Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0005_E13
(622 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g15980.3 68416.m02022 coatomer protein complex, subunit beta ... 172 1e-43
At3g15980.2 68416.m02021 coatomer protein complex, subunit beta ... 172 1e-43
At3g15980.1 68416.m02020 coatomer protein complex, subunit beta ... 172 1e-43
At1g52360.1 68414.m05909 coatomer protein complex, subunit beta ... 171 2e-43
At1g79990.1 68414.m09356 coatomer protein complex, subunit beta ... 169 2e-42
At4g30160.1 68417.m04289 villin, putative similar to villin 2 (... 31 0.47
At2g21390.1 68415.m02546 coatomer protein complex, subunit alpha... 31 0.81
At3g12100.1 68416.m01506 cation efflux family protein / metal to... 30 1.1
At1g24938.1 68414.m03113 hypothetical protein 30 1.4
At1g62020.1 68414.m06995 coatomer protein complex, subunit alpha... 29 1.9
At1g53325.1 68414.m06044 F-box family protein-related contains w... 29 3.3
At4g04920.1 68417.m00715 expressed protein 28 4.3
At4g28330.1 68417.m04056 expressed protein 28 5.7
At4g26080.1 68417.m03755 protein phosphatase 2C ABI1 / PP2C ABI1... 27 7.6
At1g48220.1 68414.m05383 serine/threonine protein kinase, putati... 27 7.6
>At3g15980.3 68416.m02022 coatomer protein complex, subunit beta 2
(beta prime), putative contains 7 WD-40 repeats (PF00400)
(1 weak); identical to coatomer protein complex, beta
prime (beta'-COP) protein {Arabidopsis thaliana}
(GI:9294445); similar to Coatomer beta' subunit
(Beta'-coat protein) (Beta'-COP) (p102) (SP:P35606) [Homo
sapiens]
Length = 918
Score = 172 bits (419), Expect = 1e-43
Identities = 85/207 (41%), Positives = 127/207 (61%)
Frame = +2
Query: 2 LNDKELNIISYSLLVSVLEYQTAVMRGDFETADRVLPTVPHDQRTRVAHFLEKQGFKQQA 181
L DKE N+I Y+LL+S++EY+T VMRGD + A+++LPT+P +Q VAHFLE +G + A
Sbjct: 576 LVDKEFNVIGYTLLLSLIEYKTLVMRGDLDRANQILPTIPKEQHNNVAHFLESRGMIEDA 635
Query: 182 LAVSTEPEHQFELALSLGELHXXXXXXXXXXXXXXXXXXXXXARWSKLGTXXXXXXDTEL 361
L ++T+P+++F+LA+ LG L ++W +LG +L
Sbjct: 636 LEIATDPDYKFDLAIQLGRLE---------IAKEIAEEVQSESKWKQLGELAMSSGKLQL 686
Query: 362 TKICYQNARDYSALLLFAVSIGDKELLQQVAQVSSEHGDDXIAFVAYFTLNELDSCLELL 541
+ C + A D S LLL S+GD E + ++A ++ E G + +AF+ FTL L+ CL+LL
Sbjct: 687 AEDCMKYAMDLSGLLLLYSSLGDAEGVSKLACLAKEQGKNNVAFLCLFTLGRLEDCLQLL 746
Query: 542 ISRGTISEAALFARSYVPSKVCEVVKL 622
+ I EAAL ARSY+PSKV E+V L
Sbjct: 747 VESNRIPEAALMARSYLPSKVSEIVAL 773
>At3g15980.2 68416.m02021 coatomer protein complex, subunit beta 2
(beta prime), putative contains 7 WD-40 repeats (PF00400)
