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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0005_C16
         (565 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to calr...    99   1e-21
At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to SP|Q...    99   2e-21
At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to simi...    91   4e-19
At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to simi...    91   4e-19
At5g07340.1 68418.m00838 calnexin, putative identical to calnexi...    66   1e-11
At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin...    66   2e-11
At2g17150.1 68415.m01980 RWP-RK domain-containing protein simila...    26   0.31 
At3g16910.1 68416.m02162 AMP-dependent synthetase and ligase fam...    27   6.5  
At2g46710.1 68415.m05828 rac GTPase activating protein, putative...    27   6.5  
At4g23150.1 68417.m03341 protein kinase family protein contains ...    27   8.7  

>At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to
           calreticulin (crt1) GI:2052379 [Arabidopsis thaliana]
          Length = 425

 Score =   99 bits (238), Expect = 1e-21
 Identities = 43/79 (54%), Positives = 55/79 (69%)
 Frame = +3

Query: 63  IDNPDYKGVWAPKQIDNPAYKGPWIHPEIDNPEYTPDSNLYKRDEICSVGLDLWQVKSGT 242
           I NP+Y G W PK+I NPAYKG W  P IDNPE+  D  LY   ++  VG++LWQVKSG+
Sbjct: 270 IPNPEYNGEWKPKKIKNPAYKGKWKAPMIDNPEFKDDPELYVFPKLKYVGVELWQVKSGS 329

Query: 243 IFNNFLFTDDPALAKEKGE 299
           +F+N L +DDP  AK+  E
Sbjct: 330 LFDNVLVSDDPEYAKKLAE 348


>At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to
           SP|Q38858 Calreticulin 2 precursor {Arabidopsis
           thaliana}
          Length = 424

 Score = 98.7 bits (235), Expect = 2e-21
 Identities = 46/90 (51%), Positives = 59/90 (65%), Gaps = 1/90 (1%)
 Frame = +3

Query: 63  IDNPDYKGVWAPKQIDNPAYKGPWIHPEIDNPEYTPDSNLYKRDEICSVGLDLWQVKSGT 242
           I NP+Y G W PKQI NP YKG W  P IDNP++  D  LY   ++  VGL+LWQVKSG+
Sbjct: 270 IPNPEYMGEWKPKQIKNPNYKGKWEAPLIDNPDFKDDPELYVFPKLKYVGLELWQVKSGS 329

Query: 243 IFNNFLFTDDPALAKE-KGEIIKKMQEGEK 329
           +F+N L  DDP  AK+   E   K+++ EK
Sbjct: 330 LFDNVLICDDPDYAKKLADETWGKLKDAEK 359


>At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to similar
           to SP|O04153 Calreticulin 3 precursor {Arabidopsis
           thaliana}
          Length = 370

 Score = 91.5 bits (217), Expect = 4e-19
 Identities = 42/92 (45%), Positives = 58/92 (63%), Gaps = 1/92 (1%)
 Frame = +3

Query: 63  IDNPDYKGVWAPKQIDNPAYKGPWIHPEIDNPEYTPDSNLYKRDEICSVGLDLWQVKSGT 242
           I N  YKG W  K+I NP YKG W +P IDNPE+  D +LY    I   G+++WQVK+G+
Sbjct: 222 IPNSAYKGPWKAKRIKNPNYKGKWKNPWIDNPEFEDDPDLYVLKSIKYAGIEVWQVKAGS 281

Query: 243 IFNNFLFTDDPALAKE-KGEIIKKMQEGEKKM 335
           IF+N L  DDPA A+    +   + +E EK++
Sbjct: 282 IFDNILICDDPAYARSIVDDYFAQHRESEKEL 313


>At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to similar
           to SP|O04153 Calreticulin 3 precursor {Arabidopsis
           thaliana}
          Length = 424

 Score = 91.5 bits (217), Expect = 4e-19
 Identities = 42/92 (45%), Positives = 58/92 (63%), Gaps = 1/92 (1%)
 Frame = +3

Query: 63  IDNPDYKGVWAPKQIDNPAYKGPWIHPEIDNPEYTPDSNLYKRDEICSVGLDLWQVKSGT 242
           I N  YKG W  K+I NP YKG W +P IDNPE+  D +LY    I   G+++WQVK+G+
Sbjct: 276 IPNSAYKGPWKAKRIKNPNYKGKWKNPWIDNPEFEDDPDLYVLKSIKYAGIEVWQVKAGS 335

Query: 243 IFNNFLFTDDPALAKE-KGEIIKKMQEGEKKM 335
           IF+N L  DDPA A+    +   + +E EK++
Sbjct: 336 IFDNILICDDPAYARSIVDDYFAQHRESEKEL 367


>At5g07340.1 68418.m00838 calnexin, putative identical to calnexin
           homolog 2 from Arabidopsis thaliana [SP|Q38798], strong
           similarity to calnexin homolog 1, Arabidopsis thaliana,
           EMBL:AT08315 [SP|P29402]; contains Pfam profile PF00262
           calreticulin family
          Length = 532

