Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0005_E05
(515 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g16460.2 68416.m02097 jacalin lectin family protein contains ... 30 0.80
At3g16460.1 68416.m02098 jacalin lectin family protein contains ... 30 0.80
At4g17880.1 68417.m02665 basic helix-loop-helix (bHLH) family pr... 30 1.1
At4g19800.1 68417.m02904 glycosyl hydrolase family 18 protein si... 29 1.9
At4g34350.1 68417.m04881 LytB family protein contains Pfam profi... 28 3.2
At4g25390.2 68417.m03653 protein kinase family protein contains ... 28 4.3
At4g25390.1 68417.m03652 protein kinase family protein contains ... 28 4.3
At4g19810.1 68417.m02905 glycosyl hydrolase family 18 protein si... 28 4.3
At4g20190.1 68417.m02952 hypothetical protein 27 5.7
At2g46530.2 68415.m05803 transcriptional factor B3 family protei... 27 5.7
At2g46530.1 68415.m05802 transcriptional factor B3 family protei... 27 5.7
At5g65630.1 68418.m08256 DNA-binding bromodomain-containing prot... 27 7.5
At5g57000.1 68418.m07114 expressed protein similar to unknown pr... 27 7.5
At2g36730.1 68415.m04506 pentatricopeptide (PPR) repeat-containi... 27 7.5
At5g24120.1 68418.m02835 RNA polymerase sigma subunit SigE (sigE... 27 9.9
At4g27690.1 68417.m03981 vacuolar protein sorting-associated pro... 27 9.9
At3g07300.1 68416.m00869 eukaryotic translation initiation facto... 27 9.9
>At3g16460.2 68416.m02097 jacalin lectin family protein contains
Pfam profile: PF01419 jacalin-like lectin domain;
similar to myrosinase binding protein [Brassica napus]
GI:1711296, GI:1655824, myrosinase-binding protein
homolog [Arabidopsis thaliana] GI:2997767; contains Pfam
profile PF01419 jacalin-like lectin family
Length = 647
Score = 30.3 bits (65), Expect = 0.80
Identities = 21/70 (30%), Positives = 29/70 (41%)
Frame = +1
Query: 16 GISTTGRTWKLDSDSEISGVPPIHADEGGEAGPYTKVQGLLSYPEICAKLINPNQNGKRP 195
G ST G +W D S+ GV I+A GGE Y K + L Q+ + P
Sbjct: 259 GNSTGGTSW--DDGSDYDGVTKIYASYGGEGIQYVKFDYVKGGVTKQGVLHGKQQSRQNP 316
Query: 196 HLRKVNDPSK 225
+N P +
Sbjct: 317 REFVINHPDE 326
>At3g16460.1 68416.m02098 jacalin lectin family protein contains
Pfam profile: PF01419 jacalin-like lectin domain;
similar to myrosinase binding protein [Brassica napus]
GI:1711296, GI:1655824, myrosinase-binding protein
homolog [Arabidopsis thaliana] GI:2997767; contains Pfam
profile PF01419 jacalin-like lectin family
Length = 705
Score = 30.3 bits (65), Expect = 0.80
Identities = 21/70 (30%), Positives = 29/70 (41%)
Frame = +1
Query: 16 GISTTGRTWKLDSDSEISGVPPIHADEGGEAGPYTKVQGLLSYPEICAKLINPNQNGKRP 195
G ST G +W D S+ GV I+A GGE Y K + L Q+ + P
Sbjct: 259 GNSTGGTSW--DDGSDYDGVTKIYASYGGEGIQYVKFDYVKGGVTKQGVLHGKQQSRQNP 316
Query: 196 HLRKVNDPSK 225
+N P +
Sbjct: 317 REFVINHPDE 326
>At4g17880.1 68417.m02665 basic helix-loop-helix (bHLH) family
protein bHLH protein, Arabidopsis thaliana,
PATCHX:E255557
Length = 589
Score = 29.9 bits (64), Expect = 1.1
Identities = 21/66 (31%), Positives = 29/66 (43%), Gaps = 2/66 (3%)
Frame = +1
Query: 211 NDPSKRFGTYAFRLPDDXGEG--GFWVSYEDPDTAGNKASYVKTKNLGGVSIMDLSMDDF 384
N+ FG++AF L D GE G W+S + +G A+ V N G S +
Sbjct: 253 NNGGGEFGSWAFNLNPDQGENDPGLWISEPNGVDSGLVAAPV-MNNGGNDSTSNSDSQPI 311
Query: 385 RGLCTG 402
LC G
Sbjct: 312 SKLCNG 317
>At4g19800.1 68417.m02904 glycosyl hydrolase family 18 protein