(1 weak); identical to coatomer protein complex, beta
prime (beta'-COP) protein {Arabidopsis thaliana}
(GI:9294445); similar to Coatomer beta' subunit
(Beta'-coat protein) (Beta'-COP) (p102) (SP:P35606) [Homo
sapiens]
Length = 918
Score = 172 bits (419), Expect = 1e-43
Identities = 85/207 (41%), Positives = 127/207 (61%)
Frame = +2
Query: 2 LNDKELNIISYSLLVSVLEYQTAVMRGDFETADRVLPTVPHDQRTRVAHFLEKQGFKQQA 181
L DKE N+I Y+LL+S++EY+T VMRGD + A+++LPT+P +Q VAHFLE +G + A
Sbjct: 576 LVDKEFNVIGYTLLLSLIEYKTLVMRGDLDRANQILPTIPKEQHNNVAHFLESRGMIEDA 635
Query: 182 LAVSTEPEHQFELALSLGELHXXXXXXXXXXXXXXXXXXXXXARWSKLGTXXXXXXDTEL 361
L ++T+P+++F+LA+ LG L ++W +LG +L
Sbjct: 636 LEIATDPDYKFDLAIQLGRLE---------IAKEIAEEVQSESKWKQLGELAMSSGKLQL 686
Query: 362 TKICYQNARDYSALLLFAVSIGDKELLQQVAQVSSEHGDDXIAFVAYFTLNELDSCLELL 541
+ C + A D S LLL S+GD E + ++A ++ E G + +AF+ FTL L+ CL+LL
Sbjct: 687 AEDCMKYAMDLSGLLLLYSSLGDAEGVSKLACLAKEQGKNNVAFLCLFTLGRLEDCLQLL 746
Query: 542 ISRGTISEAALFARSYVPSKVCEVVKL 622
+ I EAAL ARSY+PSKV E+V L
Sbjct: 747 VESNRIPEAALMARSYLPSKVSEIVAL 773
>At3g15980.1 68416.m02020 coatomer protein complex, subunit beta 2
(beta prime), putative contains 7 WD-40 repeats (PF00400)
(1 weak); identical to coatomer protein complex, beta
prime (beta'-COP) protein {Arabidopsis thaliana}
(GI:9294445); similar to Coatomer beta' subunit
(Beta'-coat protein) (Beta'-COP) (p102) (SP:P35606) [Homo
sapiens]
Length = 909
Score = 172 bits (419), Expect = 1e-43
Identities = 85/207 (41%), Positives = 127/207 (61%)
Frame = +2
Query: 2 LNDKELNIISYSLLVSVLEYQTAVMRGDFETADRVLPTVPHDQRTRVAHFLEKQGFKQQA 181
L DKE N+I Y+LL+S++EY+T VMRGD + A+++LPT+P +Q VAHFLE +G + A
Sbjct: 576 LVDKEFNVIGYTLLLSLIEYKTLVMRGDLDRANQILPTIPKEQHNNVAHFLESRGMIEDA 635
Query: 182 LAVSTEPEHQFELALSLGELHXXXXXXXXXXXXXXXXXXXXXARWSKLGTXXXXXXDTEL 361
L ++T+P+++F+LA+ LG L ++W +LG +L
Sbjct: 636 LEIATDPDYKFDLAIQLGRLE---------IAKEIAEEVQSESKWKQLGELAMSSGKLQL 686
Query: 362 TKICYQNARDYSALLLFAVSIGDKELLQQVAQVSSEHGDDXIAFVAYFTLNELDSCLELL 541
+ C + A D S LLL S+GD E + ++A ++ E G + +AF+ FTL L+ CL+LL
Sbjct: 687 AEDCMKYAMDLSGLLLLYSSLGDAEGVSKLACLAKEQGKNNVAFLCLFTLGRLEDCLQLL 746
Query: 542 ISRGTISEAALFARSYVPSKVCEVVKL 622
+ I EAAL ARSY+PSKV E+V L
Sbjct: 747 VESNRIPEAALMARSYLPSKVSEIVAL 773
>At1g52360.1 68414.m05909 coatomer protein complex, subunit beta 2
(beta prime), putative contains 7 WD-40 repeats (PF00400)
(1 weak); similar to (SP:O55029) Coatomer beta' subunit
(Beta'-coat protein) (Beta'-COP) (p102) (SP:O55029) [Mus