 Score = 66.5 bits (155), Expect = 1e-11
 Identities = 28/73 (38%), Positives = 45/73 (61%)
 Frame = +3

Query: 69  NPDYKGVWAPKQIDNPAYKGPWIHPEIDNPEYTPDSNLYKRDEICSVGLDLWQVKSGTIF 248
           NP YKG W+   IDNPAYKG W   +I NP+Y  +      + I ++G+++W ++ G +F
Sbjct: 325 NPAYKGKWSSPLIDNPAYKGIWKPRDIPNPDYF-ELERPNLEPIAAIGIEIWTMQDGILF 383

Query: 249 NNFLFTDDPALAK 287
           +N L + D  +A+
Sbjct: 384 DNILISKDEKVAE 396


>At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin
           homolog 1, Arabidopsis thaliana, EMBL:AT08315
           [SP|P29402]
          Length = 530

 Score = 66.1 bits (154), Expect = 2e-11
 Identities = 28/73 (38%), Positives = 45/73 (61%)
 Frame = +3

Query: 69  NPDYKGVWAPKQIDNPAYKGPWIHPEIDNPEYTPDSNLYKRDEICSVGLDLWQVKSGTIF 248
           NP YKG W+   IDNPAYKG W   +I NP+Y  + +    + I ++G+++W ++ G +F
Sbjct: 323 NPAYKGKWSSPLIDNPAYKGIWKPRDIPNPDYF-ELDRPDYEPIAAIGIEIWTMQDGILF 381

Query: 249 NNFLFTDDPALAK 287
           +N L   D  +A+
Sbjct: 382 DNILIAKDEKVAE 394


>At2g17150.1 68415.m01980 RWP-RK domain-containing protein similar
           to nodule inception protein [Lotus japonicus]
           GI:6448579; contains Pfam profile: PF02042 RWP-RK domain
          Length = 909

 Score = 25.8 bits (54), Expect(2) = 0.31
 Identities = 8/27 (29%), Positives = 14/27 (51%)
 Frame = +3

Query: 87  VWAPKQIDNPAYKGPWIHPEIDNPEYT 167
           +W P Q +  ++   W  P + N EY+
Sbjct: 148 IWVPIQQEGKSFLTTWAQPHLFNQEYS 174



 Score = 24.6 bits (51), Expect(2) = 0.31
 Identities = 8/14 (57%), Positives = 11/14 (78%)
 Frame = +3

Query: 156 PEYTPDSNLYKRDE 197
           PE+TPD   ++RDE
Sbjct: 210 PEWTPDVRFFRRDE 223


>At3g16910.1 68416.m02162 AMP-dependent synthetase and ligase family
           protein similar to AMP-binding protein GI:1903034 from
           [Brassica napus]; contains Pfam AMP-binding domain
           PF00501; identical to cDNA adenosine monophosphate
           binding protein 7 AMPBP7 (AMPBP7)  GI:20799722
          Length = 569

 Score = 27.5 bits (58), Expect = 6.5
 Identities = 20/62 (32%), Positives = 27/62 (43%)
 Frame = -1

Query: 280 RAGSSVKRKLLKMVPDLTCHKSRPTEHISSRLYRFESGVYSGLSISGWIHGPLYAGLSIC 101
           +AGSS KR LL ++ D TC        +S     +E  + +G     W   P     SI 
Sbjct: 143 KAGSSFKRPLLIVIGDHTCAPESLNRALSKGAIEYEDFLATGDPNYPW-QPPADEWQSIA 201

Query: 100 LG 95
           LG
Sbjct: 202 LG 203


>At2g46710.1 68415.m05828 rac GTPase activating protein, putative
           similar to rac GTPase activating protein 2 [Lotus
           japonicus] GI:3695061; contains Pfam profiles PF00620:
           RhoGAP domain, PF00786: P21-Rho-binding domain
          Length = 455

 Score = 27.5 bits (58), Expect = 6.5
 Identities = 14/38 (36%), Positives = 21/38 (55%)
 Frame = +3

Query: 66  DNPDYKGVWAPKQIDNPAYKGPWIHPEIDNPEYTPDSN 179
           +N D K  W  KQ  +P+ +    H EI +PE  P++N
Sbjct: 331 ENADAKARWLKKQTSDPSEEWESQHSEILSPE-KPNNN 367


>At4g23150.1 68417.m03341 protein kinase family protein contains
           Pfam domain, PF00069: Protein kinase domain
          Length = 659

 Score = 27.1 bits (57), Expect = 8.7
 Identities = 12/40 (30%), Positives = 22/40 (55%)
 Frame = +3

Query: 153 NPEYTPDSNLYKRDEICSVGLDLWQVKSGTIFNNFLFTDD 272
           +PEY        + ++ S G+ + ++ SG   N+F+ TDD
Sbjct: 506 SPEYAMRGQFSMKSDVYSFGVLVLEIISGRKNNSFIETDD 545


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,039,442
Number of Sequences: 28952
Number of extensions: 155444
Number of successful extensions: 546
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 522
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 538
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1082538160
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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