Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0005_D14
(442 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC20H4.06c |||RNA-binding protein|Schizosaccharomyces pombe|ch... 29 0.42
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 26 2.2
SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr... 26 2.2
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 26 3.0
SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|ch... 26 3.0
SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces... 26 3.0
SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|c... 25 3.9
SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 25 5.2
SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccha... 25 6.8
SPBC28F2.08c |||HRD ubiquitin ligase complex subunit |Schizosacc... 25 6.8
SPBC1604.13c |mrpl32||mitochondrial ribosomal protein subunit L3... 25 6.8
SPBC21B10.03c |||ataxin-2 homolog|Schizosaccharomyces pombe|chr ... 25 6.8
>SPAC20H4.06c |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 534
Score = 28.7 bits (61), Expect = 0.42
Identities = 14/30 (46%), Positives = 19/30 (63%), Gaps = 3/30 (10%)
Frame = -3
Query: 254 RGSAVRKSVQCS---PPLWGYVQVYNSQMP 174
+G ++ KS CS PPL G+V V+N Q P
Sbjct: 256 KGKSIIKSQNCSDGLPPLPGFVVVFNLQSP 285
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 26.2 bits (55), Expect = 2.2
Identities = 21/68 (30%), Positives = 30/68 (44%), Gaps = 5/68 (7%)
Frame = +2
Query: 2 DKESEHREG---FTALVRE--MKQALNVKPNMQLVISVLPNVNSSIYFDVPSIINLVDIV 166
D ES H EG F + E MK+ALN ++ +S ++ I FD I D V
Sbjct: 985 DYESHHGEGNVHFNVTLTEAGMKEALNESLEVKFKLSRTQATSNMIAFDASGRIKKYDSV 1044
Query: 167 NIQAFDYY 190
++Y
Sbjct: 1045 EDILTEFY 1052
>SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 780
Score = 26.2 bits (55), Expect = 2.2
Identities = 12/31 (38%), Positives = 17/31 (54%), Gaps = 1/31 (3%)
Frame = +3
Query: 312 PTNLSL-VSAPLDVRGSSIQTVKFPEFHRYT 401
PT L SA DVR +S + +FH+Y+
Sbjct: 51 PTELKARTSATFDVRSASTSPINASDFHKYS 81
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 25.8 bits (54), Expect = 3.0
Identities = 15/53 (28%), Positives = 25/53 (47%), Gaps = 2/53 (3%)
Frame = +2
Query: 230 PIYAPQNRDPLQNADAAINYWIQNGAPTHKLVLGISTTGRTWK--LDSDSEIS 382
P PQ+ + +QN + + + G P K V+ + T DSDS++S
Sbjct: 777 PESPPQSYESIQNDNELLQELLSMGFPREKAVIALEATNYDVNEVSDSDSKLS 829
>SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 589
Score = 25.8 bits (54), Expect = 3.0
Identities = 9/21 (42%), Positives = 14/21 (66%)
Frame = -2
Query: 84 CILGFTLSACFISRTRAVKPS 22
CI GF ++ + +RTR + PS
Sbjct: 325 CIAGFVVNELYTTRTRIIAPS 345
>SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1009
Score = 25.8 bits (54), Expect = 3.0
Identities = 10/22 (45%), Positives = 12/22 (54%)
Frame = -1
Query: 166 DNVDQVYDAWDVKVNGAIYVWQ 101
D VD++YDAW N WQ
Sbjct: 54 DYVDEMYDAWKKDPNSVHASWQ 75
>SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 589
Score = 25.4 bits (53), Expect = 3.9
Identities = 9/21 (42%), Positives = 14/21 (66%)
Frame = -2
Query: 84 CILGFTLSACFISRTRAVKPS 22
CI GF ++ + +RTR + PS
Sbjct: 325 CIAGFVVNEFYTTRTRIITPS 345
>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
Mms2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 25.0 bits (52), Expect = 5.2
Identities = 11/32 (34%), Positives = 16/32 (50%)
Frame = +2
Query: 125 YFDVPSIINLVDIVNIQAFDYYTPERNPKEAD 220
Y D P I+ V +N+ D T + NP + D
Sbjct: 68 YPDAPPIVTFVSRINLPGVDGETGKVNPHKID 99
>SPAC23E2.03c |ste7||meiotic suppressor protein
Ste7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 569
Score = 24.6 bits (51), Expect = 6.8
Identities = 14/39 (35%), Positives = 21/39 (53%), Gaps = 3/39 (7%)
Frame = +3
Query: 297 KMVRLPTNLSLVSAPLDVR---GSSIQTVKFPEFHRYTP 404
+++ +P NL+ PL GSS +T + PEF TP
Sbjct: 144 ELIMVPPNLA--GKPLSANSSFGSSAKTFQLPEFDSVTP 180
>SPBC28F2.08c |||HRD ubiquitin ligase complex subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 713
Score = 24.6 bits (51), Expect = 6.8
Identities = 12/40 (30%), Positives = 20/40 (50%)
Frame = +2
Query: 197 ERNPKEADYTAPIYAPQNRDPLQNADAAINYWIQNGAPTH 316
+R+P + Y + P N +NA A+NY+ + A H
Sbjct: 451 DRDPSTS-YLKTVIFPTNEQTHRNARIAMNYYSRAAARNH 489
>SPBC1604.13c |mrpl32||mitochondrial ribosomal protein subunit
L32|Schizosaccharomyces pombe|chr 2|||Manual
Length = 103
Score = 24.6 bits (51), Expect = 6.8
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = +2
Query: 35 ALVREMKQALNVKPNMQLVISVLPNVNSSIYFDVPSIINL 154
AL+R A++ K S LP+++ I+ PSI N+
Sbjct: 2 ALLRGNSLAISQKMLSVFQASALPHISLRIFISPPSIANI 41
>SPBC21B10.03c |||ataxin-2 homolog|Schizosaccharomyces pombe|chr
2|||Manual
Length = 791
Score = 24.6 bits (51), Expect = 6.8
Identities = 15/43 (34%), Positives = 18/43 (41%)
Frame = +2
Query: 185 YYTPERNPKEADYTAPIYAPQNRDPLQNADAAINYWIQNGAPT 313
Y TP Y P Y P R+P Q+ A + NG PT
Sbjct: 590 YPTPTMPYIPVGYPVPGYTPYMRNPSQHTSVAPS---PNGTPT 629
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,861,782
Number of Sequences: 5004
Number of extensions: 37600
Number of successful extensions: 122
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 119
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 122
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 160149590
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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