similar to chitinase, class V GI:899342 from [Nicotiana
tabacum]
Length = 398
Score = 29.1 bits (62), Expect = 1.9
Identities = 34/137 (24%), Positives = 52/137 (37%)
Frame = +1
Query: 4 KLVLGISTTGRTWKLDSDSEISGVPPIHADEGGEAGPYTKVQGLLSYPEICAKLINPNQN 183
K VLG G W L +D +++G + GP G +SY ++ +++ N
Sbjct: 229 KAVLGFPYYGWAWTL-ADPDVNGY------DANTTGPAISDDGEISYRQLQTWIVD---N 278
Query: 184 GKRPHLRKVNDPSKRFGTYAFRLPDDXGEGGFWVSYEDPDTAGNKASYVKTKNLGGVSIM 363
G KV+D G Y + G W+ Y+ + K Y K K L G
Sbjct: 279 GAT----KVHD-DMMVGDYCYA-------GTTWIGYDSEKSIVTKVIYAKQKGLLGYFSW 326
Query: 364 DLSMDDFRGLCTGDKYP 414
+ DD L + P
Sbjct: 327 HVGGDDNSELSSAGSTP 343
>At4g34350.1 68417.m04881 LytB family protein contains Pfam profile:
PF02401 LytB protein
Length = 466
Score = 28.3 bits (60), Expect = 3.2
Identities = 17/52 (32%), Positives = 22/52 (42%)
Frame = +1
Query: 7 LVLGISTTGRTWKLDSDSEISGVPPIHADEGGEAGPYTKVQGLLSYPEICAK 162
LV+G + T L SE G+P D GP K+ L Y E+ K
Sbjct: 372 LVVGGWNSSNTSHLQEISEARGIPSYWIDSEKRIGPGNKIAYKLHYGELVEK 423
>At4g25390.2 68417.m03653 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 497
Score = 27.9 bits (59), Expect = 4.3
Identities = 17/62 (27%), Positives = 30/62 (48%)
Frame = +1
Query: 169 NPNQNGKRPHLRKVNDPSKRFGTYAFRLPDDXGEGGFWVSYEDPDTAGNKASYVKTKNLG 348
N + + +RP R+ + S R T +F + G+GGF V + + G + VK + G
Sbjct: 74 NASSSPRRPSPREFSYSSLRRATGSFSQANRLGQGGFGVVFRGTISGGENVA-VKVMDSG 132
Query: 349 GV 354
+
Sbjct: 133 SL 134
>At4g25390.1 68417.m03652 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 651
Score = 27.9 bits (59), Expect = 4.3
Identities = 17/62 (27%), Positives = 30/62 (48%)
Frame = +1
Query: 169 NPNQNGKRPHLRKVNDPSKRFGTYAFRLPDDXGEGGFWVSYEDPDTAGNKASYVKTKNLG 348
N + + +RP R+ + S R T +F + G+GGF V + + G + VK + G
Sbjct: 74 NASSSPRRPSPREFSYSSLRRATGSFSQANRLGQGGFGVVFRGTISGGENVA-VKVMDSG 132
Query: 349 GV 354
+
Sbjct: 133 SL 134
>At4g19810.1 68417.m02905 glycosyl hydrolase family 18 protein
similar to chitinase/lysozyme GI:467689 from [Nicotiana
tabacum]
Length = 379
Score = 27.9 bits (59), Expect = 4.3
Identities = 13/41 (31%), Positives = 18/41 (43%)
Frame = +1
Query: 271 GGFWVSYEDPDTAGNKASYVKTKNLGGVSIMDLSMDDFRGL 393
G W+ Y+D + K Y K + L G + DD GL
Sbjct: 318 GTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADDNSGL 358
>At4g20190.1 68417.m02952 hypothetical protein
Length = 389
Score = 27.5 bits (58), Expect = 5.7
Identities = 19/88 (21%), Positives = 34/88 (38%)
Frame = -3
Query: 402 SSAKTTKIIHGKVHDGHTAKVLCFHVTSFVSGRVRIFVRNPEATFTXIIRKAEGIGPKTF 223
S+ +K I+ DG LC ++ F G+ R +++FT R ++
Sbjct: 195 SNTANSKSINS-FEDGFKCSALCLYLPGFSKGKPVRSSRKGDSSFT---RTTTMTSSQSM 250
Query: 222 ARIVDLTKMRTLSVLVGVNKFGANFWVA 139
AR + LS + +F W +
Sbjct: 251 ARTASIRDTAVLSARASLERFECGSWTS 278
>At2g46530.2 68415.m05803 transcriptional factor B3 family protein /
auxin-responsive factor AUX/IAA-related contains Pfam
profiles: PF02309 AUX/IAA family, PF02362 B3 DNA binding
domain
Length = 514
Score = 27.5 bits (58), Expect = 5.7
Identities = 14/38 (36%), Positives = 23/38 (60%), Gaps = 1/38 (2%)
Frame = +2
Query: 146 QKFAPNLLTPTKTESVLIFVRSTILANVLG-PMPSAFL 256
+ F+P+ LTPT T+ RS ++ + G P+ S+FL
Sbjct: 263 EPFSPSALTPTPTQQQSKSKRSRPISEITGSPVASSFL 300
>At2g46530.1 68415.m05802 transcriptional factor B3 family protein /
auxin-responsive factor AUX/IAA-related contains Pfam
profiles: PF02309 AUX/IAA family, PF02362 B3 DNA binding
