BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0005_D22
(269 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce... 26 1.1
SPBC15D4.09c |||cystathionine gamma-synthase |Schizosaccharomyce... 25 1.9
SPAC57A7.13 |||RNA-binding protein|Schizosaccharomyces pombe|chr... 25 2.5
SPAC16A10.03c |||zinc finger protein Pep5/Vps11 |Schizosaccharom... 24 3.2
SPCC1450.02 ||SPCC191.13|bromodomain protein|Schizosaccharomyces... 24 4.3
SPAC17G6.16c |ysh1||mRNA cleavage and polyadenylation specificit... 24 4.3
SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyc... 23 5.7
SPCC188.08c |ubp22|ubp5|ubiquitin C-terminal hydrolase Ubp22|Sch... 23 5.7
SPAC631.02 |||bromodomain protein|Schizosaccharomyces pombe|chr ... 23 5.7
SPAC3H1.10 |||phytochelatin synthetase |Schizosaccharomyces pomb... 23 5.7
SPBC342.05 |crb2|rhp9, rhp9|DNA repair protein RAD9 homolog, Rhp... 23 7.5
SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 23 9.9
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 23 9.9
>SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1242
Score = 25.8 bits (54), Expect = 1.1
Identities = 17/43 (39%), Positives = 22/43 (51%)
Frame = +2
Query: 2 LLSVYIAKGVSMSNFKPVECLNTCGDK*LRSFWRDLSLTFSNC 130
LL++ I +S+ FKP+E RSFWR L TF C
Sbjct: 816 LLALEILIFLSIKLFKPLELT--------RSFWRTLVSTFCRC 850
>SPBC15D4.09c |||cystathionine gamma-synthase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 610
Score = 25.0 bits (52), Expect = 1.9
Identities = 15/45 (33%), Positives = 20/45 (44%)
Frame = -2
Query: 241 SSIINLNLNTIAKLFHTNATKIT*YRVKYTSFNELKKTIAKCQTE 107
S +IN N TI L + N T Y Y +N K+ C+ E
Sbjct: 483 SQVINHNAETICNLLYQNPKIKTLY---YPKYNTSKEHFEACRRE 524
>SPAC57A7.13 |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 565
Score = 24.6 bits (51), Expect = 2.5
Identities = 7/17 (41%), Positives = 12/17 (70%)
Frame = +2
Query: 8 SVYIAKGVSMSNFKPVE 58
S+Y+ +G+ FKP+E
Sbjct: 325 SIYVPEGIDTDQFKPIE 341
>SPAC16A10.03c |||zinc finger protein Pep5/Vps11
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 860
Score = 24.2 bits (50), Expect = 3.2
Identities = 15/39 (38%), Positives = 22/39 (56%)
Frame = -2
Query: 238 SIINLNLNTIAKLFHTNATKIT*YRVKYTSFNELKKTIA 122
SI NL L + KLF + T++ Y++K N KK I+
Sbjct: 188 SITNLILYSPKKLFVSTTTQVMVYKIK----NNTKKVIS 222
>SPCC1450.02 ||SPCC191.13|bromodomain protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 578
Score = 23.8 bits (49), Expect = 4.3
Identities = 9/16 (56%), Positives = 12/16 (75%)
Frame = +2
Query: 89 RSFWRDLSLTFSNCFL 136
+ F D++L FSNCFL
Sbjct: 149 QEFIDDMNLMFSNCFL 164
>SPAC17G6.16c |ysh1||mRNA cleavage and polyadenylation specificity
factor complex subunit Ysh1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 775
Score = 23.8 bits (49), Expect = 4.3
Identities = 9/14 (64%), Positives = 11/14 (78%)
Frame = -2
Query: 109 EIPPKTPKLLITTS 68
E+PPK P +LIT S
Sbjct: 217 EVPPKRPDVLITES 230
>SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 987
Score = 23.4 bits (48), Expect = 5.7
Identities = 8/17 (47%), Positives = 12/17 (70%)
Frame = +2
Query: 113 LTFSNCFL*FIKTCVLD 163
+ FSNCF +I C+L+
Sbjct: 794 INFSNCFTYWIINCILE 810
>SPCC188.08c |ubp22|ubp5|ubiquitin C-terminal hydrolase
Ubp22|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1108
Score = 23.4 bits (48), Expect = 5.7
Identities = 8/20 (40%), Positives = 13/20 (65%)
Frame = -2
Query: 157 YTSFNELKKTIAKCQTEIPP 98
+T F ELKK + + ++PP
Sbjct: 155 FTQFAELKKLLCRQAPDVPP 174
>SPAC631.02 |||bromodomain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 727
Score = 23.4 bits (48), Expect = 5.7
Identities = 9/14 (64%), Positives = 11/14 (78%)
Frame = +2
Query: 95 FWRDLSLTFSNCFL 136
F D++L FSNCFL
Sbjct: 296 FIDDMNLMFSNCFL 309
>SPAC3H1.10 |||phytochelatin synthetase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 414
Score = 23.4 bits (48), Expect = 5.7
Identities = 15/50 (30%), Positives = 23/50 (46%), Gaps = 2/50 (4%)
Frame = -2
Query: 253 CYALSSIIN--LNLNTIAKLFHTNATKIT*YRVKYTSFNELKKTIAKCQT 110
C +LS I + L + L + N + VK SF+E +K + C T
Sbjct: 126 CRSLSDIEKDGVTLEEFSCLANCNGLRTITKCVKDVSFDEFRKDVISCST 175
>SPBC342.05 |crb2|rhp9, rhp9|DNA repair protein RAD9 homolog,
Rhp9|Schizosaccharomyces pombe|chr 2|||Manual
Length = 778
Score = 23.0 bits (47), Expect = 7.5
Identities = 12/38 (31%), Positives = 18/38 (47%)
Frame = -3
Query: 132 KQLLNVRLRSRQKLLSYLSPQVFKHSTGLKFDIDTPFA 19
+ L N +S+QK L +L PQ S F + F+
Sbjct: 578 RPLFNDPFKSKQKKLRHLKPQKRSKSWNQAFVVSDTFS 615
>SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1610
Score = 22.6 bits (46), Expect = 9.9
Identities = 16/44 (36%), Positives = 21/44 (47%), Gaps = 2/44 (4%)
Frame = +2
Query: 59 CLNTCGDK*--LRSFWRDLSLTFSNCFL*FIKTCVLDSISCNLS 184
CL + DK S W ++ F N I+T DS+S NLS
Sbjct: 458 CLQSVFDKNSDFASLWNEVLFGFFNDESMDIETIPFDSLSRNLS 501
>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1044
Score = 22.6 bits (46), Expect = 9.9
Identities = 8/21 (38%), Positives = 15/21 (71%)
Frame = +3
Query: 117 HLAIVFFNSLKLVYLTLYHVI 179
H ++FF+ K+ ++TL HV+
Sbjct: 314 HPDLLFFDVQKISWITLAHVV 334
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 928,339
Number of Sequences: 5004
Number of extensions: 14377
Number of successful extensions: 51
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 49
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 51
length of database: 2,362,478
effective HSP length: 61
effective length of database: 2,057,234
effective search space used: 57602552
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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