect = 1e-12
Identities = 37/121 (30%), Positives = 65/121 (53%), Gaps = 4/121 (3%)
Frame = +2
Query: 113 VLSKSNFDSVVSTTNYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQE 292
+ S ++ S + + Y+ V+ YA WCG CK+++P +++ A+K A + AKV+ ++
Sbjct: 6 IRSYQHWISTIPKSGYLAVDCYADWCGPCKAISPLFSQLASKYASPK--FVFAKVNVDEQ 63
Query: 293 QELAESYGVRGYPTLKFFRNGNPVDYSGGR--QA--DDIVHWLKKKTGPPAVEVTSAEQA 460
+++A GV+ PT FF NG +D G QA + + K TG A+ +S+
Sbjct: 64 RQIASGLGVKAMPTFVFFENGKQIDMLTGANPQALKEKVALISSKATGTGALASSSSAPV 123
Query: 461 K 463
K
Sbjct: 124 K 124
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 58.8 bits (136), Expect = 5e-10
Identities = 27/85 (31%), Positives = 44/85 (51%)
Frame = +2
Query: 113 VLSKSNFDSVVSTTNYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQE 292
V S F S+V ++V+F+A WCG CK++AP++ + + S KVD Q
Sbjct: 5 VSDSSEFKSIVCQDKLVVVDFFATWCGPCKAIAPKF----EQFSNTYSDATFIKVDVDQL 60
Query: 293 QELAESYGVRGYPTLKFFRNGNPVD 367
E+A GV P+ ++NG ++
Sbjct: 61 SEIAAEAGVHAMPSFFLYKNGEKIE 85
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 55.2 bits (127), Expect = 6e-09
Identities = 31/101 (30%), Positives = 48/101 (47%)
Frame = +2
Query: 113 VLSKSNFDSVVSTTNYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQE 292
V S ++++ +S +V+FYA WCG CK L P KL+E+ V+A +
Sbjct: 22 VESFGDYNTRISADKVTVVDFYADWCGPCKYLKP----FLEKLSEQNQKASFIAVNADKF 77
Query: 293 QELAESYGVRGYPTLKFFRNGNPVDYSGGRQADDIVHWLKK 415
++A+ GV PT+ FR G +D G + L K
Sbjct: 78 SDIAQKNGVYALPTMVLFRKGQELDRIVGADVKTLSSLLAK 118
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 47.2 bits (107), Expect = 2e-06
Identities = 35/114 (30%), Positives = 54/114 (47%), Gaps = 2/114 (1%)
Frame = +2
Query: 32 MRVAIFLAITLLGFTLGDEIPTEDNVLVLSKSNFDSVVSTTNYILVEFYAPWCGHCKSLA 211
MR++ +A + TL +P DN L +S VS + +++Y P CG CK L
Sbjct: 10 MRISWVVAFITMVQTLVSGVPLTDNDL-------ESEVSKGTWF-IKYYLPSCGACKRLG 61
Query: 212 PEYAKAATKLAE--EESPIKLAKVDATQEQELAESYGVRGYPTLKFFRNGNPVD 367
P + K E E S +VD + +EL+ +R PTL ++NG V+
Sbjct: 62 PMWDNMVEKAKEQVEGSNFHFGEVDCS--KELSSCANIRAVPTLYLYQNGEIVE 113
Score = 38.3 bits (85), Expect = 8e-04
Identities = 19/108 (17%), Positives = 49/108 (45%)
Frame = +2
Query: 92 PTEDNVLVLSKSNFDSVVSTTNYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLA 271
PT + + ++ D+ ++ ++FY+ C C ++ + A ++ + +A
Sbjct: 278 PTGTSKALALDADIDAALTDKEGWFIQFYSSECDDCDDVSTAWYAMANRM---RGKLNVA 334
Query: 272 KVDATQEQELAESYGVRGYPTLKFFRNGNPVDYSGGRQADDIVHWLKK 415
++ + + Y ++ +PT FF+ V+Y G D+V + ++
Sbjct: 335 HINCAVSKRACKQYSIQYFPTFLFFKEEAFVEYVGLPNEGDLVSFAEE 382
>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 38.3 bits (85), Expect = 8e-04
Identities = 18/111 (16%), Positives = 55/111 (49%)
Frame = +2
Query: 194 HCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQELAESYGVRGYPTLKFFRNGNPVDYS 373
HC+ A ++ + +K+A+V+ +E+E+ + ++ +PT + F+ + + Y+
Sbjct: 208 HCEDCFHWEAVWSSITRNTDERLKMAQVNCDEEKEMCNHFHIKKFPTFRVFQGFDSIQYN 267
Query: 374 GGRQADDIVHWLKKKTGPPAVEVTSAEQAKELIAANPVIVLGIFADQSTEK 526
G + ++ + + A+++ + + + ++PV L ++ +T +
Sbjct: 268 GPLKYQQLLSYSNQVASYQAIKIEEGD-IESIENSHPVFFLVLYDFATTSE 317
Score = 27.9 bits (59), Expect = 1.1
Identities = 17/54 (31%), Positives = 25/54 (46%), Gaps = 5/54 (9%)
Frame = +2
Query: 299 LAESYGVRGYPTLKFFRNGNPVDYSG--GRQADD---IVHWLKKKTGPPAVEVT 445
LA YG + P++ RNG P+ Y R+ D I W+ + P E+T
Sbjct: 343 LANKYGAQSQPSIIAVRNGMPIVYQAITPREFRDYKRITEWINIVSSPFITELT 396