musculus]; similar to GI:298096 from [Homo sapiens]
Length = 926
Score = 171 bits (417), Expect = 2e-43
Identities = 85/207 (41%), Positives = 125/207 (60%)
Frame = +2
Query: 2 LNDKELNIISYSLLVSVLEYQTAVMRGDFETADRVLPTVPHDQRTRVAHFLEKQGFKQQA 181
L DKE N+I Y+LL+S++EY+T VMRGD + A +LPT+P DQ VAHFLE +G + A
Sbjct: 576 LVDKEFNVIGYTLLLSLIEYKTLVMRGDLDKASEILPTIPKDQHNSVAHFLESRGMIEDA 635
Query: 182 LAVSTEPEHQFELALSLGELHXXXXXXXXXXXXXXXXXXXXXARWSKLGTXXXXXXDTEL 361
L ++T+P+++FELA+ LG L ++W +LG ++
Sbjct: 636 LEIATDPDYRFELAIQLGRLE---------IAQEIAVEVQSESKWKQLGELAMSSGKLQM 686
Query: 362 TKICYQNARDYSALLLFAVSIGDKELLQQVAQVSSEHGDDXIAFVAYFTLNELDSCLELL 541
+ C + A D S LLL S+GD E + ++A ++ E G + +AF+ F L +L+ CL+LL
Sbjct: 687 AEECMKYAMDLSGLLLLYSSLGDAEGVTKLATLAKEQGKNNVAFLCLFMLGKLEDCLQLL 746
Query: 542 ISRGTISEAALFARSYVPSKVCEVVKL 622
+ I EAAL ARSY+PSKV E+V L
Sbjct: 747 VESNRIPEAALMARSYLPSKVSEIVAL 773
>At1g79990.1 68414.m09356 coatomer protein complex, subunit beta 2
(beta prime), putative contains 7 WD-40 repeats (PF00400)
(1 weak); similar to Coatomer beta' subunit (Beta'-coat
protein) (Beta'-COP) (p102) (SP:P35606) [Homo sapiens];
similar to Coatomer beta' subunit (Beta'-coat protein)
(Beta'-COP) (p102) (SP:O55029) [Mus musculus]
Length = 920
Score = 169 bits (410), Expect = 2e-42
Identities = 83/207 (40%), Positives = 125/207 (60%)
Frame = +2
Query: 2 LNDKELNIISYSLLVSVLEYQTAVMRGDFETADRVLPTVPHDQRTRVAHFLEKQGFKQQA 181
L DKE N+I Y+LL+S++EY+T VMRGD E A+ VLP++P + VAHFLE +G + A
Sbjct: 575 LIDKEFNVIGYTLLLSLIEYKTLVMRGDLEQANEVLPSIPKEHHNSVAHFLESRGMTEDA 634
Query: 182 LAVSTEPEHQFELALSLGELHXXXXXXXXXXXXXXXXXXXXXARWSKLGTXXXXXXDTEL 361
L V+T+P+++FELA+ LG L ++W +LG ++
Sbjct: 635 LEVATDPDYRFELAIQLGRL---------AVAKDIAVEAQNESKWKQLGELAMSSGKLDM 685
Query: 362 TKICYQNARDYSALLLFAVSIGDKELLQQVAQVSSEHGDDXIAFVAYFTLNELDSCLELL 541
+ C ++A D S LLL S+GD + + ++A ++ E G + +AF+ F L +++ CL LL
Sbjct: 686 AEECMRHAMDLSGLLLLYSSLGDADGMMKLAALAKEQGKNNVAFLCLFMLGQVEDCLHLL 745
Query: 542 ISRGTISEAALFARSYVPSKVCEVVKL 622
+ I EAAL ARSY+PSKV E+V L
Sbjct: 746 VESNRIPEAALMARSYLPSKVSEIVAL 772
>At4g30160.1 68417.m04289 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 974
Score = 31.5 bits (68), Expect = 0.47
Identities = 18/61 (29%), Positives = 31/61 (50%), Gaps = 2/61 (3%)
Frame = +2
Query: 35 SLLVSVLE-YQTAVMRGDFET-ADRVLPTVPHDQRTRVAHFLEKQGFKQQALAVSTEPEH 208
S ++ ++E ++T R FE+ TV D R RVA L++QG + L + P+
Sbjct: 323 SQMIRIIEGFETVPFRSKFESWTQETNTTVSEDGRGRVAALLQRQGVNVRGLMKAAPPKE 382
Query: 209 Q 211
+