domain
Length = 601
Score = 27.5 bits (58), Expect = 5.7
Identities = 14/38 (36%), Positives = 23/38 (60%), Gaps = 1/38 (2%)
Frame = +2
Query: 146 QKFAPNLLTPTKTESVLIFVRSTILANVLG-PMPSAFL 256
+ F+P+ LTPT T+ RS ++ + G P+ S+FL
Sbjct: 350 EPFSPSALTPTPTQQQSKSKRSRPISEITGSPVASSFL 387
>At5g65630.1 68418.m08256 DNA-binding bromodomain-containing protein
similar to 5.9 kb fsh membrane protein [Drosophila
melanogaster] GI:157455; contains Pfam profile PF00439:
Bromodomain
Length = 590
Score = 27.1 bits (57), Expect = 7.5
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +1
Query: 169 NPNQNGKRPHLRKVNDPSKRFGTYA 243
NPN N +R + K D S FG+YA
Sbjct: 44 NPNPNFERSNSSKQCDDSSEFGSYA 68
>At5g57000.1 68418.m07114 expressed protein similar to unknown
protein (gb|AAF21159.1)
Length = 187
Score = 27.1 bits (57), Expect = 7.5
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = -2
Query: 295 LRKKPRSHLHHXHQE 251
L++KP HLHH H+E
Sbjct: 87 LKEKPPRHLHHHHKE 101
>At2g36730.1 68415.m04506 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 501
Score = 27.1 bits (57), Expect = 7.5
Identities = 12/35 (34%), Positives = 21/35 (60%), Gaps = 1/35 (2%)
Frame = -3
Query: 246 EGIGPKTFARIVDLTKMRTLSVLVGVNKFG-ANFW 145
EGIG K R+++L R+ ++++ N+F A W
Sbjct: 401 EGIGEKVKKRLIELEPKRSGNLVIVANRFAEARMW 435
>At5g24120.1 68418.m02835 RNA polymerase sigma subunit SigE (sigE) /
sigma-like factor (SIG5) identical to RNA polymerase
sigma subunit SigE [Arabidopsis thaliana] GI:4972299,
sigma-like factor [Arabidopsis thaliana] GI:4033838;
contains Pfam profiles PF04545: Sigma-70, region 4,
PF04539: Sigma-70 region 3, PF04542: Sigma-70 region 2
Length = 517
Score = 26.6 bits (56), Expect = 9.9
Identities = 16/44 (36%), Positives = 24/44 (54%), Gaps = 1/44 (2%)
Frame = +1
Query: 349 GVSIMDLSMDDFRGLCTG-DKYPILRAAKYRL*TFSLYF*KHAL 477
G DL RGL T D++ R K+RL T+ L++ +HA+
Sbjct: 305 GPKFQDLCQAGMRGLITAIDRFEPKR--KFRLSTYGLFWIRHAI 346
>At4g27690.1 68417.m03981 vacuolar protein sorting-associated
protein 26, putative / VPS26, putative similar to
vacuolar sorting protein 26 [Homo sapiens] GI:9622852;
contains Pfam profile PF03643: Vacuolar protein
sorting-associated protein 26
Length = 303
Score = 26.6 bits (56), Expect = 9.9
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = -3
Query: 411 IFISSAKTTKIIHGKVHDGHTAKVLCFHVTSFVSGRVRI 295
I S K K + K +G TA V FH +SG+V I
Sbjct: 16 ITFSDGKNRKQVPMKKENGQTALVPLFHSQDTISGKVCI 54
>At3g07300.1 68416.m00869 eukaryotic translation initiation factor
2B family protein / eIF-2B family protein similar to
SP|P49770 Translation initiation factor eIF-2B beta
subunit (eIF-2B GDP-GTP exchange factor) {Homo sapiens};
contains Pfam profile PF01008: Initiation factor 2
subunit family
Length = 407
Score = 26.6 bits (56), Expect = 9.9
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +1
Query: 334 TKNLGGVSIMDLSMDDFRGLCTGDKYPILRAA 429
T +GG+ ++D S DD C G +P + AA
Sbjct: 93 TAAMGGLDLLDASDDDDVDNCKGIGFPAMSAA 124
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,190,364
Number of Sequences: 28952
Number of extensions: 272837
Number of successful extensions: 705
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 689
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 705
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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