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 35.9 bits (79), Expect = 0.004
Identities = 17/57 (29%), Positives = 29/57 (50%)
Frame = +2
Query: 161 ILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQELAESYGVRGYP 331
IL+ FYAPW CK + + + A++ K++A + ++AES+ V P
Sbjct: 23 ILLNFYAPWAAPCKQMNQVF----DQFAKDTKNAVFLKIEAEKFSDIAESFDVNAVP 75
>SPAC1002.11 |gaa1||GPI-anchor transamidase complex subunit Gaa1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 581
Score = 30.3 bits (65), Expect = 0.20
Identities = 20/52 (38%), Positives = 29/52 (55%), Gaps = 5/52 (9%)
Frame = +2
Query: 26 IKMRVAIFLAITLLGFTLGDEIPTEDNVLVLS-----KSNFDSVVSTTNYIL 166
I + + ++L +T +G + +ED LVLS KS F SVVST N+ L
Sbjct: 428 ITVFIFLYLMLTFIGIIFSTFMTSEDAELVLSYDLMSKSLFISVVSTLNFSL 479
>SPBC16G5.02c |||ribokinase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 318
Score = 28.3 bits (60), Expect = 0.82
Identities = 17/43 (39%), Positives = 23/43 (53%)
Frame = +2
Query: 194 HCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQELAESYGVR 322
+C L P +AA L + +SP L VDA + L S+GVR
Sbjct: 183 YCAYLVPNEHEAAILLNQADSPATLENVDAYASKLL--SFGVR 223
>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1778
Score = 27.5 bits (58), Expect = 1.4
Identities = 15/43 (34%), Positives = 23/43 (53%)
Frame = -3
Query: 558 TALANPRKAFAFSVLWSAKIPNTMTGLAAISSLACSADVTSTA 430
T+ P+KA + W + + LA+ISS A SA + ST+
Sbjct: 667 TSKPTPKKAASLPQFWLSPRSHNTARLASISSFAKSAVMNSTS 709
>SPCC895.07 |alp14|mtc1|Mad2-dependent spindle checkpoint component
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 809
Score = 27.1 bits (57), Expect = 1.9
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = +3
Query: 225 RLPLSWQRKNLR*NWRKLMPLRNKSLPKVTELEDT 329
R P S + N+ +W K + L K K+TE++ T
Sbjct: 766 RSPFSSRANNINPDWTKAIDLAAKLKQKITEMKQT 800
>SPAC823.10c |||mitochondrial carrier with solute carrier
repeats|Schizosaccharomyces pombe|chr 1|||Manual
Length = 296
Score = 25.8 bits (54), Expect = 4.4
Identities = 15/33 (45%), Positives = 20/33 (60%)
Frame = -3
Query: 153 VVDTTLSKLDLLRTRTLSSVGISSPRVNPRRVI 55
+ TTL LDLL+TR S S P++ RR+I
Sbjct: 27 ISSTTLQPLDLLKTRCQQSQRDSLPKM-VRRII 58
>SPBC8D2.19 |mde3||serine/threonine protein kinase
Mde3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 559
Score = 25.0 bits (52), Expect = 7.6
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = +2
Query: 50 LAITLLGFTLGDEIPTEDNVLVLSKSNF 133
+AI T+GD IP+ ++L+ K F
Sbjct: 459 IAINAFNETVGDRIPSSKDILITEKIPF 486
>SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1098
Score = 25.0 bits (52), Expect = 7.6
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = +2
Query: 350 NGNPVDYSGGRQADDIVHWLKKKT 421
N N +D G +Q DD+V ++K T
Sbjct: 807 NSNVMDEQGEKQKDDVVDMVRKIT 830
>SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 778
Score = 25.0 bits (52), Expect = 7.6
Identities = 9/13 (69%), Positives = 10/13 (76%)
Frame = -2
Query: 58 YSQKYCYTHLDDP 20
YS+K Y HLDDP
Sbjct: 61 YSEKILYGHLDDP 73
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,107,223
Number of Sequences: 5004
Number of extensions: 39794
Number of successful extensions: 154
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 137
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 143
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 236012634
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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