Sbjct: 383 E 383
>At2g21390.1 68415.m02546 coatomer protein complex, subunit alpha,
putative contains Pfam PF00400: WD domain, G-beta repeat;
similar to Coatomer alpha subunit (Alpha-coat protein)
(Alpha-COP) (HEPCOP) (HEP-COP) (SP:P53621) [Homo sapiens]
Length = 1218
Score = 30.7 bits (66), Expect = 0.81
Identities = 33/196 (16%), Positives = 80/196 (40%), Gaps = 4/196 (2%)
Frame = +2
Query: 35 SLLVSVLEY--QTAVMRGDFETADRVLPTVPHDQRTRVAH--FLEKQGFKQQALAVSTEP 202
++ ++ EY + A++R + D V+ + + Q A +L+++GF + AL +
Sbjct: 591 AITINATEYIFKLALLRKKY---DHVMSMIKNSQLCGQAMIAYLQQKGFPEVALHFVEDE 647
Query: 203 EHQFELALSLGELHXXXXXXXXXXXXXXXXXXXXXARWSKLGTXXXXXXDTELTKICYQN 382
+F LAL G + W +LG ++ + + YQ
Sbjct: 648 RIRFNLALESGNISVAVASATEINEKD---------HWYRLGVEALRQGNSRIVEFAYQQ 698
Query: 383 ARDYSALLLFAVSIGDKELLQQVAQVSSEHGDDXIAFVAYFTLNELDSCLELLISRGTIS 562
+++ L + G+ + L ++ +++ + F L ++ +++L + G +
Sbjct: 699 TKNFERLSFLYLITGNLDKLSKLMKIAEVKNNVMGQFHNALYLGDVKERVKILENAGHLP 758
Query: 563 EAALFARSYVPSKVCE 610
A + A + + + E
Sbjct: 759 LAYITASVHGLTDIAE 774
>At3g12100.1 68416.m01506 cation efflux family protein / metal
tolerance protein, putative member of the cation
diffusion facilitator (CDF) family, or cation efflux
(CE) family, PMID:11500563
Length = 393
Score = 30.3 bits (65), Expect = 1.1
Identities = 15/32 (46%), Positives = 19/32 (59%)
Frame = -1
Query: 517 FIQSEVSHKRYVIVAMFTRHLSDLL*EFFITN 422
FIQ E HK Y+IV+ T L +LL +F N
Sbjct: 206 FIQDESEHKHYLIVSAVTNLLVNLLGVWFFRN 237
>At1g24938.1 68414.m03113 hypothetical protein
Length = 317
Score = 29.9 bits (64), Expect = 1.4
Identities = 17/54 (31%), Positives = 25/54 (46%), Gaps = 2/54 (3%)
Frame = +2
Query: 71 VMRGDFET--ADRVLPTVPHDQRTRVAHFLEKQGFKQQALAVSTEPEHQFELAL 226
+ RG ET + LP+ P Q TR+ H + + QA T P+ F + L
Sbjct: 113 IPRGHLETKSSSSALPSQPARQLTRLKHLVSSSARQSQAARQLTRPKRIFRVIL 166
>At1g62020.1 68414.m06995 coatomer protein complex, subunit alpha,
putative contains Pfam PF00400: WD domain, G-beta repeat;
similar to Coatomer alpha subunit (Alpha-coat protein)
(Alpha-COP) (HEPCOP) (HEP-COP) (SP:P53621) [Homo sapiens]
Length = 1216
Score = 29.5 bits (63), Expect = 1.9
Identities = 25/154 (16%), Positives = 61/154 (39%)
Frame = +2
Query: 149 FLEKQGFKQQALAVSTEPEHQFELALSLGELHXXXXXXXXXXXXXXXXXXXXXARWSKLG 328
+L+++GF + AL + +F LAL G + W +LG
Sbjct: 630 YLQQKGFPEVALHFVEDERIRFNLALESGNISVAVASATQINEKD---------HWYRLG 680
Query: 329 TXXXXXXDTELTKICYQNARDYSALLLFAVSIGDKELLQQVAQVSSEHGDDXIAFVAYFT 508
++ + + YQ +++ L + G+ + L ++ +++ + F
Sbjct: 681 VEALRQGNSGIVEFAYQQTKNFERLSFLYLITGNLDKLSKLMKIAEVKNNVMGQFHNALY 740
Query: 509 LNELDSCLELLISRGTISEAALFARSYVPSKVCE 610
L ++ +++L + G + A + A + + + E
Sbjct: 741 LGDVKERVKILENAGHLPLAYITASVHGLNDIAE 774
>At1g53325.1 68414.m06044 F-box family protein-related contains weak
hit to TIGRFAM TIGR01640 : F-box protein interaction
domain; similar to suppressor of nim1-1 (GI:22023774)
[Arabidopsis thaliana]
Length = 168
Score = 28.7 bits (61), Expect = 3.3
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = -2
Query: 219 SSNWCSGSVDTAKACCLKPCFSRKCATLVR 130
SS W S +T K C+ PC ++K T R
Sbjct: 17 SSKWLDFSKETLKMFCILPCKAKKSTTHTR 46
>At4g04920.1 68417.m00715 expressed protein
Length = 1250
Score = 28.3 bits (60), Expect = 4.3
Identities = 13/31 (41%), Positives = 19/31 (61%)
Frame = -3
Query: 146 ARPLYVGHGGQSAELYLRFRSHLASRPSGTP 54
AR +Y HGG+ A +LR H+ S P+ +P
Sbjct: 576 ARIVYSAHGGEIAIAFLRGGVHIFSGPTFSP 606
>At4g28330.1 68417.m04056 expressed protein
Length = 149
Score = 27.9 bits (59), Expect = 5.7
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = +3
Query: 366 RYAIKMPGIIALYCSLLSVLVIKNSYSRS 452
R +K +AL C+LL+ V NSYS S
Sbjct: 120 RVLLKEGNFVALLCALLAYFVFFNSYSSS 148
>At4g26080.1 68417.m03755 protein phosphatase 2C ABI1 / PP2C ABI1 /
abscisic acid-insensitive 1 (ABI1) nearly identical to
SP|P49597 Protein phosphatase 2C ABI1 (EC 3.1.3.16)
(PP2C) (Abscisic acid- insensitive 1) {Arabidopsis
thaliana}
Length = 434
Score = 27.5 bits (58), Expect = 7.6
Identities = 13/31 (41%), Positives = 16/31 (51%)
Frame = -3
Query: 167 NPVSRGNARPLYVGHGGQSAELYLRFRSHLA 75
+P S + +Y GHGG Y R R HLA
Sbjct: 165 DPQSAAHFFGVYDGHGGSQVANYCRERMHLA 195
>At1g48220.1 68414.m05383 serine/threonine protein kinase, putative
similar to Pto kinase interactor 1 (Pti1)[Lycopersicon
esculentum] gi|3668069|gb|AAC61805
Length = 388
Score = 27.5 bits (58), Expect = 7.6
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = +2
Query: 407 LFAVSIGDKELLQQVAQVSSEHGDDXIAFVAY 502
L+ D+E L QV+ VS H ++ +A +AY
Sbjct: 98 LYPTKQPDQEFLSQVSMVSRLHHENVVALMAY 129
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,823,007
Number of Sequences: 28952
Number of extensions: 209030
Number of successful extensions: 504
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 493
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 497
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1255974912
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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